Disinfection and removal of human pathogenic bacteria in arctic waste stabilization ponds

Wastewater stabilization ponds (WSPs) are commonly used to treat municipal wastewater in Arctic Canada. The biological treatment in the WSPs is strongly influenced by climatic conditions. Currently, there is limited information about the removal of fecal and pathogenic bacteria during the short cool summer treatment season. With relevance to public health, the objectives of this paper were to determine if treatment in arctic WSPs resulted in the disinfection (i.e., removal of fecal indicator bacteria, Escherichia coli) and removal of selected human bacterial pathogens from the treated effluent. The treatment performance, with focus on microbial removal, was assessed for the one-cell WSP in Pond Inlet (Nunavut [NU]) and two-cell WSP in Clyde River (NU) over three consecutive (2012–2014) summer treatment seasons (late June-early September). The WSPs provided a primary disinfection treatment of the wastewater with a 2–3 Log removal of generic indicator E. coli. The bacterial pathogens Salmonella spp., pathogenic E. coli, and Listeria monocytogenes, but not Campylobacter spp. and Helicobacter pylori, were detected in the untreated and treated wastewater, indicating that human pathogens were not reliably removed. Seasonal and annual variations in temperature significantly (p < 0.05) affected the disinfection efficiency. Improved disinfection and pathogen removal was observed for the two-cell system in Clyde River as compared to the one-cell system in Pond Inlet. A quantitative microbial risk assessment should be performed to determine if the release of low levels of human pathogens into the arctic environment poses a human health risk.     

Contamination of marine fauna by chlordecone in Guadeloupe: evidence of a seaward decreasing gradient

Chlordecone is an organochlorine pesticide, used in the Lesser Antilles from 1972 to 1993 to fight against a banana weevil. That molecule is very persistent in the natural environment and ends up in the sea with runoff waters. The objective of the present study is to evaluate the level of contamination in several trophic groups of marine animals according to their distance from the source of pollution. Samples of suspended matter, macroalgae, herbivorous fishes, detrivorous crustaceans, zooplanktivorous fishes, first- and second-order of carnivorous fishes, and piscivorous fishes have been collected in two sites, located downstream the contaminated sites (Goyave and Petit-Bourg), in three marine habitats (coastal mangroves, seagrass beds located 1.5 km from the shoreline, and coral reefs at 3 km offshore). Animals collected in mangroves were the most contaminated (mean concentrations 193 μg kg^?1 in Goyave and 213 μg kg^?1 in Petit-Bourg). Samples from seagrass beds presented intermediate concentrations of chlordecone (85 μg kg^?1 in Goyave and 107 μg kg^?1 in Petit-Bourg). Finally, samples from coral reefs were the less contaminated (71 μg kg^?1 in Goyave and 74 μg kg^?1 in Petit-Bourg). Reef samples, collected 3 km offshore, were two to three times less contaminated than those collected in mangroves.     

Trace metal (Cd,Cu, Pb,Zn) fractionation in urban-industrial soils of Ust-Kamenogorsk (Oskemen), Kazakhstan—implications for the assessment of environmental quality

Ust-Kamenogorsk is one of the largest cities and industrial centers in Kazakhstan. Non-ferrous metallurgy (Zn–Pb smelter) has acted as a predominating industrial branch in the city since late 1940s. The industrial plants are situated directly adjacent to the residential area of the city which creates grievous ecotoxicological hazard. In the present paper, we aimed at assessing the trace metal pollution of top soils in Ust-Kamenogorsk and its potential threats to the local population. The top soils were sampled at 10 sites throughout the city center. We determined the physical and chemical properties of soils as well as the contents of Cd, Cu, Pb, and Zn. In addition, the soil samples were subjected to a five-step sequential extraction to ascertain the fractionation of trace metals. On this basis, we calculated the geoaccumulation index (I_geo) and pollution load index (PLI) and assessed bioavailability of the elements. From our data, it emerged that the soils displayed a strong polymetallic pollution. PLI was as high as 33.4. Throughout the city, the trace metal contents exceeded the geochemical background and allowable values for residential, recreational, and institutional areas. The I_geo obtained were 3.7–6.5 for Cd, 1.5–4.7 for Cu, 2.8–5.7 for Pb, and 2.6–4.6 for Zn. The soils in Ust-Kamenogorsk displayed extremely high contamination with Cd, moderate to strong contamination with Pb and Zn, and low to moderate contamination with Cu. Cd and Pb were found to be the most bioavailable elements. The mobility of trace metals in the soils changed in the order Cd > Pb > Zn > Cu.     

Development of an efficient and sensitive analytical method for the determination of copper at trace levels by slotted quartz tube atomic absorption spectrometry after vortex-assisted dispersive liquid-liquid microextraction in biota and water samples using a novel ligand

This study describes the determination of trace levels of copper by slotted quartz tube atomic absorption spectrometry after dispersive liquid-liquid microextraction. A ligand synthesized from the reaction of salicylaldehyde and 1-naphthylamine was used to form coordinate copper complex prior to extraction. All parameters that influence the output of complex formation, extraction, and instrumental measurement were optimized to enhance the absorbance signal of copper. Under the optimum conditions, about 104-fold enhancement in sensitivity was recorded over the conventional flame atomic absorption spectrometer, corresponding to a 0.51 ng/mL detection limit. The percent relative standard deviation calculated for the lowest concentration (4.8%) indicated high precision for the experimental procedure. Accuracy and applicability of the optimum method were determined by performing spiked recovery tests on urine, lake water, and mineral water samples. Satisfactory recovery results were obtained between 82.2 and 106.3% at four different concentrations. Matrix matching method was also performed to increase the accuracy of quantification, and the percent recovery calculated for 175 ng/mL was 105.14%.     

Quantification of forest fragmentation in pre- and post-establishment periods,inside and around Apuseni Natural Park,Romania

Protected areas (PAs) represent real cornerstones in the conservation of biodiversity and natural habitats. Their protection must be a priority today for each society. The enhanced socio-economic requirements increase the pressure upon protected areas, and the effect can result in a reduction of biodiversity. The aim of this study is to quantify forest fragmentation in the pre- (1986–2002) and post-establishment (2002–2016) periods, inside and around (buffer 1, buffer 2 and buffer 3) Apuseni Natural Park (ANP), Romania, using a series of classified Landsat satellite images and six landscape metrics. The results show that forest fragmentation occurred both in the pre- and post-establishment periods, inside and around ANP. Inside the park, the deforestation rate increased four times, from 0.03% year^?1 in the pre-establishment period to 0.14% year^?1 in the post-establishment period. Around the park, the deforestation rate decreased from 0.31% year^?1 in the pre-establishment period (buffer 1) to 0.10% year^?1 in the post-establishment period (buffer 1). Forest fragmentation resulted in an increase in the patch density and edge density, and a decrease in the total core area and mean patch size leading to isolation of patches and fragmentation of landscape, especially in ANP in the post-establishment period. Several measures can be taken in order to stop forest fragmentation inside and around ANP, including the education of buffer-zone communities, reforestation, enforcement of park regulations, and forest legislation.     

The distribution and enrichment characteristics of copper in soil and <Emphasis Type="Italic">Phragmites australis</Emphasis> of Liao River estuary wetland

The aims of the present investigation were to reveal the distribution and enrichment characteristics of copper in soil and Phragmites australis of Liao River estuary wetland. The concentrations of copper in root, stem, leaf, and ear of Phragmites australis as well as in soil were determined to study the absorption capacity of copper by wild Phragmites australis of Liao River estuary wetland. The study was carried out at test pool of the Shenyang Agricultural University, and the experimental materials (soil, irrigating water and Phragmites australis) were derived from Liao River estuary wetland. The concentrations of copper in soil and Phragmites australis were 16.4441 to 49.0209 mg/kg and 0.8621 to 89.5524 mg/kg, respectively. The results indicated that the enrichment coefficients of copper in different tissues of Phragmites australis changed with the growth of Phragmites australis. The results revealed that the enrichment coefficients of copper in the whole Phragmites australis were greater than 1 at each growing stage of the Phragmites australis. The results also showed that the transfer coefficients of Phragmites australis to copper changed with the growth of Phragmites australis. The results revealed that the Phragmites australis had a good removal effect on copper from soil and had some characteristics of copper hyperaccumulator.     

A synoptic survey of select wastewater-tracer compounds and the pesticide imidacloprid in Florida’s ambient freshwaters

Current wastewater treatment technologies do not remove many unregulated hydrophilic compounds, and there is growing interest that low levels of these compounds, referred to as emerging contaminants, may impact human health and the environment. A probabilistic-designed monitoring network was employed to infer the extent of Florida’s ambient freshwaters containing the wastewater (Includes reuse water, septic systems leachate, and wastewater treatment effluent.) indicators sucralose, acetaminophen, carbamazepine, and primidone and those containing the widely used pesticide imidacloprid. Extent estimates with 95% confidence bounds are provided for canals, rivers, streams, small and large lakes, and unconfined aquifers containing ultra-trace concentrations of these compounds as based on analyses of 2015 sample surveys utilizing 528 sites. Sucralose is estimated to occur in greater than 50% of the canal, river, stream, and large lake resource extents. The pharmaceuticals acetaminophen, carbamazepine, and primidone are most prevalent in rivers, with approximately 30% of river kilometers estimated to contain at least one of these compounds. Imidacloprid is estimated to occur in 50% or greater of the canal and river resource extents, and it is the only compound found to exceed published toxicity or environmental effects standards. Geospatial analyses show sucralose detection frequencies within Florida’s drainage basins to be significantly related to the percentage of urban land use (R²?=?0.36, p?<?0.001), and imidacloprid detection frequencies to be significantly related to the percentage of urban and agricultural land use (R²?=?0.47, p?<?0.001). The extent of the presence of these compounds highlights the need for additional emerging contaminant studies especially those examining effects on aquatic biota.     

Carbon and nitrogen mineralization in Vertisol as mediated by type and placement method of residue

Selection of appropriate residue application method is essential for better use of biomass for soil and environmental health improvement. A laboratory incubation experiment was conducted for 75 days to investigate C and N mineralization of residues of soybean (Glycine max L.), chickpea (Cicer arietinum L.), maize (Zea mays L.), and wheat (Triticum aestivum L.) placed on the soil surface and incorporated into the soil. The residue of soybean and chickpea had a greater decomposition rate than that of maize and wheat, despite of their placements. Higher rate of decomposition of the residue of soybean and chickpea was recorded when it was kept on the soil surface while soil incorporation of residue of wheat and maize resulted in faster decomposition. Therefore, these findings could be used as guidelines for management of crop residue application in farmland to improve soil and environmental quality.     

Organochlorine contaminants in the Vistula Lagoon sedimentation zone as possible source of lagoon recontamination

The presented results include decade of monitoring of the Vistula Lagoon waters and have been supplemented by the determination of chlorinated compounds, as well as on concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in the sedimentation zone. Monitoring of river waters entering the Polish part of the lagoon and the lagoon waters confirmed the presence of plant protection chemical; the largest contributors has lindane (34%) and DDT_total (21%); the same as for sediments were dominate lindane (19%) and DDT_total (14%) within pp-DDT isomer dominate (13%). In the lagoon water, PCDD/Fs were determined within a range of 1.5–5.6 ng dm^?3, leading to average toxicity of 0.18?±?0.13 ng TEQ·dm^?3. In sediments, their concentrations fell within a range of 22.7–405.7 ng kg^?1 dw and the average toxicity of the lagoon sediments was set at 5.00?±?1.98 ng TEQ·kg^?1 dw. Both in water and sediments, the greatest share among PCDD/Fs has octa-chlorodibenzodioxin. Due to the hydromorphological conditions of the lagoon, the waters are mixed to the bottom causing the surface layer of sediment to become remobilized—this is suggested as the key factor when it comes to water recontamination and increased access of POPs to marine organisms.     

Intra-monsoonal variation of zooplankton population in the Sundarbans Estuarine System,India

The present study was conducted during July 2013 (early phase of monsoon or EM) and September 2013 (later phase of monsoon or LM) to ascertain the intra-monsoonal variation on zooplankton, by selecting 15 study stations in the river Saptamukhi, one of the main estuaries in the Sundarbans Estuarine System (SES). In 2013, SES experienced an unusually high monsoonal rainfall also exacerbated by cloud burst event at Himalayan region (upper stretches of SES) which tremendously increased the river runoff. The present work was aimed to decipher the effect of this unusual precipitation during the monsoon season on zooplankton assemblages along with different hydrological parameters. The abundance of zooplankton was recorded as lower during EM compared to LM. Altogether, 56 zooplankton taxa were identified with copepods forming the predominant population. Thirty-three copepod species were reported with 25 calanoid species forming the bulk of the biomass followed by 5 and 3 species of cyclopoids and harpacticoid, respectively. A combination of multivariate cluster analysis, biotic indices, and canonical correspondence analysis revealed noticeable alterations in the zooplankton community structure across the spatio-temporal scale. Furthermore, significant intra-monsoonal changes in zooplankton population correlated with several hydrological parameters were clearly noticed. Paracalanus parvus, Bestiolina similis and Oithona similis were observed to be the most dominant copepod species in both sampling periods. The result of the present study provides new insight on estuarine zooplankton community after unusual rainfall during monsoon season, and provides further evidence to support the conservation and management of the SES ecosystem.