The effect of NADH on different human and mouse cell lines

Part of the work was supported by the Bundesministerium für Forschung und Technologie, the Deutsche Forschungsgemeinschaft, the Fonds der Chemischen Industrie, and from the Carl Zeiss, Hoechst, and Ernst Leitz companies. Special thanks are due to Dr. K. Wahn, Department of Virology, University of Giessen, for conducting the electron microscope and phase-contrast investigations, to Prof. G. Sauer, German Cancer Research Center, Heidelberg, and Dr. W. R. Willems, Department of Medical Virology, University of Giessen, for supplying the different cell lines.     

Rapid prediction of disinfection by-product formation potential by fluorescence

Recent drinking water regulations have lowered the disinfection by-product standards as well as added new disinfection by-products for regulation. Natural organic matter (NOM) plays a major role in the formation of undesirable organic by-products following disinfection/oxidation of drinking water. It is suspected that most precursors to disinfection by-products are humic, although nonhumic substances are also suspected of contributing to these by-products. Many of the disinfection by-products have adverse health effects in humans (i.e., carcinogenic or mutagenic effects). The primary chlorinated disinfection by-products of concern include trihalomethanes, haloacetic acids, and haloacetonitrile. Fluorescence spectroscopy was used to study humic and fulvic acids. The two fractions of humic substances, humic and fulvic acids, were characterized by a double-peak phenomena in an overlapping fluorescing region. Disinfection by-product formation potentials of humic and fulvic acids have been correlated with total organic carbon, UV absorbance at 254 nm, specific absorbance and fluorescence. River humic and fulvic acid was found to have the highest reactivity to disinfection by-product formation as compared to soil and peat humic and fulvic acid. Fluorescence spectroscopy has shown to be a rapid and predictive tool for disinfection by-products formation potential of humic and fulvic acids.     

Chorionic villus sampling: Improved method for preparation of karyotypes after short-term incubation

A method for the isolation and trypsin-Giemsa banding of metaphases obtained after short-term incubation (48 h) of cytotrophoblast cells from chorionic villus sample is described. A new slide-making instrument, developed expressly to enhance the spreading of chromosomes from metaphases released from small tissue pieces, is responsible for the increase yield of analysable metaphases in this protocol.