Multivariate identification of plant functional response and effect traits in an agricultural landscape

Plant functional traits have been proposed as a linkage between the environmental control of vegetation and ecosystem function. Identification of traits that mediate the response of plant species to the environment is well established, but the identification of effect traits and the linkage between the two sets is less developed. This was attempted for a study of eight contrasting land uses in a marginal agricultural landscape where data on vegetation, management controls of the disturbance regime, and soil characteristics, including nitrogen release, were measured simultaneously with measures of ecosystem function such as litter decomposition rates and primary productivity on 30 sites. Trait data were assembled from databases, and an iterative multivariate approach using the three table (species, trait, environment) method RLQ was employed to identify a parsimonious set of traits that predict plant species responses to the environment and a parsimonious set of traits that link vegetation to ecosystem function. The lists of response and effect traits were similar, and where differences were observed, traits were usually highly correlated with at least one trait in the other list. This approach identified a small number of traits (canopy height, leaf dry matter content, leaf size, and specific leaf area) that provide a means of linking vegetation responses to environmental change with changes in ecosystem function. Other response traits included vegetative spread strategy, start of flowering, and seed terminal velocity, but within the system studied these traits were all significantly correlated to the traits shared between the response and effect lists.     

Directional changes in the species composition of a tropical forest

Long-term studies have revealed that the structure and dynamics of many tropical forests are changing, but the causes and consequences of these changes remain debated. To learn more about the forces driving changes within tropical forests, we investigated shifts in tree species composition over the past 25 years within the 50-ha Forest Dynamics Plot on Barro Colorado Island (BCI), Panama, and examined how observed patterns relate to predictions of (1) random population fluctuations, (2) carbon fertilization, (3) succession from past disturbance, (4) recovery from an extreme El Ni?o drought at the start of the study period, and (5) long-term climate change. We found that there have been consistent and directional changes in the tree species composition. These shifts have led to increased relative representations of drought-tolerant species as determined by the species' occurrence both across a gradient of soil moisture within BCI and across a wider precipitation gradient from a dry forest near the Pacific coast of Panama to a wet forest near its Caribbean coast. These nonrandom changes cannot be explained by stochastic fluctuations or carbon fertilization. They may be the legacy of the El Ni?o drought, or alternatively, potentially reflect increased aridity due to long-term climate change. By investigating compositional changes, we increased not only our understanding of the ecology of tropical forests and their responses to large-scale disturbances, but also our ability to predict how future global change will impact some of the critical services provided by these important ecosystems.     

Social parasitism of queens and workers in the Cape honeybee (<Emphasis Type="Italic">Apis mellifera capensis</Emphasis>)

Workers of a queenless honeybee colony can requeen the colony by raising a new queen from a young worker brood laid by the old queen. If this process fails, the colony becomes hopelessly queenless and workers activate their ovaries to lay eggs themselves. Laying Cape honeybee workers (Apis mellifera capensis) produce female offspring as an additional pathway for requeening. We tested the frequency of successful requeening in ten hopelessly queenless colonies. DNA genotyping revealed that only 8% of all queens reared in hopelessly queenless colonies were the offspring of native laying worker offspring. The vast majority of queens resulted from parasitic takeovers by foreign queens (27%) and invading parasitic workers (19%). This shows that hopelessly queenless colonies typically die due to parasitic takeovers and that the parasitic laying workers are an important life history strategy more frequently used than in providing a native queen to rescue the colony. Parasitism by foreign queens, which might enter colonies alone or accompanied by only a small worker force is much more frequent than previously considered and constitutes an additional life history strategy in Cape honeybees.     

Spatial cross-correlation of undisturbed,natural shortleaf pine stands in northern Georgia

In this study a cross-correlation statistic is used to analyse the spatial relationship among stand characteristics of natural, undisturbed shortleaf pine stands sampled during 1961–72 and 1972–82 in northern Georgia. Stand characteristics included stand age, site index, tree density, hardwood competition, and mortality. In each time period, the spatial cross-correlation statistic was used to construct cross-correlograms and cumulative cross-correlograms for all significant pairwise combination of stand characteristics. Both the cross-correlograms and cumulative cross-correlograms identified small-scale clustering and weak directional gradients for different stand characteristics in each time period. The cumulative cross-correlograms, which are based on inverse distance weighting were more sensitive in detecting small-scale clustering than the cross-correlograms based on a 0–1 weighting. Further analysis suggested that the significant cross-correlation observed among basal area growth and other stand characteristics were due, in a large part, on a subset of sample plots located in the northern part of the state, rather than regional or broad-scale variation as first thought. The ability to analyse the spatial relationship between two or more response surfaces should provide valuable insight in the development of ecosystem level models and assist decision makers in formulating pertinent policy on intelligent multiresource management.     

The evaluation of an analytical method for polychlorinated terpenes (PCC) in biological samples using an internal standard

The quantitative analysis of PCC (Toxaphene) residues in biological samples using gas chromatography with electron capture detection has been evaluated. Dechlorane 603, a chloroalicyclic compound is used as internal standard and modifications of the injection system for proper evaporation of this high-boiling compound is described. In the cleanup procedure PCB (and 1 – 3 % of the PCC) are removed by adsorption chromatography. Chlordane components and DDT compounds remaining in the PCC fraction constitute 5 – 10 % and 13 – 16 %, respectively, of the peak area in the chromatograms of the samples investigated. The quantitative results are influenced by the number of PCC-peaks chosen for the calculations and have to be specified together with the PCC-levels reported. The recoveries of PCC from spiked samples (20 ug PCC per gram crude fat above “natural” level) are 61 – 98 %, the lower figures obtained with samples containing low levels of PCC-residues. The practical limit of detection is about 10 ng PCC per g extracted fat.     

Swedish-German actinide migration experiment at ÄSPÖ hard rock laboratory

Within the scope of a bilateral cooperation between Svensk Kärnbränslehantering (SKB) and Forschungszentrum Karlsruhe, Institut für Nukleare Entsorgung (FZK-INE), an actinide migration experiment is currently being performed at the Äspö Hard Rock Laboratory (HRL) in Sweden. This paper covers laboratory and in situ investigations on actinide migration in single-fractured granite core samples. For the in situ experiment, the CHEMLAB 2 probe developed by SKB was used. The experimental setup as well as the breakthrough of inert tracers and of the actinides Am, Np and Pu are presented. The breakthrough curves of inert tracers were analyzed to determine hydraulic properties of the fractured samples. Postmortem analyses of the solid samples were performed to characterize the flow path and the sorbed actinides. After cutting the cores, the abraded material was analyzed with respect to sorbed actinides. The slices were scanned optically to visualize the flow path. Effective volumes and inner surface areas were measured.In the experiments, only breakthrough of Np(V) was observed. In each experiment, the recovery of Np(V) was ≤40%. Breakthrough of Am(III) and Pu(IV) as well as of Np(IV) was not observed.     

Release from bats: genetic distance and sensoribehavioural regression in the Pacific field cricket, Teleogryllus oceanicus

The auditory thresholds of the AN2 interneuron and the behavioural thresholds of the anti-bat flight-steering responses that this cell evokes are less sensitive in female Pacific field crickets that live where bats have never existed (Moorea) compared with individuals subjected to intense levels of bat predation (Australia). In contrast, the sensitivity of the auditory interneuron, ON1 which participates in the processing of both social signals and bat calls, and the thresholds for flight orientation to a model of the calling song of male crickets show few differences between the two populations. Genetic analyses confirm that the two populations are significantly distinct, and we conclude that the absence of bats has caused partial regression in the nervous control of a defensive behaviour in this insect. This study represents the first examination of natural evolutionary regression in the neural basis of a behaviour along a selection gradient within a single species.     

The spatial and temporal change of cropland in the Scandinavian Peninsula during 1875–1999

Land use and land cover change (LUCC) has emerged as an important issue of global change with significant influences on the geochemical cycle and regional climate change. Understanding the historical changes of land use and analyzing the environmental effects of LUCC make such reconstructions important. Based on historical statistics, this article reconstructs cropland area at the municipality level (härad/kommun) in the Scandinavian Peninsula during 1875–1999. The total acreage of cropland increased 36.84 % during 1875–1930, kept stable during 1930–1950, and decreased 14.25 % during 1950–1999. The croplands of Sweden and Norway both increased before 1950 and changed in different ways during 1950–1999 when the cropland decreased by 19.79 % in Sweden but increased by 9.63 % in Norway. The counties in the south Scandinavian Peninsula owned most of the cropland, with a cropland proportion of over 20 %, and experienced relatively obvious cropland changes. Growth centers with a rate of increase of over 5 % during 1875–1910 were found in Skåne, Stockholm, the Uppsala counties in Sweden, and the area around Oslo county in Norway. The general cropland distribution showed almost no change during this period, which mainly reflects the suitability of the natural conditions for agriculture. Multi-social factors co-impacted land use activities and induced temporal and spatial variations of the cropland. These factors included food supply, world trade, wars, agriculture policies, the economic and political environment, urbanization, and national differences. Compared with the widely used global land use dataset Historical Database of the Global Environment (HYDE), this study shows different cropland change curves before 1950. This article is a case study showing that the hindcasting model of the HYDE dataset has difficulty estimating multi-determined cropland change in the Scandinavian Peninsula, and the empirical study contributes to the improvement of the accuracy of historical land use data at the regional level.