Heteroplasmy in a deep-sea protobranch bivalve suggests an ancient origin of doubly uniparental inheritance of mitochondria in Bivalvia

Most metazoan species have strict maternal inheritance of the mitochondrial genome. In bivalves, a unique inheritance pattern called doubly uniparental inheritance (DUI) occurs in at least seven bivalve families. In this system of mitochondrial inheritance, males inherit and carry mtDNA from both parents, while females only carry mtDNA from the mother. Here, we present evidence of mitochondrial heteroplasmy in deep-sea protobranch bivalves. Divergent 16S rRNA and cytochrome b sequences were obtained within individuals of Ledella ultima. Ledella sublevis also exhibited divergent 16S sequences. Levels of divergence between 16S sequences within individuals were 27 and 15 % for each species, respectively. Ratios of homoplasmic to heteroplasmic individuals were not significantly different from 1:1, in agreement with sex ratios in protobranchs. The results provide the first evidence for mitochondrial heteroplasmy in the protobranchs and suggest DUI might have evolved much earlier in the evolution of the Bivalvia than previously thought.     

Recycling in Jerusalem: right or privilege?

This study analyses disparities in the provision of environmental services in Jerusalem, specifically through the distribution of recycling bins. The allocation of recycling bins in Jerusalem is uneven. Some neighbourhoods have more than their share of infrastructure, while others do not have any recycling bins at all. The spatial distribution of recycling bins throughout the city is analysed across the ethno-religious and socioeconomic divides, enabling us to determine whether the opportunity to recycle is a right or a privilege. The findings demonstrate that the Arab areas are literally not serviced. Ultra-Orthodox (Haredi) Jewish neighbourhoods are discriminated against unless socioeconomic levels and population size are controlled. Therefore, waste-management policies reproduce social inequalities resulting in environmental injustice. Due to the fact that recycling in Jerusalem is voluntary and not obligatory, and that in general the public wants to recycle, the opportunity to recycle is indeed a privilege.     

Impacts of golf courses on macroinvertebrate community structure in Precambrian shield streams

The influence of golf course operation on benthic macroinvertebrate communities in Precambrian Shield streams was evaluated using rapid bioassessment and the reference condition approach. Streams were sampled for water chemistry and invertebrates in 1999 and 2000, six on operational golf courses, and seven in forested reference locations. Correspondence analysis (CA) was used to determine the major patterns in the macroinvertebrate taxa, and canonical correspondence analysis (CCA) was used to evaluate relationships with environmental variables. The reference streams were used to define the normal range of variation for a variety of summary indices to evaluate the golf course streams. In all cases, golf course streams were higher in nutrients and dissolved ions and more alkaline than the forested reference streams. There was considerable variability in the macroinvertebrate fauna from the golf course streams, which was related to differences in golf course land management practices and to the potential influence of highway runoff. Of the management practices evaluated, fertilizer application rates in particular were important, as was the presence of ponds upstream on the course. Invertebrate taxa with higher abundances in golf course streams included Turbellaria, Isopoda, Amphipoda, Zygoptera, and Trombidiformes. Taxa more common in the reference streams included Ephemeroptera, Megaloptera, Culicidae, and Plecoptera. There were marked differences in the overall benthic macroinvertebrate community in three of the six golf course streams studied relative to the forested reference streams, suggesting that golf course land management on the Precambrian Shield can be associated with significant differences in macroinvertebrate community structure.     

Failing to Site Hazardous Waste Facilities Voluntarily: Implications for the Production of Sustainable Goods

A current approach to the process of siting hazardous waste facilities is to seek 'willing host' communities that will not oppose but rather voluntarily accept risky facilities. Voluntary siting strategies have been put forward as the solution to hazardous waste location problems, and there have indeed been a small number of siting successes achieved voluntarily. This paper argues that, despite claims about voluntary processes, there are indications that few 'willing host' sites will be found in response to the many that are proposed. Instead, it suggests that the limited success of siting attempts, voluntary or otherwise, signals a new phase in hazardous waste management, a shift in focus from where to site a facility to whether a good is worth producing at all if its wastes are not siteable. A characteristic of this phase is the growing influence that the disposal potential of the generated waste can have on future management decisions to produce a good. In practice, siting failure may be one of the critical turning points on the road to sustainable production. It can accomplish what technology and environmental assessments have generally been unable to do: foresee the sustainability of a good. In this way, siteability can be seen as one indicator of sustainable production. A good can be considered sustainable if the wastes associated with its production are siteable.     

Evaluating Spatial Design Techniques for Solving Land-use Allocation Problems

This study examines the use of spatial optimization techniques for multi-site land-use allocation problems (MLUA). 'Multi-site' refers to the problem of allocating more than one land-use type in an area, which are difficult problems as they involve multiple stakeholders with conflicting goals and objectives. Spatial optimization methods consist of (1) an optimization model and (2) an algorithm to solve the model. This study demonstrates a goal-programming model to solve the MLUA problem. The model is solved using both simulated annealing and genetic algorithms. Special attention has been given to introduce a spatial compactness objective in the model. It is shown that the compactness objectives in the optimization model generate compact patches of the same land use for using both the simulated annealing procedure and the genetic algorithm. In addition, it appears that using the proper settings of the compactness objectives, connectivity between patches of land use is promoted. The method is tested for a fictive study and then demonstrated for a real case study, both measuring 20 × 20 cells. The genetic algorithm generally performs better than simulated annealing in terms of solution time and achieving compactness.     

Daily mortality in the Philadelphia metropolitan area and size-classified particulate matter

Time-series of daily mortality data from May 1992 to September 1995 for various portions of the seven-county Philadelphia, PA, metropolitan area were analyzed in relation to weather and a variety of ambient air quality parameters. The air quality data included measurements of size-classified PM, SO4(2-), and H+ that had been collected by the Harvard School of Public Health, as well as routine air pollution monitoring data. Because the various pollutants of interest were measured at different locations within the metropolitan area, it was necessary to test for spatial sensitivity by comparing results for different combinations of locations. Estimates are presented for single pollutants and for multiple-pollutant models, including gaseous pollutants and mutually exclusive components of PM (PM2.5 and coarse particles, SO4(2-) and non-SO4(2-) portions of total suspended particulate [TSP] and PM10), measured on the day of death and the previous day. We concluded that associations between air quality and mortality were not limited to data collected in the same part of the metropolitan area; that is, mortality for one part may be associated with air quality data from another, not necessarily neighboring, part. Significant associations were found for a wide variety of gaseous and particulate pollutants, especially for peak O3. Using joint regressions on peak O3 with various other pollutants, we found that the combined responses were insensitive to the specific other pollutant selected. We saw no systematic differences according to particle size or chemistry. In general, the associations between daily mortality and air pollution depended on the pollutant or the PM metric, the type of collection filter used, and the location of sampling. Although peak O3 seemed to exhibit the most consistent mortality responses, this finding should be confirmed by analyzing separate seasons and other time periods.