全文获取类型
收费全文 | 20374篇 |
免费 | 181篇 |
国内免费 | 202篇 |
专业分类
安全科学 | 534篇 |
废物处理 | 987篇 |
环保管理 | 2424篇 |
综合类 | 2675篇 |
基础理论 | 5291篇 |
环境理论 | 4篇 |
污染及防治 | 5611篇 |
评价与监测 | 1656篇 |
社会与环境 | 1453篇 |
灾害及防治 | 122篇 |
出版年
2023年 | 93篇 |
2022年 | 216篇 |
2021年 | 225篇 |
2020年 | 140篇 |
2019年 | 180篇 |
2018年 | 327篇 |
2017年 | 322篇 |
2016年 | 526篇 |
2015年 | 362篇 |
2014年 | 577篇 |
2013年 | 1748篇 |
2012年 | 674篇 |
2011年 | 859篇 |
2010年 | 802篇 |
2009年 | 785篇 |
2008年 | 887篇 |
2007年 | 977篇 |
2006年 | 870篇 |
2005年 | 716篇 |
2004年 | 725篇 |
2003年 | 704篇 |
2002年 | 670篇 |
2001年 | 912篇 |
2000年 | 631篇 |
1999年 | 395篇 |
1998年 | 274篇 |
1997年 | 246篇 |
1996年 | 291篇 |
1995年 | 270篇 |
1994年 | 250篇 |
1993年 | 233篇 |
1992年 | 240篇 |
1991年 | 208篇 |
1990年 | 214篇 |
1989年 | 219篇 |
1988年 | 197篇 |
1987年 | 158篇 |
1986年 | 126篇 |
1985年 | 138篇 |
1984年 | 169篇 |
1983年 | 153篇 |
1982年 | 193篇 |
1981年 | 134篇 |
1980年 | 119篇 |
1979年 | 152篇 |
1978年 | 119篇 |
1977年 | 107篇 |
1976年 | 100篇 |
1975年 | 83篇 |
1974年 | 90篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
351.
Gross scoliosis of the fetal thoracic spine was diagnosed at 18 weeks gestation. The pregnancy was terminated and the fetus found to have webbing of the neck and an imperforate anus in addition to vertebral defects. 相似文献
352.
M. S. Ristaldi M. Pirastu C. Rosatelli G. Monni H. Erlich R. Saiki Professor A. Cao 《黑龙江环境通报》1989,9(9):629-638
In this study, we describe a simple strategy to detect β-thalassaemia mutations in prospective parents and to make prenatal diagnosis in pregnancies at risk in the Mediterranean population. Screening of prospective parents is carried out by dot blot analysis on enzymatically amplified DNA with a set of oligonucleotide probes complementary to the most common mutations in this population. Prenatal diagnosis is accomplished by the same procedure on enzymatically amplified amniocyte or trophoblast DNA. The main advantages of this procedure are the simplicity, sensitivity (0.05 μg of DNA), and rapidity (12–24 h). Further simplification is obtained by amplification of the DNA from crude amniotic cell lysate. The very low amount of fetal material necessary for this analysis eliminates the need to culture amniotic fluid cells and may decrease the fetal loss rate associated with trophoblast sampling. The number of specific DNA sequences obtained by the amplification procedure allowed us to use non-radioactive labelled oligonucleotide probes, which have several advantages compared to radioactive probes. 相似文献
353.
Prenatal diagnosis in a kindred with the Opitz (BBB) syndrome is presented. The inheritance is consistent with either autosomal dominant inheritance with sex limited expression or X-linked inheritance. The abnormalities in the kindred consist of hypertelorism, hypospadias, ambiguous genitalia, urocolic fistula, imperforate anus, mental retardation, diaphragmatic hernia, and malrotation with volvulus. A male fetus at 19 weeks was found by ultrasound to have hypertelorism and hypospadias with a small phallus consistent with the syndrome. The diagnosis was confirmed by pathologic examination after pregnancy termination. This is the first report of prenatal diagnosis of Opitz syndrome by ultrasonographic demonstration of hypertelorism and hypospadias in the second trimester. 相似文献
354.
Amniocentesis at 17 weeks' gestation revealed a mosaic karyotype—46,XX/46,XX, — 14,+dic(14)(p11). No abnormalities were detected on ultrasound. Growth and placentation were normal. The fetus was examined after termination of pregnancy and micrognathia and pulmonary hyperlobation were the only abnormalities detected. Several tissues were set up for cytogenetics, including fetal skin, kidney, ovary, and placenta. The diagnosis was confirmed by these studies. The level of mosaicism varied between tissues, with the trisomy 14 cell line highest in amniotic fluid. 相似文献
355.
Prenatal diagnosis in a pregnancy at risk for a juvenile B1 variant of GM2-gangliosidosis was carried out. The biochemical study of the cultured amniocytes and the affected fetal brain is reported. The results obtained show that the sulphated artificial substrate can be used in the diagnosis of B1 variant, but not the neutral one. The accumulation of GM2-ganglioside in the fetal brain of the B1 juvenile form and an infantile form of GM2-gangliosidosis (0 variant) was compared. 相似文献
356.
357.
An efficient, reproducible and rapid fused silica capillary column gas chromatographic method, in splitless mode, for analysing of the three chloroanthracene isomers is described. The method affords efficient separation and good linearity for each isomer in the range of 0.2 -200 μg/ml. The method can also be used in separation and determination of another thirty three aromatic and their halosubstituted compounds. This paper also presents the applications of the method in analysing of complex products mixture from reaction of the selected aromatic compounds with hydrogen chloride on fly ash surface from municipal incinerator. 相似文献
358.
Wu Jin Wang Anpu Huang Yanchu Ma ciguang Y. Ii S. Daishim K. Furuy T. Kikuchi H. Matsushit K. Tanabe 《环境科学学报(英文版)》1990,2(1):27-39
A study has been made on elements organic constituents, TSP, SO2,NO2 of atmospheric pollutants in Beijing. 17 elements, and some PAHs, e. g. B(a)P, B(b, j, k)P, and B(g, h, i)P, in airborne particles by X-ray fluorescence spectroscopy and HPLC, GC/MS, have been determined respectively. It has been shown that the elements Pb, Zn, S and Cu were more enriched in fine particles and different valence states of sulfur at various sites. It was found that the concentrations of S6+ and S2-were more than 85% and less than 15% of the total sulfur respectively. Concentrations of major PAHs and sulfur-containing compounds increased in winter and in urban area. High values for Pb and Zn in city, Fe and Mn at industrial area and Cu, Al rural sites were obtained respectively. This implies the functions of different elemental sources of various sites. Thus, elements can be from distingushed anthropogenic and natural sources.The main contribution of SO2 was found of to have same seasonal variation as the anthropogenic el 相似文献
359.
360.
The effects of reduced oxygen concentrations in the gas phase over the culture medium on colony formation and cell proliferation were investigated in high and low cell density primary and secondary cultures of amniotic fluid cells. Using two standard culture methods (25 cm2 plastic flasks and Leighton type tubes) a significantly reduced culture time was observed at high cell density for mass cultures by incubation within a low oxygen tension gas phase (2.5 per cent to 7.5 per cent O2) instead of conventional air (18 per cent O2). At low cell density colony formation was significantly enhanced in cultures grown at reduced oxygen tension. Using gas permeable membranes as support, lowering the oxygen tension from 7.5 per cent to 2.5 per cent yielded an increase in plating efficiency of cells from approximately 5 per cent to 25 per cent, whereas plating efficiency was less than 2 per cent for cells grown at ambient 18 per cent O2. It is suggested that low oxygen tension in the gas phase is an effective means of enhancing clonal growth in amniotic fluid cell cultures, thereby reducing both culture time and risk of culture failure. 相似文献