Feasibility of weeds-based compost-cultivated Agaricus bisporus北大核心CSCD

Making full use of local weed resources to produce Agaricus bisporus is of great importance in reducing production costs and protecting the environment. In this paper, three trial experiments were conducted on the basis of weed diversity investigation around the Miyun Reservoir and the adjustment of formulation and technology in the industrial production of A. bisporus. Compost samples from different phases of the composting process and at various cultivation stages were collected for the determination of their physical-chemical properties, lignocellulose content, lignocellulolytic enzyme activities, and bacterial communities enrichment by 16S rRNA gene sequencing. The yield of mushrooms in each different trial was also calculated. The results showed several types of reservoir weeds with high, thick and hard stems. The saturated moisture of weeds was 76.78% after baling. The water content, carbon content, and C/N ratio of the samples decreased gradually during composting, but had little change during cultivation. The nitrogen content decreased at the end of phase I and increased at the end of phase II. During composting, the loss rates of hemicellulose and cellulose were both between 40% and 60%, and the loss rate of lignin was between 20% and 30%. During cultivation, instead, the loss rate of lignin was between 16% and 21%. The changes in the content of cellulose and hemicellulose of compost were consistent with that of the activity of the related degradation enzymes. A total of 432 595 valid sequences were obtained by Illumina sequencing for the samples derived from the three composting trials, and the average length of the sequences was 441 bp. Taxonomic analysis showed that the dominant bacteria were Prevotella (phylum Bacteroidetes), Bacillus (phylum Firmicutes), Thermus, Truepera, and Caldicoprobacter (phylum Deinococcus-Thermus), Thermopolyspora (phylum Actinobacteria), and Pseudoxanthomonas (phylum Proteobacteria). The yield of the three trials was in the range of 17.1-19.7 kg/m2. It is thus feasible to use reservoir weeds compost instead of wheat straw compost for the cultivation of A. bisporus. © 2018 Science Press. All rights reserved.     

Effects of exotic Spartina alterniflora on rhizosphere and endophytic bacterial community structures and diversity in roots of native mangroves北大核心CSCD

Root-associated microbial communities are very important for biogeochemical cycles of carbon, nitrogen, and sulfur in wetland ecosystems, and help to enhance the mechanisms of plant invasions. In the estuary of Jiulong River (China), Spartina alternifiora has widely invaded Kandelia obovata-dominated habitats, making it necessary to investigate the influence of rootassociated bacteria. The endophytic and rhizosphere bacterial community structures associated with selected plant species were investigated using the barcoded Illumina paired-end sequencing technique. The diversity indices of bacteria associated with the roots of S. alterniflora were higher than those of the transition stands and K. obovata monoculture. Using principal coordinate analysis with UniFrac metrics, the comparison of diversity showed that all samples could be significantly clustered into three major groups, according to the bacterial communities of origin. Four phyla, namely, Proteobacteria, Bacteroidetes, Chloroflexi, and Firmicutes, were abundant in the rhizoplane of the two salt marsh plants, whereas Cyanobacteria and Proteobacteria were the more abundant endophytic bacteria. Proteobacteria, Bacteroidetes, Chloroflexi, and Firmicutes in the rhizosphere bacteria of S. alterniflora accounted for 78.0%, 5.6%, 3.3%, and 1.6%, respectively. Members of the phyla Spirochaetes and Chloroflexi were found among the endophytic bacteria of S. alterniflora and K. obovata, respectively. Using linear discriminate analysis, we found some dominant rhizoplane and endophytic bacteria, including Pseudoalteromonadaceae, Vibrionaceae, Methylophilaceae, and Desulfovibrio, which could potentially affect the carbon, nitrogen, and sulfur cycles. Of interest was that endophytic bacteria were more sensitive to plant invasion than rhizosphere bacteria. Thus, the results provide evidence for the isolation of functional bacteria and the effects of root-associated microbial groups on S. alterniflora invasions. © 2018 Science Press. All rights reserved.     

Spatial distribution and risk assessment of soil heavy metal contamination along roadsides of the northwestern Sichuan Plateau,China北大核心CSCD

Traffic activities in roadways are the major source of heavy metal contamination on the northwestern Sichuan Plateau, China. To characterize the spatial pattern of heavy metal distribution, we collected soil samples and measured the concentrations of five heavy metals (Cd, Cu, Ni, Pb, and Zn) from nine sites in Hongyuan County, Sichuan Province, including three transects that reflected typical gradients of traffic density. Each transect consisted of three levels of traffic density sites. We calculated single pollution index, Nemerow multi-factor index, and potential ecological risk index, to assess the ecological risk of the heavy metal contamination. Results showed that the soils were contaminated by Cd and Zn, with higher concentrations than that of the natural soil background values in all sampling sites. Moreover, Cd and Zn concentrations increased with increasing traffic density, suggesting that traffic activities were potentially responsible for the metal contaminations. The single pollution index indicated that the study sites were heavily contaminated by Cd and slightly polluted by Ni, Pb, and Zn, and the Nemerow multi-factor and potential ecological risk indexes indicated moderate potential ecological risks at the study sites. Specifically, the sites with high traffic density were moderately contaminated and the sites with low traffic density were mildly contaminated. In general, Cd and Zn were highly accumulated in soils of the study region; therefore focus should be on the high ecological hazard associated with soil heavy metal contamination, even in undeveloped regions. © 2018 Science Press. All rights reserved.     

Isolation,identification, and the degradation characteristics of a BDE-47 degrading strain北大核心CSCD

In an effort to remove BDE-47 residues from the environment, a bacterial strain that is capable of utilizing BDE-47 as the sole carbon source was isolated and screened from soil collected from an e-waste recycling area in Tianjin to analyze the degradation properties. The strain was preliminarily identified as Enterobacter sp. according to a 16S rDNA gene sequence analysis. The strain degraded 35.8% of 525 μg/L of BDE-47 in 35 d when the initial concentration of bacteria was 7.1 × 105 cells/ mL. The product of the biodegradation of BDE-47 was BDE-28. The biodegradation of BDE-47 fit well with first-order kinetics, and its degradation kinetics was ln Ct = - 0.104t + 6.22. With the addition of an electron acceptor, such as Fe3+, SO4 2- and NO3 -, the BDE-47 degradation rate was significantly increased to 49.8%, 59.1%, and 67.3%, respectively. The above results revealed that the strain could degrade BDE-47, which is of importance in the application of environmental bioremediation of BDE-47. © 2018 Science Press. All rights reserved.     

Volatile components and their corresponding synthetic gene expression profile in the fruits of Actinidia chinensis北大核心CSCD

This study aimed to identify the difference in volatile aromatic components and the relationship with the expression pattern of their corresponding bio-synthesis genes in six kiwifruit (Actinidia chinensis) varieties (Cuiyu, Jintao, Jinyan, Chuhong, Donghong, and Xixuan). To provide a foundation for kiwifruit variety recognition, fruit quality evaluation, and molecular-assisted breeding, the volatile aromatic components in the ripe fruits of six kiwifruit varieties were evaluated by head space-solid phase micro extraction/gas chromatography-mass spectrometry. The aroma-synthesis-related genes, including acyltransferases (AcAT16), lipoxygenase (AcLox2), and terpene synthase genes (AcTPS1), were detected by the real time-quantitative polymerase chain reaction (qPCR) during the postharvest stage of fruits. Ninety-two aroma chemicals were identified in the tested kiwifruit cultivars. There were 35, 32, 30, 44, 28, and 17 of aromatic compounds in Cuiyu, Jintao, Jinyan, Chuhong, Donghong, and Xixuan, respectively. Esters were the main aroma components in Cuiyu, Jintao, and Jinyan. The major aromatic compounds of Chuhong, Donghong, and Xixuan were aldehydes and terpenoids. The expression level of AcATs16 and AcLox2 increased, and then decreased during the ripening of kiwifruit fruits. The expression of AcATs16 was significantly higher in Cuiyu and Jinyan than in other varieties. AcLox2 indicated significant abundance in Cuiyu and Chuhong. AcTPS1 was up-regulated in Jintao, Donghong, and Xixuan with fruit ripening; however, this was not observed in Cuiyu, Chuhong, and Jinyan. The difference in the composition and content of volatile aromatic components contributes to the difference in aroma in different kiwifruit cultivars. The diverse expression of AcATs16, AcLox2, and AcTPS1 might be closely related to the synthesis of ethyl butyrate, (E)-2-hexenal, and eucalyptol, respectively. © 2018 Science Press. All rights reserved.     

Duckweed Landoltia punctata purifies micro-polluted surface water and produces starch北大核心CSCD

Aquatic plant duckweed has remarkable potential in nutritional water purification and starch accumulation; at present, it has received increasing attention. This study aimed to investigate the ability of duckweed in nutrient recovery from micro-polluted surface water; further, the starch accumulation capacity of duckweed was evaluated. The results showed that duckweed can achieve better depth treatment of the micro-polluted surface water, within 1-day treatment, by duckweed. Ammonia nitrogen and total phosphorus status of Class V and worse than class V water was improved to a superior level; moreover, the nitrogen and phosphorus removal rates were 98.5% and 82.9%, respectively. In addition, duckweed can rapidly accumulate starch during water treatment. The starch content of duckweed was 28.38% and 21.57% (dry weight) in Class V and worse than class V wastewater after 3 days of treatment, respectively, and reached 52.15% and 49.58% on day 15. Moreover, additional carbon dioxide (CO2) supplementation promoted the starch production. The starch content increased by 55.7% compared with that of control, and the average starch accumulation rate increased by 2.72 times in 3 days. Therefore, duckweed can not only rapidly purify micro-polluted water, but also accumulate a large amount of starch. This study forms the basis for wastewater treatment and post-treatment utilization of duckweed biomass. © 2018 Science Press. All rights reserved.     

Mechanism of limestone corrosion by Gongronella butleri NL-15北大核心CSCD

The key to soil spray-sowing technology is the use of the highly-effective rock-corroding strains, and the mechanism of rock corrosion will provide theoretical references to screen the strains. To investigate the corrosion mechanism of limestone by microorganisms, the dominant and highly-effective limestone-corroding strain Gongronella butleri NL-15 was isolated and purified from the microorganisms on the rock surface. The concentration of calcium (Ca), magnesium (Mg), potassium, and phosphorus was analyzed by ICP. The pH of the fermentation broth of the strain at different intervals was measured using a precise pH instrument. The content of organic acids (critic, succinic, lactic, fumaric, acetic, and propanoic acids) and the scanning electron microscope feature of rock particles in the fermentation broth at different intervals were also analyzed by HPLC. The results showed that the concentration of Ca2+ and Mg2+ in the fermentation broth correlated negatively with the pH value (P < 0.01). The pH of control was 6.87, and that of the fermentation broth after 15 days dropped to 5.12. The concentration of Ca and Mg was 38.96 and 10.85 mg/L respectively, whereas that of the fermentation broth after 15 days increased to 367.56 and 76.16 mg/L respectively. The content of the total organic acids and lactic acid increased with increase in fermentation time. The content of the total organic acids on days 2, 5, 9, and 15 in the fermentation broth was 3.43, 5.40, 6.63, and 7.26 mg/mL, respectively, and the content of lactic acid was 1.79, 2.85, 5.16, and 5.04 mg/mL, respectively. The mycelium of the G. butleri NL-15 adhered to the surface of rock particles and threaded into unconsolidated rocks. Thus, the organic acids, especially lactic acid produced by the fungi G. butleri NL-15, caused unconsolidation of rock and improved the growth of fungi mycelium into the unconsolidated rock. This was the primary cause for limestone corrosion, and the release of Ca and Mg from the rock and its disintegration. © 2018 Science Press. All rights reserved.     

Assessment of heavy metal pollution and health risks in a main river in Huayuan County,Xiangxi, Hunan Province北大核心CSCD

To study heavy metal pollution and assess the health risk of river water in Huayuan County, Xiangxi, Hunan Province, 11 water samples were collected from the Huayuan River and Brother Rivers in August and December 2016. Heavy metal (Pb, Zn, Cr, Cu, Fe, and Ni) concentrations were determined from the samples. The health risk assessment model recommended by the U.S. Environmental Protection Agency (USEPA) was applied to assess the health risk of heavy metals in the main surface waters of Huayuan County. The results indicated that the concentrations of heavy metals (Pb, Zn, Cr, Cu, Fe, and Ni) of surface water in the research area were 2.57 × 10-3, 4.66 × 10-4, 1.65 × 10-3, 6.27 × 10-4, 0.19, and 8.50 × 10-4 mg/L, respectively. The health risk of surface waters with heavy metals was high. Therefore, the chemical carcinogenic substance (Cr) health risk index was five or six times higher than that of chemical non-carcinogens (Pb, Zn, Cu, and Ni). The average health risk indices of non-carcinogenic substances were in the order Pb > Cu > Zn > Ni. The correlation and principal component analysis of surface water showed that the six heavy metal elements were composed of three main components in the main surface waters of the county. The first principal component was comprised of Fe and Ni (33.28%), which was mainly from internal pollution. The second component was comprised of Cu and Cr (26.98%), which was primarily due to industrial waste water, rainwater leaching mineral waste produced by heavy metal mining, and smelting enterprises. The third component, resulting from geochemical pollution, was Zn (17.10%). The health risk indices triggered by heavy metal in surface waters was high. Heavy metal pollutants in the research area need to be controlled in the order Cr, Pb, Cu, Zn and Ni. © 2018 Science Press. All rights reserved.     

Field tests to study the dynamics of weed inhibition of allelopathic rice and microbial physiological traits of rice rhizospheric soils北大核心CSCD

The weed inhibition of allelopathic rice PI312777 and nonallelopathic rice Lemont, allelopathic potential of rice rhizospheric soils, as well as the microbial physiological traits of rice rhizospheric soils, were studied by field tests after weedremoving and weed treatments. The results showed that the inhibitory rate of PI312777 at the 7-leaf stage on paddy weeds was 85.82%. Results of the Soil-Agar Sandwich method revealed that the allelopathic potential of PI312777 rhizospheric soils on the inhibitory rate of plant dry weight of barnyard grass was significantly higher at the 5-leaf stage than that at the 3-leaf stage, and increased by 20.16% from the 3-leaf stage to the 5-leaf stage after weed treatment. When at the same leaf stage, the soil microbe biomass carbon and soil respiration, the number of soil bacteria, and activity of soil enzymes (urease, protease, and sucrase) were significantly higher in PI312777 rhizospheric soils than in Lemont rhizospheric soils; they were also higher after the weed-removing treatment than after weed treatment. The largest increase of soil allelopathic potentials and soil microbial physiological indexes in PI312777 rhizospheric soils appeared from the 3-leaf stage to the 5-leaf stage. In case of weed treatment, the allelopathic potential of PI312777 rhizospheric soils on the soil microbe biomass carbon, soil respiration, the number of soil bacteria, activity of urease, activity of protease, and activity of sucrase increased by 53.11%, 51.56%, 38.97%, 44.83%, 60.00%, and 41.92%, respectively, from the 3-leaf stage to the 5-leaf stage. These results indicated that rice allelopathy had a close relationship with the activity of rhizospheric soils. Rice allelochemicals lead to the change of soil microbes; rice allelopathy is a process based on plant-soil interaction. © 2018 Science Press. All rights reserved.     

Bulk deposition of polycyclic aromatic hydrocarbons (PAHs) in an industrial site of Turkey

Ambient air and deposition samples were collected in the period of July 2004-May 2005 in an industrial district of Bursa, Turkey and analyzed for polycyclic aromatic hydrocarbon (PAH) compounds. The overall average of fourteen bulk deposition fluxes for PAHs was 3300+/-5100 ng m(-2) d(-1). PAH depositions showed a seasonal variation and they were higher in winter months. This was probably due to increases in residential heating activities and decreases in atmospheric mixing layer levels. Ambient air samples, measured with a high volume air sampler, were collected from the same site. The average total concentration including gas and particulate phase was about 300+/-420 ng m(-3) and it was in the range of previously reported values. Some of the ambient air and bulk deposition samples were collected simultaneously in dry periods. Both concurrently measured values were used to calculate the dry deposition velocities whose overall average value was 0.45+/-0.35 cm s(-1).