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Molecular techniques and modelling are presented as powerful tools required in the performance of efficient soil and water bioremediation systems. An Escherichia coli CC118-D strain was constructed by inserting the Klebsiella pneumoniae hpa B gene, coding for the unstable 3,4-dihydroxyphenylacetate 2,3-dioxygenase, into its chromosome. When the constructed strain was immobilized, both enzyme stability and viability increased along the studied period, in absence of antibiotic. We proposed this strategy as an approach to overcoming plasmid instability and to enhance enzyme activity and stability, avoiding antibiotic utilization. A model was developed to understand and predict the behaviour of bacteria and pollutants in a bioreactor system, considering: fluid dynamics, molecular/cellular scale processes and biofilm formation.  相似文献   
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The project ENMA assesses the atmospheric pollution caused by the transformation and consumption of energy. The project is in the following stages: (a) estimation of annual overall average emissions; (b) estimation of annual average concentrations of pollutants; and (c) estimation of doses. Estimation of annual overall average emissions is estimated, separately, for urban areas and point sources. Concentrations of pollutants are obtained for urban areas and point-sources by means of two different gaussian models. Finally, doses are calculated assuming that the effects of all pollutants are linear at low doses. Doses for each pollutant, and doses for exposure to several combined pollutants, are defined. The doses on population are also defined, assuming that the significance of pollution depends on the number of people exposed.  相似文献   
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The historic development of the scientific interest in monitoring CO2 in the atmosphere is the subject of this article. Particular emphasis is placed upon activities initiated by the USA in the 1950s which led to the establishment of the Mauna Loa Observatory and further developed into the existing world-wide monitoring system for air constituents and air pollution operated under the auspices of the World Meteorological Organization, a major contributer to the Global Environmental Monitoring System of the UN Environment Programme. Recent studies on the feasibility of monitoring the background level of CO2 at stations throughout the world have indicated considerable difficulties resulting from the influences of the biosphere. These problems have led WMO to adopt new criteria for CO2 monitoring station locations which are presented in a discussion of future needs and plans for global monitoring of CO2 in the atmosphere.  相似文献   
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Summary This paper looks at the problems of science and technology education in Mexico, and proposes ways in which the quality of education at every level can be improved. It advocates an interdisciplinary approach, and from that approach to science education, the development of environmental education.Prof. Gutiérrez-Vázquez received his training in microbiology and biochemistry in the National Polytechnic Institute in his native Mexico City, receiving his degree in 1954. He went from instructor to assistant professor (1950–1960), becoming a full professor, deputy dean and then dean of the Faculty of Biology in the same Institute (1961–1969); he published two books and some 20 papers dealing with the physiology of tubercle bacillus and other mycobacteria, as well as with other topics on microbiology. More recently (1970 to date), he became a full professor in the Center for Advanced Studies of the same Institute, where he has been head of the Department of Educational Research (1972–1981) and carried out research and development projects on science education for children and adolescents. During this period he has published some 20 more books and 30 odd papers, and written more than 80 science programmes for commercial television.  相似文献   
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In the present work, Acid Mine Drainage (AMD) processes in the Chorrito Stream, which flows into the Cobica River (Iberian Pyrite Belt, Southwest Spain) are characterized by means of clustering techniques based on fuzzy logic. Also, pH behavior in contrast to precipitation is clearly explained, proving that the influence of rainfall inputs on the acidity and, as a result, on the metal load of a riverbed undergoing AMD processes highly depends on the moment when it occurs. In general, the riverbed dynamic behavior is the response to the sum of instant stimuli produced by isolated rainfall, the seasonal memory depending on the moment of the target hydrological year and, finally, the own inertia of the river basin, as a result of an accumulation process caused by age-long mining activity.  相似文献   
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