Scenario‐Based Evaluation of Commercially Available Cleaning Robots for Collection of Bacillus Spores from Environmental Surfaces

The cleaning robots, a vacuum‐based robot (R2) and a wetted‐wipe‐based robot (R4), were evaluated in this study to determine their effectiveness for sampling Bacillus atrophaeus spores. The tests were designed to evaluate the benefit of robot sampling on large areas with two different contamination scenarios: a high‐concentration, low spatial extent contamination (hot spot) and a low concentration, high spatial extent contamination (widely dispersed). The hot spot tests were conducted with the high spore contamination (approximately 10⁷ colony forming units [CFUs]) on a limited area (30.5 cm × 30.5 cm), 2 percent of the entire test. The widely dispersed tests were conducted with approximately 0.1 CFUs/cm² for floor laminate and approximately 10 CFUs/cm² for carpet surfaces. The widely dispersed tests distributed spores across the test surface and covered approximately 40 percent of the entire test area. The test results showed that both robots successfully sampled a large quantity of spores from carpet and floor laminate surfaces for both test scenarios. Robot performance is discussed in the context of currently used surface sampling methods. © 2014 Wiley Periodicals, Inc.*     

Root respiration and oxygen flux in salt marsh grasses from different elevational zones

Plants growing in waterlogged environments are subjected to low oxygen levels around submerged tissues. While internal oxygen transport has been postulated as an important factor governing flooding tolerance, respiration rates and abilities to take up oxygen under hypoxic conditions have been largely ignored in plant studies. In this study, physiological characteristics related to internal oxygen transport, respiration, and oxygen affinity were studied in low intertidal marsh species (Spartina alterniflora and S. anglica) and middle to high intertidal species (S. densiflora, S. patens, S. foliosa, a S. alterniflora × S. foliosa hybrid, S. spartinae, and Distichlis spicata). These marsh plants were compared to the inland species S. pectinata and the crop species rice (Oryza sativa), corn (Zea mays), and oat (Avena sativa). Plants were grown in a greenhouse under simulated estuarine conditions. The low marsh species S. anglica was found to transport oxygen internally at rates up to 2.2 μmol O₂ g fresh root weight⁻¹ h⁻¹. In contrast, marsh species from higher zones and crop species were found to transport significantly less oxygen internally, although rice plants were able to transport 1.4 μmol g⁻¹ h⁻¹. Under hypoxic conditions, low marsh species were better able to remove dissolved oxygen from the medium compared to higher marsh species and crops. The oxygen concentration at which respiration rates declined due to limited oxygen (P _crit) was significantly lower in low marsh species compared to inland and crop species; P _crit ranged from <4 μM O₂ in the low marsh species S. anglica up to 20 μM in the inland species corn. Flooding-sensitive crop species had significantly higher aerobic respiration rates compared to flooding-tolerant species in this study. Crop species took up 3.6–6.7 μmol O₂ g⁻¹ h⁻¹ while all but one marsh species took up <3.5 μmol O₂ g⁻¹ h⁻¹. We conclude that oxygen transport, aerobic demand, and oxygen affinity all play important and interrelated roles in flood tolerance and salt marsh zonation.     

Partitioning coefficients of polycyclic aromatic hydrocarbons in stack gas from a municipal incinerator

In this study, solid-gas partitioning coefficients of PAHs on fly ash in stack gas from a municipal incinerator were determined according to elution analysis with gas-solid chromatography. The fly ash from the electrostatic precipitator was sieved and packed into a 1/4 inch (6.3 mm) pyrex column. Elution analysis with gas-solid chromatography was conducted for three PAHs, Napthalene, Anthracene, and Pyrene. The temperature for elution analysis was in the range of 100°C to 300°C. V_g, specific retention volume obtained from elution analysis, and S, specific surface area of fly ash measured by a surface area measurement instrument, were used to estimate the solid-gas partitioning coefficient, K_R. In addition, the relationships between K_R and temperature and K_R and PAH concentrations were investigated.     

EVALUATION OF GREAT LAKES NET BASIN SUPPLY FORECASTS1

ABSTRACT: Evaluation of the Great Lakes Environmental Research Laboratory's (GLERL's) physically-based monthly net basin supply forecast method reveals component errors and the effects of model improvements for use on the Laurentian Great Lakes. While designed for probabilistic outlooks, it is assessed for giving deterministic outlooks along with other net basin supply forecast methods of the U.S. Army Corps of Engineers and Environment Canada, and with a stochastic approach commissioned by the Corps. The methods are compared to a simple clima-tological forecast and to actual time series of net basin supplies. Aetual net basin supplies are currently determined by estimating all components directly, instead of as water-balance residuals. This is judged more accurate and appropriate for both forecasting and simulation. GLERL's physically-based method forecasts component supplies while the other methods are based on residual supplies. These other methods should be rederived to be based on component supplies. For each of these other methods, differences between their outlooks and residual supplies are used as error estimates for the rederived methods and component supplies. The evaluations are made over a recent period of record high levels followed by a record drought. Net basin supply outlooks are better than climatology, and GLERL's physically-based method performs best with regard to either component or residual net basin supplies. Until advances are made in long-range climate outlooks, deterministic supply outlooks cannot be improved significantly.     

Water quality issues in the Nakdong River Basin in the Republic of Korea

A framework for evaluating alternative management strategies for the Nakdong River Basin in the Republic of Korea (South Korea) was developed and applied jointly by Argonne National Laboratory in Argonne, Illinois, USA, and the Research Institute of Industrial Science and Technology in Pohang, South Korea. Water from this basin, the second largest in South Korea, supports a total population of more than 13 million people. Rapid industrial expansion, urbanization, and population growth have dramatically increased the demand for water and have severely degraded water quality, particularly near large industrial complexes and in the lower portion of the basin. Management strategies for the entire basin through to the year {dy2011} were evaluated with a computer model for basin-wide predictions of water flow and quality (HSPF). This continuous-event model was developed and calibrated using site-specific data for the basin over a two-year period (1994-1995) that included periods of both high (monsoonal) and low (drought) flows. Water quality impacts for different wastewater treatment strategies were assessed in terms of biochemical oxygen demand (BOD), nitrogen (N) and phosphorous (P) levels. The results of the study indicate that BOD levels in the main stem of the Nakdong River below the Kumhogang (a major tributary with low flow and heavy pollution loads) cannot be significantly improved by reducing direct BOD loads from point sources along the river. To reduce main stem BOD levels, the nutrient loading to the river must be reduced. In order to reduce these loads, additional advanced treatment methods (i.e., tertiary treatment) must be incorporated in the treatment facilities to remove N and P. The discharge inventory data further suggest that a large portion of N and P loads are derived from non-point agricultural practices. Reduction of these loads is difficult to accomplish and may require extensive modifications in agricultural and land-use practices. The modeling frame-work developed provides a means to evaluate these and other basin management strategies.