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101.
'Molecular farming' is the production of valuable recombinant proteins in transgenic organisms on an agricultural scale. While plants have long been used as a source of medicinal compounds, molecular farming represents a novel source of molecular medicines, such as plasma proteins, enzymes, growth factors, vaccines and recombinant antibodies, whose medical benefits are understood at a molecular level. Until recently, the broad use of molecular medicines was limited because of the difficulty in producing these proteins outside animals or animal cell culture. The application of molecular biology and plant biotechnology in the 1990s showed that many molecular medicines or vaccines could be synthesised in plants and this technology is termed 'molecular farming'. It results in pharmaceuticals that are safer, easier to produce and less expensive than those produced in animals or microbial culture. An advantage of molecular farming lies in the ability to perform protein production on a massive scale using hectares of cultivated plants. These plants can then be harvested and transported using the agricultural infrastructure. Thus, molecular farming allows rapid progress from genetic engineering to crop production, and new cash crops producing recombinant proteins are already being commercially exploited. We speculate that as functional genomics teaches us more about the nature of disease, molecular farming will produce many of the protein therapeutics that can remedy it.  相似文献   
102.

The intensive investigation site ‘Mühleggerköpfl’ in the North Tyrolean Limestone Alps can be classified as a clean-air area site. The mean concentrations of NOx are far below the effect-related limit value of the WHO (30 μg NOx m−3). The gravitational depositions in the open field (bulk deposition) ranged from 10.8 to 14.7 kg N ha−1 a−1 (throughfall: 11.3 to 12.3 kg N ha−1 a−1) in the measuring years 1998 to 2000. Compared to these data, depositions in other forested areas of the Austrian Alps amounted to up to 30 kg N ha−1 a−1. The gravitational depositions (bulk deposition) alone - without considering dry and occult deposition - slightly exceeded the lower limit of Critical Loads for coniferous and deciduous forests (> 10 kg N ha−1 a−1), but were below the Critical Loads for calcareous forests (15–20 kg N ha−1 a−1).

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This 3-year study was designed to examine variation in plasma sex steroids, phallus size, and the standard error (S.E.) associated with these endpoints in juvenile alligators collected from 3 sites within the Kissimmee-Everglades drainage (Florida, USA) with varying concentrations of sediment organochlorine contaminants. We hypothesized that decreased plasma sex steroid concentrations and phallus size would be observed in the higher contaminant site when compared to the intermediate and lower contaminant sites. Furthermore, we hypothesized that greater S.E. associated with these endpoints would be observed for the populations from more contaminated sites. We found that differences existed with females from the higher contaminant site exhibiting lower plasma estradiol-17beta (E2) and testosterone (T) concentrations. Males from the higher contaminant site exhibited smaller phallus sizes than males from the intermediate and lower contaminant sites. Smaller phallus size in this case differed from that reported in Lake Apopka male alligators [Gen. Comp. Endocrinol. 116 (1999) 356] in that a significant positive relationship between body size and phallus size existed. No difference among sites was observed in plasma T for males. Lower S.E. was associated with E2 and T concentrations in females from the higher contaminant site and in phallus size in males from the higher contaminant site. This pattern was opposite to what we had hypothesized. We concluded that variation in plasma E2 and T concentrations, phallus size, and the S.E. associated with these endpoints exists among the 3 sites with the patterns matching the patterns of organochlorine contamination, although S.E. patterns were opposite to what was predicted.  相似文献   
105.
This paper examines the legitimacy, viability and efficiency of the European Union Solidarity Fund by asking whether the Fund meets its stated purpose of providing solidarity within the EU, whether it is sufficiently capitalized and if it promotes disaster risk reduction in Europe. In examining these questions, we make use of ADAM models of disaster risks throughout Europe. We conclude that the Solidarity Fund falls short on all three counts, and we suggest possible alternatives. Most far-reaching, we explore whether the EUSF could support insurance systems in Europe by, among other possible activities, capitalizing national public-private insurance programs and providing support for government risk transfer. This would leverage the Fund’s capital and would overcome barriers to the provision of private and public sector insurance in uncertain catastrophe markets. It would also make insurance more affordable to Europe’s most vulnerable communities. Finally, it would reduce the disincentives for risk reduction inherent in post-disaster assistance.  相似文献   
106.
ABSTRACT: Most spatial decision support systems for natural resource planning and management are limited by their scenario-based (non-behavioral), deterministic (non-stochastic) structure. A spatial decision support system is developed that uses a multiple attribute decision-making model to explain how a property manager selects a land and water resource management system (LWRMS) based on its multiple, stochastic economic and environmental attributes. The decision support system assesses sustainable resource management at the property and watershed scales and identifies the most cost-effective policy for enhancing sustainable resource management. Economic attributes are determined with an economic model and environmental attributes are simulated with an environmental model. Input parameters for both models are generated with a geographic information system. The decision support system is used to rank five LWRMS for a sample of 20 farmers in Missouri's Goodwater Creek watershed and for two hypothetical watershed alliance groups. Results indicate that the average farmer and the two alliance groups would rank the five LWRMS in the same manner. From the viewpoint of the watershed alliance, the most preferred LWRMS for the average farmer in the watershed is sustainable.  相似文献   
107.
We investigated migration and wintering of adult northern gannets (Morus bassanus) breeding in east Scotland, North Sea, by deploying geolocation loggers over three winters. The wintering ranges of these birds varied from the North Sea to the Atlantic off West Africa. Flight time was taken as a proxy for migration and foraging effort. Gannets wintering off Africa had higher total flight times during migration than birds wintering further north. Total flight times in different wintering regions were generally low. Birds off West Africa consistently spent < 25 % of daylight hours in flight, but birds further north showed more variable values that may reflect more variable weather or food availability. Winter sea surface temperatures ranged from 9 °C (North Sea) to 16 °C (West Africa). Thermostatic costs in winter as estimated by measuring thermal conductance in carcasses in still air and water were 28 % higher in North Sea than off West Africa. This effect is aggravated by higher thermostatic costs caused by stronger wind chills in the North Sea compared to the conditions off West Africa. Birds wintering close to the UK arrived at the colony on average 12 days earlier than birds wintering off West Africa. We conclude that the net cost-benefit analysis may be similar for all wintering areas investigated.  相似文献   
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109.
In mammals, the chemical profiles of individuals are complex and variable mixtures, and animals perceive information based on variation in the overall quality of these mixtures. A variety of compounds potentially involved in chemical communication have been characterized in the urine of different felid species, but little is known about the information content of felid scent marks. In this study, we investigated whether chemical composition of Eurasian lynx Lynx lynx urine was related to sex, reproductive state, and individual identity. We further analysed if elemental sulphur in lynx urine could serve as a dietary cue or as an indicator for the freshness of a scent mark. We collected urine from captive and wild Eurasian lynx, and analysed volatile constituents of urine by means of solid phase microextraction and gas chromatography-mass spectrometry. Our results show that lynx scent profiles contain sex-specific information on reproductive state, as well as individual identity cues. Urine marks are, therefore, well-suited to fulfil a role in reproductive behaviour and social organisation of wild lynx populations. Relative sulphur content was unrelated to time since last feeding, but decreased with age of the urine sample. The influence of diet and body condition on scent profiles should be further investigated by means of experimental studies, and may shed more light on the messages encoded in carnivore scent-marks.  相似文献   
110.
The blowfly Lucilia bufonivora shows high host specificity for toads despite the host’s toxic skin secretion, which consists mainly of bufadienolides. These toxins are effective blockers of the Na+, K+-ATPase, an enzyme that is essential for many physiological processes in animals. Whereas common toad (Bufo bufo) toxins were identified in the larvae of the fly, few toxins were found in the pupae and empty puparia as trace amounts, while adult flies were entirely free of these toxic compounds. Similar results were obtained when larvae of generalist necrophagous blowflies (L. sericata, Calliphora vicina) fed on toad carcasses. Analysis of the Na+, K+-ATPase gene revealed no amino acid substitution at positions known to mediate resistance to bufadienolides in other systems. Alternative mechanisms of resistance such as efficient excretion of the compounds may enable the flies to use this poisonous food source.  相似文献   
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