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11.
Silver nanoparticle antimicrobial activity explained by membrane rupture and reactive oxygen generation 总被引:1,自引:0,他引:1
Silver nanoparticles are widely used as antimicrobial compounds based on empirical observations. However, there is few knowledge on the mechanism ruling the antimicrobial activity and toxicity of Ag nanoparticles. Here, we investigated this mechanism. Nano-Ag was synthesised by thermal co-reduction. Mutagenicity analysis was performed using Salmonella typhimurium histidine auxotrophic strains TA 98 and TA 100 at nano-silver concentrations of 100 to 500 µg per plate. Dose-dependent analysis for reactive oxygen species generation has been performed using 2,7-dichlorofluorescein diacetate dye. Membrane integrity has been analyzed at 260 nm, before and after treatment. We also used scanning electron microscopy, membrane permeabilization test, and superoxide formation determinations. Results show that the average particle size of Ag nanoparticle is 60.4 ± 3.8 nm. The minimum inhibitory concentration of Ag nanoparticles for E.coli is 30 µg/mL; the minimum bactericidal concentration is 40 µg/mL. Ames mutagenicity tests showed negative results, which may be explained by the antimicrobial activity of nano-silver. Bacterial inner wall were indeed ruptured, and cytoplasmic content was released after 5 min of treatment in a dose-dependent manner. We thus propose that reactive oxygen generation and alteration of membrane integrity and permeability are the major mechanism of antimicrobial activity of nano-silver. 相似文献
12.
应对不确定性:呼吁建立新的科学政策论坛 总被引:4,自引:1,他引:4
Ann Kinzig David starrett Kenneth Arrow Sara Aniyar Bert Bolin Partha Dasgupta Paul Ehrlich Carl Folke Michael Hanemann Geoff Heal Michael Hoel AnnMari Jansson Bengt-Owe Jansson Nils Kautsky Simon Levin Jane Lubchenco Karl-Gran Miler Stephen W.Pacala Stephen H.Schneider Domenico Siniscalco Brian Walker 《Ambio-人类环境杂志》2003,32(5):330-335
科学界和政策界具有不同的目标,这就自然导致了对构成某种变化或现象的"检验"标准的差异,以及用不同的方法去描述不确定性和风险的持性及传达不确定性和风险.这些差异可能损害科学家、决策者和公众之间的有效沟通,同时也限制了科学家所愿意研究的社会紧迫问题的类型.本文主要回顾了一系列处理不确定性问题的方法,阐述了当科学和政策不能很好协调时所可能引起的一些错误.并在此基础上,特别针对解决不确定性问题提出了一些具体建议,包括重构科学规范,建立由科学、政策两方面领导广泛参加的科学政策论坛. 相似文献
13.
Kinzig A Starrett D Arrow K Aniyar S Bolin B Dasgupta P Ehrlich P Folke C Hanemann M Heal G Hoel M Jansson A Jansson BO Kautsky N Levin S Lubchenco J Mäler KG Pacala SW Schneider SH Siniscalco D Walker B 《Ambio》2003,32(5):330-335
The scientific and policy worlds have different goals, which can lead to different standards for what constitutes "proof" of a change or phenomena, and different approaches for characterizing and conveying uncertainty and risk. These differences can compromise effective communication among scientists, policymakers, and the public, and constrain the types of socially compelling questions scientists are willing to address. In this paper, we review a set of approaches for dealing with uncertainty, and illustrate some of the errors that arise when science and policy fail to coordinate correctly. We offer a set of recommendations, including restructuring of science curricula and establishment of science-policy forums populated by leaders in both arenas, and specifically constituted to address problems of uncertainty. 相似文献
14.
Walia Niharika Dasgupta Nandita Ranjan Shivendu Ramalingam Chidambaram Gandhi Mansi 《Environmental Chemistry Letters》2019,17(2):991-1002
Environmental Chemistry Letters - Vitamin deficiency arises when the dietary intake of essential vitamins is too low. Insufficient levels of vitamin weaken the body and induce diseases. Although... 相似文献
15.
This paper investigates the effectiveness of protected areas in slowing tropical forest clearing in 64 countries in Asia/Pacific, Africa and Latin America during the period of 2001–2012 by comparing deforestation rates inside and within 10 km outside of the boundaries of protected areas. Annual time series of these deforestation rates were constructed from recently published high‐resolution data on forest clearing from Hansen et al. (2013). For 4,028 parks, panel estimation based on a variety of park characteristics was conducted to test if deforestation was lower in protected areas because of their protected status, or if other factors explained the difference. From a sample of 726 parks established since 2002, a test was also conducted to investigate the effect of park establishment on protection. Findings suggest park size, national park status and management by indigenous people all are significantly associated with effective protection across regions. For the Asia/Pacific region, the test offers compelling evidence that park establishment has a near‐immediate and powerful effect. 相似文献
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17.
Poddar Nanda Das Susmita Dhar Subham Mondal Kajal Kumar 《Environmental Fluid Mechanics》2021,21(5):1069-1090
Environmental Fluid Mechanics - Predicting the evolution of environmental dispersion of settling particles in wetland flows has a wide range of applications in ecological engineering. In the... 相似文献
18.
Dasgupta R Chakravorty PP Kaviraj A 《Journal of environmental science and health. Part. B》2012,47(2):99-103
Effects of sub-lethal doses of carbaryl (1-Naphthyl-methylcarbamate), chlorpyrifos (O,O-diethyl O-3,5,6-trichloro-2-pyridinyl-phosphorothioate) and endosulfan (6,7,8,9,10,10-Hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepin-3-oxide), respectively a carbamate, an organophosphate and an organochlorine insecticide on growth, reproduction and respiration of the tropical earthworm, Perionyx excavatus (Perrier) were investigated under laboratory conditions. The results showed significant reduction in biomass, production and hatching of cocoon and production of juveniles of the worms exposed to 0.75 to 3.03 mg/kg soil of carbaryl, 0.91 to 3.65 mg/kg soil of chlorpyrifos and 3.75 to 15.0 μg/kg soil of endosulfan corresponding to 12.5 to 50 % of LC(50) value of the respective insecticide for P. excavatus. Endosulfan was found most dangerous among the three insecticides followed by carbaryl and chlorpyrifos. There was no hatching of the worms at endosulfan treatment 5.0 μg/kg soil (25 % LC(50)) or above while the highest dose of carbaryl and chlorpyrifos (50 % of LC(50)) rendered respectively 87.13 and 24.84 % reductions in hatching as compared to control. Chlorpyrifos produced no change in respiration of the worms except at the highest dose, while the worms showed an increase in evolution of CO(2) at all doses of carbaryl and endosulfan. Based on the recommended agricultural dose of each insecticide, it was concluded that application of endosulfan and carbaryl was potentially dangerous to earthworms. 相似文献
19.
Genotoxicity of oxy-PAHs to Japanese medaka (Oryzias latipes) embryos assessed using the comet assay
Subham Dasgupta Austin Cao Brittany Mauer Beizhan Yan Seiichi Uno Anne McElroy 《Environmental science and pollution research international》2014,21(24):13867-13876
Polycyclic aromatic hydrocarbons (PAHs) have long been recognized as important environmental toxicants. Despite a plethora of information on the fate and effects of parent PAHs, relatively little is known about the environmental fate and toxicity of ketone- and quinone-substituted PAH oxidation products (termed oxy-PAHs), particularly in the aquatic environment. This study begins to fill that gap using embryos of the Japanese medaka (Oryzias latipes) as a model species. The genotoxic potential of two environmentally relevant oxy-PAHs, acenaphthenequinone and 7,12-benz[a]anthracenquinone, was assessed using the comet assay. We found that both oxy-PAHs could cause significant increases in DNA damage after only 48 h of exposure at the lowest concentrations tested (5 μg/L). Comparisons of the genotoxic potential between these oxy-PAHs and their corresponding parent PAHs (acenaphthene and benz[a]anthracene) and a well-known mutagenic PAH, benzo[a]pyrene, indicated similar potencies among all five of these compounds, particularly after longer (7 day) exposures. This study demonstrates the mutagenic potential of oxy-PAHs to an in vivo fish embryo model and points out the need for further study of their environmental occurrence and biologic effects. 相似文献
20.
Subham Mookerjee Abhishek Jaiswal Prasenjit Batabyal Marc H. Einsporn Ruben J. Lara Banwarilal Sarkar Sucharit Basu Neogi Anup Palit 《Environmental monitoring and assessment》2014,186(10):6241-6250
The Gangetic delta is a century-old cholera endemic belt where the role of riverine–estuarine ecosystem in cholera transmission has never been elucidated. Seasonality, distribution, and abundance of environmental Vibrio cholerae O1/O139 and vibriophage in Hooghly riverine–estuarine environment and their correlation with cholera incidence pattern in West Bengal, India, have been analyzed for the first time across summer, monsoon, and winter months. A total of 146 water samples collected from two sites of the Hooghly River (Howrah and Diamond Harbour) were analyzed physicochemically along with cultivable Vibrio count (CVC), V. cholerae O1/O139, and vibriophages. V. cholerae O1 was detected in 56 (38.3 %) samples, while 66 (45.2 %) were positive for V. cholerae O1 phages. Flood tide, water temperature (31?±?1.6 °C), and turbidity (≥250 nephelometric turbidity unit (NTU)) significantly stimulated V. cholerae and vibriophage abundance in riverine ecosystem. Solitary existence of V. cholerae O1 and phages (p?0.0001) in aquatic environment divulges the dominance of either of the entity (V. cholerae O1 or V. cholerae O1 Φ) on the other. Significant association (p?0.05) between Kolkata cholera cases and V. cholerae O1 in aquatic environment implies the role of riverine–estuarine ecosystem in cholera transmission. A “biomonitoring tool” of physicochemical stimulants, tidal, and climatic variants has been proposed collating V. cholerae and phage dynamics that can forewarn any impending cholera outbreak. 相似文献