Antibacterial and Drug Elution Performance of Thermoplastic Blends

Food preservatives or drug compounds can be eluted from polymer substrates to prevent the occurrence of hospital-acquired infections and food spoilage. We investigated the antimicrobial and drug-elution properties of the albumin and zein thermoplastic blends plasticized with glycerol and mixed with varying amounts of low-density polyethylene (LDPE), food preservatives (sodium benzoate or sodium nitrite), and drugs (ampicillin or ciprofloxacin). Bacillus subtilis and Escherichia coli were utilized as Gram (+) and Gram (?) species, respectively, for antimicrobial and drug-elution analyses, since these species are common in the human body and in food environments. The amount of contamination occurring in food and medical applications could be limited with usage of plastic blends made from thermomechanical molding of proteins (albumin from hen egg white and zein from corn), drug eluting compounds, and low-density polyethylene.     

Assessment of the potential hazards of nitrate contamination in surface and groundwater in a heavily fertilized and intensively cultivated district of India

We made an inventory of nitrate (NO3-N) enrichment in surface and groundwater systems in the Hooghly district of India owing to intensive farming with high fertilizer doses as a function of quantity of fertilizers use, soil characteristics, types of crop grown, depth of groundwater sampling and also N-load in soil profiles. Water samples were collected from different sources at 412 odd sites spread over in 17 blocks of the district along with representative soil profiles. On average, the study area had high clay and NO3-N in soil profiles with an increasing and decreasing trends along depth, respectively. The NO3-N content both in surface and groundwater varied from 0.01 microg mL(-1) to 4.56 microg mL(-1), being well below the threshold limit of 10 microg mL(-1) fixed by WHO for drinking purpose. The content decreased with increasing depth of wells (r = -0.39**) and clay content of soil profiles (r = -0.31**). It, however, increased with increasing rate of fertilizer application (r = 0.72**), NO3-N load in soil profiles (r = 0.85**) and was higher in areas where shallow--rather than deep-rooted crops are grown. Results indicated even under fairly high quantity of fertilizer use, groundwater of the study area is safe for drinking purpose.     

Assessment of potential hazards of fluoride contamination in drinking groundwater of an intensively cultivated district in West Bengal, India

We assessed the potential of fluoride (F) contamination in drinking groundwater of an intensively cultivated district in India as a function of its lithology and agricultural activities. Three hundred and eight groundwater samples were collected at different depths from various types of wells and analyzed for pH, EC, NO(3)-N load and F content. A typical litholog was constructed and database on fertilizer and pesticide uses were also recorded for the district. The water samples were almost neutral in reaction and non-saline in nature with low NO(3)-N content (0.02 to 4.56 microg mL(-1)). Fluoride content in water was also low (0.01 to 1.18 microg mL(-1)) with only 2.27% of them exceeding 1.0 microg mL(-1) posing a potential threat of fluorosis. On average, its content varied little spatially and along depth of sampling aquifers because of homogeneity in lithology of the district. The F content in these samples showed a significant positive correlation (r = 0.12, P < or = 0.05) with the amount of phosphatic fertilizer (single super phosphate) used for agriculture but no such relation either with the anthropogenic activities of pesticide use or NO(3)-N content, pH and EC values of the samples was found. The results suggest that the use of phosphatic fertilizer may have some role to play in F enrichment of groundwater.     

Airborne load of Cassia pollen in West Bengal, eastern India: its atmospheric variation and health impact

A Burkard personal volumetric sampler was used at Sriniketan, a town about 150 km northwest of Calcutta, in the state of West Bengal, in eastern India to record the frequency of three common airborne Cassia pollen types, Cassia tora, Cassia occidentalis, and Cassia fistula for two consecutive years (2004–2006). Correlation was made between the meteorological factors and the pollen concentration in the atmosphere. The study reports Cassia pollinosis by in vivo skin prick test in respiratory allergic patients. The highest positive reactions were exhibited by C. tora (34.7 %), C. fistula (33.3 %), and C. occidentalis (28.5 %). The allergic potential of these was investigated by in vitro enzyme linked immunosorbent assay test. Their protein components were analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, in the range of 15.8–81.5 kDa. In C. occidentalis and C. fistula, 11 bands were found, while it was 10 in C. tora. The results show that the Cassia pollen occur significantly in the atmosphere with the potential to elicit an allergic response in susceptible patients.     

Characterization of different road dusts in opencast coal mining areas of India

Dust from haul and transport roads are the major source of air pollution in opencast coal mining areas. Dust generated during mining operations pollutes air which causes different health problems. Various available techniques are implemented in the field to minimize and control dust in mining areas. However, they are not very effective because dust deposited on road surfaces are not removed by these techniques. For effective control of dust in opencast mining areas, it has to be regularly collected from road surfaces and may be converted into solid form, and subsequently can be used as a domestic fuel considering its physicochemical properties. The present paper describes a comparative study of qualitative and quantitative aspects of road dust samples of four coalfields of India. The pH of the dust was found to be in the range of 5.1–7.7. Moisture, ash, volatile matter, fixed carbon, water-holding capacity, bulk density, and specific gravity of dust samples were found to be in the range of 0.5–3.0%, 45–76%, 12.6–20.0%, 10.2–45.3%, 21.17–31.71%, 1.15–1.70, and 1.73–2.30 g cm⁻³, respectively. Observing the overall generation and characteristics of coal dust, it is suggested that coal dust from haul and transport roads of mining areas can be effectively collected and used as domestic fuel.     

Study of temporal variation in ambient air quality during Diwali festival in India

The variation in air quality was assessed from the ambient concentrations of various air pollutants [total suspended particle (TSP), particulate matter ≤10 μm (PM₁₀), SO₂, and NO₂] for pre-Diwali, Diwali festival, post-Diwali, and foggy day (October, November, and December), Delhi (India), from 2002 to 2007. The extensive use of fireworks was found to be related to short-term variation in air quality. During the festival, TSP is almost of the same order as compared to the concentration at an industrial site in Delhi in all the years. However, the concentrations of PM₁₀, SO₂, and NO₂ increased two to six times during the Diwali period when compared to the data reported for an industrial site. Similar trend was observed when the concentrations of pollutants were compared with values obtained for a typical foggy day each year in December. The levels of these pollutants observed during Diwali were found to be higher due to adverse meteorological conditions, i.e., decrease in 24 h average mixing height, temperature, and wind speed. The trend analysis shows that TSP, PM₁₀, NO₂, and SO₂ concentration increased just before Diwali and reached to a maximum concentration on the day of the festival. The values gradually decreased after the festival. On Diwali day, 24-h values for TSP and PM₁₀ in all the years from 2002 to 2007 and for NO₂ in 2004 and 2007 were found to be higher than prescribed limits of National Ambient Air Quality Standards and exceptionally high (3.6 times) for PM₁₀ in 2007. These results indicate that fireworks during the Diwali festival affected the ambient air quality adversely due to emission and accumulation of TSP, PM₁₀, SO₂, and NO_2.     

Biodegradation of Poly(ε-caprolactone) (PCL) Film by <Emphasis Type="Italic">Alcaligenes faecalis</Emphasis>

The biodegradation behavior of PCL film with high molecular weight (80,000 Da) in presence of bacterium Alcaligenes faecalis and the analysis of degraded polymer film have been carried out. Thin Films of PCL were prepared by means of solution casting method and the bacterial degradation behavior was carried in basal medium, in presence of bacteria with time variation after UV treatment. It was observed that after UV treatment the degradation of polymer film was increased and the degradation rate followed a three steps degradation mechanism. The degraded polymer film was analyzed by means of Differential Scanning Calorimeter (DSC), Thermo Gravimetric Analyzer (TGA) and Fourier Transform Infrared Spectroscope (FTIR). DSC results revealed that at the initial stages of the degradation up to 15–20 days, the bacterium preferentially degrades the amorphous parts of the polymer film over the crystalline zone. Thermo gravimetric analysis highlighted the low temperature stability of degraded films with extent of degradation. FTIR results showed the chain scission mechanism of the polymer chains and also supported the preferential degradation of amorphous phase over crystalline phase in the initial stages of the degradation.     

Removal efficiency of fluoride by novel Mg-Cr-Cl layered double hydroxide by batch process from water

The fluoride ion removal from aqueous solution using synthesized Mg-Cr-Cl layered double hydroxide has been reported.Mg-Cr-Cl was characterized by X-ray powder diffraction,Fourier-transform infrared,thermo-gravimetric analysis,differential thermal analysis,and scanning electron microscope.Adsorption experiments were carried out in batch mode as a function of adsorption dosages,contact time,pH,and initial fluoride concentration to get optimum adsorption capacity.The adsorption kinetic study showed that the adsorption process followed first order kinetics.The fluoride removal was 88.5% and 77.4% at pH 7 with an adsorbent dose of 0.6 g/100 mL solution and initial fluoride concentration of 10 mg/L and 100 mg/L,respectively.The equilibrium was established at 40 min.Adsorption experiment data were fitted well with Langmuir isotherm with R 2 = 0.9924.Thermodynamic constants were also measured and concluded that the adsorption process was spontaneous and endothermic in nature.The removal percentage decreased slowly with increasing pH.This process is suitable for industrial effluents.The regeneration of the material is not possible.