Prevalence and antimicrobial resistance of Shiga toxin-producing Escherichia coli in milk and dairy products in Egypt

Abstract

Food contaminated with Shiga toxin-producing Escherichia coli (STEC) represents a hazardous public health problem worldwide. Therefore, the present study was performed to elucidate the virulent and antimicrobial resistance characteristics of STEC isolated from milk and dairy products marketed in Egypt. A total of 125 samples (raw market milk, bulk tank milk, Kareish cheese, white soft cheese, and small scale-produced ice cream, 25 each) were collected for determination the prevalence and antimicrobial resistance profiling of STEC. Thirty-six STEC isolates were recovered from milk and dairy products. Serological analysis illustrated that three isolates were E. coli O157:H7 and 33 isolates belonged to different serotypes. Molecular examination indicated that all isolates harboured stx1 and/or stx2 genes, 14 isolates expressed eaeA gene and 3 isolates possessed rfbE gene. Antimicrobial resistance profiling of the isolates was both phenotypically and genetically examined. Interestingly, 31 out of 36 (86.11%) isolates were multidrug-resistant and harboured the extended-spectrum β-lactamase encoding genes, namely, bla_CTX-M-15, bla_SHV-12 and bla_CTX-M-14. Moreover, 12 isolates (33.33%) harboured plasmid-mediated quinolone resistant gene, qnrS. The overall conclusion of the current investigation indicated insufficient hygienic measures adopted during milking, handling, and processing leading to development of pathogenic and multidrug-resistant STEC.     

Ecosystem changes following the 2016 Kumamoto earthquakes in Japan: Future perspectives

Major earthquakes cause widespread environmental and socioeconomic disruptions that persist for decades. Extensive ground disturbances that occurred during the shallow-focus Kumamoto earthquakes will affect future sustainability of ecosystem services west of Aso volcano. Numbers of earthquake-initiated landslides per unit area were higher in grasslands than forests, likely owing to greater root reinforcement of trees, and mostly initiated on ridgelines and/or convex/planar hillslopes. Most landslides traveled short distances and did not initially evolve into debris flows; resultant sediments and wood accumulating in headwater channels can be mobilized into debris flows during future storms. Fissures along ridgelines may promote water ingress and induce future landslides and debris flows that affect residents downstream. Native grasses are at risk because of habitat fragmentation caused by ground disturbances, extensive damage to rural roads, and abandonment of traditional pasture management practices. Sustainable management of affected areas needs to consider future risk of cascading hazards and long-term socioeconomic impacts.     

Evaluation of thermally treated asbestos based on fiber number concentration determined by transmission electron microscopy

Appropriate treatment of asbestos waste is a significant problem. In Japan, inertization of asbestos-containing waste by novel techniques approved by the Ministry of the Environment is now promoted. A quantitative method of testing with high sensitivity to the asbestos levels present in the inertization products is required for the approval process, but many testing methods are only qualitative. Thus, we have developed an evaluation method for asbestos in inertized products, consisting of the extraction of fibers from inertized products and determination of fiber number concentration by transmission electron microscopy. We adopted this testing method to evaluate thermally treated asbestos. It was found that fiber number concentrations of thermally treated asbestos decreased with increased treatment temperature, and were below the environmental level (10² Mf/g) at more than 1000 °C for chrysotile and crocidolite and more than 1400 °C for amosite and other amphibole forms of asbestos.     

Mechanical properties of shotcrete with addition of sugarcane bagasse fiber and ash

Journal of Material Cycles and Waste Management - Shotcrete is a sprayed concrete placed on steep slopes that have been widely used because it can prevent surface weathering and erosion, and in...     

PREDICTING WILDERNESS SNOW WATER EQUIVALENT WITH NONWILDERNESS SNOW SENSORS1

ABSTRACT: Ten pairs of snow sensors were analyzed to investigate the feasibility of predicting snow water equivalent at high-elevation, telemetered snow sensor sites from lower-elevation sensors. The need for this analysis stems from an agreement between the California Department of Water Resources and the USDA Forest Service to temporarily allow snow sensors in California's wilderness areas so that a predictive relationship can be developed. After 10 or 15 years, the agreement calls for the sensors to be removed. Initial efforts to a priori select sensor pairs were based on proximity, colocation within a basin, and annual precipitation amount, but regression yielded poor fits (R² < 0.65) and high standard errors in eight of the ten cases. Analysis of the results suggested that eleva-tional similarity was the most important selection criteria, and that all available sensors near the target site should be analyzed via a regression screening. Using elevation for selection and the regression screening, five sensors that initially had poor fits were reanalyzed. Each of the five sensors was paired with between two and five new sensors, and R² values improved between 27 and 46 percent. Various data smoothing and editing algorithms were evaluated, but they rarely resulted in improved fits.     

Urinary 8-hydroxy-2'-deoxyguanosine in inhabitants chronically exposed to arsenic in groundwater in Cambodia

Arsenic concentrations in hair and urine, and urinary levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage, were examined for inhabitants of the Mekong Basin in Kratie Province, Cambodia. Also, the arsenic levels of tube-well water were determined. Total arsenic concentrations in tube-well water ranged from <1 to 886 microg L(-1), and 44.8% of these exceeded the WHO drinking water guideline of 10 microg L(-1). Elevated levels of arsenic were observed in the human hair and urine, and also a significant positive correlation was observed between the concentrations in hair and urine. These results suggest that the inhabitants are chronically exposed to arsenic through drinking the tube-well water. Levels of urinary 8-OHdG were higher for the subjects with higher arsenic levels in hair and urine, suggesting that induction of oxidative DNA damage was caused by chronic exposure to arsenic in tube-well water for the inhabitants in Kratie Province. To our knowledge, this is the first report on the oxidative DNA damage caused by chronic exposure to arsenic in groundwater for the inhabitants in Cambodia.     

Does calcite encrustation in Chara provide a phosphorus nutrient sink?

We studied the effect of calcite encrustation in stoneworts (Chara spp.) on P cycling in an aquatic ecosystem. Sequential fractionation was performed to quantify P fractions of the internodes of calcified (Ca-CF) and uncalcified (UCa-CF) Chara fibrosa Agardh ex Bruzelius. Our results showed that Ca-CF was able to store more P and about 14 to 23% of total P in Ca-CF was co-precipitated with encrusted calcite, while only 2 to 3% was found in UCa-CF. Furthermore, in Ca-CF, an increased amount of total P did not result in a higher release of bioavailable water-soluble and sodium hydroxide-extractable P. Extracellular calcification in Chara enhanced nutrient sink for P, provided a further bottom-up control of phytoplankton, and should be regarded as a positive feedback in stabilizing Chara dominance in lakes.     

Determination of nitrous oxide, methane, and ammonia emissions from a swine waste composting process

The amounts of harmful gas emissions from the process of composting swine waste were determined using an experimental composting apparatus. Forced aeration (19.2–96.1 l/m³/min) was carried out continuously, and exhaust gases were collected and analyzed periodically. With weekly turning and the addition of a bulking agent in order to decrease the moisture content and increase air permeability, the temperature of most of the contents rose to 70°C and composting was complete within 3–5 weeks. NH₃, CH₄, and N₂O emissions were high in the early stage of composting. About 10%–25% of the nitrogen in the raw material was lost as NH₃ gas during composting. The emission rate of NH₃ mainly depended on the aeration rate, so that as the aeration rate rose, the level of NH₃ emissions increased. The CH₄ and N₂O emissions could be kept lower with adequate treatment at more than 40 l/m³/min aeration. N₂O may be mainly the result of the denitrification of NO _x -N in the additional matured compost used as a composting accelerator. Received: September 11, 1998 / Accepted: November 8, 1999     

Development of a dynamic transfer model of (14)C from the atmosphere to rice plants

A dynamic compartment model was investigated to describe ¹⁴C accumulation in rice plants exposed to atmospheric ¹⁴C with temporally changing concentrations. In the model, rice plants were regarded to consist of three compartments: the ear and the mobile and immobile carbon pools of the shoot. Photosynthetically fixed carbon moves into the ear and the mobile carbon pool, and these two compartments release a part of this carbon into the atmosphere by respiration. Carbon accumulated in the mobile carbon pool is redistributed to the ear, while carbon transferred into the immobile carbon pool from the mobile one is accumulated there until harvest. The model was examined by cultivation experiments using the stable isotope, ¹³C, in which the ratios of carbon photosynthetically fixed at nine times during plant growth to the total carbon at the time of harvest were determined. The model estimates of the ratios were in relatively good agreement with the experimental observations, which implies that the newly developed compartment model is applicable to estimate properly the radiation dose to the neighboring population due to an accidental release of ¹⁴C from nuclear facilities.     

Promoting Sustainability with Ecological, Economic and Social Dimensions in Developing Countries

Sustainable development is one of the most important ideas, and goals of our time. It is defined as ＂development which meets the needs and aspirations of the current generation and develops safe and sound society for future generation＂. Achieving sustainable development thus involves a vigorous and urgent debate on different dimensions. The different dimensions of sustainability as a framework involve all issues such as science, technology, economic growth and development, health, education, finance investment and trade, politics, natural disasters, population growth and terrorism, etc. No single dimension is responsible to develop sustainable society. This article mainly described the integrated relationship among the three dimensions ofsustainability.