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71.
Complete degradation of butyl benzyl phthalate by a defined bacterial consortium: role of individual isolates in the assimilation pathway 总被引:3,自引:0,他引:3
Two bacterial strains, in consortium, were isolated by enrichment techniques from municipal waste-contaminated soil, which utilized butyl benzyl phthalate (BBP) as the sole carbon source. One of the isolates was identified as Arthrobacter sp. strain WY and the other one as Acinetobacter sp. strain FW based on the morphological, nutritional and biochemical characteristics and 16S rRNA sequence analysis. Various metabolites of BBP engendered by Arthrobacter sp. strain WY were isolated and identified by a combination of chromatographic and spectrophotometric analyses, which revealed a pathway involving monobutylphthalate (MBuP), monobenzyl phthalate (MBzP), phthalic acid and protocatechuic acid. The protocatechuic acid in turn was processed by ortho-cleavage dioxygenase to form beta-carboxy-cis,cis-muconate, ultimately leading to the TCA cycle. The Arthrobacter sp. strain WY could not utilize the hydrolyzed alcohols of BBP. On the other hand, the Acinetobacter sp. strain FW, which by itself could not utilize BBP as the sole carbon source, is capable of utilizing the hydrolyzed alcohols of BBP. Benzyl alcohol was found to be metabolized by the Acinetobacter sp. strain FW via benzaldehyde, benzoic acid and catechol. Catechol was further degraded by ortho-cleavage dioxygenase to cis,cis-muconic acid and subsequently to muconolactone leading to beta-ketoadipate pathway. Moreover, the Acinetobacter sp. strain FW metabolized 1-butanol through butyraldehyde and butyric acid leading to the tricarboxylic acid cycle via beta-oxidation pathway. This is the first report on the complete degradation of BBP by a defined consortium describing the role of its individual constituents in the BBP assimilation pathway. 相似文献
72.
Distribution of detoxifying genes polymorphism in Maharastrian population of central India 总被引:1,自引:0,他引:1
Saravana Devi S Vinayagamoorthy N Agrawal M Biswas A Biswas R Naoghare P Kumbhakar S Krishnamurthi K Hengstler JG Hermes M Chakrabarti T 《Chemosphere》2008,70(10):1835-1839
The present study is aimed at evaluating the genotype frequency of detoxifying genes such as GSTM1, GSTT1 and NQO1 in Maharastrian population of central India. The study revealed about 64.6% of GSTM1-positive and 35.4% GSTM1-null population. GSTT1-positive genotype was found to be 87.5% and GSTT1-null showed 12.5%. The NQO1 genotype of Maharastrian population showed 52.3% of C/C, 42.48% C/T and 5.18% T/T. The NQO1 of this population does not deviate from the expected Hardy-Weinberg equilibrium. The genotype frequencies GSTM1 and GSTT1 of the population when compared with other ethnic groups of Asia and Caucasians show distinct nature of Maharastrian population from other Asian and Caucasian population. 相似文献
73.
Antonia Engert Shumon Chakrabarti Nadine Saul Michal Bittner Ralph Menzel Christian E.W. Steinberg 《Chemosphere》2013,90(7):2136-2141
For organisms, temperature is one of the most important environmental factors and gains increasing importance due to global warming, since increasing temperatures may pose organisms close to their environmental tolerance limits and, thus, they may become more vulnerable to environmental stressors. We analyzed the temperature-dependence of the water-soluble antioxidant capacity of the cladoceran Moina macrocopa and evaluated its life trait variables with temperature (15, 20, 25, 30 °C) and humic substance (HS) concentrations (0, 0.18, 0.36, 0.90, 1.79 mM DOC) as stressors. Temperatures below and above the apparent optimum (20 °C) reduced the antioxidative capacity. Additions of HSs increased body length, but decreased mean lifespan at 15 and 20 °C. There was no clear HS-effect on offspring numbers at 15, 20, and 30 °C. At 25 °C with increasing HS-concentration, lifespan was extended and offspring numbers increased tremendously, reaching 250% of the control. Although the applied HS preparation possesses estrogenic and antiandrogenic activities, a xenohormone mechanism does not seem plausible for the reproductive increase, because comparable effects did not occur at other temperatures. A more convincing explanation appears to be the mitohormesis hypothesis which states that a certain increase of reactive oxygen production leads to improved health and longevity and, with Moina, also to increased offspring numbers. Our results suggest that at least with the eurythermic M. macrocopa, a temperature above the optimum can be beneficial for several life trait variables, even when combined with a chemical stressor. Temperatures approximately 10 °C above its optimum appear to adversely affect the lifespan and reproduction of M. macrocopa. This indicates that this cladoceran species seems to be able to utilize temperature as an ecological resource in a range slightly above its thermal optimum. 相似文献
74.
Pal Madhubonti Mondal Mrinal Kanti Paine Tapan Kanti Pal Parimal 《Environmental science and pollution research international》2018,25(17):16579-16589
Environmental Science and Pollution Research - A novel graphene-based nanocomposite membrane was synthesized by interfacial polymerization (IP) through chemical bonding of the graphene oxide (GO)... 相似文献
75.
Juwarkar AA Yadav SK Thawale PR Kumar P Singh SK Chakrabarti T 《Environmental monitoring and assessment》2009,157(1-4):471-481
An important goal of ecological rehabilitation is to accelerate natural successional processes to increase biological productivity, soil fertility and biotic control over biogeochemical fluxes within the recovering ecosystems. A new approach called Microbe Assisted Green Technology (MAGT) is an integrated biotechnological approach developed at National Environmental Engineering Research Institute (NEERI) through exhaustive laboratory as well as field studies and serve as a model for land reclamation and development of lush green vegetation on mine overburdens. One year old seedlings of native tree species were planted on 6.3 ha area of manganese mine overburden at Gumgaon under Manganese Ore India Ltd., Maharashtra, India. Continuous efforts resulted in nutrient rich soil with high N, P, K and organic carbon; well developed biodiversity, including bacteria, fungi, higher plants (more than 350 species) and different classes of animals. Planted trees accumulated 698 t ha( - 1) above ground biomass and 143 t ha( - 1) below ground mass. This was achieved in 18 years by MAGT, which otherwise takes hundreds of years. 相似文献
76.
Padma S. Rao Vasant A. Mhaisalkar A. Shrivastava Animesh Kumar T. Chakrabarti S. Devotta 《Environmental monitoring and assessment》2010,168(1-4):55-61
Plantation or green belt (GB) grown around the factories and industrial premises improves the condition of land, mitigates air pollution (as the plants serve as a sink for pollutants and check the flow of dust, etc.), and reduces the level of noise pollution. The software developed by NEERI for the determination of the optimal width of GB in and around an industry is based on the pollution attenuation coefficient of selected plant species of deciduous trees existing in the region. The assessment of the impact of these plantations/green belts of 500-m width in and around a petroleum refinery in the west coast of India is evaluated for reducing/managing various waste generated, and it is observed that the overall efficiency is more than 60%. 相似文献
77.
Alka Dhondge Subin Surendran Muhil Vannan Seralathan Pravin K. Naoghare Kannan Krishnamurthi Sivanesan Saravana Devi Tapan Chakrabarti 《Environmental science and pollution research international》2012,19(9):4030-4041
Purpose
There are many arguments on the carcinogenic potential of bitumen extract. The mechanism of bitumen-induced damage is not well understood at the molecular level. Therefore, in the present study, cell-transforming and tumor-inducing potential of bitumen extract was studied using in vitro [human osteosarcoma (HOS) cells] and in vivo [nude and severe combined immunodeficiency (SCID) mice] models.Methods
Gas chromatography/mass spectrometry (GC/MS) analysis was carried out to find out the existence of carcinogenic compounds in the bitumen extract. Cell transformation test, anchorage independence assay, karyotyping assay, tumorigenicity assay, and 2-DE analysis were used to find out the effect of bitumen using the in vitro and in vivo models.Results
GC/MS analysis showed the existence of carcinogenic compounds in the bitumen extract. HOS cells were treated with different concentrations (25, 50, and 100???l/ml) of bitumen extract. Compared to the parental HOS cells, bitumen transformants (HOS T1 and HOS T2) showed the characteristics of anchorage independency, chromosomal anomaly, and cellular transformation. Interestingly, bitumen transformants were not able to form tumor in nude/SCID mice. Proteomic analysis revealed the existence of 19 differentially expressed proteins involved in progression of cancer, angiogenesis, cell adhesion, etc.Conclusions
Exposure of bitumen extract to HOS cells results in the cellular transformation similar to cancer cells and can modulate proteins involved in the progression of cancer. We state that the non-tumorogenic potential of bitumen transformant in nude/SCID mice can be attributed to the downregulation of galectin-1, chromodomain helicase DNA-binding protein 1-like gene, and membrane-associated guanylate kinase 2 protein. 相似文献78.
E-waste scenario in India, its management and implications 总被引:1,自引:0,他引:1
Sushant B. Wath P. S. Dutt T. Chakrabarti 《Environmental monitoring and assessment》2011,172(1-4):249-262
Electronic waste or E-waste comprises of old, end-of-life electronic appliances such as computers, laptops, TVs, DVD players, refrigerators, freezers, mobile phones, MP3 players, etc., which have been disposed of by their original users. E-waste contains many hazardous constituents that may negatively impact the environment and affect human health if not properly managed. Various organizations, bodies, and governments of many countries have adopted and/or developed the environmentally sound options and strategies for E-waste management to tackle the ever growing threat of E-waste to the environment and human health. This paper presents E-waste composition, categorization, Global and Indian E-waste scenarios, prospects of recoverable, recyclable, and hazardous materials found in the E-waste, Best Available Practices, recycling, and recovery processes followed, and their environmental and occupational hazards. Based on the discussion, various challenges for E-waste management particularly in India are delineated, and needed policy interventions were discussed. 相似文献
79.
Grover Madhuri Behl Tapan Sachdeva Monika Bungao Simona Aleya Lotfi Setia Dhruv 《Environmental science and pollution research international》2021,28(15):18893-18907
Environmental Science and Pollution Research - Curcumin is a polyphenolic compound that exhibited good anticancer potential against different types of cancers through its multi-targeted effect like... 相似文献
80.
Uday Kumar Chakrabarti Jigisha K. Parikh 《Journal of Loss Prevention in the Process Industries》2013,26(5):941-947
Following a toxic hazmat release, decisions need to be made vis-a-vis a safe evacuation distance for population along the transportation route, or whether to shelter-in-place if there is insufficient time to conduct an evacuation. The first responder may have to take these decisions through a quick estimation of the Initial Isolation Zone (IIZ) and protective action distances based on assumed rate of releases. Real-time evacuation planning models could be used for effective evacuation purposes. However, factors that usually were taken into account include weather conditions, population density, time of day, and uncertainty about the chemicals involved and/or the accumulating concentrations in public areas. Emergency planning including evacuation in India is generally in line with UNEP's APELL (Awareness and Preparedness for Emergencies at Local Level) methodology. However, quantitative risk based assessment is catching up particularly in large industrial zones. The paper highlights a case study of the evaluation of identified seven critical evacuation planning zones along three highway study routes in and around the industrial city of Surat in western India. The elapsed time after a release is estimated through ALOHA and evacuation travel time estimates are made for each zone. For identified scenarios, evacuation of IIZ, generally comprising of road side population, is effectively possible in six out of seven identified emergency planning zones except in zone-7, where certain people would still be exposed, as full evacuation may not be achieved. However, shelter-in-place would be advisable for population residing in permanent settlements within the protective action zone. 相似文献