氨态氮对菲律宾蛤仔毒理机制研究的内参基因筛选

为了准确和系统地分析NH_3-N对菲律宾蛤仔(Ruditapes philippinarum)毒性的分子机制,并对相关基因的转录组学数据进行验证,利用ge Norm和Normfinder这2种软件,以暴露30 d中不同时间点的鳃组织c DNA为模板,从18S r RNA(18S)、beta-actin(Actin)、beta-tubulin(Tubu)、elongation factor 1-alpha(EF1α)、cyclophilin A(Cy PA)、Ubiquitin(Ub)等6个备选管家基因中,筛选可以稳定表达的内参基因,作为转录组学分析的内参基因。以稀释50倍的鳃组织c DNA模板作为检测模板,ge Norm软件分析获得6对备选内参基因的表达稳定性依次为:EF1αCy PAActinTubuUb18S,较优内参为EF1α和Cy PA;Norm Finder软件分析获得6对备选内参基因的表达稳定性为:EF1αActinTubuCy PAUb18S,最优内参基因为EF1α。为了验证上述筛选结果,分别以EF1α和Cy PA作为内参基因研究unigene1的表达趋势,发现以EF1α为内参基因时,unigene1的q RT-PCR的表达趋势与其转录组表达倍数的变化趋势一致。因此确定NH_3-N暴露菲律宾蛤仔后,鳃组织中表达最为稳定的内参基因为EF1α。     

The impact of airborne particulate matter on pediatric hospital admissions for pneumonia among children in Jinan,China: A case-crossover study

This study aims to examine the effect of short-term changes in the concentration of particulate matter of diameter ≤2.5 µm (PM_2.5) and ≤10 µm (PM₁₀) on pediatric hospital admissions for pneumonia in Jinan, China. It explores confoundings factors of weather, season, and chemical pollutants. Information on pediatric hospital admissions for pneumonia in 2014 was extracted from the database of Jinan Qilu Hospital. The relative risk of pediatric hospital admissions for pneumonia was assessed using a case-crossover approach, controlling weather variables, day of the week, and seasonality. The single-pollutant model demonstrated that increased risk of pediatric hospital admissions for pneumonia was significantly associated with elevated PM_2.5 concentrations the day before hospital admission and elevated PM₁₀ concentrations 2 days before hospital admission. An increment of 10 μg/m³ in PM_2.5 and PM₁₀ was correlated with a 6% (95% CI 1.02–-1.10) and 4% (95% CI 1.00–1.08) rise in number of admissions for pneumonia, respectively. In two pollutant models, PM_2.5 and PM₁₀ remained significant after inclusion of sulfur dioxide or nitrogen dioxide but not carbon monoxide. This study demonstrated that short-term exposure to atmospheric particulate matter (PM_2.5/PM₁₀) may be an important determinant of pediatric hospital admissions for pneumonia in Jinan, China.

Implications: This study demonstrated that short-term exposure to atmospheric particulate matter (PM_2.5/PM₁₀) may be an important determinant of pediatric hospital admissions for pneumonia in Jinan, China, and suggested the relevance of pollutant exposure levels and their effects. As a specific group, children are sensitive to airborne particulate matter. This study estimated the short-term effects attribute to other air pollutants to provide references for relevant studies.     

生活生产类产品VOCs含量限值法规与标准研究

本文对国内生活生产类产品VOCs含量限值的国家标准、地方标准、行业标准以及中国香港、欧美等发达地区精细化的生活生产类产品VOCs含量限值法规和标准做了比较全面的综述,并从产品限值种类以及限值要求两个方面进行了对比研究和分析,提出了我国生产生活类产品VOCs 含量限值法规标准制定建议。笔者认为,应借鉴发达国家成功的生活生产类产品VOCs含量限值法规及标准制定经验,从做好标准和产品技术要求衔接、建立区域协同制定标准模式、结合经济因素或环境空气质量,分步推进相关标准制定和发布等方面入手,建立符合实际国情的产品种类较为全面、标准要求较高的生产生活类产品VOCs含量限值法规与标准体系,尤其是生活消费品方面,减少直接与人体接触的生活消费品VOCs排放。     

亲疏水性溶解性有机物对聚偏氟乙烯超滤膜污染行为

选用模拟亲疏水性有机物及实际二级处理水中的亲疏水性有机物,分别进行了聚偏氟乙烯(PVDF)超滤膜污染实验.并测定了不同污染物与膜及污染物间的作用力,分析亲/疏水性有机物对PVDF超滤膜的污染行为.结果表明,膜-污染物及污染物间相互作用力是控制膜污染行为的主导因素.亲水性有机物与PVDF超滤膜之间的相互亲和力较强,致使运行初期膜通量的大幅度下降,是PVDF膜的优势污染物.但是,针对实际污染物,运行后期,疏水性有机物之间较强的相互作用力使得其取代了亲水性有机物,成为膜的优势污染物.此外,模拟亲疏水性有机物的膜污染变化规律与实际亲疏水性有机物相似,表明使用模拟污染物可一定程度上预测实际废水中相应性能污染物的膜污染行为.     

Application of canonical correspondence analysis to determine the ecological contribution of phytoplankton to PCBs bioaccumulation in Qinhuai River,Nanjing, China

The distribution and interactions of phytoplankton and 14 polychlorinated biphenyls (PCBs) were investigated using canonical correspondence analysis in autumn in the Qinhuai River, Nanjing, China. Concentrations of PCBs in water and algal samples ranged from 33.78 to 144.84 ng/L and from 0.21 to 19.66 ng/L (0.06 to 3.04 ng/mg biomass), respectively. The predominant residual species of PCBs in water samples were tri- through hexachlorobiphenyls, and the predominant residuals in algae were tri-, tetra-, and heptachlorobiphenyls. The degree of eutrophication affected phytoplankton composition and PCB bioaccumulation, and led to sample site- and algal species specificity of PCB residues in the study area. Chlorophyta, Bacillariophyta, and Euglenophyta had strong capacities to take up PCBs, whereas Cyanophyta was less involved in the transfer of these compounds. Bioaccumulation of PCBs by algae may be affected by water quality, chlorination, phytoplankton composition, and the structure of the PCBs and the algal cell walls.     

Analyses of combined effects of cytostatic drugs on micronucleus formation in the <Emphasis Type="Italic">Tradescantia</Emphasis>

Recent experiments showed that 5-fluorouracil (5FU), cisplatin (CDDP), etoposide (ET), and imatinib mesylate (IM), which are currently among the most widely used anticancer drugs, cause damage of the genetic material in higher plants. The aim of the present study was to determine whether mixtures of these drugs cause synergistic or antagonistic effects which may have an impact on their environmental safety. Therefore, the effects of binary mixtures of these anticancer drugs on the induction of micronuclei (MN) which reflect structural and numerical chromosomal aberrations were assessed in Tradescantia tetrads. Synergistic/antagonistic effects were determined by comparison with single exposures that would be equally effective in a reference model of independent action. This comparison was performed at two distinct effect sizes. We found clear evidence for synergisms in combination experiments with IM and antagonism in a high-dose experiment with ET and 5FU. Our findings indicate that IM increases the genotoxic effects of other anticancer drugs. The maximal effects which we found were in the range between 19 and 38 % in the excess of effect sizes predicted under independent action. These effects may have an impact on the overall genotoxic activities of untreated hospital waste waters but not on the environment in general as the predicted environmental concentrations of the studied drugs are several orders of magnitude lower as the levels which are required to cause induction of MN in higher plants.     

Physiological and biochemical responses of rice seeds to phosphine exposure during germination

Rice seeds (Tianyou, 3618) were used to examine the physiological and biochemical responses to phosphine exposure during germination. A control (0 mg m⁻³) and four concentrations of phosphine (1.4 mg m⁻³, 4.2 mg m⁻³, 7.0 mg m⁻³ and 13.9 mg m⁻³) were used to treat the rice seeds. Each treatment was applied for 90 min once per day for five days. The germination rate (GR); germination potential (GP); germination index (GI); antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT); and lipid peroxidation measured through via malondialdehyde (MDA) were determined as indicators of the physiological and biochemical responses of the rice seeds to phosphine exposure. These indicators were determined once per day for five days. The results indicated that the GR, GP and GI of the rice seeds markedly decreased after phosphine exposure. The changes in the activities of the antioxidant enzymes due to the phosphine exposure were also significant. The exposure lowered the CAT and SOD activities and increased POD activity in the treated rice seeds compared with controls. The MDA content exhibited a slow increase trend with the increase of phosphine concentration. These results suggest that phosphine has inhibitory effects on seed germination. In addition, phosphine exposure caused oxidative stress in the seeds. The antioxidant enzymes could play a pivotal role against oxidative injury. Overall, the effect of phosphine on rice seeds is different from what has been reported previously for insects and mammals.     

The use of experimental data and their uncertainty for assessing ozone photochemistry in the Eastern Iberian Peninsula

Observation-based methods are useful tools to explore the sensitivity of ozone concentrations to precursor controls. With the aim of assessing the ozone precursor sensitivity in two locations: Paterna (suburban) and Villar del Arzobispo (rural) of the Turia river basin in the east of Spain, the photochemical indicator O₃/NO_y and the Extent-of-Reaction (EOR) parameter have been calculated from field measurements. In Paterna, the O₃/NO_y ratio varied from 0 to 13 with an average value of 5.1 (SD 3.2), whereas the averaged value for the EOR was 0.43 (SD 0.14). In Villar del Arzobispo, the O₃/NO_y ratio changed from 5 to 30 with a mean value of 13.6 (SD 4.7) and the EOR gave an averaged value of 0.72 (SD 0.11). The results show two different patterns of ozone production as a function of the location. The suburban area shows a VOC-sensitive regime whereas the rural one shows a transition regime close to NO_x-sensitive conditions. No seasonal differences in these regimes are observed along the monitoring campaigns. Finally, an analysis of the influence of the measurement quality of NO_y, NO_x and O₃ on the uncertainty of the O₃/NO_y ratio and the EOR was performed showing that the uncertainty of O₃/NO_y is not dependent on either its value or the individual values of O₃ and NO_y but just on the quality of O₃ and NO_y measurements. The maximum uncertainty is 26% as long as the combined uncertainties of O₃ and NO_y remain below the 7.5%. The case of the EOR is different and its uncertainty depends on both the value of the EOR parameter and the individual concentration values of NO_y and NO_x. The uncertainty of the EOR estimation can be very high (>200%) if the combined uncertainties of both NO_y and NO_x are high (>7.5%), or especially, if u(NO_y) and u(NO_x) differ considerably from each other (>3.5%).     

Photolytic degradation of decabromodiphenyl ethane (DBDPE)

The photolytic degradation of decabromodiphenyl ethane (DBDPE), an alternative flame retardant to decabromodiphenyl ether, was investigated in a variety of matrixes (n-hexane, tetrahydrofuran, methanol/water, humic acid/water, and silica gel) by irradiation under ultraviolet light and in n-hexane under natural light. Photolytic degradation of DBDPE occurs in all the matrixes investigated within the irradiation period (<320 min). The degradation experiments showed varied reaction rates, dependent on the matrixes, with increasing half-lives (t_1/2) in the order of tetrahydrofuran (t_1/2 = 6.0 min) > n-hexane (t_1/2 = 16.6 min) > humic acid/water (30 < t_1/2 < 60) > silica gel (t_1/2 = 75.9 min) > methanol/water (t_1/2 > 240 min). The reaction in tetrahydrofuran, n-hexane, and silica gel matrixes can be described by the pseudo first order kinetics. Nevertheless, the matrixes have little effect on the degradation product distributions of DBDPE. A numbers of debrominated intermediates were identified. The degradation involves the initial formation of nona-BDPEs and the subsequent decomposition of these congeners to lower brominated congeners (octa- and hepta-BDPEs) within the irradiation time. To our knowledge, the present work is the first attempt to investigate the photolytic degradation kinetics and the identification of intermediates, as well as the degradation mechanism, during the degradation of DBDPE. Further research is needed to understand the photolytic degradation pattern of DBDPE in the natural environment.     

Influence of temperature changes on ambient air NO<Subscript>x</Subscript> chemiluminescence measurements

Users of automatic air pollution monitors are largely unaware of how certain parameters, like temperature, can affect readings. The present work examines the influence of temperature changes on chemiluminescence NO(x) measurements made with a Thermo Scientific 42i analyzer, a model widely used in air monitoring networks and air pollution studies. These changes are grouped into two categories according to European Standard EN 14211: (1) changes in the air surrounding the analyzers and (2) changes in the sampled air. First, the sensitivity tests described in Standard EN 14211 were performed to determine whether the analyzer performance was adapted to the requirements of the standard. The analyzer met the performance criteria of both tests; however, some differences were detected in readings with temperature changes even though the temperature compensator was on. Sample temperature changes were studied more deeply as they were the most critical (they cannot be controlled and differences of several tens of degrees can be present in a single day). Significant differences in readings were obtained when changing sample temperature; however, maximum deviations were around 3% for temperature ranges of 15°C. If other possible uncertainty contributions are controlled and temperature variations with respect to the calibration temperature are not higher than 15°C, the effect of temperature changes could be acceptable and no data correction should have to be applied.