Exploring the abundance and influencing factors of antimicrobial resistance genes in manure plasmidome from swine farms

Plasmids play a critical role in the dissemination of antimicrobial resistance genes (ARGs), however, a systematical understanding of ARGs originated from plasmids in swine production is currently lacking. Herein, quantitative polymerase chain reaction was applied to determine the prevalence of ten ARGs and the class1 integron gene intI1 of plasmid source in swine manure from 44 farms in Sichuan, Hubei and Hebei provinces, China. All assayed ARGs were observed in plasmid DNA samples, and the average absolute abundance of aac(6’)-Ib-cr, bla_NDM, bla_CTX-M, optrA, ermB, floR, mcr-1, qnrS, tetM, sul1 and intI1 were 7.09, 2.90, 4.67, 6.62, 7.55, 7.14, 4.08, 4.85, 7.16, 7.11 and 8.07 of 10 log copies/gram, respectively. IntI1 showed a high correlation (r > 0.8, P < 0.01) with the abundance of aac(6’)-Ib-cr and sul1 in swine manure. Moreover, the farm scale (i.e., herd population) and geographical location were not found to be critical factors influencing the absolute abundance of ARGs of plasmid DNA in swine farms. However, the concentrations of florfenicol, Cu, Zn, Fe, total phosphorus (TP) and total potassium (TK) demonstrated a significant correlation with the abundance of several ARGs. Particularly, Cu and Zn had high correlations with optrA and bla_CTX-M, respectively. Our results demonstrated that antibiotics, heavy metals and environmental nutrients are likely jointly contributing to the long-term persistence of ARGs in swine production. This study provides insights into the abundance and influencing factors of ARGs from swine manure, which is of significance for assessing and reducing the public health risks in livestock production.     

基于ARIMA与LS-SVM组合模型的飞行事故征候预测

应用差分自回归移动平均模型(ARIMA)和最小二乘支持向量机(LS-SVM)的组合模型,对某航空公司的月度事故征候万时率进行了预测分析。对2008—2016年某航空公司的事故征候、飞行小时、航空器数量等历史数据建立ARIMA模型,应用SPSS软件进行模型拟合,获得事故征候万时率的线性部分;随后利用LS-SVM分析ARIMA模型的残差,获取非线性部分,最终通过二者之和获得ARIMA+LSSVM组合模型。对2017年1—3月的月度事故征候万时率进行了预测,并用实际数据验证。结果表明:ARIMA(1,1,1)(1,1,1)12模型较好地拟合了事故征候万时率的历史序列,LS-SVM模型对残差的拟合获得了较好的精度;组合模型的短期(3个月)预测值与航空公司事故征候万时率的趋势完全一致,且预测精确度可接受。     

Sorption of tylosin and sulfamethazine on solid humic acid

Tylosin(TYL) and sulfamethazine(SMT) are ionizable and polar antimicrobial compounds,which have seeped into the environment in substantial amounts via fertilizing land with manure or sewage. Sorption of TYL and SMT onto humic acid(HA) may affect their environmental fate. In this study, the sorption of TYL and SMT on HA at different conditions(pH, ionic strength) was investigated. All sorption isotherms fitted well to the Henry and Freundlich models and they were highly nonlinear with values of n between 0.5 and 0.8, which suggested that the HA had high heterogeneity. The sorption of TYL and SMT on HA decreased with increasing p H(2.0–7.5), implying that the primary sorption mechanism could be due to cation exchange interactions between TYL~+/SMT~+ species and the functional groups of HA.Increasing ionic strength resulted in a considerable reduction in the K_d values of TYL and SMT,hinting that interactions between H bonds and π–π EDA might be an important factor in the sorption of TYL and SMT on HA. Results of Fourier transform infrared(FT-IR) and ~13C-nuclear magnetic resonance(NMR) analysis further demonstrated that carboxyl groups and O-alkyl structures in the HA could interact with TYL and SMT via ionic interactions and H bonds,respectively. Overall, this work gives new insights into the mechanisms of sorption of TYL and SMT on HA and hence aids us in assessing the environmental risk of TYL and SMT under diverse conditions.     

海水对含水层沉积物中镭解吸的模拟实验

本研究采用莱州湾沿岸含水层中的沉积物,通过渗滤实验模拟镭的解吸过程。结果表明,在典型的地下水流速范围内(0.02~0.30 cm/min),镭同位素(223Ra、224Ra、226Ra)的解吸没有显著变化。在海水与淡水的混合过程中,解吸的镭比活度与水的盐度呈显著的线性正相关关系。当盐度超过海水盐度时(如S=80的卤水中),解吸的镭比活度则趋于最值平台。对比模拟估算的地下水中镭的比活度与莱州湾沿岸实测间隙水中镭的比活度,223Ra和224Ra的结果基本一致,而226Ra的实测结果较模拟估算结果偏小。可能由于含水层中226Ra经反复解吸后得不到母体核素及时补充所致。本研究将为更好地理解镭同位素在含水层中的地球化学行为提供科学依据,为合理地选择量化海底地下水排放(submarine groundwater discharge,SGD)模型的镭端元值提供新的参考视角。     

224Ra对春季青岛近海水体混合及营养盐来源的示踪研究

2013年3月,以青岛近海为研究区域,分析了过剩224Ra(224Raex)的分布特征及其影响因素,并以之为示踪工具对水体的混合过程和营养盐来源进行了评估。研究发现,224Raex的比活度范围为1.30.2 dpm/100 L到10.21.3 dpm/100 L,并呈现出近岸高、远岸低和低潮时高、高潮时低的时空分布特征。224Raex-盐度对研究区域的水体混合状况的指示作用明显优于温度-盐度指标。2013年3月青岛近海溶解无机磷(DIP)和溶解硅(DSi)浓度的主控因素是潮汐作用,而溶解无机氮(DIN)的来源组成较为复杂,可能包括河流输入、生活污水、工业废水、潮汐混合、地下水排放等。     

A Comparison Study of Wheat Gluten Composites Filled with Dialdehyde Starch and Native Starch

Environmentally friendly green composites were prepared by blending Wheat gluten (WG) as matrix, dialdehyde starch (DAS) as filler and glycerol as plasticizer followed by compression molding of the mixture at 110 °C. The properties of the WG/DAS composite are compared with those of the WG/native wheat starch (NWS) composites. While tensile strength and strain at break decrease with increasing NWS content in the WG/NWS composites, a small content of DAS could improve tensile strength and strain at break simultaneously in the WG/DAS composites. The WG/DAS composites exhibit reduced moisture absorption in comparison with the WG/NEW composites. Formation of chemical bonding between DAS and WG is beneficial for the dispersion of DAS in the WG matrix and WG/DAS composites exhibit improved mechanical properties and reduced moisture absorption over the WG/NWS composites.     

Seasonal variation of mercury concentrations in organisms of the Cilician Basin

Total mercury concentrations in organisms typical of those found in the Cilician Basin of the northeastern Mediterranean have been measured from 1977 to the present. These concentrations show seasonal variations. Shrimp shows an approximately constant concentration of mercury which might testify to the regulation capacity of shrimp for mercury. Crab, and particular fish, show a seasonal variation which may be correlated with the local application of mercury fungicide, rainfall, and the physiology of the organisms. The rapidity with which mercury applied to the land causes increased concentrations in fish is noteworthy.     

巴颜喀拉山高寒草地及其退化的遥感研究─—以巴颜喀拉山达日县段为例

本文应用遥感技术，探讨了巴颜喀拉山北坡－青海省达日县段高寒草地资源及其退化高山草甸草地的成因、分布和判断标志，将研究区划分为5个草地类型和3个退化草地型，重点分析了高山草甸草地退化的主要原因和影象特征．     

Resource use efficiency and economic losses: implications for sustainable rice production in Vietnam

The study aimed to estimate resource use efficiency and economic losses by using stochastic frontier analysis. The data set were collected in An Giang Province of Vietnam through personal interviews with 199 rice farmers. The results revealed that returns to scale are decreasing. Rice farmers had high levels of output-oriented and input-oriented technical efficiency with the means of 91.92 and 85.39 %, respectively. The mean environmental efficiency was 82.03 %. The mean efficiency of normal inputs was 61.20 %. Among the bad inputs, pesticide and energy were the least efficient ones with the mean values of 51.39 and 45.53 %, respectively, indicating serious overuses of these inputs. As regards normal inputs, capital had the lowest efficiency score at 21.08 %, followed by seed quantity at 26.4 %. Further, the total economic losses were estimated at 8261 thousand VND (380 USD) per hectare, which is equal to the sales of about 1600 kg of rice per hectare or the efforts to increase by 20 % of output level. This study suggests that rice farmers need to contract inputs, particularly environmentally detrimental inputs to improve profits. To improve the productive efficiency, the use of ecological engineering, collective pump and cultivation of three rice crops per year are possible options.     

Effects of pyrethroid insecticide seed treatments on Rhizobium japonicum and its symbiotic relationship with,and growth of soybean

Abstract

Two pyrethroid insecticides were compared with two fungicides for their effects on the rhizobial and seedling components of the N₂‐fixing symbiosis in soybeans (Glycine max Merr. variety Olinda). In vitro growth of Rhizobium japonicum RI16, was inhibited in the order of HgCl₂ > thiram > cypermethrin > permethrin. The emergence of the seedlings was stimulated by all chemicals tested. Also, none of the pesticides studied had any significant effect on N₂ fixation (acetylene reduction), at the dosages used. Consequently, yields of the various organs were also not significantly influenced by the chemicals.