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91.
A review is presented on trace gas exchange of CH4, CO, N2O, and NOx arising from agriculture and natural sources in the world's semiarid and arid zones due to soil processes. These gases are important contributors to the radiative forcing and the chemistry of the atmosphere. Quantitative information is summarized from the available studies. Between 5 and 40% of the global soil-atmosphere exchange for these gases (CH4, CO, N2O, and NOx) may occur in semiarid and arid zones, but for each of these gases there are fewer than a dozen studies to support the individual estimates, and these are from a limited number of locations. Significant differences in the biophysical and chemical processes controlling these trace gas exchanges are identified through the comparison of semiarid and arid zones with the moist temperate or wet/dry savanna land regions. Therefore, there is a poorly quantified understanding of the contribution of these regions to the global trace gas cycles and atmospheric chemistry. More importantly, there is a poor understanding of the feedback between these exchanges, global change, and regional land use and air pollution issues. A set of research issues is presented. 相似文献
92.
Kammann U Lang T Berkau AJ Klempt M 《Environmental science and pollution research international》2008,15(7):600-605
BACKGROUND, AIM, AND SCOPE: Gene expression analyses with real-time (RT)-polymerase chain reaction (PCR) gains importance in marine monitoring. This new technique has to be compared to the classical approaches like the well known biomarker ethoxyresorufin-O-deethylase (EROD) to test their suitability for monitoring programmes. The goal of the present study is to compare EROD activity and CYP1A1 mRNA expression in the important monitoring fish species dab (Limanda limanda) and to answer the question of whether these parameters reflect the polycyclic aromatic hydrocarbon (PAH) contamination of the fish. Further on, glyceraldehyd-3-phosphate dehydrogenase (GAPDH) was investigated as a potential housekeeping gene. MATERIALS AND METHODS: Female dab were caught in the summer of 2004 in the North Sea and in the Baltic. EROD activity was determined in liver samples by a kinetic fluorimetric assay according to a standard protocol. The gene expression of CYP1A (cytochrome P450 1A) and GAPDH were determined by means of RT-PCR. Results were compared to gonado somatic index and to the concentration of PAH metabolite 1OHPyr (1-hydroxypyrene) analysed in the bile fluids of the fish, respectively. RESULTS: Dab from all stations showed a considerable individual variation in the levels of both CYP1A mRNA and EROD. Highest mean values for CYP1A mRNA and EROD were detected in the northern part of the sampling area. In contrast, the PAH metabolite 1OHPyr was found at the highest concentration in fish caught near the German coast. CYP1A mRNA and EROD showed only a minor but significant correlation (r = 0.32, p < 0.05, n = 123). 1OHPyr in bile correlated significantly (p < 0.05) with the amount of GAPDH mRNA content in the liver. DISCUSSION: The significant but low correlation of CYP1A mRNA and EROD activity on an individual basis illustrates that these two parameters are apparently not closely linked. However, maximum EROD values correspond with maximum CYP1A mRNA concentrations when station means are regarded. Because EROD and CYP1A mRNA in dab follow different physiological principles, their application will lead to related but not identical monitoring results. This should be taken into account when future marine monitoring programmes are designed. The results also indicate that PAH are not the crucial factor for CYP1A and EROD levels in dab from the off-shore areas in the North Sea. This is remarkable because the PAH metabolism is known to be CYP1A-dependent and the widely used biomarker EROD has been recommended for monitoring PAH-related effects in fish from the North Sea. Due to a correlation between GAPDH and 1OHPyr, GAPDH was not suitable as housekeeping gene for dab. CONCLUSIONS: Neither the results from EROD nor from CYP1A1 mRNA measurements in dab reflected their exposure to PAH as measured by the PAH metabolite 1OHPyr. Thus, the question arises of whether EROD or CYP1A mRNA is a suitable biomarker at all to indicate PAH exposure in dab from the open North Sea. RECOMMENDATIONS AND PERSPECTIVES: For future biological effect monitoring, it is advisable to measure more and predominately independent parameters by RT-PCR and to incorporate more components of the detoxification system. 相似文献
93.
The zebrafish embryo model in environmental risk assessment—applications beyond acute toxicity testing 总被引:4,自引:3,他引:1
Scholz S Fischer S Gündel U Küster E Luckenbach T Voelker D 《Environmental science and pollution research international》2008,15(5):394-404
BACKGROUND, AIM, AND SCOPE: The use of fish embryos is not regulated by current legislations on animal welfare and is therefore considered as a refinement, if not replacement of animal experiments. Fish embryos represent an attractive model for environmental risk assessment of chemicals since they offer the possibility to perform small-scale, high-throughput analyses. MAIN FEATURES: Beyond their application for determining the acute toxicity, fish embryos are also excellent models for studies aimed at the understanding of toxic mechanisms and the indication of possible adverse and long-term effects. Therefore, we have reviewed the scientific literature in order to indicate alternative applications of the fish embryo model with focus on embryos of the zebrafish. RESULTS AND DISCUSSIONS: The analysis of the mode of action is important for the risk assessment of environmental chemicals and can assist in indicating adverse and long-term effects. Toxicogenomics present a promising approach to unravel the potential mechanisms. Therefore, we present examples of the use of zebrafish embryos to study the effect of chemicals on gene and protein patterns, and the potential implications of differential expression for toxicity. The possible application of other methods, such as kinase arrays or metabolomic profiling, is also highlighted. Furthermore, we show examples of toxicokinetic studies (bioconcentration, ABC transporters) and discuss limitations that might be caused by the potential barrier function of the chorion. Finally, we demonstrate that biomarkers of endocrine disruption, immune modulation, genotoxicity or chronic toxicity could be used as indicators or predictors of sub-acute and long-term effects. CONCLUSIONS: The zebrafish embryo represents a model with an impressive range of possible applications in environmental sciences. Particularly, the adaptation of molecular, system-wide approaches from biomedical research is likely to extend its use in ecotoxicology. RECOMMENDATIONS AND PERSPECTIVES: Challenges for future research are (1) the identification of further suitable molecular markers as indicators of the mode of action, (2) the establishment of strong links between (molecular) effects in short-term assays in embryos and long-term (toxic) effects on individuals, (3) the definition of limitations of the model and (4) the development of tests that can be used for regulatory purposes. 相似文献
94.
Efficacy of extracting indices from large‐scale acoustic recordings to monitor biodiversity
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Rachel T. Buxton Mary Clapp Erik Meyer Erik Stabenau Lisa M Angeloni Kevin Crooks George Wittemyer 《Conservation biology》2018,32(5):1174-1184
Passive acoustic monitoring could be a powerful way to assess biodiversity across large spatial and temporal scales. However, extracting meaningful information from recordings can be prohibitively time consuming. Acoustic indices (i.e., a mathematical summary of acoustic energy) offer a relatively rapid method for processing acoustic data and are increasingly used to characterize biological communities. We examined the relationship between acoustic indices and the diversity and abundance of biological sounds in recordings. We reviewed the acoustic‐index literature and found that over 60 indices have been applied to a range of objectives with varying success. We used 36 of the most indicative indices to develop a predictive model of the diversity of animal sounds in recordings. Acoustic data were collected at 43 sites in temperate terrestrial and tropical marine habitats across the continental United States. For terrestrial recordings, random‐forest models with a suite of acoustic indices as covariates predicted Shannon diversity, richness, and total number of biological sounds with high accuracy (R2 ≥ 0.94, mean squared error [MSE] ≤170.2). Among the indices assessed, roughness, acoustic activity, and acoustic richness contributed most to the predictive ability of models. Performance of index models was negatively affected by insect, weather, and anthropogenic sounds. For marine recordings, random‐forest models poorly predicted Shannon diversity, richness, and total number of biological sounds (R2 ≤ 0.40, MSE ≥ 195). Our results suggest that using a combination of relevant acoustic indices in a flexible model can accurately predict the diversity of biological sounds in temperate terrestrial acoustic recordings. Thus, acoustic approaches could be an important contribution to biodiversity monitoring in some habitats. 相似文献
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Assessing the Vulnerability of Coastal Communities to Extreme Storms: The Case of Revere,MA., USA 总被引:6,自引:1,他引:6
Clark George E. Moser Susanne C. Ratick Samuel J. Dow Kirstin Meyer William B. Emani Srinivas Jin Weigen Kasperson Jeanne X. Kasperson Roger E. Schwarz Harry E. 《Mitigation and Adaptation Strategies for Global Change》1998,3(1):59-82
Climate change may affect the frequency, intensity, and geographic distribution of severe coastal storms. Concurrent sea-level
rise would raise the baseline of flooding during such events. Meanwhile, social vulnerability factors such as poverty and
disability hinder the ability to cope with storms and storm damage. While physical changes are likely to remain scientifically
uncertain into the foreseeable future, the ability to mitigate potential impacts from coastal flooding may be fostered by
better understanding the interplay of social and physical factors that produce human vulnerability. This study does so by
integrating the classic causal model of hazards with social, environmental, and spatial dynamics that lead to the differential
ability of people to cope with hazards. It uses Census data, factor analysis, data envelopment analysis, and floodplain maps
to understand the compound social and physical vulnerability of coastal residents in the city of Revere, MA, USA.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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The lack of suitable and appropriate crops has been a poorly recognized obstacle to the application of sound agroecological practices in some temperate and tropical regions. Cultivation of traditional hybrid crops in inherently hostile environments forces increased managerial and economic expenditures. Selection of ecologically harmonious crops exploits, rather than opposes natural forces. Based in part on US-AID funded research, we have identified three crops suitable for temperate/tropical cultivation. Amaranthus is recognized in Asia and Central and South America as a food crop that can be grown as a leafy vegetable or as a seed grain in mixed croppings. It does well under a wide variety of conditions using established cultural methods. It is nutritious and highly palatable and, in many areas, is well recognized in the market place. Prosopis species (mesquite) leguminous trees occur worldwide in arid and semi-arid areas. As phreatophytes, they are resistant to drought and high temperature extremes and produce abundant yields of edible beanlike pods. Using the beans from the tree, a variety of attractive, marketable food products have been prepared. We have also identified intermediate wheat-grass, a perennial relative of wheat, as an ecologically and economically attractive alternate crop. It is already a well-established range crop; grown for seed, it becomes a useful grain intercrop. The wholegrain or endosperm mill fractions make excellent tasting, appetizing and attractive baked products. Ongoing agronomic studies indicate it is especially suitable for balancing ecologically unstable areas. 相似文献