The combined use of SEM-EDX,Raman, ATR-FTIR and visible reflectance techniques for the characterisation of Roman wall painting pigments from Monte d’Oro area (Rome): an insight into red,yellow and pink shades

Environmental Science and Pollution Research - The aim of this work has been the identification of the painter’s materials employed in the wall decoration of some destroyed buildings dating...     

Properties and erosional response of soils in a degraded ecosystem in Crete (Greece)

To study desertification processes relating to soil erosion, a climatological and altitudinal gradient from south to north was selected in Crete (Greece) and four locations were selected along the gradient. At the locations precipitation ranged from 1400 mm/year at the highest location to 400 mm/year at the lowest. All locations are affected by the actual land use: intensive grazing, small controlled fires, and abandoned agricultural terraces. Representative soil profiles were described in the field and analyzed in the laboratory, and rainfall simulation experiments in the field measured soil erosion over different soil surfaces and land uses. Data on physical and chemical properties were obtained from the soil profiles and soil hydrology, and erosion data were obtained from the rainfall simulation experiments. Soil aggregation was studied with samples taken from the soil in the rainfall simulation plots and special attention being paid to the aggregate size distribution and the water-stable microaggregation. The interaction between climatological conditions and land use seems to be the main factor controlling soil erosion. This paper describes how the expected erosion along the gradient (from the most humid to the driest site) can be affected and disturbed by specific processes derived from land use.     

Spatial pattern of ozone injury in Aleppo pine related to air pollution dynamics in a coastal-mountain region of eastern Spain

In eastern Spain, studies combining the tracking and meso-scale circulations of air pollutants with the evaluation of their effects on plants have been undertaken since 1994. Meso-scale processes are very important from the point of view of how and where forest ecosystems are affected by point sources and regional air pollution in the Mediterranean area. The first results of these field surveys show that in 1994, 1995 and 1996, the distribution pattern of ozone visual injury (chlorotic mottle) in Pinus halepensis correlated with the penetration of pollutants transported by the sea-breeze into coastal valleys of Castellón (eastern Spain). In this tree species, longer needles are associated with higher chlorotic mottle, and ozone injury seems to be among the factors affecting needle retention and crown transparency.     

Genotoxicity of gold nanoparticles functionalized with indolicidin towards Saccharomyces cerevisiae

The toxic effects of gold nanoparticles surface-functionalized with the antimicrobial peptide indolicidin(Au NPs-indolicidin) towards the yeast Saccharomyces cerevisiae, one of the major eukaryotic model organisms, have been evaluated. Growth and survival,genotoxicity, as measured by comet assay, and expression of the YCA1, an apoptosis indicating gene, following 72 hr exposure of yeast to Au NPs-indolicidin, and to Au NPs and indolicidin alone have been examined. The gold nanoparticles exerted toxicity with DNA damage, accompanied by reactive oxygen species production(ROS), but they do not inhibit yeast growth and viability. Genotoxicity was less pronounced for surface-functionalized nanoparticles, showing that S. cerevisiae is quite resistant to the complex Au NPs-indolicidin.A progressive reduction of the genotoxic effect was observed along 72 hr exposure,presumably due to the activation of DNA repair mechanisms. These findings suggest the occurrence of a physiological protective response of S. cerevisiae towards nanoparticles,thereby providing useful information to the assessment of the environmental impact of metal nanoparticles.     

Effects of copper on early developmental stages of Lessonia nigrescens Bory (Phaeophyceae)

Copper effects on the early developmental gametophytic and sporophytic stages of the kelp Lessonia nigrescens were tested in gradients of increasing concentrations of ASV-labile copper. The results demonstrated a high sensitivity to copper of all life-history stages of the alga, where even the lowest tested concentration affected spore release as well as their subsequent settlement. More significant, concentrations higher than 7.87 microg L(-1) totally interrupted the development of the spores after they settle. This effect led to a failure in the formation of male and female gametophytes and, as a consequence, to a complete disruption of the normal life cycle of the kelp. Thus, we suggest that the absence of L. nigrescens from copper-enriched environments results from the high sensitivity of its early life cycle stages, which limits growth and maturation of the gametophytic microscopic phase and, as a consequence, prevents development of the macroscopic sporophytic phase.     

Long-term changes in phytoplankton phenology and community structure in the Bahía Blanca Estuary, Argentina

The phytoplankton of the Bahía Blanca Estuary, Argentina, has been surveyed since 1978. Chlorophyll a, phytoplankton abundance, species composition and physico-chemical variables have been fortnightly recorded. From 1978 to 2002, a single winter–early spring diatom bloom has dominated the main pattern of phytoplankton interannual variability. Such pattern showed noticeable changes since 2006: the absence of the typical winter bloom and changes in phenology, together with the replacement of the dominant blooming species, i.e. Thalassiosira curviseriata, and the appearance of different blooming species, i.e. Cyclotella sp. and Thalassiosira minima. The new pattern showed relatively short-lived diatom blooms that spread throughout the year. In addition, shifts in the phytoplankton size structure toward small-sized diatoms, including the replacement of relatively large Thalassiosira spp. by small Cyclotella species and Chaetoceros species have been noticed. The changes in the phenology and composition of the phytoplankton are mainly attributed to warmer winters and the extremely dry weather conditions evidenced in recent years in the Bahía Blanca area. Changing climate has modified the hydrological features in the inner part of the estuary (i.e. higher temperatures and salinities) and potentially triggered the reorganization of the phytoplankton community. This long-term study provides evidence on species-specific and structural changes at the bottom of the pelagic food web likely related to the recent hydroclimatic conditions in a temperature estuary of the southwestern Atlantic.     

Instrumental and bio-monitoring of heavy metal and nanoparticle emissions from diesel engine exhaust in controlled environment

In the present article we characterized the emissions at the exhaust of a Common Rail (CR) diesel engine, representative of lightduty class, equipped with a catalyzed diesel particulate filter (CDPF) in controlled environment. The downstream exhausts were directly analyzed (for PM, CO, CO2, O2, HCs, NOx) by infrared and electrochemical sensors, and SEM-EDS microscope; heavy metals were chemically analyzed using mosses and lichens in bags, and glass-fibre filters all exposed at the engine exhausts. The highest particle emission value was in the 7–54 nm size range; the peak concentration rose until one order of magnitude for the highest load and speed. Particle composition was mainly carbonaceous, associated to noticeable amounts of Fe and silica fibres. Moreover, the content of Cu, Fe, Na, Ni and Zn in both moss and lichen, and of Al and Cr in moss, was significantly increased. Glass-fibre filters were significantly enriched in Al, B, Ba, Cu, Fe, Na, and Zn. The role of diesel engines as source of carbonaceous nanoparticles has been confirmed, while further investigations in controlled environment are needed to test the catalytic muffler as a possible source of silica fibres considered very hazardous for human health.     

Bioremediation of aquaculture wastewater from Mugil cephalus (Linnaeus, 1758) with different microalgae species

Current aquaculture practices have a detrimental impact on the environment, in particular due to the release of high concentration of nitrogen and phosphorus that can induce eutrophication. This study investigates and compares the capacity of three microalgae species Tetraselmis suecica, Isochrysis galbana and Dunaliella tertiolecta, in the bioremediation of grey mullet Mugil cephalus wastewater. The experiment was conducted in batch conditions for 7 days using completely mixed bubble column photobioreactors. After two days, T. suecica and D. tertiolecta were able to remove more than 90% of dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorous (DIP), whereas I. galbana removed only 32% and 79% of DIN and DIP, respectively. A higher biomass yield resulted for T. suecica (603?±?34?mg/L, mean?±?SE). This study confirms the potential to employ T. suecica in an Integrated Multi-Trophic Aquaculture system for bioremediation of wastewater and identifies D. tertiolecta as another valid candidate species. Moreover, these species can growth in unsterilized culture media, and this reduces energy consumption, costs and efforts.     

Mapping and quantifying habitat fragmentation in small coastal areas: a case study of three protected wetlands in Apulia (Italy)

In the Mediterranean Region, habitat loss and fragmentation severely affect coastal wetlands, due to the rapid expansion of anthropogenic activities that has occurred in the last decades. Landscape metrics are commonly used to define landscape patterns and to evaluate fragmentation processes. This investigation focuses on the performance of a set of landscape pattern indices within landscapes characterized by coastal environments and extent below 1,000 ha. The aim is to assess the degree of habitat fragmentation for the monitoring of protected areas and to learn whether values of landscape metrics can characterize fine-resolution landscape patterns. The study areas are three coastal wetlands belonging to the Natura 2000 network and sited on the Adriatic side of Apulia (Southern Italy). The Habitat Maps were derived from the Vegetation Maps generated integrating phytosociological relevés and Earth Observation data. In the three sites, a total of 16 habitat types were detected. A selected set of landscape metrics was applied in order to investigate their performance in assessing fragmentation and spatial patterns of habitats. The final results showed that the most significant landscape patterns are related to highly specialized habitat types closely linked to coastal environments. In interpreting the landscape patterns of these highly specialized habitats, some specific ecological factors were taken into account. The shape indices were the most useful in assessing the degree of fragmentation of habitat types that usually have elongated morphology along the shoreline or the coastal lagoons. In all the cases, to be meaningful, data obtained from the application of the selected indices were jointly assessed, especially at the class level.