Non-invasive measurement of carbon monoxide burden in Guatemalan children and adults following wood-fired temazcal (sauna-bath) use

The use of wood-fired steam baths, or temazcales, is a potentially dangerous source of CO exposure in Guatemalan Highland communities where adults and children use them regularly for bathing, relaxation, and healing purposes. Physical characteristics of children predispose them to absorb CO faster than adults, placing them at greater exposure and health risks. Efforts to quantify temazcal exposures across all age groups, however, have been hampered by the limitations in exposure measurement methods. In this pilot study we measured COHb levels in children and adults following use of the temazcal using three field-based, non-invasive CO measurement methods: CO-oximetry, exhaled breath, and by estimation of COHb using micro-environmental concentrations and time diaries. We then performed a brief comparison of methods. Average CO concentrations measured during temazcal use were 661 ± 503 ppm, approximately 10 times the 15 min WHO guideline. Average COHb levels for all participants ranged from 12-14% (max of 30%, min 2%), depending on the method. COHb levels measured in children were not significantly different from adults despite the fact that they spent 66% less time exposed. COHb measured by CO-oximetry and exhaled breath had good agreement, but precision of the former was affected substantially by random instrument error. The version of the field CO-oximeter device used in this pilot could be useful in screening for acute CO exposure events in children but may lack the precision for monitoring the burden from less extreme, but more day-to-day CO exposures (e.g. indoor solid fuel use). In urban settings, health effects in children and adults have been associated with chronic exposure to ambient CO concentrations much lower than measured in this study. Future research should focus on reducing exposure from temazcales through culturally appropriate modifications to their design and practices, and targeted efforts to educate communities on the health risks they pose and actions they can take to reduce this risk.     

Hg bioavailability and impact on bacterial communities in a long-term polluted soil

Different soil samples characterised by a long-term Hg-pollution were studied for Hg total content, fractionation, phytotoxicity and influence on the bacterial community. Hg pollution ranged from 1 to 50 mg kg(-1) and most of it was speciated in scarcely soluble forms. In agreement with this, the biochemical quality indexes were investigated (biomass, enzyme activities) and the bacterial community (viable heterotrophic (VH) bacteria, functional diversity) apparently was not influenced by the degree of Hg pollution. In particular, the investigated soils exhibited a low percentage of Hg-resistant (Hg(R)) bacteria ranging from less than 0.001% to 0.25% of the VH and the addition of available Hg in the form of HgCl(2) induced an enrichment of resistant Hg(R) populations. The general biodiversity of the bacterial community was evaluated by denaturing gradient gel electrophoresis of DNA of Hg spiked soil microcosms and of control soils. Hg(R) bacteria capable to grow in a minimal medium containing HgCl(2) were also isolated and identified. MerA and merB gene PCR fragments were obtained from different Hg(R) strains and the range of similarities at the DNA level and at the deduced amino acid level showed that they carried mercuric reductase and lyase. Differently from bacteria, some influence of soil Hg content on seeds' germination and root elongation was observed for Lepidium sativum L. and Solanum lycopersicum L. In conclusion, most of the Hg in these long-term polluted soils was scarcely mobile and available and did not significantly influence the soil bacterial community. The risk of potential Hg remobilization over time, that could be naturally favoured by the activity of plant roots or other inorganic processes occurring in soil, can be extenuated since bacterial community was resistant and resilient to subsequent Hg stress.     

The validation analysis of the INSHORE system��a precise and efficient coastal survey system

Government and environmental entities are becoming increasingly concerned with qualifying and quantifying the erosion effects that are observed in sandy shores. Correspondingly, survey methodologies that gather data for such erosion studies are increasingly being demanded. The responsible entities are continually broadening their areas of interest, are concerned in the establishment of regular monitoring programmes and are demanding high accuracy from the geo-spatial data that is collected. The budget available for such monitoring activities, however, does not parallel the trend in the increasing demand for quality specifications. Survey methodologies need improvement to meet these requirements. We have developed a new land-based survey system--the INSHORE system--that is ideal for low cost, highly efficient and highly precise coastal surveys. The INSHORE system uses hi-tech hardware that is based on high-grade global positioning system (GPS) receivers and a laser distance sensor combined with advanced software algorithms. This system enables the determination of the ground coordinates of the surveyed areas with a precision of 1 to 2 cm, without having a sensor in contact with the ground surface. The absence of physical contact with the ground makes this system suitable for high-efficiency surveys. The accuracy of the positioning, which is based on advanced differential GPS processing, is enhanced by considering the estimated attitude of the GPS receiver holding structure and eliminates undesirable offsets. This paper describes the INSHORE survey system and presents the results of validation tests that were performed in a sandy shore environment.     

Heavy metal pollution in water and sediments in the Kabini River, Karnataka, India

Critical to habitat management is the understanding of not only the location of animal food resources, but also the timing of their availability. Grizzly bear (Ursus arctos) diets, for example, shift seasonally as different vegetation species enter key phenological phases. In this paper, we describe the use of a network of seven ground-based digital camera systems to monitor understorey and overstorey vegetation within species-specific regions of interest. Established across an elevation gradient in western Alberta, Canada, the cameras collected true-colour (RGB) images daily from 13 April 2009 to 27 October 2009. Fourth-order polynomials were fit to an RGB-derived index, which was then compared to field-based observations of phenological phases. Using linear regression to statistically relate the camera and field data, results indicated that 61% (r (2)?= 0.61, df = 1, F?= 14.3, p?= 0.0043) of the variance observed in the field phenological phase data is captured by the cameras for the start of the growing season and 72% (r (2)?= 0.72, df = 1, F?= 23.09, p?= 0.0009) of the variance in length of growing season. Based on the linear regression models, the mean absolute differences in residuals between predicted and observed start of growing season and length of growing season were 4 and 6?days, respectively. This work extends upon previous research by demonstrating that specific understorey and overstorey species can be targeted for phenological monitoring in a forested environment, using readily available digital camera technology and RGB-based vegetation indices.     

Correlation of six anthropogenic markers in wastewater, surface water, bank filtrate, and soil aquifer treatment

Six trace contaminants (acesulfame (ACE), sucralose (SUC), carbamazepine (CBZ), diatrizoic acid (DTA), 1H-benzotriazole (BTZ) and its 4-methyl analogue (4-TTri)) were traced from wastewater treatment plants (WWTPs) to receiving waters and further to riverbank filtration (RBF) wells to evaluate their prediction power as potential wastewater markers. Furthermore, the persistence of some compounds was investigated in advanced wastewater treatment by soil aquifer treatment (SAT). During wastewater treatment in four conventional activated sludge WWTPs ACE, SUC, and CBZ showed a pronounced stability expressed by stable concentration ratios in influent (in) and effluent (out) (ACE/CBZ: in45, out40; SUC/CBZ: in1.8, out1.7; and ACE/SUC: in24, out24). In a fifth WWTP, additional treatment with powdered activated carbon led to a strong elimination of CBZ, BTZ, and 4-TTri of about 80% and consequently to a distinctive shift of their ratios with unaffected compounds. Data from a seven month monitoring program at seven sampling locations at the rivers Rhine and Main in Germany revealed the best concentration correlation for ACE and CBZ (r(2) = 0.94) and also a good correlation of ACE and CBZ concentrations to BTZ and 4-TTri levels (r(2) = 0.66 to 0.82). The comparison of ratios at different sampling sites allowed for the identification of a CBZ point source. Furthermore, in Switzerland a higher consumption of SUC compared to Germany can be assumed, as a steadily increasing ACE/SUC ratio along the river Rhine was observed. In RBF wells a good correlation (r(2) = 0.85) was again observed for ACE and CBZ. Both also showed the highest stability at a prolonged residence time in the subsurface of a SAT field. In the most peripheral wells ACE and CBZ were still detected with mean values higher than 36 μg L(-1) and 1.3 μg L(-1), respectively. Although SUC concentrations in wastewater used for SAT decreased by more than 80% from about 18 μg L(-1) to 2.1 μg L(-1) and 3.5 μg L(-1) in these outlying wells, the compound was still adequate to indicate a wastewater impact in a qualitative way.     

Impact assessment of subsurface drainage on waterlogged and saline lands

Waterlogging and salinity due to seepage from canals have polluted land and environment in various parts of Pakistan. A sustainable environment requires urgent remedial measures for this problem. The research in this paper presents the impacts of the Fourth Drainage Project, Faisalabad on the twin problem of waterlogging and salinity. Monitoring of the project was made on regular basis. The key performance indicators for the project include the lowering of water table, improvement of water quality and soil salinity, increase in area under cultivation, cropping intensity, and socioeconomic status of the project population. Data regarding water levels and discharge from the drain pipes were collected to monitor the impact on waterlogging. Soil samples were tested to evaluate the impact of drainage on land. It has been found that the percentage of the contaminated land in the project area has considerably been decreased, while the cropping intensities have been increased.     

Chemometrics in assessment of seasonal variation of water quality in fresh water systems

The spatial and temporal distribution of polycyclic aromatic hydrocarbons (PAHs) was investigated in Gomti River, a major tributary of the Ganga river (India). A total of 96 samples (water and sediments) were collected from eight different sites over a period of 2 years and analysed for 16 PAHs. The total concentrations of 16 PAHs in water and bed sediments ranged between 0.06 and 84.21 ??g/L (average (n?=?48), 10.33 ± 19.94 ??g/L) and 5.24?C3,722.87 ng/g dw [average (n?=?48): 697.25 ± 1,005.23 ng/g dw], respectively. In water, two- and three-ring PAHs and, in sediments, the three- and four-ring PAHs were the dominant species. The ratios of anthracene (An)/An + phenenthrene and fluoranthene (Fla)/Fla + pyrene were calculated to evaluate the possible sources of PAHs. These ratios reflected a pattern of pyrolytic input as a major source of PAHs in the river. Principal component analysis, further, separated the PAHs sources in the river sediments, suggesting that both the pyrolytic and petrogenic sources are contributing to the PAHs burden. The threat to biota of the river due to PAHs contamination was assessed using effect range low and effect range median values, and the results suggested that sediment at some occasions may pose biological impairment.     

Peptidchemie heute

Peptide chemistry has reached a plateau on which more work is devoted to the improvement of known basic principles than to the development of really new methods. As a consequence, routine syntheses of peptides concentrate on few protecting groups and coupling procedures only. The recent progress in purification of peptides by liquid chromatography helped to promote this trend. Meanwhile, recombinant technology permits the synthesis of proteins and possibly of some lower peptides, too. It cannot compete, however, with chemical solid-phase synthesis of a newly discovered peptide with up to about 40 amino acids in speed but may take over the synthesis for large-scale production. Peptides containing modified or non-natural structures, which are often superior to the natural compounds as drugs, will remain subject of chemical synthesis, too. Present research focuses on the role of peptide and protein factors in the immune response and on structure and biological activities of neuropeptides.