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491.
Although Dutch cities were among the forerunners in local climate policy, a systematic overview on climate mitigation and adaptation policy is still missing. This study aims to fill this gap by analysing 25 Dutch cities using indicators for the level of anchoring in policy, organisation and practical implementation as well as multi-level relations. Since Tilburg, Amsterdam, Den Haag and Rotterdam show a higher performance than other Dutch cities, these four cities are used as reference cities. The findings suggest that structural integration of climate mitigation and adaptation is limited in Dutch cities. The study points at three recent trends in local climate governance in the Netherlands: (i) decentralisation within municipal organisations, (ii) externalisation initiatives that place climate policy outside the municipal organisation and (iii) regionalisation with neighbouring municipalities and the provincial government. 相似文献
492.
Anne-Leila Meistertzheim Isabelle Calvès Valérie Roussel Alain Van Wormhoudt Jean Laroche Sylvain Huchette Christine Paillard 《Marine Biology》2014,161(8):1883-1893
Increasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. Numerous episodic mass mortalities of the abalone Haliotis tuberculata have been observed along the northern Brittany coast of France caused by a complex interaction between the host, pathogen and environmental factors. Here, we evaluated the potential of high-resolution melting (HRM) analysis for mutation genotyping and development of genetic markers for resistance to vibriosis in the gastropod species H. tuberculata. Small amplicon assays were developed and revealed genetic polymorphism between surviving and susceptible abalone obtained after two successive infections of aquaculture families in controlled conditions. Together with specific COI haplotypes, we identified particular genotypes in nascent polypeptide-associated complex subunit alpha and ferritin genes linked to the susceptibility or resistance of abalone to vibriosis. Selection of genitors based on these genes may increase the proportion in offspring of resistant individuals of more than 76 %. Finally, HRM assays constitute a very efficient genotyping tool to validate the genetic markers on a representative number of individuals of wild populations and thus identify future resistant genitors for aquaculture or conservation purposes. 相似文献
493.
Critically evaluating best management practices for preventing freshwater turtle extinctions
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Ex situ conservation tools, such as captive breeding for reintroduction, are considered a last resort to recover threatened or endangered species, but they may also help reduce anthropogenic threats where it is difficult or impossible to address them directly. Headstarting, or captive rearing of eggs or neonate animals for subsequent release into the wild, is controversial because it treats only a symptom of a larger conservation problem; however, it may provide a mechanism to address multiple threats, particularly near population centers. We conducted a population viability analysis of Australia's most widespread freshwater turtle, Chelodina longicollis, to determine the effect of adult roadkill (death by collision with motor vehicles), which is increasing, and reduced recruitment through nest predation from introduced European red foxes (Vulpes vulpes). We also modeled management scenarios to test the effectiveness of headstarting, fox management, and measures to reduce mortality on roads. Only scenarios with headstarting from source populations eliminated all risks of extinction and allowed population growth. Small increases in adult mortality (2%) had the greatest effect on population growth and extinction risk. Where threats simultaneously affected other life‐history stages (e.g., recruitment), eliminating harvest pressures on adult females alone did not eliminate the risk of population extinction. In our models, one source population could supply enough hatchlings annually to supplement 25 other similar‐sized populations such that extinction was avoided. Based on our results, we believe headstarting should be a primary tool for managing freshwater turtles for which threats affect multiple life‐history stages. We advocate the creation of source populations for managing freshwater turtles that are greatly threatened at multiple life‐history stages, such as depredation of eggs by invasive species and adult mortality via roadkill. 相似文献
494.
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496.
Ngo Thanh Huy Van Dieu-Anh Tran Hoai Le Nakada Norihide Tanaka Hiroaki Huynh Trung Hai 《Environmental science and pollution research international》2021,28(10):12082-12091
Environmental Science and Pollution Research - This study evaluated the occurrence of PPCPs in Cau River (Vietnam). Surface water and sediment samples were collected to determine PPCP... 相似文献
497.
Brigitte H. W. Faas Dineke Westra Sonja A. de Munnik Maartje van Rij Carlo Marcelis Sara Joosten Ingrid Krapels Vivian Vernimmen Malou Heijligers Marjolein H. Willemsen Nicole de Leeuw Tuula Rinne Rolph Pfundt Sanne P. Smeekens Sander P. A. Stegmann Merryn Macville Esther Sikkel Audrey Coumans Lia Wijnberger Irma Derks Josefa van Lent-Albrechts Tom Hofste Raoul Timmermans Janneke van den End Servi J. C. Stevens Ilse Feenstra 《黑龙江环境通报》2023,43(4):527-543
Objective
We performed a 1-year evaluation of a novel strategy of simultaneously analyzing single nucleotide variants (SNVs), copy number variants (CNVs) and copy-number-neutral Absence-of-Heterozygosity from Whole Exome Sequencing (WES) data for prenatal diagnosis of fetuses with ultrasound (US) anomalies and a non-causative QF-PCR result.Methods
After invasive diagnostics, whole exome parent-offspring trio-sequencing with exome-wide CNV analysis was performed in pregnancies with fetal US anomalies and a non-causative QF-PCR result (WES-CNV). On request, additional SNV-analysis, restricted to (the) requested gene panel(s) only (with the option of whole exome SNV-analysis afterward) was performed simultaneously (WES-CNV/SNV) or as rapid SNV-re-analysis, following a normal CNV analysis.Results
In total, 415 prenatal samples were included. Following a non-causative QF-PCR result, WES-CNV analysis was initially requested for 74.3% of the chorionic villus (CV) samples and 45% of the amniotic fluid (AF) samples. In case WES-CNV analysis did not reveal a causative aberration, SNV-re-analysis was requested in 41.7% of the CV samples and 17.5% of the AF samples. All initial analyses could be finished within 2 weeks after sampling. For SNV-re-analysis during pregnancy, turn-around-times (TATs) varied between one and 8 days.Conclusion
We show a highly efficient all-in-one WES-based strategy, with short TATs, and the option of rapid SNV-re-analysis after a normal CNV result. 相似文献498.
Prenatal trio exome sequencing (ES) has become integrated into the care for pregnant women when the fetus has structural anomalies. Details regarding optimizing indications for prenatal exome sequencing, its detection rates with different categories of fetal anomalies, and principles of interpretation of pathogenicity of sequence variants are still under investigation. However, there is now growing consensus about its benefits for finding the cause of fetal structural anomalies. What is not established, is whether exome or genome sequencing (GS) has a place in the care of all pregnant women. This report is a summary of the debate on this topic at the 26th International Conference on Prenatal Diagnosis and Therapy. Both expert debaters considered the advantages and disadvantages. Advantages include the ability to diagnose serious childhood conditions without a prenatally observable phenotype, which creates the potential of early treatments. Disadvantages include difficulties with variant classification, counseling complexities, healthcare cost, and the burden on healthcare systems and families, in particular with the discovery of adult-onset disorders or variants of uncertain significance. Although both debaters weighed the balance of these conflicting arguments differently, they agreed that more research is needed to further explore the clinical utility and ethical aspects of GS for all pregnant women. 相似文献
499.
Agricultural development has been an effective instrument for poverty alleviation and economic development in developing countries over the latter half of the twentieth century, and over 80 % of rural people globally still depend on agriculture for their living. However, issues such as water availability, land degradation and an increasing dependence on chemical fertilisers and pesticides continue to be on-going threats to sustainable agricultural development. These threats are being driven by the pressing need to ensure food security in the face of rapidly growing and urbanising populations. Developing countries will therefore continue to need improved methods for planning sustainable agricultural development. This paper presents a review of agricultural sustainability assessment in developing countries. The review highlights some of the key weaknesses that persist in sustainability assessment and the need to consider not only indicators of sustainability but also the drivers that influence indicator behaviour. We argue that without a good understanding of the drivers of sustainability and their systemic relationships to indicators, sustainability assessments run the risk of focusing on symptoms without addressing underlying causes of adverse indicator trends. Drivers of agricultural sustainability in developing countries encompass a range of demographic, natural, socio-economic, political, institutional and management factors. Understanding these and their relationships to sustainability indicators is needed in order to develop agricultural development policy that supports sustainability. The paper presents a conceptual framework for guiding systemic agricultural sustainability assessment and agricultural development planning in developing countries that includes both sustainability indicators and drives, and considers the broad relationships between them. 相似文献
500.
The promotion of development projects based on the commercialization of non‐timber forest products has increased in recent decades, showing a positive contribution to rural development; yet it has led to controversial approaches in resource preservation. This paper examines the case of Candelilla wax from the Chihuahuan Desert in northern Mexico, identifying its potential contribution to poverty alleviation in marginal areas, and its unique opportunity to access potential markets of a wide variety of industries all around the world. In this paper, the authors base their analysis on three main aspects: social, economic and environmental. Potential benefits that could be obtained from the collaboration of private institutions, development organizations, policymakers and rural producers through integrated rural development projects are featured. 相似文献