Diversity of free-living marine nematodes (Enoplida) from Baja California assessed by integrative taxonomy

We used morphological and molecular approaches to evaluate the diversity of free-living marine nematodes (order Enoplida) at four coastal sites in the Gulf of California and three on the Pacific coast of Baja California, Mexico. We identified 22 morphological species belonging to six families, of which Thoracostomopsidae and Oncholaimidae were the most diverse. The genus Mesacanthion (Thoracostomopsidae) was the most widespread and diverse. Five allopatric species, genetically and morphologically differentiated, were found in two localities in the Gulf of California (M. sp1 and M. sp2) and three in the Pacific coast (M. sp3, M. sp4 and M. sp5). Overall, we produced 19 and 20 sequences for the 18S and 28S genes, respectively. Neither gene displayed intraspecific polymorphisms, which allowed us to establish that some morphological variation was likely either ontogenetic or due to phenotypic plasticity. Although 18S and 28S phylogenies were topologically congruent (incongruence length difference test, P > 0.05), divergences between species were much higher in the 28S gene. Moreover, this gene possessed a stronger phylogenetic signal to resolve relationships involving Rhabdodemania and Bathylaimus. On the other hand, the close relationship of Pareurystomina (Enchilidiidae) with oncholaimids warrants further study. The 28S sequences (D2D3 domain) may be better suited for DNA barcoding of marine nematodes than those from the 18S rDNA, particularly for differentiating closely related or cryptic species. Finally, our results underline the relevance of adopting an integrative approach encompassing morphological and molecular analyses to improve the assessment of marine nematode diversity and advance their taxonomy.     

Prenatal foetal diagnosis of partial trisomy 3q and monosomy 13p due to a maternal balanced rearrangement

The authors describe a case of a male foetus whose ultrasound at 20 weeks' gestation revealed cystic hygroma, cleft lip and ventricular septal defect. Amniotic fluid cytogenetics using GTG banding showed a 46,XY,der(13)t(3;13)(q12;p11.1) rearrangement, and fluorescence in situ hybridization (FISH) delineated the relevant breakpoints. Familial studies identified a maternal balanced translocation involving chromosomes 3 and 13. The post-mortem examination confirmed the prenatal ultrasound findings. Copyright © 2005 John Wiley & Sons, Ltd.     

Novel Whey Protein Isolate/Polyvinyl Biocomposite for Packaging: Improvement of Mechanical and Water Barrier Properties by Incorporation of Nano-silica

Journal of Polymers and the Environment - The current global proposal for withdrawing polymers with high resistance to degradation and from fossil sources from disposable appliances, as well as the...     

In Vitro Degradation and Cytotoxicity Response of Biobased Nanoparticles Prepared by Thiol-ene Polymerization in Miniemulsion

Journal of Polymers and the Environment - Biodegradability is a key feature for the application of polymeric devices in medicine. This study reports an experimental and theoretical study of the...     

Optimization of fuel briquette production from cassava peels,plantain peels and corn cobs

Journal of Material Cycles and Waste Management - Mechanical properties enable to handle storage and transport of fuel briquette. Binder is key element of these mechanical properties. Biobinder is...     

Short- and long-range cues used by ground-dwelling parasitoids to find their host

Parasitoids of phytophagous insects face a detectability–reliability dilemma when foraging for hosts. Plant-related cues are easily detectable, but do not guarantee the presence of the host. Host-related cues are very reliable, but much harder to detect from a distance. Little is known in particular about the way coleopteran parasitoid females use these cues when foraging for a suitable place to lay their eggs. The question is of interest because, unlike hymenopteran larvae, coleopteran parasitoid larvae are highly mobile and able to forage for hosts on their own. We assessed whether females of the parasitoid rove beetle Aleochara bipustulata (L.) (Coleoptera: Staphylinidae) are attracted to plant (Swede roots, Brassica napus) and host-related cues [pupae of the cabbage root fly Delia radicum (L.) (Diptera: Anthomyiidae)]. In the field, A. bipustulata adult females were captured in selective pitfall traps containing pieces of roots damaged by D. radicum larvae, but not in traps containing pieces of healthy roots or D. radicum pupae. However, in the laboratory, the odour of D. radicum pupae attracted A. bipustulata females to mini-pitfalls. Video monitoring in the laboratory showed that foraging A. bipustulata females preferred a zone containing D. radicum pupae and larval tracks rather than one containing an extract of D. radicum-infested roots. Our results suggest a behavioural sequence where A. bipustulata females use plant-related cues at a distance, but then switch their preference to host-related cues at a close range. This would be the first observation of this behaviour in coleopteran parasitoids.     

Comparison of the toxicogenetic potential of sewage sludges from different treatment processes focusing agricultural use

Environmental Science and Pollution Research - A problem that has been dragging in recent decades is the final disposal of the waste produced in the wastewater treatment process. In addition to its...     

Metal mixtures in pregnant women and umbilical cord blood at urban populations—Rio de Janeiro,Brazil

Environmental Science and Pollution Research - This study aims to assess interrelationships between serum lead (Pb), cadmium (Cd), arsenic (As), and mercury (Hg) concentrations in pregnant women in...     

Schooling decisions in guppies (Poecilia reticulata) are based on familiarity rather than kin recognition by phenotype matching

Evidence from a number of freshwater species indicates that fish prefer to school with familiar individuals. Do they also choose to associate with kin? Our experiment tested this idea using the Trinidadian guppy, Poecilia reticulata, a species whose reproductive biology favours the association of kin groups. Juveniles reared together were able to recognise one another on the basis of either visual or chemical cues, but showed no preference for schooling with unfamiliar kin. We therefore conclude that any naturally occurring kin groups in this species will occur as a result of familiarity rather than as a consequence of kin recognition based on phenotype matching.