Molecular phylogeny and population structure of tideland snails in the genus Cerithidea around Japan

Phylogenetic relationships and genetic population structures were analyzed for tideland gastropods in the genus Cerithidea around Japan on the basis of partial sequence of the mitochondrial COI gene. Large genetic divergence was shown between individuals of Cerithidea cingulata in the southern Ryukyus and those in the central Ryukyus and the Japanese Islands. Haplotypes of C. cingulata from the Japanese Islands were paraphyletic with the exclusion of a monophyletic group from the central Ryukyus. Genetic differentiation of C. cingulata was also detected between Amami-Oshima Island and Okinawajima Island. No genetic divergence was found between Cerithidea rhizophorarum in the Japanese Islands and its subspecies C. rhizophorarum morchii in the Ryukyu Islands. The lack of genetic divergence of Cerithidea largillierti between continental China and Japan suggests relatively recent migration between the Japanese Islands and the Asian continent. For all three Cerithidea species distributed in both the Japanese Islands and the Ryukyu Islands, the Tokara Gap and the Kerama Gap were shown to have acted as barriers to the dispersal.     

Coincidence and Upgrade： A Typical Case Study of Rural Ecotourism Development

At present, internal rural tourism is at the stage of upgrading and renewing, and ecotourism has been considered to be the main direction. This paper discussed the concepts and criteria of rural tourism and ecotourism, analyzed a typical case of Nongke Village of Chengdu City-being considered to be the first rural tourism site in China, went deep into the problems of the contents and criteria of the development of rural ecotourism, and tried to probe into the principles of the development of rural ecotourism both theoretically and practically so as to understand the rules of the development of rural ecotourism .     

Rapid growth of clones of the red alga Chondrus crispus: applications in assays of toxic substances and in physiological studies

Growth rates of excised apical segments from three Chondrus crispus Stackhouse clones were rapid, reproducible and easily measured using simple equipment. Clonal segments exhibited a high degree of phenotypic stability, with coefficients of variation in growth rates being typically less than 5%. Sensitivity of the assay was demonstrated using 24-h pulses of the toxicants Cu²⁺ (10 to 150 ppb) and the molluscicide Bayluscide (100 to 500 ppb). As a further demonstration of applicability of this assay system, growth rates were measured over two consecutive 24-h photoperiods using a normally pigmented red gametophytic clone and a green colored mutant derived from it. Growth rates were highest in the first hours of the light phase for both clones, with growth of the green mutant being consistently inferior to that of the normally pigmented one. Significant changes were measured with intervals as short as 4 h using only five apical segments for each treatment. The C. crispus assay described is sensitive, relatively rapid, and statistically robust when applied to toxicant testing in seawater and to physiological studies.     

Distribution of diatoms in the surficial sediments of the Mangyung-Dongjin tidal flat,west coast of Korea (Eastern Yellow Sea)

The relationship between the distribution of benthic diatoms and sediment characteristics of the Mangyung-Dongjin tidal flat on the west coast of Korea was investigated during June and July 1988. Diatoms were collected from the upper 5 mm of sediments at 60 sites along eight transect lines running perpendicular to the shore line. Of the 371 taxa encountered in the study area, 88% were pennate diatoms. Genera represented by the greatest number of species were Navicula, Nitzschia, Amphora, Cocconeis, Fragilaria and Achnanthes. The most abundant species were Paralia sulcata, Navicula sp. #1, N. arenaria and Cymatosira belgica; all were broadly distributed across the tidal flats. The 60 sites could be assigned to eight clusters with respect to similarity in species composition. Discriminant analysis showed that separation cluster was primarily related to the mean grain size of the sediment. The species could not be separated into groups based on similarities in occurrence; a high degree of spatial overlap was observed. The preferences of the more abundant species for grain size were, therefore, analysed by plotting numerical abundance against mean grain size. There were at least four patterns: species groups could be associated with finegrained sediments, those of intermediate size and coarser sediments and the last group showed no discernible pattern.     

ARSENIC CONSUMPTION AND HEALTH RISK PERCEPTIONS IN A RURAL WESTERN U.S. AREA1

ABSTRACT: Churchill County, Nevada, has approximately 23,000 residents, among whom an estimated 13,500 relied on private wells for water supply in 2002. This study examined exposure to arsenic in water supplies among residents with private domestic wells and factors related to householder choice to consume tap water. It compared opinions and concerns about water quality with consumption habits and observed concentrations from tap water samples. The results from 351 households indicated that a majority (75 percent) of respondents consumed tap water and that a minority (38 percent) applied treatment. Approximately 66 percent of those who consumed tap water were exposed to concentrations of arsenic that exceeded 10 ppb. Water consumption was related to application of treatment. Among 98 respondents who were not at all concerned about the health effects of aqueous arsenic, 59 (60 percent) reported consuming tap water with concentrations of arsenic exceeding 10 ppb. Conversely, among 86 respondents who were highly concerned about arsenic, 33 (37 percent) consumed tap water with concentrations of arsenic exceeding 10 ppb. Results from a national sampling effort showed that 620 of 5,304 private wells sampled (11.7 percent) had arsenic concentrations above 10 ppb. The paradox of awareness of arsenic in water supplies coupled with consumption of aqueous arsenic in concentrations greater than 10 ppb may be common in other parts of the nation. Enhanced educational efforts, especially related to tap water sampling and explanations of efficacy of available treatment, may be useful means of reducing exposure through private water supplies.     

Seasonal fluctuations in sand temperature: effects on the incubation period and mortality of loggerhead sea turtle (<Emphasis Type="Italic">Caretta</Emphasis><Emphasis Type="Italic">caretta</Emphasis>) pre-emergent hatchlings in Minabe,Japan

Incubation period, hatching success, and emergence percentage in loggerhead (Caretta caretta) nests were quantified during the 1993 and 1995 nesting seasons and following incubation seasons in Minabe, Wakayama, Japan. Sand and nest temperatures were also monitored. Over the seasons, daily mean sand temperature at nest depth fluctuated between 18.0°C and 33.3°C, with a steep increase in the second week of July and a peak in late August. Temperatures inside the nest chambers were a few degrees above those of the surrounding sand at the end of incubation. The incubation period ranged from 46 to 82 days. A significant negative correlation was found between mean sand temperature and incubation period. The relationship conformed to the day-degree concept. There was no significant seasonal trend in hatching success, but many pre-emergent hatchlings were found dead in most of the clutches during the warmest part of the season. Emergence percentage was correlated with mean sand temperature calculated for 4 days before emergence, suggesting that mortality may be due to heat. This heat-related mortality is considered to be a common phenomenon at our study site, because the peak in emergences coincides with the peak in high temperatures. These temperature effects on hatchling mortality must be taken into account in estimates of hatchling sex ratios. Because sand temperatures already exceed the optimal thermal range for incubation, this population is vulnerable to even small temperature increases resulting from global warming.     

Prenatal diagnosis of partial monosomy 18p(18p11.2→pter) and trisomy 21q(21q22.3→qter) with alobar holoprosencephaly and premaxillary agenesis

A prenatal diagnosis of partial monosomy 18p(18p11.2→pter) and trisomy 21q(21q22.3→qter) in a fetus with alobar holoprosencephaly (HPE) and premaxillary agenesis (PMA) but without the classical Down syndrome phenotype is reported. A 27-year-old primigravida woman was referred for genetic counselling at 21 weeks' gestation due to sonographic findings of craniofacial abnormalities. Level II ultrasonograms manifested alobar HPE and median orofacial cleft. Cytogenetic analysis and fluorescence in situ hybridization (FISH) on cells obtained from amniocentesis revealed partial monosomy 18p and a cryptic duplication of 21q,46,XY,der(18)t(18;21)(p11.2;q22.3), resulting from a maternal t(18;21) reciprocal translocation. The breakpoints were ascertained by molecular genetic analysis. The pregnancy was terminated. Autopsy showed alobar HPE with PMA, pituitary dysplasia, clinodactyly and classical 18p deletion phenotype but without the presence of major typical phenotypic features of Down syndrome. The phenotype of this antenatally diagnosed case is compared with those observed in six previously reported cases with monosomy 18p due to 18;21 translocation. The present study is the first report of concomitant deletion of HPE critical region of chromosome 18p11.3 and cryptic duplication of a small segment of distal chromosome 21q22.3 outside Down syndrome critical region. The present study shows that cytogenetic analyses are important in detecting chromosomal aberrations in pregnancies with prenatally detected craniofacial abnormalities, and adjunctive molecular investigations are useful in elucidating the genetic pathogenesis of dysmorphism. Copyright © 2001 John Wiley & Sons, Ltd.     

Epidemiologic Analysis of Factors Associated with Local Disappearances of Native Ranid Frogs in Arizona

Abstract:  We examined factors that may independently or synergistically contribute to amphibian population declines. We used epidemiologic case–control methodology to sample and analyze a large database developed and maintained by the Arizona Game and Fish Department that describes historical and currently known ranid frog localities in Arizona, U.S.A. Sites with historical documentation of target ranid species ( n = 324) were evaluated to identify locations where frogs had disappeared during the study period (case sites) and locations where frog populations persisted (control sites). Between 1986 and 2003, 117 (36%) of the 324 sites became case sites, of which 105 were used in the analyses. An equal number of control sites were sampled to control for the effects of time. Risk factors, or predictor variables, were defined from environmental data summarized during site surveys and geographic information system data layers. We evaluated risk factors with univariate and multifactorial logistic-regression analyses to derive odds ratios (OR). Odds for local population disappearance were significantly related to 4 factors in the multifactorial model. Disappearance of frog populations increased with increasing elevation (OR = 2.7 for every 500 m, p < 0.01). Sites where disappearances occurred were 4.3 times more likely to have other nearby sites that also experienced disappearances (OR = 4.3, p < 0.01), whereas the odds of disappearance were 6.7 times less (OR = 0.15, p < 0.01) when there was a source population nearby. Sites with disappearances were 2.6 times more likely to have introduced crayfish than were control sites (OR = 2.6, p = 0.04). The identification of factors associated with frog disappearances increases understanding of declines occurring in natural populations and aids in conservation efforts to reestablish and protect native ranids by identifying and prioritizing implicated threats.