Biomarker responses in wild three-spined stickleback (Gasterosteus aculeatus L.) as a useful tool for freshwater biomonitoring: a multiparametric approach

The biochemical response of wild sticklebacks collected in Autumn 2005 at seven stations in the North of France was studied using a set of complementary biomarkers. Here, data on biotransformation of xenobiotics, oxidative stress exposure and damages, neurotoxicity and endocrine disruption are provided. All the sites are characterized by a specific response pattern that allows distinguishing sampling locations. Moreover, these responses are in accordance with data on existing environmental pressures and the chemical analysis of metals performed in surface water. The assessment of individual responses is completed by fish population disturbance monitoring. Based on these measurements, the investigated sites are characterized by different levels of disturbance. This study argues for a multi-parametric approach of aquatic ecosystem contamination based on association between chemical, biochemical and ecological endpoints and provides a testimony of the usefulness of stickleback for this purpose.     

Water quality requirements for sustaining aquifer storage and recovery operations in a low permeability fractured rock aquifer

A changing climate and increasing urbanisation has driven interest in the use of aquifer storage and recovery (ASR) schemes as an environmental management tool to supplement conventional water resources. This study focuses on ASR with stormwater in a low permeability fractured rock aquifer and the selection of water treatment methods to prevent well clogging. In this study two different injection and recovery phases were trialed. In the first phase ~1380 m(3) of potable water was injected and recovered over four cycles. In the second phase ~3300 m(3) of treated stormwater was injected and ~2410 m(3) were subsequently recovered over three cycles. Due to the success of the potable water injection cycles, its water quality was used to set pre-treatment targets for harvested urban stormwater of ≤ 0.6 NTU turbidity, ≤ 1.7 mg/L dissolved organic carbon and ≤ 0.2 mg/L biodegradable dissolved organic carbon. A range of potential ASR pre-treatment options were subsequently evaluated resulting in the adoption of an ultrafiltration/granular activated carbon system to remove suspended solids and nutrients which cause physical and biological clogging. ASR cycle testing with potable water and treated stormwater demonstrated that urban stormwater containing variable turbidity (mean 5.5 NTU) and organic carbon (mean 8.3 mg/L) concentrations before treatment could be injected into a low transmissivity fractured rock aquifer and recovered for irrigation supplies. A small decline in permeability of the formation in the vicinity of the injection well was apparent even with high quality water that met turbidity and DOC but could not consistently achieve the BDOC criteria.     

Medikamentenentsorgung in privaten Haushalten: Ein Faktor bei der Gewässerbelastung mit Arzneimittelwirkstoffen?

Background

Residues of pharmaceuticals are meanwhile detected widely in the aquatic environment. Waste water treatment plants are the main route of entry: after intake the agents or their metabolites are excreted predominantly via urine into the sewage. Largely unknown is, however, if the improper disposal of unused medicines via domestic sanitary devices (toilet or sink) contributes to the measured concentrations in the environment. Aim of this investigation was to clarify to what extent consumers in Germany dispose of unused medicines directly via domestic sewage. In July and August 2006 a representative survey of the population with 2.000 interviewees was performed for this purpose.

Results

The analysis of the survey shows that the disposal of unused medicines via domestic sewage takes place on relevant scales: approx. 16% of the population dispose at least occasionally of unused or expired tablets in the toilet whereas 43% proceed alike with liquid drugs (toilet or sink).

Discussion

A possible reason for this disposal behaviour is the widely inconsistent waste disposal communication by the municipalities and the federal states. The resulting uncertainty about the correct way to dispose of unused medicines gains in importance in the context of the high recycling willingness of the (German) population: appropriate disposal options for drug residues (tablets or liquids) arising from waste separation are often not realised.

Conclusions

Quantitative assessments on the basis of the collected data demonstrate that the established frequency of direct disposal of unused medicines via domestic sewage can partly contribute significantly to the occurrence of pharmaceuticals in the aquatic environment. Crucial parameters are here the excretion rate of the active pharmaceutical ingredient considered and the actual amount of medication waste.

Recommendations

Based on the inquiry results, measures that lead to a more adequate handling of pharmaceutical waste disposal are demanded. The establishment of a consistent and binding disposal standard for unused medicines via return in pharmacies is recommended. This measure needs to be accompanied by a simplification of the established take back systems which partly cause high time and effort for pharmacies.     

Effect of prochloraz fungicide on biotransformation enzymes and oxidative stress parameters in three-spined stickleback (Gasterosteus aculeatus L.)

The aim of this study was to characterize biomarker responses in three-spined sticklebacks exposed to prochloraz (Pcz). For this purpose, adult sticklebacks were exposed for 2 weeks to prochloraz at 0, 10, 50, 100 and 500 microg/L prior to one week of depuration in clean water. At days 7, 14 and 21, several hepatic biomarkers were measured including 7-ethoxyresorufin-O-deethylase (EROD), glutathione-S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), total glutathione (GSH) content and thiobarbituric acid reactive substances (TBARS). Pcz induced a transient increase of antioxidant enzymes and a depletion of glutathione content during the first 7 days of exposure. This study showed that EROD activity and antioxidants were disrupted in a transient manner. GST was rapidly induced in a dose-dependent manner and this induction was persistent and observed also after depuration. GST appeared as a valuable biomarker to assess the exposure to Pcz.     

Residual dynamics of thiacloprid in medical herbs marjoram, thyme, and camomile and in soil

Thiacloprid is a new insecticide of the chloronicotinyl family. To assess its risk after application, residual characteristics of thiacloprid in marjoram, thyme, and camomile and in soil were studied under field conditions. The active ingredient was extracted from the plant material using a mixture of acetone-water. After filtration, the extract was concentrated to the aqueous phase, diluted with water, and portioned against ethyl acetate on a matrix solid phase dispersion column. Thiacloprid was extracted from soil using a mixture of methanol-water, filtered, and re.extracted （clean up） with dichloromethane. The residues were quantified using HPLC-MS-MS. The methods were validated by recovery experiments. Thiacloprid residues in marjoram, thyme, and camomile and in soil persisted beyond 10, 14, 14, and 21 d but no residues were detected after 14, 21, 21, and 28 d, respectively. The data obtained in this study indicated that the biexponential model is more suitable than the first-order function to describe the decline of thiacloprid in fresh marjoram, fresh thyme, and dried camomile flowers with half-life （t1/2） of 1.1, 0.7, and 1.2 d, respectively. However, both the first-order function and biexponential model were found to be applicable for dissipation of thiacloprid in soil with almost the same t1/2 values of 3.5 and 3.6 d. The results indicated that thiacloprid dissipates rapidly and does not accumulate in the tested herbs and in soil.     

Floods and Other Possible Adverse Environmental Effects of Meadowland Area Decline in Former West Germany

6   ha, or 6% of the country's total area. The environmental implications of this large-scale conversion have so far received little attention; the present study examined some of these implications. A review of research on soil physical and chemical aspects of the conversion of permanent grassland into arable land reveals that such a large-scale conversion may have considerable effects upon the environment. For example, due to the mineralization of soil organic matter a release of NO₃ and CO₂ into the environment can be expected on the order of 10 t N and 100 t C per hectare. Environmentally equally severe, if not worse, is the increased amount of surface runoff that can be expected from converted grassland soils in arable land during winter because of surface sealing and soil compaction. This increased surface runoff, in combination with the runoff from other farmland, may be one of the reasons for the growing frequency of floods along major German rivers in recent years. In view of the lasting adverse environmental effects of permanent grassland conversion and the subsidized agricultural surpluses in Germany today, we conclude that a reconversion of arable land into permanent grassland may be beneficial both environmentally and economically.     

Synthesis of [13C]- and [14C]-labeled phenolic humus and lignin monomers

Natural phenolic monomers are ubiquitous in the environment and are involved in the stabilization of atmospheric carbon and the transformation of xenobiotics. Investigations on the stabilization of phenolic carbons and their environmental fate are hampered by the unavailability of commercial [13C]- and [14C]-labeled phenols. Here we report the complete chemical synthesis of the lignin and humus structural monomers p-coumaric, ferulic, and caffeic acids, p-hydroxybenzaldehyde, protocatechualdehyde, vanillin, catechol, and guaiacol, uniformly [13C]- or [14C]-labeled in the aromatic ring, starting from commercially available [U-ring-13C]- or [U-ring-14C]-labeled phenol. The synthesis of these compounds involved selective ortho-hydroxylation of the aromatic ring, Friedel-Crafts alkylation, and Knoevenagel condensation. [U-ring-13C]- or [U-ring-14C]-p-coumaric acid was synthesized via p-hydroxybenzaldehyde with a 75% yield with respect to phenol. Synthesis of [U-ring-13C]- or [U-ring-14C]-ferulic acid, consisting of six single steps via guaiacol and vanillin, had an overall yield of up to 45%. Uniformly ring-labeled caffeic acid was synthesized either via catechol and protocatechualdehyde in five single steps, yielding [U-ring-14C]-caffeic acid with a 37% yield, or via guaiacol, vanillin, and ferulic acid in seven steps, yielding [U-ring-13C]-caffeic acid with an 18% yield. Ferulic acid, [14C]-labeled at beta-C of the propenoic side chain, was synthesized from [2-14C]-malonic acid under Knoevenagel conditions with a 67% yield with respect to malonic acid. Demethylation of the [beta-14C]-ferulic acid with BBr3 in CH3CN resulted in [beta-14C]-caffeic acid with a 62% yield. All [U-ring-13C]-labeled phenolic products were analyzed by 13C nuclear magnetic resonance (13C-NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS).