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Dr. J. F. Bruch P. Metezeau N. Garcia-Fonknechten Y. Richard V. Tricottet B.-L. Hsi A. Kitzis C. Julien E. Papiernik 《黑龙江环境通报》1991,11(10):787-798
Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities. 相似文献
55.
Sustainable development of the earth's limited water and land resources is of paramount importance because of rising world population and existing conflicting demands for these resources. Enormous capital investment has been made in developing these resources, but now there is irrefutable evidence that such developments have led to major resource degradation. This includes problems of salinisation and damages to ecosystems. The countries predominantly affected by human induced salinisation are located in arid and semi-arid regions of the world and include Australia, China, Egypt, India, Pakistan, USSR, and USA. This paper describes the processes of salinisation, its impacts and the use of quantitative methods in salinity investigation and management. Australia is used as a case study of typical salinity problems and as a demonstration of the fruitful application of quantitative methods. The paper concludes that quantitative methods such as surface water and groundwater models are powerful design, management and predictive tools in salinity investigation. However, application of some models, such as those for unsaturated flow and transport and groundwater solute transport, are not widespread due to uncertainties in describing the complexity of the processes and the lack of hydrodispersive data. 相似文献
56.
Evolution of environmental impact assessment as applied to watershed modification projects in Canada
Herman J. Dirschl Nicholas S. Novakowski M. Husain Sadar 《Environmental management》1993,17(4):545-555
This article reviews the application of environmental impact assessment (EIA) procedures and practices to three watershed
modification projects situaled in western Canada. These ventures were justified for accelerating regional economic development,
and cover the period during which public concerns for protecting the environment rapidly made their way into the national
political agenda. An historical account and analysis of the situation, therefore, seems desirable in order to understand the
development of EIA processes, practices, and methodologies since the start of construction of the first project in 1961. This
study concludes that there has been good progress in predicting and evaluating environmental and related social impacts of
watershed modification proposals. However, a number of obstacles need to be overcome before EIA can firmly establish itself
as an effective planning tool. These difficulties include jurisdictional confusions and conflicts, division of authority and
responsibility in designing and implementing appropriate mitigative and monitoring measures, lack of tested EIA methodologies,
and limited availability of qualified human resources. A number of conclusions and suggestions are offered so that future
watershed modification proposals may be planned and implemented in a more environmentally sustainable fashion. These include:
(1) EIA processes must be completed before irrevocable decisions are made. (2) Any major intrusion into a watershed is likely
to impact on some major components of the ecosystem(s). (3) Mitigation costs must form part of the benefit-cost analysis of
any project proposal. (4) Interjurisdictional cooperation is imperative where watersheds cross political boundaries. (5) The
EIA process is a public process, hence public concerns must be dealt with fairly. (6) The role of science in the EIA process
must be at arms length from project proponents and regulators, and allowed to function in the interest of the protection of
the environment and public health and safety.
The views expressed here are the authors’ own and do not necessarily reflect those of FEARO and/or other government agencies
and officials involved in the review of these projects. 相似文献
57.
Assessment of environmental changes in the Orinoco River delta 总被引:1,自引:0,他引:1
H. Echezuría J. Córdova M. González V. González J. Méndez C. Yanes 《Regional Environmental Change》2002,3(1-3):20-35
Major anthropogenic driven changes in the hydrologic and sedimentation patterns of the Orinoco River have had an impact on
environmental conditions in the delta. The abrupt water flow reduction from 3,600 to 200 m3 s–1 in one of its major distributaries resulting from dam construction forced its transformation from a fresh-water body into
a tidal channel with an increase in salinity level (as far as 100 km upstream) and with well-mixed water at the mouth and
estuarine connection to the Paria Gulf. Three different sectors along this distributary can be identified (indicated by the
Na/Cl ratio in the water). As a result, noticeable changes have occurred in the mangrove community which moved about 60 km
further upstream. The changes have also promoted the formation of new islands of sediment progradation at the mouth of this
distributary, where successional colonization and species replacement by different species of grasses and mangroves take place.
Electronic Publication 相似文献
58.
On-going population growth and resulting domestic demand for water require rapid and effective decision-making as regards groundwater management and control of the various sources of salinization and pollution in Coastal aquifers. Sustainability of water resources for utilization by future generations must therefore be a high priority, not only for the purpose of fulfilling needs for water usage but also for bringing people into harmony with their ambient natural environment.The objective of this paper is to propose an empirical approach for prioritization of the needs involved for sustainable aquifer management. The approach involves a schematic format to:(1) develop a global understanding of an aquifer's hydrological and environmental properties in order to delineate appropriate eco-hydrological scenarios and recommend corresponding operational management activities; and(2) emphasize the importance of educating and increasing the awareness of the population involved as to the need for and viability of socially acceptable measures for sustainable management of groundwater and other resources.The psychologist Abraham Maslow utilized a pyramid to illustrate that until people's most basic needs were fulfilled, higher levels of needs would remain irrelevant. This paper postulates a comparable pyramid prioritizing hydrological needs required for progressing towards sustainable groundwater resources. Two sub-regions of Israel's Coastal aquifer in the Sharon region have been presented as representative areas, each characterized by different stress of exploitation. In assessing these sub-regions situation, specific measures have been recommended for achieving and/or maintaining sustainable groundwater resources in light of the ambient environment, and the level of the population on the pyramidal hierarchy of groundwater needs. 相似文献
59.
60.
Critical loads offer a unique way of evaluating impacts of acid deposition by quantifying environmental sensitivity. The critical loads of acidity for UK peat soils have been based upon an arbitrary reduction in pH of 0.2 units. This chemical shift needs to be better related to adverse effects on sensitive biological receptors. It is known that effective precipitation pH equates closely to soil solution pH, and the latter is directly linkable to biotic effects of pH change. On continuation of a long-term experiment assessing impacts of simulated acid rain on peat microcosms in a realistic outdoor environment, Calluna vulgaris continued to flourish at acid deposition loads well above the existing critical load. Calluna plants were harvested and analysed, and acid deposition treatments to the microcosms continued to allow natural vegetation to regenerate. A diverse mixture of moorland plants and bryophytes established at acidity treatments well above the existing critical load, and only a very high acid load resulted in no natural regeneration. A critical effective rain pH value of 3.6 is suggested as a basis for setting critical loads. At this pH, Calluna grows well, and a healthy diverse vegetation community re-establishes when harvested. It is suggested that the peat critical load should be set at the acid load that, at any specific site, would result in a mean effective precipitation pH of 3.6. 相似文献