Glutamine synthetase and protease enzyme activities and growth response of ruminal bacterium Prevotella ruminicola strain B14 to nitrogen source and concentration

Abstract

The objective of this study was to determine the effects of varying nitrogen sources and concentrations upon glutamine synthetase and protease activities in Prevotella ruminicola strain B₁4. Based on growth response it appears that ammonium chloride or pepticase limited P. ruminicola becomes nitrogen limited when nitrogen concentration is at 0.5 mM. However, when casein was provided as the sole source of nitrogen P. ruminicola becomes nitrogen limited at 2.5 mM. Glutamine synthetase activity was measured from mid‐log phase cells grown in either nitrogen‐limited or non‐limited conditions. No activity was detectable in the non‐limited treatments. However, in the N‐ limited treatments, pepticase had the highest activity (20.76 units), followed by ammonium chloride (18.72 units) and casein (14.42 units). Protease activity assays indicated that nitrogen‐limited cultures had higher proteolytic activity than non‐limited cultures. Moreover, these activities appeared to follow the same response pattern as the previously observed glutamine synthetase activities. The results of this study indicate that P. ruminicola strain B, 4 protease activity may be influenced by nitrogen concentration such that activity increases when nitrogen availability decreases.     

Spatial distribution of organochlorine contaminants in soil, sediment, and fish in Bikini and Enewetak Atolls of the Marshall Islands, Pacific Ocean

Several nuclear tests were performed at Enewetak and Bikini Atolls in the Marshall Islands between 1946 and 1958. The events at Bikini Atoll involved several ships that were tested for durability during nuclear explosions, and 24 vessels now rest on the bottom of the Bikini lagoon. Nine soil samples were collected from different areas on the two islands of the atoll, and eighteen sediment, nine fish, and one lobster were collected in the vicinity of the sunken ships. Organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), and polychlorinated terphenyls (PCTs) in these samples were analyzed using gas chromatography/ion trap mass spectrometry (GC/ITMS). The average recoveries ranged from 78% to 104% for the different PCB congeners. The limits of detection (LOD) for PCBs, PCTs, DDE, DDT, and dieldrin ranged 10-50 pg g⁻¹. Some fish from Enewetak contained PCBs at a concentration range of 37-137 ng g⁻¹, dry weight (dw), and most of the soils from Enewetak showed evidence of PCBs (22-392 ng g⁻¹ dw). Most of the Bikini lagoon sediment samples contained PCBs, and the highest was the one collected from around the Saratoga, an aircraft carrier (1555 ng g⁻¹ dw). Some of the fish samples, most of the soil samples, and only one of the sediment samples contained 2,2-bis(4-chlorophenyl)-1,1-dichloroethylene (DDE) and PCBs. In addition to PCBs, the soils from Enewetak Atoll contained PCTs. PCTs were not detected in the sediment samples from Bikini Atoll. The results suggest local pollution sources of PCBs, PCTs, and OCPs.     

Persistence of carbofuran in marine sand and water

Marine sand and seawater samples were collected in March 2002 from Laysan Island in the Hawaiian Islands National Wildlife Refuge, where a small area was contaminated by the carbamate insecticide carbofuran. Carbofuran was still detected at microg g(-1) levels in the Laysan sand after its identification in 1998 and initial observation of the toxicity in 1988. The persistence of carbofuran in the marine sand was investigated in the dark in a 30 degrees C oven, and in distilled deionized water and seawater samples exposed to artificial 300 nm light and to direct sunlight. The laboratory study showed a half-life (t1/2) of approximately 40 days for carbofuran in the native sand and in Ottawa sand. The photolysis of carbofuran was faster in seawater than in distilled deionized water when it was exposed to 300 nm light (t1/2, 0.1 vs. 3.1 h) and to direct sunlight (t1/2, 7.5 vs. 41.6 h). The large difference between the laboratory results and the field observation of carbofuran dissipation suggests that carbofuran degradation at the remote, undisturbed marine site may be governed by its unique environmental factors.     

The sustainable remediation forum

Regulators seem to currently be focusing on green remediation and not sustainable remediation. How can the industry change this perception so that a more holistic approach is followed?     

The Evolution and Role of Non-OPEC Production in the International Oil Market

In 1982, for the first time since OPEC was founded in 1961, its production was surpassed by the non-OPEC countries in the world excluding the centrally planned economies. In that year, for the world as a whole, OPEC provided 35% of the oil consumed. The decline was brought about by a decrease in consumption and by a marked increase in production from non-OPEC sources. It is expected that non-OPEC producers will have the capacity to meet more than half of the world's oil requirements until the early 1990s, at which time OPEC producers will again assume the role of providing over 50% of the world's oil.     

THE STRUCTURE AND PRACTICE OF WATER QUALITY TRADING MARKETS1

ABSTRACT: The use of transferable discharge permits in water pollution, what we will call water quality trading (WQT), is rapidly growing in the U.S. This paper reviews the current status of WQT nationally and discusses the structures of the markets that have been formed. Four main structures are observed in such markets: exchanges, bilateral negotiations, clearinghouses, and sole source offsets. The goals of a WQT program are environmental quality and cost effectiveness. In designing a WQT market, policy makers are constrained by legal restrictions and the physical characteristics of the pollution problem. The choices that must be made include how trading will be authorized, monitored and enforced. How these questions are answered will help determine both the extent to which these goals are achieved, and the market structures that can arise. After discussing the characteristics of different market structures, we evaluate how this framework applies in the case of California's Grassland Drainage Area Tradable Loads Program.     

History and Development of the NRCS Lag Time Equation1

Abstract: Many of the hydrologic methods that are used in engineering practice today resulted from the Spring Flood of 1936, which blanketed the Northeastern portion of the United States. Because of the flood damage that was caused by this rainfall‐snowmelt event, many federal agencies including the U.S. Army Corp of Engineers and the Soil Conservation Service (SCS) implemented the hydrologic theories that were available in the literature at this time and developed hydrologic procedures for design flow estimation. Sherman had recently published his unit hydrograph theory in 1932, and later in 1938 Snyder, who had been charged by the Water Resource Council to develop a synthetic unit hydrograph, published his famous paper. The SCS unit hydrograph theory was developed by Victor Mockus in the late 1950s. Most if not all of the theories at that time reported the rainfall‐runoff process for floods as a surface phenomenon, and as such those theories all required some type of a timing parameter to estimate watershed response time. This article documents the development of the SCS lag equation.     

渤海湾两条河流及其近岸海域水体中DOM的光谱特征及影响因素

应用三维荧光光谱结合吸收光谱分析,研究了2019年夏季天津市大清河-独流减河水系和河北省黄骅市捷地减河及其河口区水体中溶解有机物（DOM）的空间分布特征、来源及其影响因素。紫外-可见光谱结果表明,研究区水体的SUVA₂₅₄与盐度有显著正相关关系;两条河流的下游水体中S_275-295均值均大于上游和中游。平行因子分析结果表明,两条河流均检测到两种类腐殖质组分C1（240,300/390 nm）和C3（260,335/477 nm）以及一种类蛋白质组分C2（225,275/339 nm）。在大清河-独流减河水系及其近岸海域水体中,这三种组分与盐度呈显著负相关关系,其中由于人为输入（TN和TP含量高,工业排污）影响导致C2与盐度的相关性弱于C1和C3。该水系水体在低盐度时C2的占比高于捷地减河,主要受到工农业废水输入与其支流子牙河汇入的共同影响,而捷地减河主要受农业废污水输入的影响。此外,自然因素（盐度、海水混合）和人为因素（人为输入、筑坝拦截）的不同导致渤海湾两条入海河流中有色溶解有机质（CDOM）的光学特性和混合行为出现显著差异。其中筑坝拦截导致独流减河坝前水体的CDOM平均分子量低于下游水体,并且增强了河口区陆源类腐殖质的去除作用。综上,三维荧光光谱结合平行因子分析能判别河流局部条件的差异对整体CDOM空间分布特征的影响,研究结果可为渤海湾地区河流及河口水体CDOM生物地球化学过程研究提供基础数据。     

Market‐based conservation: Aligning static theory with dynamic systems

This paper provides a broad evaluation of the implications of market‐based conservation (MBC) strategies from economic, social and ecological perspectives. After reviewing the economic theory that underlies MBC initiatives, we develop a list of approaches that have been labeled as “market‐based”, and categorize them according to the degree to which they are free‐standing markets or require the intervention of the state. A multidisciplinary critique reveals the potential problems and pitfalls of MBC are due to a lack of ability to deal with dynamic systems in the real world. The dynamics of these three systems can undermine the intended goals of MBC.     

Vegetation and climate

Over the last two centuries, man's activities have caused a 30% increase in the atmospheric concentration of CO₂, with continued increases seeming inevitable. This change in CO₂ concentration will act on vegetation, both directly and indirectly through global climatic change. It is well established that, on a global scale, patterns of vegetation and climate are closely correlated. Such correlations indicate that climatic change will cause the distribution of vegetation to change. However, the use of correlations for predicting vegetation responses to climatic change is fundamentally unreliable because correlations have no mechanistic underpinning of causation. This paper outlines a mechanistic model for predicting the equilibrium state between current climate and vegetation. It is also used to indicate the sensitivity of global vegetation to the changed climate associated with a doubled CO₂—greenhouse scenario. The interpretation of this static model is discussed in terms of rates and patterns of vegetation change.