“环境监测实习”实践教学的改革和实践

在建设校内外“环境监测实习”实践基地的基础上,形成将区域环境质量监测、城市环境监测站和污水处理厂参观等相结合的新型“环境监测实习”实践教学内容体系,建立动手实践、参观和讲座等联合的新的教学方式,并建立激发学生全面发展的教学评分体系,强化学生实践能力、综合素质和创新能力的培养,从而将实践、教学和科研紧密结合,为学生顺利走入相应工作岗位奠定基础。     

Research progress on occurrence forms and microbial transformation of mercury北大核心CSCD

汞是一种高毒性且具有持久性的重金属污染物,汞污染的治理与修复在近几十年一直是国内外研究热点.了解微生物对汞赋存形态的转化作用,对汞污染的治理与修复具有重要意义.总结汞的不同赋存形态、毒性及对应的常用分析方法,其中甲基汞(methyl mercury,MeHg)是毒性最强的汞形态之一.环境中汞的化学形态能发生转化,尤其以微生物驱动的汞的甲基化、MeHg的去甲基化和汞的氧化还原最为常见.依据汞转化类型将汞转化相关微生物分为汞甲基化、MeHg去甲基化、汞还原、汞氧化等类群,将对应的汞转化作用机制分为基于hgcAB基因的汞甲基化、基于mer操纵子基因的MeHg去甲基化和Hg2+还原、胞内过氧化氢酶介导的Hg0氧化.微生物汞转化过程不仅受到pH和温度的显著影响,而且还受到汞的赋存形态和游离汞的浓度、微生物种/群结构与功能、矿物种类、中间体和次生产物及其交互作用的影响,基于此,提出正确客观表征汞的微生物转化过程需要综合分析微生物组和矿物组的变化规律及其交互作用的综合效应.针对酸性矿山废水(AMD)极端环境微生物汞转化研究的不足,未来的工作将聚焦结合多组学手段、同步辐射谱学和密度泛函理论(DFT)计算等分析技术研究汞赋存形态的微生物转化过程,分析和阐明汞转化中间体的键合作用方式和转化机制,从而为AMD汞污染的预防、治理和修复提供依据.(图2表2参107)     

Photosynthetic characteristics and reproductive strategies of Polygonum viviparum at different altitudes in the Qilian Mountains北大核心CSCD

Polygonum viviparum, which reproduces sexually and asexually, is widely distributed in the Qinghai-Tibet Plateau, and its reproduction strategies are very sensitive to changes in the environment. This study aimed to elucidate the effect of altitude on the photosynthetic characteristics and reproductive strategies of P. viviparum. This study was conducted to investigate the responses of photosynthetic, vegetative, and reproductive traits of P. viviparum populations along six altitudinal gradients in the eastern part of the Qilian Mountains in China. Our results indicated that, with increasing altitude, the net photosynthetic rate of P. viviparum showed an increasing trend, reaching its maximum value (14.39 μmol m–2 s–1) at 3 700 m above sea level. The transpiration rate showed an increasing trend, followed by a decreasing trend, and the intercellular CO2 concentration did not differ significantly between altitudes. With increasing altitude, the plant height and leaf area of P. viviparum showed a downward trend, the aboveground and underground biomass decreased, and the specific leaf area initially decreased and then increased. However, the leaf greenness index showed an upward trend, and the number of stomata in the upper and lower epidermis of leaves initially increased and then decreased. With increasing altitude, the proportion of inflorescence length per plant (the ratio of inflorescence length to plant height), the proportion of bulbil length per inflorescence (the ratio of bulbil length to inflorescence height), and the proportion of the number of bulbils per inflorescence (the ratio of the number of bulbils to the total number of flowers and bulbils) showed an increasing trend. Air temperature and light intensity are the major environmental factors affecting the photosynthetic characteristics and functional traits of P. viviparum. Thus, P. viviparum is exposed to more environmental stresses and obtains less energy when altitude increases, but it adapts to the harsh alpine environment by increasing the photosynthetic capability per unit area. With increasing altitude, P. viviparum populations may be sustained by investing less energy in vegetation and more in reproduction, especially asexual reproduction. © 2022 Science Press. All rights reserved.     

Development of lightweight aggregate from dry sewage sludge and coal ash

In this study, dry sewage sludge (DSS) as the principal material was blended with coal ash (CA) to produce lightweight aggregate. The effects of different raw material compositions and sintering temperatures on the aggregate properties were then evaluated. In addition, an environmental assessment of the lightweight aggregate generated was conducted by analyzing the fixed rate of heavy metals in the aggregate, as well as their leaching behavior. The results indicated that using DSS enhanced the pyrolysis–volatilization reaction due to its high organic matter content, and decreased the bulk density and sintering temperature. However, the sintered products of un-amended DSS were porous and loose due to the formation of large pores during sintering. Adding CA improved the sintering temperature while effectively decreasing the pore size and increasing the compressive strength of the product. Furthermore, the sintering temperature and the proportion of CA were found to be the primary factors affecting the properties of the sintered products, and the addition of 18–25% of CA coupled with sintering at 1100 °C for 30 min produced the highest quality lightweight aggregates. In addition, heavy metals were fixed inside products generated under these conditions and the As, Pb, Cd, Cr, Ni, Cu, and Zn concentrations of the leachate were found to be within the limits of China’s regulatory requirements.     

钢棒去皮机除尘器设计

钢棒去皮机是通过砂轮磨削除去钢棒表面铁锈的一种设备，在去皮过程产生的大量金属粉尘，导致环境污染，为此设计喷淋塔式除尘器作为钢棒去皮机的除尘装置。通过选择和计算的运行参数，设计了喷淋塔的结构和尺寸。对设计的除尘器效率验算结果表明：该除尘器可以满足去皮机环保要求，除尘效率达99．7％。     

合芜蚌试验区排污权交易制度建立之构想

排污权交易的实践证明了排污权交易可以有效地保护环境,同时也提出了亟需对其适用条件和建设模式进行探索的现实命题。文章以合芜蚌自主创新试验区为例,在分析了合芜蚌试验区内推行排污权交易制度的可行性基础上,提出了区域性排污权交易的构建模式。     

基于RBI技术的海洋平台设备完整性分析

通过对容器和管道的基础数据和工艺物流数据的整理分析,应用基于风险的检验(RBI)风险分析技术和专业风险评估软件,对海上采修一体化平台上9台容器设备和27条管道开展损伤机理和腐蚀机理等主要指标的分析,计算得出容器和管道的失效可能性、失效后果、风险等级、危害程度等量化结果,根据RBI评估结果制定了设备的检验策略,提出了开展设备设施完整性管理的建议.     

食蚊鱼(Gambusia afinis)cat、gapdh和gst基因的克隆及其在生态毒理学中的应用

根据Ensembl、Genbank登录的鱼类cat、gapdh和gst基因的CDS序列设计普通PCR扩增引物,寻找食蚊鱼的cat、gapdh和gst基因的c DNA片段,并根据定量引物设计要求设计出相应的SYBR Green I荧光定量RT-q PCR引物,建立了食蚊鱼cat、gapdh和gst基因的SYBR Green I荧光定量RT-q PCR方法。该方法在104~108数量级范围内有良好线性关系(R=0.999~1.000);熔解曲线显示扩增产物特异性良好,均为单一峰值;质粒标准品最高浓度与最低浓度的批内试验变异系数与批间试验变异系数均低于2%。利用该方法监测和评价环境污染物对水生生物的影响,选择了水体中常见典型药物污染物——双氯芬酸,研究其对食蚊鱼抗氧化基因表达的影响。结果表明,雌性食蚊鱼暴露在不同浓度双氯芬酸钠(0.005、0.05、0.5和5 mg·L-1)24 h后,其肝脏cat、gapdh和gst的mRNA呈现显著变化,相对于对照组,在低浓度0.005 mg·L-1时,cat与gst mRNA的表达量均有极显著上升(p0.01),而其它浓度均极显著下降(p0.01)。试验表明该方法具有快速、精确、灵敏度高的优点,可为利用该类小型鱼类的原位污染物的生物监测和生态毒理评价提供有力的技术支持。     

Decolouring mechanism of Zhejiang diatomite. Application to printing and dyeing wastewater

We report the adsorption isotherm of methylene blue on the surface of Zhejiang and Jilin diatomite. The relationship between the adsorption isotherm and diatomite pore structure is discussed. The degree of decolouring was found to be strongly related to pH and isoelectrical value. Furnace ash residue and diatomite were found to be very effectively treating printing and dyeing wastewater.     

Size fractionation of organic matter and heavy metals in raw and treated leachate

This study characterized the organic matter and heavy metals in the leachate from two typical municipal solid waste (MSW) sanitary landfills in China, the recently established (3-year-old) Liulitun landfill and the mature (11-year-old) Beishenshu landfill, using a size fractionation procedure. The organic matter of all raw and treated leachate samples primarily existed in a truly-dissolved fraction with an apparent molecular weight (AMW) of <1 kDa, and its percentage decreased with an increase in overall AMW. The leachate from the newer landfill had a higher percentage of truly-dissolved organic matter. After anaerobic treatment, this leachate had a similar size fraction of organic matter to that seen for the raw leachate of the mature landfill. Biochemical processes had different removal efficiencies for various types of AMW organic matter, and the concentration of moderate AMW organic matter appeared to increase throughout these processes. Most of the heavy metals existed in a colloidal fraction (AMW >1 kDa and particle size <0.45 μm). The behaviors of different species of heavy metals had large variations. The size fractions of heavy metal species were significantly affected by treatment processes and landfill age, except for Zn. The concentration ratio of heavy metals to organic matter was maximal in the colloidal fraction and showed an inverse change to that seen for organic matter concentration changes caused by biochemical processes. Consequently, the pollution levels of heavy metals were substantially increased by treatment processes, although their concentrations decreased.