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341.
Maria Rönnqvist Thedi Ziegler Carl-Henrik von Bonsdorff Leena Maunula 《Food and environmental virology》2012,4(1):26-33
Recent events have shown that humans may become infected with some pathogenic avian influenza A viruses (AIV). Since soil
and water, including lakes, rivers, and seashores, may be contaminated by AIV excreted by birds, effective methods are needed
for monitoring water for emerging viruses. Combining water filtration with molecular methods such as PCR is a fast and effective
way for detecting viruses. The objective of this study was to apply a convenient method for the detection of AIV in natural
water samples. Distilled water and lake, river, and seawater were artificially contaminated with AIV (H5N3) and passed through
a filter system. AIV was detected from filter membrane by real-time RT-PCR. The performance of Zetapor, SMWP, and Sartobind
D5F membranes in recovering influenza viruses was first evaluated using contaminated distilled water. SWMP, which gave the
highest virus recoveries, was then compared with a pre-filter combined GF/F filter membrane in a trial using natural water
samples. In this study, the cellulose membrane SMWP was found to be practical for recovery of AIVs in water. Viral yields
varied between 62.1 and 65.9% in distilled water and between 1 and 16.7% in natural water samples. The borosilicate glass
membrane GF/F combined with pre-filter was also feasible in filtering natural water samples with viral yields from 1.98 to
7.33%. The methods described can be used for monitoring fresh and seawater samples for the presence of AIV and to determine
the source of AIV transmission in an outbreak situation. 相似文献
342.
Sara Romani Seyed Reza Mohebbi Seyed Masoud Hosseini Pedram Azimzadeh Mohsen Vahedi Faramarz Derakhshan Mohammad Reza Zali 《Food and environmental virology》2012,4(1):1-5
Noroviruses are one of important agents that cause acute viral gastroenteritis worldwide. These viruses are belonging to Caliciviridae
family and are genetically diverse. To date, there is no valuable data about prevalence of norovirus infection and the dominant
genogroup/genotype among Iranian population. The objective of this study was to determine the frequency of norovirus infection
in Iranian patients with gastroenteritis referred to three hospitals of Tehran and to specify the dominant genogroup/genotype
of this virus among our study population. A total of 293 patients with acute gastroenteritis were included in the study. Detection
of norovirus was performed using RT-PCR method and confirmed by direct sequencing with specific designed primers for capsid
region of norovirus genome. Phylogenetic analysis was performed using the neighbor-joining method. Norovirus strains identified
in our study were subsequently categorized according to previously defined genogroup/genotypes. Of these, norovirus GII was
dominant genogroup. Sixty-five percent (17 of 26) of positive samples were determined as GII and 35% (9 of 26) were determined
as GI, respectively, in 2008–2009. And among 8 sequenced strains of genogroup II the most frequent genotype was GII.3. The
results of this study indicated that norovirus must be considered as one of the infectious causes of acute gastroenteritis
among Iranian population. We also found that GII.3 is more prevalent in our study population. To the best of our knowledge
there is limited data about the role of noroviruses in children and adults’ acute gastroenteritis among Iranian patients and
this prevalence and genotyping report of norovirus infection could be remarkable for further studies. 相似文献
344.
本文主要介绍了我国的活火山、火山灾害情况及国内外的火山预警政策。在此基础上讨论了我国火山地区预警政策的组成结构,认为完整的火山地区预警政策应该包括活火山识别、火山风险评价与灾害区划图、制定火山应急预案、火山防灾减灾知识宣传、火山监测和灾害预报、火山灾害预警等级划分、火山减灾工程措施以及预警响应措施等方面,并对火山预警政... 相似文献
345.
Mingsong Wu Junli Huang Yuling Zhang Shijie You Shaofeng Li Zhilin Ran Yu Tian 《Frontiers of Environmental Science & Engineering in China》2012,6(1):75-81
Microcystins, which represents one kind of cancerogenic organic compounds, is abundant in eutrophication water. The effects
of reaction factors on chlorine dioxide (ClO2) for removal of low-concentration Microcystin-LR, Microcystin-RR, and Microcystin-YR in water as well as the reaction mechanisms
was investigated by using enzyme-linked immunosorbent assay (ELISA) kit and gas chromatography-mass spectrometry (GC-MS).
The results showed that MC-LR, MC-RR, and MC-YR could be efficiently decomposed by ClO2. The degradation efficiency was shown positively correlated to the concentration of ClO2 and reaction time; while the effect of reaction temperature and pH is slight. The kinetic constants and activation energies
of the reaction of MC-LR, MC-RR, and MC-YR with ClO2 are determined as 459.89, 583.15, 488.43 L·(mol·min)−1 and 64.78, 53.01, 59.15 kJ·mol−1, respectively. As indicated by high performance liquid chromatography mass spectrometer (HPLC-MS) analysis, degradation should
be accomplished via destruction of Adda group by oxidation, with the formation of dihydroxy substituendums as end products.
This study has provided a fundamental demonstration of ClO2 serving as oxidizing disinfectant to eliminate microcystins from raw water source. 相似文献
346.
文章采用析因设计的方法,研究沉积物水浸提液掺杂的多种重金属Cu、Cd、Zn、Pb和Ni对发光菌的联合毒性。通过对试验结果进行方差分析和多元线性回归,建立了重金属浓度和发光菌发光抑制率的关系模型:T=97.983Cu+41.111Cd-27.297Zn+129.869Pb+16.715Ni-5 803.639Cu*Cd+2 270.182Cd*Zn-6 411.870Cd*Pb-675.052Cd*Ni-3 108.544Pb*Ni+15 9552.434Cd*Pb*Ni+274 718.434Zn*Pb*Ni。模型分析结果表明:Zn对联合毒性产生负的影响,Cu、Cd、Pb、Ni对联合毒性具有正的贡献;Cd*Zn二元交互作用和Cd*Pb*Ni、Zn*Pb*Ni三元交互作用表现为协同作用,Cu*Cd、Cd*Pb、Cd*Ni、Pb*Ni二元交互作用表现为拮抗作用,而重金属四元和五元交互作用对联合毒性的贡献不显著。上述重金属联合毒性的作用可为沉积物中重金属复合污染控制提供参考。 相似文献
347.
348.
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350.
A framework for analyzing climate change adaptations as actions 总被引:1,自引:0,他引:1
Klaus Eisenack Rebecca Stecker 《Mitigation and Adaptation Strategies for Global Change》2012,17(3):243-260
Developing generalized theories about adaptation to climate change requires common concepts to map different adaptation situations.
The paper aims to contribute to this endeavor by presenting a novel framework that conceptualizes adaptations to climate change
as actions. The framework is intended to systematically analyze the actor relations involved in adaptations and the barriers
to their implementation. By combining established scientific action theories with terminology from the Intergovernmental Panel
on Climate Change (IPCC) in an innovative way, it can be used to clarify the notion of adaptation used in adaptation assessments.
The framework’s potential is illustrated by a case study on cooling water management in the river Rhine catchment and by the
elucidation of some prominent concepts in adaptation research. We show that by framing adaptations as actions, the purpose
of adaptations and how they tend to connect up in means-ends-chains becomes crucial. Actors can take different functional
roles as exposure unit, operator and receptor of adaptation. A mismatch of these roles can lead to barriers to adaptation,
of which we deduce four types: complex actor relations, missing operators, missing means and unemployed means. The case study
yields a complex bundle of adaptations, and shows that the potential barriers involved are quite diverse. There is thus no
blueprint solution. Although we identify entry points for adaptation, the analysis leads to a skeptical conclusion for adapting
cooling water management in the whole Rhine catchment. 相似文献