Prenatal cytogenetic diagnosis after transabdominal chorionic villus sampling in the first trimester

First trimester prenatal cytogenetic diagnosis was attempted in 350 pregnancies after trans-abdominal chorionic villus sampling. The cytogenetic investigation was performed using both a short-term method (24 h incubation) and cell culture. Adequate samples were obtained in 99·1 per cent and in all these cases the fetal karyotype was established. A chromosome abnormality was found in 2·0 per cent of cases. A discrepancy between the karyotype obtained after 24 h incubation and the karyotype in cell culture was observed in 2·3 per cent. Maternal cell contamination in the cultures was confirmed in 13 of 181 cases where the 24 h incubation revealed a male karyotype. Studies of culture morphology showed that colonies of convoluted cells may serve as a marker for contamination with maternal cells in culture. For the present, we recommend using a short-term method as well as cell culture for cytogenetic investigation until the problems with karyotype discrepancy and maternal cell contamination have been further clarified.     

A routine method for storing lymphocytes for repeated isolation of DNA

A protocol for easy storage and later expansion of lymphocyte populations is given. Compared with methods using transformed cell lines, the method has a number of advantages for repeated production of cells for the isolation of DNA in amounts sufficient for use in diagnostic DNA technology.     

2000年以来黄河流域土地市场化时空格局演变及驱动因素

基于土地市场交易结构的视角,测算2000-2016年黄河流域104个地级以上城市一级土地市场化程度,综合利用Global Moran's I、G^*_i指数空间统计模型对其时空格局演变特征进行分析,运用灰色关联度分析模型探究土地市场化时空格局演变的主要驱动因素。研究发现：2000-2016年,黄河流域土地市场化水平表现出先持续上升后不断下降的阶段性变化特征,呈现了“东高西低”的空间分异格局。黄河流域土地市场化水平整体具有显著的空间自相关性,土地市场化热点区域以下游主要城市为核心,经历了先加速蔓延、后缓步收缩的时空演进历程。黄河流域土地市场化水平的时空格局演变主要由经济发展水平、产业结构升级、固定资产投资、政府财政收支比、外商直接投资以及区位条件等因素共同驱动,但受我国社会经济发展的阶段性影响,各因素在不同年份的影响程度存在显著的差异性。     

Is the Newly Described Vietnamese Bovid Pseudonovibos spiralis a Chamois (Genus Rupicapra)?

 The “Linh Duong” (Pseudonovibos spiralis), a newly described Vietnamese bovid, has been shown to be related to the Caprinae on the basis of the mitochondrial cytochrome b gene sequencing from horn fragments. To integrate the greatest available biodiversity we performed a new phylogenetic analysis including all genera of Caprinae. Unexpectedly, Pseudonovibos is found to be robustly associated with Rupicapra, and closer to the Alpine than to the Pyrenean chamois. Several clues led us to interpret this result as a carry-over DNA contamination of Pseudonovibos by Rupicapra. Received: 19 July 1999 / Accepted in revised form: 20 October 1999     

不同声学元件在公路声屏障中的应用

通过对公路声屏障不同声学元件声学性能的比较研究，阐明了两种声学元件在治理交通噪声中的差别，指出吸声共振型声学元件将是未来公路声屏障的发展方向，该研究结论对于开展公路声障声学设计有较为重要的参考价值     

Upgrading of organic waste: production of the copolymer poly-3-hydroxybutyrate-co-valerate by Ralstonia eutrophus with organic waste as sole carbon source

Two types of fermented organic waste (trade and industry waste and fruit and vegetable waste) were successfully used as a sole carbon source to produce poly-3-hydroxybutyrate-co-valerate (PHBV) by Ralstonia eutrophus (formerly Alcaligenes eutrophus) via oxygen limitation.The production of PHBV could be optimized by optimizing the oxygen transfer through the fermentor. Thereby, a peak concentration of 1.1 g PHBV per liter cell suspension, 40 w% of cell dry weight, was obtained at an aeration rate of 0.24 mol O₂/h·kg biomass. The yield of PHBV on the fatty acid concentration in the organic waste was 0.16 g polymer/g volatile organic matter. The process obtained, compares well with the commercial production process of PHBV based on glucose.     

Carbon Dioxide Emission Reduction Scenarios in Mexico for Year 2005: Industrial Cogeneration and Efficient Lighting

An analysis of the impacts on Mexican energy demand and associated carbon dioxide (CO₂) emissions in the year 2005 due to efficient lighting in the commercial and residential sectors and cogeneration in the industrial sector is presented. Estimation of CO₂ abatement costs and an incremental cost curve for CO₂ mitigation options are considered. These technologies are cost effective opportunities, and together are projected to reduce CO₂ emissions in 2005 by nearly 13 percent. Implementation of efficient lighting is already part of the demand side management (DSM) programs of the Mexican state-owned utility. However, there are important barriers that may hinder the implementation of large scale cogeneration plants.     

酸沉降破坏材料造成的经济损失的估算研究

酸沉降对材料的破坏和其经济损失估算是国际上酸沉降研究领域听一个重要课题，国内在经方面的研究较少，文章通过对“两广酸沉降破坏材料造成的经济损失的估算”研究，提出了了一套较实用的经济损失估算方法。