利用MALDI-TOF MS技术简便、快速检测水华蓝藻的方法研究

以水华蓝藻为研究对象,用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术对其进行测定分析并获得了蛋白质指纹图谱。对铜绿微囊藻标准株(NIES-843)样品通过3种前处理方法得到的质谱图进行对比,确定了质谱分析的样品前处理方法,建立了利用MALDI-TOF MS技术简便、快速检测水华蓝藻的方法。对在水华中出现的4种不同蓝藻进行MALDI-TOF MS分析,结果表明各种蓝藻具有其特征性波谱,可据此对水华蓝藻进行区分和鉴定。该方法快速、简便、精确、可程序化,具有广泛的应用前景。     

高原机场飞机噪声监测与计算结果比较分析

高原机场飞机架次较少,机型单一,飞行周期稳定,以及高原特殊的环境特征,有时会导致现场实际噪声监测数据无效。应用综合噪声模型(INM)软件对青海玉树机场飞机噪声进行计算,其结果与监测结果比较显示,计算值和监测值误差在±6 d B的范围以内。探讨提出采用飞机噪声计算模式,可以为高原机场飞机噪声管理提供可靠技术支撑。     

一种淡水大型底栖无脊椎动物采样器研究

研制出一种长方体的十字筐采样器,在采样器内分别填装石、砂、底泥和水草4种基质,利用大型底栖无脊椎动物对生境的选择性进行样本被动采集。基质越多,采样器采集的生物种类越多,生物量也更丰富。用新研制的十字筐采样器和传统的篮式采样器对松花江流域7个采样点进行大型底栖无脊椎动物对比采样,结果显示:十字筐采样器是一种优良的采样器,适合在松花江流域乃至全国推广应用。     

典型湖泊水华特征及相关影响因素分析

通过2011-2015年对太湖、巢湖和滇池水华高发季节的连续监测,以藻类密度和水华面积为判据评价了3个湖体的水华情况及变化趋势,探讨了水华发生的主要影响因素。结果表明：太湖水华程度以"轻度水华"为主,巢湖水华程度以"轻微水华"为主,滇池水华程度以"中度水华"为主;太湖、巢湖和滇池水华规模均以"零星性水华"为主;太湖和巢湖藻类密度与水温、pH、溶解氧、总氮、总磷和高锰酸盐指数均呈显著正相关,与透明度呈显著负相关,与氨氮无显著相关性;滇池藻类密度与水温、总磷和高锰酸盐指数均呈显著正相关,与透明度和氨氮呈显著负相关,与pH、溶解氧和总氮无显著相关性。     

建设项目竣工环境保护验收现状及建议

简述了当前建设项目竣工环境保护验收的现状,指出竣工环境保护验收存在权责不明、事中监管缺失、事后监管落地难、监测数据"失真"等问题,提出应落实建设单位的主体责任,规范建设项目竣工环境保护企业自行验收,加强事中、事后监管,整合建设单位监测数据,使验收监测数据发挥实效。     

Nitrogen enrichment in runoff sediments as affected by soil texture in Beijing mountain area

Enrichment ratio (ER) is widely used in nonpoint source pollution models to estimate the nutrient loss associated with soil erosion. The objective of this study was to determine the ER of total nitrogen (ER_N) in the sediments eroded from the typical soils with varying soil textures in Beijing mountain area. Each of the four soils was packed into a 40 by 30 by 15 cm soil pan and received 40-min simulated rainfalls at the intensity of 90 mm h^?1 on five slopes. ER_N for most sediments were above unity, indicating the common occurrence of nitrogen enrichment accompanied with soil erosion in Beijing mountain area. Soil texture was not the only factor that influenced N enrichment in this experiment since the ER_N for the two fine-textured soils were not always lower. Soil properties such as soil structure might exert a more important influence in some circumstances. The selective erosion of clay particles was the main reason for N enrichment, as implied by the significant positive correlation between the ER of total nitrogen and clay fraction in eroded sediments. Significant regression equations between ER_N and sediment yield were obtained for two pairs of soils, which were artificially categorized by soil texture. The one for fine-textured soils had greater intercept and more negative slope. Thus, the initially higher ER_N would be lower than that for the other two soils with coarser texture once the sediment yield exceeded 629 kg ha^?1.     

The dynamics of toxic and nontoxic Microcystis during bloom in the large shallow lake,Lake Taihu,China

Lake Taihu is a large shallow freshwater lake (surface area 2,338 km², mean depth 1.9 m) in China, which has experienced toxic cyanobacterial bloom dominated by Microcystis annually during the last few decades. In the present study, the dynamics of toxic and nontoxic Microcystis in three sampling stations (Meiliang Bay (site N2), Gonghu Bay (site N4), and the lake center area (site S4)) were quantified using quantitative real-time PCR (qPCR) during bloom periods from April to September, 2010. Our data showed that the abundance of toxic Microcystis and the toxic proportion gradually increased from April to August in water samples and reached the peak in August. During the study period, toxic Microcystis genotypes comprised between 26.2 and 64.3, between 4.4 and 22.1, and between 10.4 and 20.6 % of the total Microcystis populations in the three sampling sites, respectively. Correlation analysis suggested that there was a strong positive relationship between total Microcystis, toxic Microcystis and the toxic proportion. Chlorophyll a, total phosphorus, and water temperature were positively correlated with the abundances of total Microcystis and toxic Microcystis. Furthermore, the toxic proportion was positively correlated with total phosphorus (P?<?0.05) and water temperature (P?<?0.01), showing that global warming together with eutrophication could promote more frequent toxic blooms.