Isolation of single circulating trophoblasts from maternal circulation for noninvasive fetal copy number variant profiling

Objective

To develop a multi-step workflow for the isolation of circulating extravillous trophoblasts (cEVTs) by describing the key steps enabling a semi-automated process, including a proprietary algorithm for fetal cell origin genetic confirmation and copy number variant (CNV) detection.

Methods

Determination of the limit of detection (LoD) for submicroscopic CNV was performed by serial experiments with genomic DNA and single cells from Coriell cell line biobank with known imbalances of different sizes. A pregnancy population of 372 women was prospectively enrolled and blindly analyzed to evaluate the current workflow.

Results

An LoD of 800 Kb was demonstrated with Coriell cell lines. This level of resolution was confirmed in the clinical cohort with the identification of a pathogenic CNV of 800 Kb, also detected by chromosomal microarray. The mean number of recovered cEVTs was 3.5 cells per sample with a significant reverse linear trend between gestational age and cEVT recovery rate and number of recovered cEVTs. In twin pregnanices, evaluation of zygosity, fetal sex and copy number profiling was performed in each individual cell.

Conclusion

Our semi-automated methodology for the isolation and single-cell analysis of cEVTS supports the feasibility of a cell-based noninvasive prenatal test for fetal genomic profiling.     

Evaluation of bio-aerosols type,density, and modeling of dispersion in inside and outside of different wards of educational hospital

Environmental Science and Pollution Research - Exposure to bioaerosols in the air of hospitals is associated with a wide range of adverse health effects due to the presence of airborne...     

清河流域水生态维持功能评估

利用野外样点调查数据、遥感解译数据和多年降水监测数据,从生物多样性维持功能、生境维持功能和水资源支持功能3个方面对2011年清河流域水生态维持功能进行评估,为"十二五"期间水专项在清河流域开展水环境管理技术实施示范效果评估奠定基础。结果表明:2011年清河流域生物多样性维持功能和生境维持功能较好,而水资源支持功能较差。建议通过开展生态保护工程增加植被覆盖度,增强水资源支持功能和水土保持能力。     

Use of Small-area Data to Support a Community Agenda in Boston Chinatown

Boston Chinatown is a community that grapples with a constant pressure from the downtown area to build new and larger buildings. The effort to preserve the residential aspect of Chinatown has used data analysis in recent years by forming partnerships with academics. This has allowed for more sophisticated studies of traffic injuries, scientific surveys of residents about environmental health, the measurement of noise levels and the construction of physical and electronic mapping of data. While not the only factor contributing to the community's efforts to question the role of development, these data have added useful tools that reduce, but do not eliminate, the disparity between what the community brings to discussions about the environmental impact of development and what the developers have at their disposal.     

Alteration of sediment organic matter in sediment microbial fuel cells

The alteration of physico-chemical properties of sediment organic matter (SOM) incubated under current-harvesting conditions as well as no-current producing conditions over 120 days using sediment microbial fuel cell systems was examined. The SOM was microbially oxidized under anaerobic conditions with an electrode serving as a terminal electron acceptor. It was found that SOM around the electrochemically-active electrodes became more humified, aromatic, and polydispersed, and had a higher average molecular weight, along with its partial degradation and electricity generation compared to that for the original sediment. These changes in SOM properties were analogous to those commonly observed in the early stages of the SOM diagenetic process (i.e. humification). Such a humification-like process was evidently more stimulated when electrical current was produced than no-current condition. These new findings associated with microbially-catalyzed electricity generation may present a potential for the energy-efficient remediation, monitoring, and/or management of the geo-environment.     

Arsenate (As) uptake by and distribution in two cultivars of winter wheat (Triticum aestivum L.)

Two cultivars of winter wheat (Triticum aestivum L.) (Jing 411 and Lovrin 10) were used to investigate arsenate (As) uptake and distribution in plants grown in hydroponic culture and in the soil. Results showed that without As addition, Lovrin 10 had higher biomass than Jing 411 in the soil pot experiment; in the hydroponic experiment Lovrin 10 had similar root biomass to and lower shoot biomass than Jing 411. Increasing P supply from 32 to 161 microM resulted in lower tissue As concentrations, and increasing As supply from 0 to 2,000 microM resulted in lower tissue P concentrations. Increasing P supply tended to increase shoot-to-root ratios of As concentrations, and increasing As supply tended to decrease shoot-to-root ratios of As concentrations. Both cultivars invested more in root production under P deficient conditions than under P sufficient conditions. Lovrin 10 invested more biomass production to roots than Jing 411, which might be partly responsible for higher shoot P and As concentrations and higher shoot-to-root ratios of As concentrations. Moreover, Lovrin 10 allocated less As to roots than Jing 411 and the difference disappeared with decreasing P supply.     

Using respirometer in biodegradation phenomenon of natural organic matters

This study conducted in monitoring respirometer oxygen consumption of aerobic microorganism during biodegradation processes of ozonated organic matters, which can estimate both biodegraded efficiency and coefficient of natural organic matters (NOMs) in water source. It can be proposed that different ozone dosage might change biodegradation characteristics of organic matters. The result reveals that higher ozone dosage may cause higher biomass yield coefficient of microorganism, and cultured microorganism may easily utilize biodegradation organic matters (BOMs) produced by ozonation, finally increasing overall removal efficiency. Therefore, using respirometer to evaluate the production of BOMs by ozonation before the biological treatment is effective for controling ozone dosage and enhancement of NOMs removal by biological processes.     

EVAPOTRANSPIRATION MEASUREMENT AND ESTIMATION OF THREE WETLAND ENVIRONMENTS IN THE UPPER ST. JOHNS RIVER BASIN,FLORIDA1

ABSTRACT: Accurate estimates of evapotranspiration from areas dominated by wetland vegetation are needed in the water budget of the Upper St. Johns River Basin. However, local data on evapotranspiration rates, especially in wetland environments, were lacking in the project area. In response to this need, the St. Johns River Water Management District collected evapotranspiration field data in Fort Drum Marsh Conservation Area over the period 1996 through 1999. Three large lysimeters were installed to measure the evapotranspiration from different wetland environments: sawgrass (Cladium jamaicense), cattail (Typha domingensis), and open water. In addition, pan evaporation was measured with a standard class “A” pan. Concurrently, meteorological data including rainfall, solar radiation, wind speed, relative humidity, air temperature, and atmospheric pressure were collected. By comparing computed evapotranspiration rates with those measured in the lysimeters, parameters in the Penman‐Monteith, the Priestley‐Taylor, and Reference‐ET methods, and evaporation pan coefficients were estimated for monthly and seasonal cycles. The results from the data collected in this study show that mean monthly evapotranspiration rates, computed by the different methods, are relatively close. From a practical point of view, results indicate that the evaporation pan can be used equally well as the more complex and data‐intensive methods. This paper presents the measured evapotranspiration rates, evaporation pan coefficients, and the estimated parameter values for three different methods to compute evapotranspiration in the project area. Since local data on evaporation are often scarce or lacking, this information may be useful to watershed hydrologists for practical application in other project regions.     

Enhanced decomposition of NO on the alkalized PdO/Al2O3 catalyst

Experimentally, decomposition of NO on the alkalized Pd/Al2O3 catalyst is remarkably enhanced at 825-1000 K. The enhancement in N2 yield may be due to the additional basic sites on the alkalized catalyst that can trap NO molecules. However, at T > 1000 K, due to the fact that the absorbed oxygen in subsurface or bulk of Pd was involved in the formation and desorption of oxygen molecules, yield of oxygen was enhanced.     

Volatilization of ketones

Volatilization fluxes of seven ketones were measured over a range of temperatures. Gas-film coefficients were calculated from these volatilization fluxes and related to the gas-film coefficient for the evaporation of water. These relations, when combined with an equation for estimating the gas-film coefficient for evaporation of water from a canal, permit estimating gas-film coefficients for the volatilization of ketones from streams and rivers.