Microbial response to repeated treatments of manure containing sulfadiazine and chlortetracycline in soil

Substantive addition of antibiotic-contaminated manure to agricultural soil may lead to “persistent” residues of antibiotics and may affect soil health. Therefore, this study examines the effects of repeated manure treatments containing sulfadiazine (SDZ) and chlortetracycline (CTC) residues, both individually and combined, on the functional diversity and structure of soil microbial communities in the soils under laboratory conditions. The average well color development (AWCD), Simpson diversity index (1/D, dominant populations), Shannon-Wiener diversity index (H′, richness), and McIntosh diversity index (U, evenness) in the antibiotics-treated soils decreased in the first 60-day treatment and then gradually recovered or even exceeded the initial level in the unamended soils with increasing treatment frequency. A total of 11 specific bands in temperature gradient gel electrophoresis (TGGE) profiles were observed and sequence analyzed for five repeated treatments, and most of them belonged to the phyla Firmicutes, Actinobacteria, and Proteobacteria. These results indicate that repeated treatments of manure containing SDZ and CTC residues can alter soil microbial community structure, although they have a temporary suppression effect on soil microbial functional diversity.     

Emergy Evaluation of a Village Ecosystem in Wolong Nature Reserve in China

Abstract

Due to historic reasons, there are several human settlements in nature reserves and national parks, which may constitute a threat to biodiversity. Establishment of nature reserves may however generate constraints for development of local communities residing in these reserves. The village ecosystem of Zhuanjinglou Village in Wolong Nature Reserve includes population, farming and livestock (domestic and alpine) and forest ecosystem. An emergy analysis of agro-ecosystem (including the farming and livestock ecosystem) and energy consumption in Zhuanjinglou Village indicates that livestock ecosystem plays an important role in village ecosystem, which may be the source of great pressure on the state of the local environment. The increasing ratio of electricity in energy consumption may play a positive role for environmental conservation. Despite some successful experiences, there are some remaining issues that need to be addressed in the ecological rehabilitation of the village ecosystem.     

低压放空天然气的高效再利用

为了提高试采放空天然气的利用率,考虑将不能输送的放空低压气进行技术增压,达到输送压力后,使其进入油气处理装置进行分离,其中一部分通过注气压缩机回注至地层,保持地层压力,提高油田凝析油采收率和延长油气田开发时间,实现低压气的高效利用。对站外试采井的低压放空气进行增压回收,减少了天然气资源浪费,实现了向资源节约型、环境保护企业的健康发展。     

Coping with the liquidity crisis: a new dynamic quota readjustment scheme for carbon markets

Environmental Geochemistry and Health - Because of insufficient liquidity, prices in the carbon market are more vulnerable to unexpected events, for which the impact duration lasts longer than that...     

集约化畜禽养殖对环境的污染及防治研究

治理集约化畜禽养殖废弃物污染,开展废弃物的资源化利用,是解决中国畜牧业环境污染问题的主要路径。文章详述了中国畜禽养殖业污染问题的产生及对环境可能造成的影响,分析了畜禽废弃物处理措施与资源化利用的主要方式及其效果和存在的问题,并提出了控制畜禽粪污染物的对策和环境管理建议,将有助于推动中国畜禽养殖业的可持续发展,改善农业生态环境及农村人居环境。     

Ancient paddy soils from the Neolithic age in China’s Yangtze River Delta

Identifying prehistoric irrigated rice fields and characterizing the beginning of paddy soil development are important for a better understanding of human development and agricultural history. In 2003, paddy soils and irrigated rice fields buried at a depth of 100–130 cm were excavated at Chuo-dun-shan in the Yangtze River Delta, close to Suzhou, China. The fields of sizes between 1.4 and 16 m² were surrounded with ridges that were connected to ditches/ponds via outlets to control the water level within the fields. Many carbonized and partly carbonized rice grains with an age of 3,903 B.C.(measured ¹⁴C age 5,129±45 a BP) were recovered. The surface layers of these buried paddy fields showed a high content of soil organic matter and a considerable high density of rice opals. The latter were identified to derive from Oryza spp. Solid-state ¹³C nuclear magnetic resonance spectroscopy revealed aromatic carbon (C) as the predominant organic C form in the fossil surface layer. This is expected, if the major source represents burnt rice and straw. In summary, our data are in agreement with new evidences indicating that in China, paddy soils and irrigated rice cultivation were initiated and developed more than 6,000 years ago.     

Bioaccumulation, depuration and oxidative stress in fish Carassius auratus under phenanthrene exposure

In this study, laboratory experiment was carried out to determine phenanthrene bioaccumulation, depuration in whole fish and oxidative stress in the liver of freshwater fish Carassius auratus. Fish were exposed to 0.05 mg/l phenanthrene for different periods, while one control group was designated for each exposure group. Some fish after 7 days of exposure were transferred to diluted water. The concentrations of phenanthrene in fish were analyzed by HPLC. Twenty-four hours after the exposure, reactive oxygen species (ROS) were trapped by phenyl-tert-butylnitrone and detected by electron paramagnetic resonance (EPR). The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione-s-transferase (GST) were also determined. The concentrations of phenanthrene in fish increased rapidly shortly after the start of the exposure, reached a maximum level at the 2 days, and then it declined quickly to low-level-steady state. The elimination process of phenanthrene could be divided into two periods-a fast elimination period following a slower loss period. The elimination curve could be fitted mathematically as the sum of two exponential functions according to two-compartment model: C(t)=2.72e(-1.065t) + 0.68e(-0.0364t). The PBN-radical adducts were detected in fish liver samples following the exposure 24h. The hyperfine splitting constants for the PBN-radical adducts were aN = 13.5 G, aH = 1.77 G and g value was 2.0058, which were consistent with those of PBN/()OH. The results indicated that the hydroxyl radical was probably significantly induced during the exposure of phenanthrene, as compared to the control group. The changes of activities of the antioxidant enzymes also were observed. In addition, after fish were removed from phenanthrene exposure, the recovery status of these antioxidant indices was explored. These results clearly indicated phenanthrene could be accumulated in fish and similar redox cyclings were produced, resulting in the changes of the activities of the antioxidant enzymes and the production of ROS with the oxidative stress.     

QSPR study on soil sorption coefficient for persistent organic pollutants

Quantitative structure-property relationship (QSPR) models of soil sorption coefficients for 32 persistent organic pollutants were constructed using our recently introduced Lu index and novel distance-based atom-type DAI topological indices. Using multiple linear regression technique, a 6-variable model was obtained with the correlation coefficient of estimations (R) being 0.95, and the standard error of estimations (s) being 0.23, and the correlation coefficient (R(cv)) and the standard error (s(cv)) in the leave-4-out cross-validation procedure are 0.90 and 0.31, respectively. The results in this study indicate that soil sorption coefficients of POPs are dominated by molecular size while some DAI indices have smaller influence.     

An integrated assessment model of carbon sequestration benefits: a case study of Liping county, China

This research attempts to model the complexity of planting trees to increase China's CO(2) sequestration potential by using a GIS-based integrated assessment (IA) approach. We use the IA model to assess the impact of China's Grain for Green reforestation and afforestation program on farmer and state incomes as well as CO(2) sequestration in Liping County, Guizhou Province. The IA model consists of five sub-models for carbon sequestration, crop income, timber income, Grain for Green, and carbon credits. It also includes a complementary qualitative module for assessing program impacts by gender and ethnicity. Using four scenarios with various assumptions about types of trees planted, crop incomes by township, CO(2) credit prices, state subsidies, methods for estimating carbon sequestered, and harvesting of trees, we find great variation in the impact of the Grain for Green program on incomes and on carbon sequestered over a 48 year period at both the county and township levels.     

HCO-3碱度对铜绿微囊藻生长与光合活性的影响

研究了23 mM (ALK23)和124 mM (ALK124) HCO-3对铜绿微囊藻(〖WTBX〗Microcystis aeruginosa〖WTBZ〗 FACHB 927)生长与光合特性的影响。实验结果表明，ALK23显著抑制铜绿微囊藻的生长，至培养结束，与对照相比对生物量的抑制率为38%，ALK124条件下虽然至培养结束生物量没有显著变化，但碱度增加铜绿微囊藻生物量有一个快速的增殖期，之后其生长速率持续下降。碱度增加强烈抑制光合色素Chla的合成，ALK23和ALK124条件下对Chla含量抑制率分别为74％和56％。藻胆蛋白与叶绿素a的比值（PBP/Chla）在碱度增加的条件下有显著升高。HCO-3碱度增加，铜绿微囊藻的光合活性先降低之后逐渐恢复，至处理结束表现出低碱度（ALK23）促进光合活性，高碱度（ALK124）对光合活性影响不显著。说明HCO-3碱度增加对铜绿微囊藻有一定伤害作用，与高碱度（ALK124）相比，低碱度（ALK23）对细胞的伤害程度更大，随着细胞的增殖，碱度对细胞的伤害作用逐渐恢复，且低碱度下细胞恢复更快。暗示碱度可以作为水华控制的手段之一.