Prenatal diagnosis of β thalassaemia by reverse phase HPLC

Reverse phase HPLC of radioactive globin chains has been compared to classical carboxy methyl cellulose chromatography for the prenatal diagnosis of β thalassaemia. The two methods correlated highly (r = 0.97 p < 0.0005) and provided an identical diagnosis for 40 fetal blood samples of fetuses homozygous or heterozygous for β thalassaemia. The HPLC procedure was much faster and required fewer biochemical steps (no globin preparation). It was at least as accurate and more sensitive than the classical chromatography. A single column can be used for 150 analyses and is always ready to be used. Last but not least it is much less expensive than CMC chromatography.     

Verification of carrier status for Becker muscular dystrophy from analysis of a blighted ovum

The polymerase chain reaction (PCR) was used on material from a blighted ovum to confirm indirectly the carrier status of a woman with a family history of Becker muscular dystrophy. Conventional testing including creatine kinase levels, muscle biopsy, and EMG had been inconclusive, and on the basis of one elevated creatine kinase level, the woman had been designated a possible carrier. Ultrasound examination at 10 weeks of pregnancy indicated a blighted ovum, from which DNA was subsequently extracted and subjected to PCR testing for determination of sex and genotypic status with respect to the known familial deletion of the dystrophin gene. The blighted ovum was found to have a Y chromosome and also to be deleted for at least exon 6 of the dystrophin gene, indirectly indicating that the mother most likely carried the family mutation for Becker muscular dystrophy.     

Prenatal sonographic assessment of early,rapidly growing fetal cervical teratoma

Although malignant transformation of fetal cervical teratoma is extremely rare, perinatal morbidity is high and usually related to the size of the tumour, which may compromise fetal swallowing and subsequently lead to upper airway obstruction. We present a case in which mid-trimester serial sonography demonstrated markedly rapid early growth of a lesion of this type between 17 and 19 weeks' gestation indicating the aggressive nature of this tumour, assisting parental decision to terminate the pregnancy. Histopathology confirmed grade 3 immaturity of the lesion.     

葫芦岛市生态环境现状及保护对策

介绍了葫芦岛市生态环境现状及主要问题，分析了各种生态问题产生的原因，阐述了保护和建设生态环境的主要对策。     

浅谈进口智能仪表在污水处理厂的使用和维护

对智能仪表的使用和维护进行了详细的介绍和讨论,指出使用智能仪表的优点及需要注意的具体事项并对仪表可能出现的问题进行了研究和探讨。     

热带假丝酵母8953菌株对苯酚的降解特性研究

实验室分离的一株热带假丝酵母(编号为8953)具有较强的苯酚降解能力,并可以利用苯酚、间苯二酚、联苯胺等芳烃为唯一碳源和能源生长。该菌株在48h内可完全降解约14.88mmol/L苯酚,完全降解10.63mmol/L苯酚只需要24h,其降解苯酚浓度最高可达19.13mmol/L。在pH4～9,温度20℃～40℃范围内,苯酚浓度为10.0mmol/L条件下该菌株均保持100%的降解率。该菌株降解苯酚的最适pH范围为6.0～8.0,最适温度范围为28℃～30℃,最佳接种量为1%～3%,外源添加氮源能促进酵母生长和苯酚的降解。     

Effect of long-term fertilization on soil nitrate distribution

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微生物固定化技术与载体结构的研究

分析微生物固定化的一般方法、常用固定化载体的特性 ;并通过实验研究固定化微生物颗粒处理微污染水的净水效能 ,分析载体结构对微生物固定化微生物活性的影响