黄颡鱼黑色素细胞原代培养及迁移相关基因克隆分析

黑素皮质素受体-1(MC1R)在鱼体中对黑色素细胞的分化及迁移起主要的调控作用,而且在神经系统和免疫系统中表现出不同功能。本研究对黄颡鱼表皮黑色素细胞的培养条件进行探索,并在此基础上对MC1R基因进行克隆,为后续研究黄颡鱼黑色素异常的细胞及分子机理研究提供基础。结果表明,黄颡鱼表皮黑色素细胞在27 ℃培养条件下,72 h开始贴壁生长,培养基中添加20 %小牛血清培养效果比添加10%小牛血清好。MC1R基因克隆方面,黄颡鱼MC1R基因全长为936 bp,编码312个氨基酸,与剑尾鱼(Xiphophorus maculates)、孔雀鱼(Poecilia reticulata)、条斑星鲽(Verasper moseri)、海鲈(Dicentrarchus labrax)、大菱鲆(Psetta maxima)等5种鱼类MC1R基因的同源性分别为97%、96%、90%、90%、90%,系统分析结果显示,黄颡鱼MC1R基因在进化树上的位置与黄颡鱼的分类所处位置基本吻合。证明黄颡鱼表皮黑色素细胞原代培养的条件为：27℃,添加20% 小牛血清,所克隆基因为黄颡鱼MC1R基因。     

环保标准对环保产业的影响分析

环保标准是实施国家环境保护法律、法规的技术依据, 是开展环境管理、环境执法工作的有力抓手。环 保产业是促进清洁生产和污染减排,实现达标排放的有效措施。环保标准对环保产业的发展具有重要影 响,是环保产业发展的动力,为环保产业挖掘潜能、拓宽空间、提供指导,同时又与环保产业相互依 存。强化环保标准对环保产业的促进作用,应以改革创新的精神综合考虑当前面临的主要问题,有序推 进环保标准的制定和实施工作。     

Microbial diversity in solar greenhouse soils in Round-Bohai Bay-Region,China: The influence of cultivation year and environmental condition

Environmental Science and Pollution Research - Round-Bohai Bay (RBB)-Region is an important crop production area in China, where vegetables are mainly produced in solar greenhouses. However,...     

污泥、皂素抽提物(纤维渣)作为花卉培养基质的实验研究

利用污水处理厂的生化污泥、皂素抽余物为主要原料,配合珍珠岩进行改性和调理,研发出一种新型的花卉栽培基质,解决黄姜皂素生产中产生的固体废弃物的污染问题,并为污泥应用于园林提供参考和依据.结果表明:污泥、纤维渣为主要成分的混合基质替代泥炭是完全可行的,最佳配比为处理4(污泥:纤维渣:珍珠岩=50%:30%:20%)和处理5(污泥:纤维渣:珍珠岩=60%:20%:20%).     

Molecular cloning, characterization of CAT, and eco-toxicological effects of dietary zinc oxide on antioxidant enzymes in Eisenia fetida

The full-length cDNA of catalase (EfCAT) from Eisenia fetida was cloned (GenBank accession no. JN617999). Sequence characterization revealed that EfCAT protein sequence contained proximal heme-ligand signature sequence (³⁵¹RLFSYSDTH³⁵⁹), two glycosylation sites (N₁₄₅ and N₄₃₆), the proximal active site signature (⁶¹FDRERIPERVVHAKGAGA⁷⁸), and 12 amino acids (N₁₄₅, H₁₉₁, F₁₉₅, S₁₉₈, R₂₀₀, N₂₁₀, Y₂₁₂, K₂₃₄, I₂₉₉, W₃₀₀, Q₃₀₂, and Y₃₅₅), which were identified as putative residues involved in NADPH binding. These conserved motifs and catalase signature sequences were essential for the structure and function of EfCAT. The present study also investigated the effect of the veterinary food additive zinc oxide on antioxidant processes in E. fetida, at different concentrations and exposure durations. A significant increase (by 106.0 % compared to controls) in CAT activity at 500 mg/kg was registered at day 15. The superoxide dismutase (SOD) activity at 500 mg/kg increased to the maximum value (by 44.0 %) measured at day 15. There was a significant increase in glutathione peroxidase (GPx) activity for all concentrations after 5 days. The results showed that dietary Zn (500 mg/kg) causes oxidative damage to earthworms. At early stages of earthworms exposed to ZnO, GPx is the main enzyme to impair the oxidative status; while at later stages the enzymes CAT and SOD were the main indicators of oxidative stress. The antioxidant enzymatic variations may be an adaptive response of earthworms to survive in contaminated soils.     

Target level of safety for Chinese airspace

This article considers the target level of safety (TLS) for Chinese airspace. If collision risk is lower than TLS, we can say the separation minima meet the safety requirements, so the TLS is the safety limit on the flight operation. But there are no regulations on TLS in China by now, hence, it is necessary to judge whether it is fair to apply the TLS proposed by ICAO to evaluating air traffic safety in China.The accidents data of more than 50 years are collected, and we apply the trend extrapolation to predicting the safety level in the future so as to resolve the TLS problem. The resulted showed the safety level in the period from 2010 to 2020 is very close to TLS established by ICAO. The results show the safety level of collision risk in China has reached the international level and it is rational to use TLS of ICAO to assess collision risk.