Analysis of microcystins in cyanobacteria blooms and surface water samples from Meiliang Bay,Taihu Lake,China

Taihu Lake is the third largest freshwater lake in China. In recent years, the water pollution of cyanobacteria blooms has become a severe problem in this area. Microcystins (MCs) are an important group of toxic compounds mainly produced by some cyanobacteria species and have both acute and chronic hepatotoxic effects on animals and humans. This paper presents the first data on the identification and detection of MCs in both natural occurring cyanobacteria blooms and surface water samples (0-0.5 m), collected from Meiliang Bay, Taihu Lake, China. A conventional method for extraction and isolation of MCs from cyanobacteria blooms was applied. High-performance liquid chromatography (HPLC) analysis showed that the main toxic component in the cyanobacteria materials was MC-LR. The monoclonal antibody (mAb) against MC-LR produced by hybridoma technique was employed for direct competitive ELISA to detect the concentrations of MCs in bloom and water samples collected in 2001. The results not only revealed the presence of MCs but also temporal variations of MCs levels of three sampling stations in Meiliang Bay in 1 year. It is obvious that the MC contents were relatively higher during warm months and related with the status of eutrophication. Our study indicates the threat associated with MCs in water body of Taihu Lake. To prevent the MCs potential hazard on public health in this area, some necessary measures of monitoring and control of growth of cyanobacteria are urgently needed.     

基于AVHRR的成都平原城市热岛效应演变趋势分析

作为城市气候主要特征之一的热岛效应有日渐明显的趋势，给城市发展和市民生活带来一系列负面影响。对2000～2006年春季下午NOAA气象卫星遥感数据的处理和分析发现，随着城市的扩张，成都平原城市热岛效应的规模呈扩大趋势。成都市区热岛强度一般为5℃-7℃，局部地方极值可达8℃以上；市中心区热岛强度相对减弱，东部工业区热岛高温区面积逐渐缩小，市区西南部热岛明显发展，致使2003年起热岛次高温区在二、三环路附近大致呈环状分布。热岛演变与近几年城市建设和改造活动密切相关。研究成果为政府相关部门缓解热岛效应、改善人居环境提供了参考依据。     

高职院校食品专业《食品微生物学》考试改革初探

针对食品微生物学的课程考核方式进行了初步研究，采取对照试验的方法，对传统的期末考试方法与“当堂课考核需要掌握的关键点”的新方法进行了比较。食品微生物学课程的理论部分和实验部分采用“当堂课考核需要掌握的关键点”的考试方法后，不仅大大提高了学生学习本课程的积极性，而且还提高了学习成绩，特别是学生对“必需掌握的关键点”掌握得特别牢固。     

输变电项目环评存在问题分析及对策建议

列举了国家环保总局环境工程评估中心在输变电项目环境影响技术评估中遇到的一些技术和管理上亟待解决的问题,在对其分析的基础上,提出相应的对策建议.     

北京山区公路地质灾害应急与防治对策

北京是地质灾害较严重的城市之一,具有灾种多、活动频繁、群发性强的特征。北京山区公路所遭受的地质灾害主要有滑坡、滑(崩)塌、泥石流、岩溶、采空地面塌陷等。为了防止山区公路地质灾害可能造成的危害,笔者通过对北京山区公路所遭受的地质灾害类型、发育特征、分布规律等特点及其所造成的危害进行分析、研究,提出了山区公路地质灾害应急与防治对策,以提高北京市的防灾减灾能力,减少和避免地质灾害造成的损失。     

地裂缝上框架结构的抗震性能分析

地裂缝是严重影响我国人类生活、生产建设的主要地质灾害之一,是一种独特的地质灾害,近年来已引起人们的关注,它广泛分布于全国许多地方。框架结构是一种广泛使用的结构形式。由于人类活动或地质构造运动形成地裂缝,进而造成房屋开裂或倾斜。该文针对位于抗震设防区的框架结构房屋,分别用底部剪力法和PKPM静力分析法,来定量研究在地裂缝影响下框架结构的抗震性能,为双重灾害影响下结构抗震鉴定加固提供依据。     

填料在自由水面人工湿地中的应用

本文通过对深圳市某橡胶厂自行设计造的自由水面人工湿地的调查研究，分析了在人工湿地中采用填料代替植物的工作特性，并论证了土壤－植物－微笑一这样一个生态系统是传统自由水面湿地处理系统实现其处理污水功能的核心。     

Role of nitric oxide in the genotoxic response to chronic microcystin-LR exposure in human–hamster hybrid cells

Microcystin-LR(MC-LR) is the most abundant and toxic microcystin congener and has been classified as a potential human carcinogen(Group 2B) by the International Agency for Research on Cancer. However, the mechanisms underlying the genotoxic effects of MC-LR during chronic exposure are still poorly understood. In the present study, human–hamster hybrid(AL) cells were exposed to MC-LR for varying lengths of time to investigate the role of nitrogen radicals in MC-LR-induced genotoxicity. The mutagenic potential at the CD59 locus was more than 2-fold higher(p 0.01) in ALcells exposed to a cytotoxic concentration(1 μmol/L) of MC-LR for 30 days than in untreated control cells, which was consistent with the formation of micronucleus. MC-LR caused a dose-dependent increase in nitric oxide(NO) production in treated cells. Moreover, this was blocked by concurrent treatment with the NO synthase inhibitor NG-methyl-L-arginine(L-NMMA), which suppressed MC-LRinduced mutations as well. The survival of mitochondrial DNA-depleted(ρ0) ALcells was markedly decreased by MC-LR treatment compared to that in ALcells, while the CD59 mutant fraction was unaltered. These results provided clear evidence that the genotoxicity associated with chronic MC-LR exposure in mammalian cells was mediated by NO and might be considered as a basis for the development of therapeutics that prevent carcinogenesis.     

科尔沁沙地生态系统服务价值变化研究

研究土地利用变化引起的区域生态系统服务价值的改变。可定量评价土地利用活动对区域生态环境的影响。基于1995年和2000年的土地利用数据．应用谢高地等人制定的中国陆地生态系统单位面积服务价值表．估算了科尔沁沙地土地利用变化引起的生态系统服务价值的改变。结果表明。从1995—2000年5年间。科尔沁沙地的耕地增加4．87％。林地减少2．25％。草地减少0．03％．水域减少1．32％．湿地增加0．54％，城乡工矿居民用地减少3．21％。未利用地减少8．83％；研究区内土地利用变化的区域差异性很大．表现为大部分旗县仍维持着毁林毁草开荒的不合理土地利用活动。科尔沁沙地生态系统服务价值从1995年的1463．67亿元增加到2000年的1467．36亿元。净增加值为3．69亿元，增幅为0．25％；其增加值并非因耕地面积增大所产生．而是个别旗县的大面积沙荒地得到有效治理所致。