The effect of titanium dioxide nanoparticles on neuroinflammation response in rat brain

Titanium dioxide nanoparticles (TiO₂ NPs) are widely used for their whiteness and opacity in several applications such as food colorants, drug additives, biomedical ceramic, and implanted biomaterials. Research on the neurobiological response to orally administered TiO₂ NPs is still limited. In our study, we investigate the effects of anatase TiO₂ NPs on the brain of Wistar rats after oral intake. After daily intragastric administration of anatase TiO₂ NPs (5–10 nm) at 0, 50, 100, and 200 mg/kg body weight (BW) for 60 days, the coefficient of the brain, acethylcholinesterase (AChE) activities, the level of interleukin 6 (IL-6), and the expression of glial fibrillary acidic protein (GFAP) were assessed to quantify the brain damage. The results showed that high-dose anatase TiO₂ NPs could induce a downregulated level of AChE activities and showed an increase in plasmatic IL-6 level as compared to the control group accompanied by a dose-dependent decrease inter-doses, associated to an increase in the cerebral IL-6 level as a response to a local inflammation in brain. Furthermore, we observed elevated levels of immunoreactivity to GFAP in rat cerebral cortex. We concluded that oral intake of anatase TiO₂ NPs can induce neuroinflammation and could be neurotoxic and hazardous to health.     

Bioremediation of kerosene I: A case study in liquid media

The ability of different local isolates in addition to some isolates from Germany to degrade kerosene in liquid medium was studied. The results showed that the percent of kerosene degradation varied among the different organisms and that 59–94% of kerosene was degraded after 21 d. Two local isolates (Pseudomonas sp. AP and Pseudomonas sp. CK) and one German isolate (Gordonia sp. DM) were selected for this study. The addition of wheat bran, as co-substrate, stimulated the kerosene degradation by the two local strains, while glucose inhibited the degradation rate using the three organisms with different rates. Ammonium nitrate and urea was the best nitrogen sources. The use of superphosphate (as phosphorus source) in the presence of urea stimulates the degradation rate. It was also observed that the addition of 1% surfactants, like Triton X-100, Igepal, Tergitol, or Tween 20 and 80 enhanced the kerosene degradation. The degradation percent lied between 94% and 98%. The ability of the tested organisms to degrade kerosene concentration from 2% to 8% was evaluated. It was found that the three organisms degraded about 65–85% from 8% kerosene after 21 d. The use of rice straw-immobilized cells reduced the time of degradation and enhanced the degradation ability of the organisms. The sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed the presence of a common protein band when the tested organisms were grown on kerosene.     

Microwave-assisted synthesis of biodegradable and antibacterial thiophene,furan and thiazole derivatives

Environmental Chemistry Letters - The rise of bacterial resistance to common clinical antibiotics is calling for alternative techniques to synthesize antibacterial drugs with high biodegradability....     

Biosorption of copper from aqueous solutions by date stones and palm-trees waste

The removal of toxic metals from wastewaters by biosorption, based on the metal-binding capacities of various biological materials, has received much interest. However, the success of this approach depends on economic feasibility, which can be obtained by optimization of the environmental conditions. This paper evaluates, for the first time, the use of low-cost biosorbent (date stones (DS) and palm-tree waste (PTW)) to eliminate Cu(II) from aqueous solutions. The effect of some parameters on copper biosorption has been studied using date stones and palm-tree waste as solid sorbents. Results show that the highest percentage of copper adsorption was obtained for the smallest size of the sorbent particles. The biosorption process was found to occur rapidly, i.e. the maximum sorption capacity was reached within 20 min. The process involved pseudo-second-order kinetics with an activation energy value within the normal range considered for processes, where a physical interaction between the sorbate and the sorbent solid predominated. The thermodynamic parameters of the copper ions uptake onto the solid sorbents indicated that, the process was endothermic and proceeds spontaneously from the date stones. However, the thermodynamic studies of the adsorption of copper on palm-tree waste indicated that the process was exothermic and proceeds spontaneously.     

Synthesis and evaluation as biodegradable herbicides of halogenated analogs of L-meta-tyrosine

l-meta-tyrosine is an herbicidal nonprotein amino acid isolated some years ago from fine fescue grasses and characterized by its almost immediate microbial degradation in soil (half-life <24 h). Nine monohalogenated or dihalogenated analogs of this allelochemical have been obtained through a seven-step stereoselective synthesis from commercial halogenated phenols. Bioassays showed a large range of biological responses, from a growth root inhibition of lettuce seedling similar to that noted with m-tyrosine [2-amino-3-(2-chloro-5-hydroxyphenyl)propanoic acid or compound 8b] to an increase of the primary root growth concomitant with a delay of secondary root initiation [2-amino-3-[2-fluoro-5-hydroxy-3-(trifluoromethyl)phenyl]propanoic acid or compound 8h]. Compound 8b was slightly less degraded than m-tyrosine in the nonsterilized nutritive solution used for lettuce development, while the concentration of compound 8h remained unchanged for at least 2 weeks. These data indicate that it is possible to manipulate both biological properties and degradation of m-tyrosine by halogen addition.     

Metals bioaccumulation and histopathological biomarkers in Carcinus maenas crab from Bizerta lagoon,Tunisia

Metals concentrations and histolopathological lesions of gills and digestive gland were investigated in Carcinus maenas crabs sampled from Bizerta Lagoon and Kuriat Island (Tunisia) as control site. The concentrations of trace metals varied between tissues, sites and sampling time. The highest levels of the analysed metals in gills and digestive gland were noted in Menzel Bourguiba and Cimentery sites at both sampling times (February and July). The higher metals loads were associated with severe and various tissues alterations in contaminated crabs. We particularly noted in the gills a haemocytic infiltration, distension and enlargement of the lamellae, lifting of lamellar epithelium, necrotic lesions and fusion of lamellae in the most polluted sites (Menzel Bourguiba and Cimentery). Moreover, others pathological alterations were observed in digestive gland of crabs collected from polluted sites and with a severity site dependent. We observed necrotic tubules containing tissue debris in the lumen with more intensity in crabs collected from Cimentery site in both sampling times. The thickened basal laminae and the walling off of the tubules by haemocytes around the thickened basal laminae were more abundant at Menzel Bourguiba than at others sites. The coagulation in the thickened basal laminae was observed only at Cimentery in February. Tissues histopathological lesions were sensitive to discriminate crabs of different sites and demonstrated its usefulness in this biomonitoring study. We recommend the association of histopatholocial lesions to biochemical biomarkers in future biomonitoring studies.     

Sodium arsenate induce changes in fatty acids profiles and oxidative damage in kidney of rats

Six groups of rats (n?=?10 per group) were exposed to 1 and 10 mg/l of sodium arsenate for 45 and 90 days. Kidneys from treated groups exposed to arsenic showed higher levels of trans isomers of oleic and linoleic acids as trans C181n-9, trans C18:1n-11, and trans C18:2n-6 isomers. However, a significant decrease in eicosenoic (C20:1n-9) and arachidonic (C20:4n-6) acids were observed in treated rats. Moreover, the “Δ5 desaturase index” and the saturated/polyunsaturated fatty acids ratio were increased. There was a significant increase in the level of malondialdehyde at 10 mg/l of treatment and in the amount of conjugated dienes after 90 days (p?p?p?p?n-6 polyunsaturated fatty acids and leads to an increase in the trans FAs isomers. Therefore, FA-induced arsenate kidney damage could contribute to trigger kidney cancer.     

Uranium isotopes in groundwater from the “Jeffara coastal aquifer” (southeastern Tunisia)

The distributions of ²³⁸U and ²³⁴U in groundwater from the “Jeffara aquifer” were studied by using alpha spectrometric methods. The concentration ranges of ²³⁸U and ²³⁴U/²³⁸U activity ratios were 1.34 ± 0.17 to 3.43 ± 0.38 ppb, and 1.43 ± 0.23 to 1.82 ± 0.27 respectively. Variations in concentrations can be related not only to lithostratigraphic formations but also to different origins of groundwater. U content of Jeffara are found very similar to those of Continental Intercalaire aquifer in both El Hamma and Chenchou regions, indicating that the Continental Intercalaire is the dominant source of the groundwater.