Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation

Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Pančevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography—mass spectrometry (GC–MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.     

Sources of organochlorine pesticides in air in an urban Mediterranean environment: volatilisation from soil

Organochlorine pesticide (OCP) cycling was studied in the area of Banja Luka, Bosnia and Herzegovina, over 3 days in summer with high temporal (4 h-means) and spatial (3 sites distanced 3-6 km) resolutions. Elevated levels of DDX compounds (i.e. o,p'- and p,p'-isomers of DDT, DDE and DDD, 44-74 pg m(-3) at the urban sites and 27 pg m(-3) as a background level), HCH (α-, β- and γ-isomers, 52-70 vs. 147 pg m(-3)), HCB (34-48 vs. <0.1 pg m(-3)) and pentachlorobenzene (6.8-9.9 vs. 6.0 pg m(-3)) were found. The variation of OCP levels at the two urban sites was not in phase, except for most DDX compounds. This was related to background levels, which for HCH were higher than in the urban area. Vertical profiles between samples collected from 1.1 and 2.3 m (part of the time 0.6 and 2.3 m) above a soil, which was only moderately contaminated by OCPs (0.12 ng g(-1) HCH, 0.11 ng g(-1) DDX, 0.44 ng g(-1) HCB) were analysed. Volatilisation from the ground caused negative vertical concentration gradients of HCH isomers (day and night), but not for HCB (except for 1 day-time sample) and DDX compounds (except p,p'-DDD, day-time, sporadically). The concentration in air and the vertical concentration gradient of the HCH isomers varied with air temperature (day-time maxima), while the variation of the HCB concentration was inversely related to air temperature and was determined by mixing (night-time maxima). α- and β-HCH were volatilised from soil throughout the three days, even during periods of cooling. Fugacity calculations, based on the absorption in soil organic matter as the process determining retention in soil, underestimated the volatilisation of β-HCH and p,p'-DDD. It is concluded that the representativeness of point measurements of OCPs in urban areas is limited by the spatial variability of soil contamination.     

Pollution of soils with organophosphorus flame retardants and plasticizers

The detection of the three organophosphate esters TCEP, TCPP and TBEP in soil samples indicates that pollution of soils from diffuse atmospheric sources has to be considered in risk assessments.     

Pollution status and health risk caused by heavy elements in the flooded soil and vegetables from typical agricultural region in Vojvodina Province,Serbia

Environmental Science and Pollution Research - The investigation conducted in the Vojvodina Province, as a typical European and one of the biggest agricultural regions in the Balkans, offers the...     

Evaluation of potential human health risks from exposure to volatile organic compounds in contaminated urban groundwater in the Sava river aquifer,Belgrade, Serbia

Environmental Geochemistry and Health - The oil pollutant in the Sava River aquifer in the residential area of Belgrade, Serbia was investigated in order to analyze the extent, origin and spatial...     

Radiological risks from 40 K, 226Ra and 232Th in urbanised and industrialised karstic coastal area (Kaštela Bay,Croatia)

Environmental Science and Pollution Research - Radiological risks associated with 40&nbsp;K, 226Ra and 232Th massic activities in limestones, marls, stream sediments and soils of the...     

Mapping of environmental and technological performance of food and beverage sector in Bosnia and Herzegovina

The objective of this paper is to discuss the mapping methodology implemented in the selection of BAT candidates and to present the results of the mapping of B&H food and beverage sector, prepared as a baseline for the development of national reference documents on Best Available Techniques. The subsectors subject to analysis were: dairy, fish farming, fish processing, fruit and vegetable processing, meat processing, breweries, and slaughterhouses.The sector was mapped from several aspects, including socio-economic, legal, and environmental point of view. The socio-economic aspect focused on understanding the sector’s structure and financial capabilities, pricing for water, energy, and solid waste consumption, as well as employee profiles, in order to better assess the sustainability of decisions taken. Legal mapping focused on relevant regulations, including policies from the following sectors: environment, water use and protection, food safety, agriculture, and veterinary, in order to determine legal requirements from an environmental protection aspect set in the various sector policies. Environmental performance indicators were calculated in order to assess present resource efficiencies and relevant environmental impacts. In addition, the mapping process also addressed the existing level of BAT implementation in the subsectors under consideration.The paper presents a rationale for methodology selection, offers a review of the mapping methodology used, discusses mapping results and provides conclusions in regards to that methodology used and discusses issues that need to be considered in the selection of BAT candidates.     

Implications of oxidative stress in occupational exposure to lead on a cellular level

The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.

Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu–Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.     

The application of reliability models in traffic accident frequency analysis

Analysis of traffic accident frequency represents an important subject of research of many authors. From the aspect of temporal analysis of traffic accident occurrence, two approaches have been singled out in previous practice: the collective (analyzes traffic accidents over a longer period of time) and individual (analyzes traffic accidents in real time). The paper shows that the system reliability theory, with certain adjustments, can be largely used to analyze traffic accident frequency based on the individual approach. A certain similarity has been observed between the system reliability theory and the traffic safety theory, and conceptual adjustment of equivalent terms and states has been performed based on this. A model has been successfully tested on the basis of which, for the road and sections, we have determined the traffic accident frequency, the probability of the occurrence of a certain number of traffic accidents and the mean time between two consecutive traffic accidents.