Mitigation of agricultural nonpoint-source pesticide pollution in artificial wetland ecosystems

Contamination caused by pesticides in agriculture is a source of environmental poor water quality in some of the European Union countries. Without treatment or targeted mitigation, this pollution is diffused in the environment. Pesticides and some metabolites are of increasing concern because of their potential impacts on the environment, wildlife and human health. Within the context of the European Union (EU) water framework directive context to promote low pesticide-input farming and best management practices, the EU LIFE project ArtWET assessed the efficiency of ecological bioengineering methods using different artificial wetland (AW) prototypes throughout Europe. We optimized physical and biological processes to mitigate agricultural nonpoint-source pesticide pollution in artificial wetland ecosystems. Mitigation solutions were implemented at full-scale demonstration and experimental sites. We tested various bioremediation methods at seven experimental sites. These sites involved (1) experimental prototypes, such as vegetated ditches, a forest microcosm and 12 wetland mesocosms, and (2) demonstration prototypes: vegetated ditches, three detention ponds enhanced with technology of constructed wetlands, an outdoor bioreactor and a biomassbed. This set up provides a variety of hydrologic conditions, with some systems permanently flooded and others temporarily flooded. It also allowed to study the processes both in field and controlled conditions. In order to compare the efficiency of the wetlands, mass balances at the inlet and outlet of the artificial wetland will be used, taking into account the partition of the studied compound in water, sediments, plants, and suspended solids. The literature background necessary to harmonize the interdisciplinary work is reviewed here and the theoretical framework regarding pesticide removal mechanisms in artificial wetland is discussed. The development and the implementation of innovative approaches concerning various water quality sampling strategies for pesticide load estimates during flood, specific biological endpoints, innovative bioprocess applied to herbicide and copper mitigation to enhance the pesticide retention time within the artificial wetland, fate and transport using a 2D mixed hybrid finite element model are introduced. These future results will be useful to optimize hydraulic functioning, e.g., pesticide resident time, and biogeochemical conditions, e.g., dissipation, inside the artificial wetlands. Hydraulic retention times are generally too low to allow an optimized adsorption on sediment and organic materials accumulated in artificial wetlands. Absorption by plants is not either effective. The control of the hydraulic design and the use of adsorbing materials can be useful to increase the pesticides residence time and the contact between pesticides and biocatalyzers. Pesticide fluxes can be reduced by 50–80% when hydraulic pathways in artificial wetlands are optimized by increasing ten times the retention time, by recirculation of water, and by deceleration of the flow. Thus, using a bioremediation method should lead to an almost complete disappearance of pesticides pollution. To retain and treat the agricultural nonpoint-source po a major stake for a sustainable development.     

Phoenix flagships: Conservation values and guanaco reintroduction in an anthropogenic landscape

Multiple forms of valuation contribute to public acceptance of conservation projects. Here, we consider how esthetic, intrinsic, and utilitarian values contribute to public attitudes toward a proposed reintroduction of guanaco (Lama guanicoe) in a silvopastoral system of central Chile. The nexus among landscape perceptions and valuations, support for reintroductions, and management of anthropogenic habitats is of increasing interest due to the proliferation of conservation approaches combining some or all of these elements, including rewilding and reconciliation ecology, for example. We assessed attitudes and values through an online questionnaire for residents of Santiago, Chile, using multiple methods including photo-montages and Likert scale assessments of value-based statements. We also combined the questionnaire approach with key informant interviews. We find strong support for the reintroduction of guanacos into the Chilean silvopastoral system (‘espinal’) in terms of esthetic and intrinsic values but less in terms of utilitarian values. Respondents preferred a scenario of espinal with guanacos and expressed interest in visiting it, as well as support for the reintroduction project on the basis that guanacos are native to central Chile. We suggest that reintroduced guanacos could serve as a ‘phoenix flagship species’ for espinal conservation, that is, a flagship species that has gone regionally extinct and is known but not associated with the region in the cultural memory. We consider how the lack of local cultural identity can both help and weaken phoenix flagships, which we expect to become more common.     

Atmospheric deposition may affect northern hardwood forest composition by altering soil nutrient supply.

In the northeastern United States, the input of reactive nitrogen (N) via atmospheric deposition has increased rapidly since the onset of the industrial revolution. During the same period of time, acid precipitation and forest harvest have removed substantial quantities of base cations from soil. Because of the dominance of base-poor soils and the low rates of atmospheric base cation deposition, soils throughout the northeastern United States may be increasingly rich in N but poor in calcium (Ca). We studied the consequences of a change in soil N and Ca availability on forest composition by transplanting seedlings of four tree species into replicate plots in the understory and in canopy gaps amended with N and Ca in factorial combination. In this paper, we report on the growth and survivorship of seedlings over a four-year period. Relative to control plots, fertilization with N increased red maple growth by an average of 39% whereas fertilization with Ca decreased survivorship in the understory by 41%. In sugar maple, fertilization with Ca increased growth by 232% and 46% in the forest understory and in canopy gaps, respectively, and significantly increased high light survivorship. Fertilization with N decreased white pine survivorship by 69% in the understory whereas high Ca availability significantly increased survivorship. Fertilization with N or Ca alone reduced red oak growth but had no effect on survivorship. The results of this study suggest that historical losses of soil Ca and the continuing effects of atmospheric-N deposition on N availability are likely to alter the composition of northeastern North American forests because of the positive effects of N enrichment on the growth of red maple and the negative effects of Ca loss on the growth and survivorship of sugar maple and white pine.     

The Effect of Heat on the Physicochemical Properties of Bacteriophage MS2

The differences in physicochemical characteristics between infectious and non-infectious viral particles are poorly known. Even for heat, which is known as one of the most efficient treatments to inactivate enteric viruses, the global inactivation mechanisms have not been described yet. Such knowledge would help distinguish between both types of particles and therefore clarify the interpretation of the presence of viral genomes in food after heat treatment. In this study, we examined in particular the differences in electrostatic charge and hydrophobicity between the two particle types. MS2 phage, a common surrogate for enteric viruses, was used as a model virus. The heat-induced inactivation process of the infectious phages caused hydrophobic domains to be transiently exposed and their charge to become less negative. The particles also became progressively permeable to small molecules such as SYPRO Orange dye. The presence of non-infectious phage particles in which the genome was not accessible to RNases has been clearly demonstrated. These observations were done for MS2 phages exposed to a temperature of 60 °C. When exposed to a temperature higher than their critical temperature (72 °C), the particles were disrupted and the genome became available for RNases. At lower temperatures, 60 °C in this study, the transient expression of hydrophobic domains of remaining infectious phages appeared as an interesting parameter for improving their specific detection.     

Increasing plant use of organic nitrogen with elevation is reflected in nitrogen uptake rates and ecosystem delta15N

It is hypothesized that decreasing mean annual temperature and rates of nitrogen (N) cycling causes plants to switch from inorganic to organic forms of N as the primary mode of N nutrition. To test this hypothesis, we conducted field experiments and collected natural-abundance delta15N signatures of foliage, soils, and ectomycorrhizal sporocarps along a steep elevation-climate gradient in the White Mountains, New Hampshire, USA. Here we show that with increasing elevation organic forms of N became the dominant source of N taken up by hardwood and coniferous tree species based on dual-labeled glycine uptake analysis, an important confirmation of an emerging theory for the biogeochemistry of the N cycle. Variation in natural abundance foliar delta15N with elevation was also consistent with increasing organic N uptake, though a simple, mass balance model demonstrated that the uptake of delta15N depleted inorganic N, rather than fractionation upon transfer of N from mycorrhizal fungi, best explains variations in foliar delta15N with elevation.     

An empirical perspective for understanding climate change impacts in Switzerland

Regional Environmental Change - Planning for the future requires a detailed understanding of how climate change affects a wide range of systems at spatial scales that are relevant to humans....     

Adsorption distribution impact on preferential transport within horizontal flow constructed wetland (HFCW)

Predicting preferential water flow and solute transport in soils is in the interest of scientists and engineers in the fields of agricultural soil, forest hydrology, soil physics and wastewater treatment by constructed wetland. In artificial wetlands, any preferential pathway induces an insufficient residence time of pollutants in the soil, making an incomplete and unfinished biodegradation process, a wrong evaluation of the hydraulic residence time of the system which would hinder its management in a complete system with several entities of treatment and a non-homogeneous growth of the biofilm in the solid filter mass. This paper is a contribution in tracer experiment data analysis within a horizontal flow constructed wetland built in a storm water basin. A two-dimensional numerical model was used to simulate flow and reactive solute transport. The flow model was successfully calibrated in very dry soil conditions. The adsorption profiles used in the reactive transport modeling are those of five molecules: metolachlor, atrazine, deethylatrazine (DEA), deisopropylatrazine (DIA), and hydroxyatrazine (HA). We show that the adsorption distribution is an internal factor of soil which is responsible to the “preferential” pathway transport in a homogeneous gravel texture. The mean residence time of pollutants within the filter is strongly correlated with the average value of the adsorption coefficient. Moreover, we note a lack of significant impact of the heterogeneity of the medium on the statistical moments of breakthrough curve. It appears that a uni-modal breakthrough curve is not significant to the absence of preferential flow in the medium and at least a two-dimensional display can provide sufficient evidence on the presence or absence of preferential pathways. Finally, using a PLS regression, it is possible to perfectly discriminate the number of peaks of concentration and the asymmetry of the breakthrough curve.     

Substrate supply, fine roots, and temperature control proteolytic enzyme activity in temperate forest soils

Temperature and substrate availability constrain the activity of the extracellular enzymes that decompose and release nutrients from soil organic matter (SOM). Proteolytic enzymes are the primary class of enzymes involved in the depolymerization of nitrogen (N) from proteinaceous components of SOM, and their activity affects the rate of N cycling in forest soils. The objectives of this study were to determine whether and how temperature and substrate availability affect the activity of proteolytic enzymes in temperate forest soils, and whether the activity of proteolytic enzymes and other enzymes involved in the acquisition of N (i.e., chitinolytic and ligninolytic enzymes) differs between trees species that form associations with either ectomycorrhizal or arbuscular mycorrhizal fungi. Temperature limitation of proteolytic enzyme activity was observed only early in the growing season when soil temperatures in the field were near 4 degrees C. Substrate limitation to proteolytic activity persisted well into the growing season. Ligninolytic enzyme activity was higher in soils dominated by ectomycorrhizal associated tree species. In contrast, the activity of proteolytic and chitinolytic enzymes did not differ, but there were differences between mycorrhizal association in the control of roots on enzyme activity. Roots of ectomycorrhizal species but not those of arbuscular mycorrhizal species exerted significant control over proteolytic, chitinolytic, and ligninolytic enzyme activity; the absence of ectomycorrhizal fine roots reduced the activity of all three enzymes. These results suggest that climate warming in the absence of increases in substrate availability may have a modest effect on soil-N cycling, and that global changes that alter belowground carbon allocation by trees are likely to have a larger effect on nitrogen cycling in stands dominated by ectomycorrhizal fungi.     

色谱自动进样瓶可溶出砷对样品砷形态测定的影响

选用几种常用玻璃色谱自动进样瓶(简称色谱瓶),包括硼硅玻璃、一级水解级玻璃和高白料玻璃色谱瓶,以及聚丙烯材质的色谱瓶,1%HNO3对色谱瓶浸提后用ICP-MS和HPLC-ICP-MS测定浸提液中砷的总量和形态,并研究了10%HNO3对色谱瓶的清洗效果;此外还探讨了温度及时间对玻璃色谱瓶中砷释放的影响.结果表明,玻璃色谱瓶中砷普遍存在,其中硼硅玻璃色谱瓶浸提液中砷浓度低于1μg·L-1,用10%HNO3酸泡24 h能够全部清除;而一级水解级玻璃和高白料玻璃色谱瓶浸提液中砷的浓度则高达45.67μg·L-1和70.25μg·L-1,酸泡24 h后也有较高的残留量,浓度分别为1.63μg·L-1和0.94μg·L-1.高温能促进玻璃色谱瓶中砷的释放;玻璃色谱瓶中砷的释放量随时间的延长而增加,但短时间内即达到平衡.形态分析结果表明,玻璃色谱瓶浸提液中砷形态为As(Ⅴ),没有检测到As(Ⅲ)、二甲基砷酸(DMA)和甲基砷酸(MMA).同时,聚丙烯色谱瓶浸提液中砷总量低于检测限,因而在进行砷形态测定时,建议使用聚丙烯色谱瓶或者经10%HNO3清洗后的硼硅玻璃色谱瓶,以避免玻璃色谱瓶中As(Ⅴ)的污染.     

Inconsistent definitions of "urban" result in different conclusions about the size of urban carbon and nitrogen stocks

There is conflicting evidence about the importance of urban soils and vegetation in regional C budgets that is caused, in part, by inconsistent definitions of "urban" land use. We quantified urban ecosystem contributions to C stocks in the Boston (Massachusetts, USA) Metropolitan Statistical Area (MSA) using several alternative urban definitions. Development altered aboveground and belowground C and N stocks, and the sign and magnitude of these changes varied by land use and development intensity. Aboveground biomass (live trees, dbh > or = 5 cm) for the MSA was 7.2 +/- 0.4 kg C/m2 (mean +/- SE), reflecting a high proportion of forest cover. Vegetation C was highest in forest (11.6 +/- 0.5 kg C/m2), followed by residential (4.6 +/- 0.5 kg C/m2), and then other developed (2.0 +/- 0.4 kg C/m2) land uses. Soil C (0-10 cm depth) followed the same pattern of decreasing C concentration from forest, to residential, to other developed land uses (4.1 +/- 0.1, 4.0 +/- 0.2, and 3.3 +/- 0.2 kg C/m2, respectively). Within a land use type, urban areas (which we defined as > 25% impervious surface area [ISA] within a 1-km(2) moving window) generally contained less vegetation C, but slightly more soil C, than nonurban areas. Soil N concentrations were higher in urban areas than nonurban areas of the same land use type, except for residential areas, which had similarly high soil N concentrations. When we compared our definition of urban to other commonly used urban extents (U.S. Census Bureau, Global Rural-Urban Mapping Project [GRUMP], and the MSA itself), we found that urban soil (1 m depth) and vegetation C stocks spanned a wide range, from 14.4 +/- 0.8 to 54.5 +/- 3.4 Tg C and from 4.2 +/- 0.4 to 27.3 +/- 3.2 Tg C, respectively. Conclusions about the importance of urban soils and vegetation to regional C and N stocks are very sensitive to the definition of urban used by the investigators. Urban areas, regardless of definition, are rapidly expanding in their extent; a systematic understanding of how our development patterns influence ecosystems is necessary to inform future development choices.