Effects of dioxin exposure in Eisenia andrei: integration of biomarker data by an Expert System to rank the development of pollutant-induced stress syndrome in earthworms

A battery of biomarkers has recently been developed in the earthworm Eisenia andrei. In this study, different biomarkers (i.e. Ca²⁺-ATPase activity, lysosomal membrane stability-LMS, lysosomal lipofuscin and neutral lipid content) were utilized to evaluate the alterations in the physiological status of animals, induced by exposure for 3 d to different sublethal concentrations of TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) (1.5 × 10⁻³, 1.5 × 10⁻², 1.5 × 10⁻¹ ng mL⁻¹) utilizing the paper contact toxicity test. Lysosome/cytoplasm volume ratio and DNA damage were also evaluated as a biomarker at the tissue level and as a biomarker of genotoxicity, respectively. Moreover, the NR retention time assay conditions were optimized for the determination of in vivo LMS in earthworm coelomocytes. The results demonstrate that LMS and Ca²⁺-ATPase activity were early warning biomarkers able to detect the effects of minimal amounts of TCDD and that biomarkers evaluated at the tissue level are important for following the evolution of the stress syndrome in earthworms. To evaluate the health status of the animals, an Earthworm Expert System (EES) for biomarker data integration and interpretation was developed. The EES proved to be a suitable tool able to rank, objectively, the different levels of the stress syndrome in E. andrei induced by the different concentrations of TCDD.     

Prenatal detection of monosomy 21 mosaicism

We report a case of chromosomal mosaicism for monosomy 21 revealed in amniotic fluid cell culture. Ultrasound examination at 19 weeks' gestation showed in utero growth retardation and a complex cardiac malformation. A repeated amniocentesis confirmed the presence of monosomy 21 mosaicism. In view of the sonographically detected fetal abnormalities, termination of pregnancy was elected.     

Evaluation of mercury biogeochemical cycling at the sediment–water interface in anthropogenically modified lagoon environments

The Marano and Grado Lagoon is well known for being contaminated by mercury(Hg) from the Idrija mine(Slovenia) and the decommissioned chlor-alkali plant of Torviscosa(Italy).Experimental activities were conducted in a local fish farm to understand Hg cycling at the sediment–water interface. Both diffusive and benthic fluxes were estimated in terms of chemical and physical features. Mercury concentration in sediments(up to 6.81 μg/g)showed a slight variability with depth, whereas the highest methylmercury(MeHg) values(up to 10 ng/g) were detected in the first centimetres. MeHg seems to be produced and stored in the 2–3 cm below the sediment–water interface, where sulphate reducing bacteria activity occurs and hypoxic–anoxic conditions become persistent for days. DMeHg in porewaters varied seasonally(from 0.1 and 17% of dissolved Hg(DHg)) with the highest concentrations in summer. DHg diffusive effluxes higher(up to 444 ng/m~2/day) than those reported in the open lagoon(~ 95 ng/m~2/day), whereas DMeHg showed influxes in the fish farm(up to-156 ng/m~2/day). The diurnal DHg and DMeHg benthic fluxes were found to be higher than the highest summer values previously reported for the natural lagoon environment. Bottom sediments, especially in anoxic conditions, seem to be a significant source of MeHg in the water column where it eventually accumulates. However, net fluxes considering the daily trend of DHg and DMeHg, indicated possible DMeHg degradation processes. Enhancing water dynamics in the fish farm could mitigate environmental conditions suitable for Hg methylation.     

Aerospace wetland monitoring by hyperspectral imaging sensors: a case study in the coastal zone of San Rossore Natural Park

The San Rossore Natural Park, located on the Tuscany (Italy) coast, has been utilized over the last 10 years for many remote sensing campaigns devoted to coastal zone monitoring. A wet area is located in the south-west part of the Natural Park and it is characterized by a system of ponds and dunes formed by sediment deposition occurring at the Arno River estuary. The considerable amount of collected data has permitted us to investigate the evolution of wetland spreading and land coverage as well as to retrieve relevant biogeochemical parameters, e.g. green biomass, from remote sensing images and products. This analysis has proved that the monitoring of coastal wetlands, characterized by shallow waters, moor and dunes, demands dedicated aerospace sensors with high spatial and spectral resolution. The outcomes of the processing of images gathered during several remote sensing campaigns by airborne and spaceborne hyperspectral sensors are presented and discussed. A particular effort has been devoted to sensor response calibration and data validation due to the complex heterogeneity of the observed natural surfaces.     

Traffic-related platinum and rhodium concentrations in the atmosphere of Rome

Platinum and Rh content in the atmosphere of Rome as released by car catalytic converters was monitored from 1998 to 2000 in six urban sites with different traffic intensities and in one rural area. Samples collected with medium-volume PM10 samplers were analyzed by Sector Field Inductively Coupled Plasma Mass Spectrometry (SF-ICP-MS). The Pt content varied from 2.4 to 60.1 pg m(-3) (mean value 17.8 pg m(-3)) at the urban locations whilst that of Rh spanned the range 0.8-9.4 pg m(-3) (average value 4.0 pg m(-3)). The rural area showed metal levels mostly below the limits of detection, pointing to automobile traffic as the main source of those elements in the urban atmosphere. The highest mean concentrations of Pt and Rh, ie. 22.2 and 5.0 pg m(-3), were detected along the ring road where the traffic density is high (>100,000 vehicles per day) and the driving speed between 100 and 120 km h(-1). The lowest Pt and Rh mean concentrations, i.e. 11.4 and 3.4 pg m(-3), were measured downtown, where traffic density is lower (20,000 vehicles per day) and the driving speed is limited (50 km h(-1)). Significant concentrations of Pt and Rh were found in the vicinity of traffic signals, indicating that the "stop-and-go" conditions might also affect their release. The measured Pt/Rh ratio spanned the range 3.3-5.9 in accordance with that present in the more commonly used gasoline car catalytic converters. Seasonal variations between wintertime (with Pt and Rh mean concentrations of 23.8 and 5.1 pg m(-3), respectively) and summertime (with Pt and Rh mean concentrations of 14.1 and 3.3 pg m(-3), respectively) were also observed.     

Role of the second-trimester ‘genetic sonogram’ for Down syndrome screen in the era of first-trimester screening and noninvasive prenatal testing

Ultrasonography for the screening of Down syndrome was first introduced about 25 years ago. Different combinations of markers detectable at second-trimester ultrasonography have been proposed under the banner of ‘genetic sonogram’. In recent years, several developments in first-trimester screening and the recent introduction of noninvasive prenatal testing for aneuploidy screening have had important implications for the prevalence of these conditions in the second-trimester and the screening performance of a genetic sonogram. Several second-trimester sonographic markers for Down syndrome have been reported; meta-analysis has shown that the most powerful predictors are mild ventriculomegaly, increased nuchal fold, hyperechoic bowel, and absent or hypoplastic nasal bone. Whereas use of individual markers should be discouraged and scoring systems of multiple markers are now obsolete, use of combined likelihood ratio and logistic regression analysis formulae provides better accuracy. However, there is significant heterogeneity in results among studies. Despite such limitations, the genetic sonogram will continue to have a place in prenatal screening, particularly in twin and higher-order multiple pregnancies, in countries with limited access to the most recent genetic screening tests, in cases with borderline results at maternal serum screening tests, and as noninvasive supplementary test for high-risk women reluctant to undergo invasive diagnostic testing. © 2014 John Wiley & Sons, Ltd.     

Overview of low molecular weight heparin for preventative treatment of adverse obstetric outcomes related to abnormal placentation

Different proportions of cases of preterm and severe preeclampsia, placental abruption, fetal growth restriction, and fetal death share a common causal pathway of abnormal placental implantation. Documentation of an association between the risk of such adverse pregnancy outcomes (APOs) and inherited thrombophilias prompted initial studies to evaluate the benefit of anticoagulants for the prevention of recurrences both in patients with and without inherited thrombophilias. Prenatal administration of low molecular weight heparin (LMWH) has been evaluated in case control, cohort and randomized clinical trials. The evidence suggests a benefit of LMWH in the reduction of recurrences of APOs, with a number needed to treat of 6 (95% confidence interval: 4–10) to prevent one case of recurrent APOs. Such benefit is independent of the presence of inherited thrombophilias or the administration of low dose aspirin. Further studies are needed to establish the optimal duration for the prophylaxis, to better delineate the mechanism of action of LMWH and to explore the role, if any, of maternal serum markers and uterine artery Doppler findings in the modulation of the LMWH prophylaxis. © 2014 John Wiley & Sons, Ltd.     

Walker-Warburg syndrome: Prenatal ultrasound findings

The prenatal sonographic findings in a case of Walker-Warburg syndrome are described. The patient was not at risk for this condition. Ultrasound examination at 34 weeks' gestation revealed hydrocephaly, Dandy-Walker anomaly, and striking ocular abnormalities. From a review of the literature it appears that while intracranial abnormalities can lead to the diagnosis in cases at risk for this syndrome, ocular abnormalities are rather characteristic for this syndrome and they should be searched for in every case of hydrocephaly or encephalocele.