Persistent Organic Pollutants in gull eggs of two species (Larus michahellis and Larus audouinii) from the Ebro delta Natural Park

The aim of this study was to determine the impact of priority and emerging Persistent Organic Pollutants (POPs) in gull eggs from two species, the scavenger Larus michahellis and the protected species, Larus audouinii. These two species share habitat in the Natural Park of the Ebro delta (Catalonia, Spain). Compounds studied are included or under consideration in the Stockholm Convention and comprise polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs), polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), organochlorinated compounds (OCs), perfluorinated compounds (PFCs) and short chain chlorinated paraffins (SCCPs). Four methods based in selective extraction and gas or liquid chromatography coupled to mass spectrometry were used and quality parameters are provided. OC pesticides and marker PCBs were the most abundant chemical families detected in eggs from the two species, followed by PFCs, PBDEs (especially BDE 209) and SCCPs. Dioxin-like PCBs and PCDD/Fs were also detected in all samples. The overall widespread presence of POPs is discussed in terms of feeding habits, bird ecology and anthropogenic pressures in the protected Ebro delta breeding area.     

Spatial variations of heavy metals contamination in sediments from Odiel river (Southwest Spain)

The presence of heavy metal concentrations was examined in surface sediments from 27 sites within the Odiel river in the southwest Spain suffering inputs from industrial and mining activities. The interest is specially focused on the final delta located in the Odiel Natural Park, which constitutes a feeding ground of international importance for migrating birds. The behavior of 10 heavy metals (Zn, Cd, Pb, Cu, Cr, Mn, Ni, Fe, Hg) is conditioned by chemical properties of sediments such as acidity and carbonate or the presence of sulfate Very acidic sediments in the medium course of river contribute to the lower presence of metal as a consequence of leaching process. However, tidal action raises the pH in the marshes of the delta with correlative metal accumulation. In accordance with the Müller [Umschan 79 (1979) 133-149] scale for geoaccumulation index, the entire watercourse could be considered polluted by Zn and Pb, while Cu exhibits a high presence in the delta marshes of the river.     

Integrated spatiotemporal trends using TRMM 3B42 data for the Upper São Francisco River basin,Brazil

Trend analysis is an important issue for the decision-making processes. Thus, trends of rainfall, consecutive dry days (CDD), and consecutive wet days (CWD) in the Upper São Francisco River basin, Brazil, using daily rainfall data from the Tropical Rainfall Measuring Mission (TRMM) for recent 18 years, were analyzed. Instead of analyzing the trend of one average time series for one specific confidence level, a spatiotemporal analysis over the entire area with 169 continuous time series is done by applying the nonparametric Mann-Kendall and Sen tests for simultaneously 13 confidence levels and a new integrated confidence classification is proposed. The results show that the rainfall has increased during the less rainy periods (from June to October) and has decreased in the rainy periods (from November to May), with the highest and lowest confidence levels, respectively. An analysis of CDD and CWD shows that the number of CDD has decreased, while the number of CWD has increased, which revealed that the dry periods are more frequently interrupted for the period studied.     

<Emphasis Type="Italic">Aporrectodea caliginosa</Emphasis>, a relevant earthworm species for a posteriori pesticide risk assessment: current knowledge and recommendations for culture and experimental design

Ecotoxicological tests with earthworms are widely used and are mandatory for the risk assessment of pesticides prior to registration and commercial use. The current model species for standardized tests is Eisenia fetida or Eisenia andrei. However, these species are absent from agricultural soils and often less sensitive to pesticides than other earthworm species found in mineral soils. To move towards a better assessment of pesticide effects on non-target organisms, there is a need to perform a posteriori tests using relevant species. The endogeic species Aporrectodea caliginosa (Savigny, 1826) is representative of cultivated fields in temperate regions and is suggested as a relevant model test species. After providing information on its taxonomy, biology, and ecology, we reviewed current knowledge concerning its sensitivity towards pesticides. Moreover, we highlighted research gaps and promising perspectives. Finally, advice and recommendations are given for the establishment of laboratory cultures and experiments using this soil-dwelling earthworm species.     

The application of Geographical Information Systems to determine environmental impact significance

This paper presents a new methodology for impact assessment—SIAM (Spatial Impact Assessment Methodology)—which is based on the assumption that the importance of environmental impacts is dependent, among other things, on the spatial distribution of the effects and of the affected environment. The information generated by the use of Geographical Information Systems (GIS) in impact identification and prediction stages of Environmental Impact Assessment (EIA) is used in the assessment of impact significance by the computation of a set of impact indices. For each environmental component (e.g., air pollution, water resources, biological resources), impact indices are calculated based on the spatial distribution of impacts. A case study of impact evaluation of a proposed highway in Central Portugal illustrates the application of the methodology and shows its capabilities to be adapted to the particular characteristics of a given EIA problem.     

Assessing dam implementation impact on threatened carnivores: the case of Alqueva in SE Portugal

Large dam construction in water deficient areas is a management decision often controversial. Besides providing water storage, economical benefits, and a source of renewable energy, the construction and flooding caused by large dams cause disruptions in natural systems. We monitored the pre- and post-Alqueva dam impacts on the threatened carnivore species (polecat, otter, wildcat and Iberian lynx) populations in SE Portugal, and assessed which factors mostly contribute to post-dam distribution. Major short term impacts of large dams are: (1) increase in accessibility and human presence; (2) movement of heavy machinery and dam-workers; (3) deforestation with habitat loss and fragmentation; (4) change from lotic to lentic system; (5) lower prey availability and harsher capture; and (6) changes in land use adjacent to the reservoir. Thus, the response to those impacts can be predicted as a decline of polecat, wildcat and lynx distribution ranges, and a recovery of the otter from the severe short term impacts. Our results corroborate this hypothesis for all the species, especially during deforestation/early flooding. Otter's distribution range increased in the phase of greater impact, with a subsequent decrease with flooding. Our results suggest carnivores used "escape" areas with favourable habitat and prey conditions, however, the areas with higher probability of species presence decreased by two fold showing a drastic range reduction. To ensure populations' survival of these charismatic threatened carnivore populations of Mediterranean landscapes of south-east Portugal, we propose continuing the monitoring program and the development of a conservation program for the subsisting areas of optimal and suboptimal habitats.     

Reactivity of the plant growth regulator paclobutrazol (cultar) with two tropical soils of the northeast semiarid region of Brazil

The reactivity of paclobutrazol (PBZ, a plant growth retardant) with a Yellow Ultisol and a Vertisol from the semiarid northeast region of Brazil was evaluated through batch sorption experiments and modeling. Although not instantaneous, the sorption kinetic of PBZ (pure and formulated) was fast (a few hours) in both soils. The sorption kinetics were well described by a second-order (dS(t)/dt=k(2)(S(e2)-S(t))(2)) but not by a first-order model. The sorption isotherms were found to be linear and the calculated K(D) values were 8.8 +/- 0.11 and 7.4 +/- 0.2 L kg(-1) for pure PBZ in the Ultisol and the Vertisol, respectively. The corresponding K(OC) values were 1275 +/- 34 (logK(OC) = 3.11) and 1156 +/- 49 (logK(OC) = 3.06) L kg(-1), respectively. Considering the very different texture of the two soils and the similar K(OC) values determined, these results showed that in both soils, the sorption of PBZ is dominantly controlled by organic matter, although some interactions of PBZ with iron oxides (goethite) were observed in the Ultisol. Based on these sorption parameters a low leachability potential of PBZ in soils is anticipated, as they correspond to a groundwater ubiquity score (GUS) ranging from 2.0 to 2.7, i.e., moderately to not mobile, in contradiction with the actual groundwater situation in Brazil. This work stresses the need to evaluate and predict the risk associated with aquifer contamination by this widely used plant growth regulator.     

Effectiveness of three configurations of membrane bioreactors on the removal of priority and emergent organic compounds from wastewater: comparison with conventional wastewater treatments

In this work the effectiveness of membrane bioreactors as advanced treatment on the removal of emergent and priority organic compounds in wastewater treatment plants has been evaluated during a one-year monitoring study. The studied wastewater treatment plant operates with flat sheet and hollow fibre membranes in two parallel lines. Moreover, a reverse osmosis module connected in series after the hollow fibre membrane was evaluated for one month. Comparison of membrane bioreactor and conventional activated sludge treatment was also investigated, as well as the influence of the physicochemical properties of the compounds on the removal rates achieved. Sixteen pharmaceutical compounds belonging to seven therapeutic groups and eight priority organic pollutants, including linear alkylbenzene sulfonates, nonylphenol and its ethoxylates and phthalate, were monitored. The highest mean concentrations corresponded to priority organic pollutants (309 μg L(-1) of linear alkylbenzene sulfonate C(12)) followed by pharmaceutical compounds (24.5 μg L(-1) of ibuprofen). No significant difference of effectiveness was found among flat sheet and hollow fibre membranes. However, an improvement was obtained with the addition of a reverse osmosis module for most of the compounds. Biodegradation has been shown as the main route involved in the removal of organic compounds during both technologies.     

Evaluation of optimal conditions for determination of low selenium content in shellfish samples collected at Todos os Santos Bay,Bahia, Brazil using HG-AFS

This work proposes a procedure for the determination of total selenium content in shellfish after digestion of samples in block using cold finger system and detection using atomic fluorescent spectrometry coupled hydride generation (HG AFS). The optimal conditions for HG such as effect and volume of prereduction KBr 10 % (m/v) (1.0 and 2.0 ml) and concentration of hydrochloric acid (3.0 and 6.0 mol L^?1) were evaluated. The best results were obtained using 3 mL of HCl (6 mol L^?1) and 1 mL of KBr 10 % (m/v), followed by 30 min of prereduction for the volume of 1 mL of the digested sample. The precision and accuracy were assessed by the analysis of the Certified Reference Material NIST 1566b. Under the optimized conditions, the detection and quantification limits were 6.06 and 21.21 μg kg^?1, respectively. The developed method was applied to samples of shellfish (oysters, clams, and mussels) collected at Todos os Santos Bay, Bahia, Brazil. Selenium concentrations ranged from 0.23?±?0.02 to 3.70?±?0.27 mg kg^?1 for Mytella guyanensis and Anomalocardia brasiliana, respectively. The developed method proved to be accurate, precise, cheap, fast, and could be used for monitoring Se in shellfish samples.     

Natural regeneration of the herbaceous community in a semiarid region in Northeastern Brazil

This study aimed to describe and compare the interannual changes in the diversity and population structure of herbaceous plants in an anthropogenic area that has been regenerating for 15 years and to identify the similarities and differences in the biological attributes of the community compared with the characteristics of a regenerating conserved area. In total, 105 plots measuring 1 m² were established. In each plot, the herbaceous plants were identified, and their height and stem diameter were measured for two consecutive years. The herbaceous flora of the anthropogenic area was represented by 86 species in 70 genera and 27 families, and there were no significant differences in the average richness between years. The conserved area was represented by 71 species in 63 genera and 35 families, and there was a significant difference in the total richness between areas and between years, except when comparing the richness between the conserved area and the anthropogenic area during the second year. Considering both the anthropogenic and conserved areas, 123 herbaceous species were listed, and the similarity between areas was 60 %. For the anthropogenic area, the floristic similarity between years was 95 %, and in the fragment of the conserved area, the similarity was 74 %. The diversity and density were significantly different between years and between areas. Given these results, this study suggests that 15 years of natural regeneration for the caatinga is not sufficient to reestablish its native flora with respect to its herbaceous component.