Applying habitat and population-density models to land-cover time series to inform IUCN Red List assessments

The IUCN (International Union for Conservation of Nature) Red List categories and criteria are the most widely used framework for assessing the relative extinction risk of species. The criteria are based on quantitative thresholds relating to the size, trends, and structure of species’ distributions and populations. However, data on these parameters are sparse and uncertain for many species and unavailable for others, potentially leading to their misclassification or classification as data deficient. We devised an approach that combines data on land-cover change, species-specific habitat preferences, population abundance, and dispersal distance to estimate key parameters (extent of occurrence, maximum area of occupancy, population size and trend, and degree of fragmentation) and hence predict IUCN Red List categories for species. We applied our approach to nonpelagic birds and terrestrial mammals globally (∼15,000 species). The predicted categories were fairly consistent with published IUCN Red List assessments, but more optimistic overall. We predicted 4.2% of species (467 birds and 143 mammals) to be more threatened than currently assessed and 20.2% of data deficient species (10 birds and 114 mammals) to be at risk of extinction. Incorporating the habitat fragmentation subcriterion reduced these predictions 1.5–2.3% and 6.4–14.9% (depending on the quantitative definition of fragmentation) for threatened and data deficient species, respectively, highlighting the need for improved guidance for IUCN Red List assessors on the application of this aspect of the IUCN Red List criteria. Our approach complements traditional methods of estimating parameters for IUCN Red List assessments. Furthermore, it readily provides an early-warning system to identify species potentially warranting changes in their extinction-risk category based on periodic updates of land-cover information. Given our method relies on optimistic assumptions about species distribution and abundance, all species predicted to be more at risk than currently evaluated should be prioritized for reassessment.     

Fetoplacental discrepancy involving structural abnormalities of chromosome 8 detected by prenatal diagnosis

We describe the finding of three cell lines involving different structural abnormalities of chromosome 8 detected in a prenatal diagnosis. Chorionic villi sampling (CVS) was performed on a pregnant woman because of advanced maternal age. Semidirect cytogenetic analysis showed a mos46,XX,i(8q)/46,XX,del(8)(p11.2) karyotype, confirmed by fluorescence in situ hybridization (FISH). Amniocentesis was subsequently performed, and the karyotype obtained was 46,XX,dup(8)(p23p11.2). The pregnancy was terminated; pathologic findings included clubfeet, clenched left hand, subcutaneous edema and bilateral hydrocephalus. Molecular studies using chromosome 8 microsatellites performed on parents' blood and fetal tissues revealed a maternal meiotic origin of the inv dup(8p) with deletion of the distal p23 region and duplication of the remaining 8p. We propose a model to explain the cytogenetic findings, which includes a first maternal meiotic error giving rise to a large dicentric isochromosome 8 present in the ovum, a second error in one of the first zygote divisions with misdivision of the dicentric 8 giving rise to a cell line with del(8p) confined to the trophoblast and another cell line with inv dup(8p) confined to the fetal tissue and a third error in the trophoblast giving rise to a further cell line with isochromosome 8q. Copyright © 2003 John Wiley & Sons, Ltd.     

Ozonisation coupled with biological degradation for treatment of phenolic pollutants: a mechanistically based study

Phenolic acids constitute an important group of pollutants which are reluctant to biological treatment. Solutions containing a mixture of cinnamic acid, p-coumaric acid, caffeic acid and ferulic acid were submitted to ozonisation. Then, the changes in biodegradability along the process were studied by means of respirometry. There is an optimum ozone dosage in the interval 3-5 min of treatment which allows to achieve the maximum increase in biodegradability (more than 10 times) and a high efficiency of the ozonisation process (COD decreases to a half of its initial value). Further ozonisation does not help to increase biodegradability and is clearly disadvantageous. Similar results are obtained with actual samples of olive oil wastewaters. This behaviour is explained based on the formation of highly biodegradable benzaldehydes as key ozonisation intermediates, in the early reaction stages.     

Possible influence of lipid content on levels of organochlorine compounds in mussels from Galicia coast (Northwestern, Spain). Spatial and temporal distribution patterns

Influence of lipid content of mussel on levels of organochlorine compounds (polychlorinated biphenyls [PCBs] and OC pesticides) has been studied. Mussel samples coming from three sites in Galicia coast (Northwestern, Spain), Moa?a and Cangas C from Ría de Vigo and Barallobre from Ría de Ferrol, collected monthly in the period from November 2000 to October 2001, have been analysed. Organochlorine compounds, PCBs (IUPAC nos. 31, 28, 52, 101, 118, 153, 105, 138, 156 and 180) and OC pesticides (pp'-DDE, op'-DDT, pp'-DDT, aldrin, isodrin and endrin) have been extracted by means of Soxhlet and determined by using gas chromatography (electron capture detector [ECD]) and gas chromatography-mass spectrometry. Statistical analysis has been performed using the SPSS statistical package. Few compounds were correlated with lipid content (Pearson product-moment correlation); for samples coming from Barallobre, only the individual PCBs 31, 28, 52 (tri- and tetrachlorinated biphenyls) and 180 (heptachlorinated biphenyl) presented a significant correlation; for Moa?a samples, two cyclodiene pesticides, endrin and isodrin, presented a slight significant correlation with lipid content. From these results, it is deduced that the normalization of the concentration of organochlorine compounds to the lipid content is not necessary, in this way, avoiding possible error sources associated to the analytical determination of the lipid contentor reproductive status of mussel. Multivariate techniques (PCA) have been employed in order to study spatial and temporal distribution patterns of organochlorine compounds. The great differences found in studied samples are mainly related to spatial distribution: separations between estuarine bays (samples coming from Ría de Vigo and Ría de Ferrol) and between rural and urban zones (samples coming from Moa?a and Cangas C and samples from Barallobre, respectively).     

Glutathione S-tranferases and cytochrome P450 activities in Mytilus galloprovincialis from the South coast of Portugal: effect of abiotic factors

To assess the health of aquatic organisms, biomarkers that measure changes at the biochemical level have been used as effective early warning tools in ecological risk assessment. In order to develop an integrated risk assessment strategy for the south coast of Portugal, mussels Mytilus galloprovincialis were collected from six sites along the coast with different organic contaminant characteristics. Additionally, an active biomonitoring approach was followed by transplanting indigenous mussels from site 4 to 6 and vice versa (from site 6 to 4) for 28 days. PAHs and PCBs contents were measured and the associated responses of phase I and phase II detoxification mechanisms evaluated by measuring cytochrome P450 and GST activities. GST activity was also determined on different tissues (gills, digestive gland, foot, mantle and gonads) of M. galloprovincialis and the impact of abiotic parameters (temperature, salinity, pH, conductivity and dissolved oxygen) on the GST activity assessed. Results indicate that CYP 450 follow the same pattern of PAHs and a direct relationship exist between CYP 450 and PAH levels from the different sites. Although there is a decrease between GST and PAHs concentrations it was not significant. The majority of the GST activity was in the gills and the digestive gland (around of 75% of the activity measured in all tissues) followed in decreasing order by the mantle, gonads. An inverse relationship between GST activity and salinity was detected along with temperature although not significant. These two biomarkers respond to changes of these two groups of compounds and to salinity especially for GST. In conclusion CYP 450 in mussels gives a reliable response as biomarker for organic contaminants in risk assessment in the South Coast of Portugal.     

Concentrations of PCDD/PCDFs and PCBs in fish and seafood from the Catalan (Spain) market: estimated human intake

The concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs) and biphenyls (PCBs) in samples from 14 fish and seafood species widely consumed by the population of Catalonia, Spain, were measured. These samples were randomly purchased independently of their geographical origin. The intake of PCDD/Fs and PCBs through consumption of these species was also estimated for various age and sex groups of this population. The highest and lowest levels of PCDD/Fs and dioxin-like PCBs (DL-PCBs) were found in red mullet and shrimp, respectively. For a standard adult man (70 kg body weight), the intake of PCDD/Fs plus DL-PCBs through consumption of fish and other seafood was estimated to be 38.0 pg WHO-TEQ/day. Tuna, hake, and sardine were the species with the highest contribution to this intake. The results of this study indicate that, in general terms, the dietary habits of the population of Catalonia (Spain) regarding fish and seafood consumption do not contribute remarkably to increase PCDD/F and DL-PCB intake.     

A Regional Modeling Framework of Phosphorus Sources and Transport in Streams of the Southeastern United States1

García, Ana María, Anne B. Hoos, and Silvia Terziotti, 2011. A Regional Modeling Framework of Phosphorus Sources and Transport in Streams of the Southeastern United States. Journal of the American Water Resources Association (JAWRA) 47(5):991‐1010. DOI: 10.1111/j.1752‐1688.2010.00517.x Abstract: We applied the SPARROW model to estimate phosphorus transport from catchments to stream reaches and subsequent delivery to major receiving water bodies in the Southeastern United States (U.S.). We show that six source variables and five land‐to‐water transport variables are significant (p < 0.05) in explaining 67% of the variability in long‐term log‐transformed mean annual phosphorus yields. Three land‐to‐water variables are a subset of landscape characteristics that have been used as transport factors in phosphorus indices developed by state agencies and are identified through experimental research as influencing land‐to‐water phosphorus transport at field and plot scales. Two land‐to‐water variables – soil organic matter and soil pH – are associated with phosphorus sorption, a significant finding given that most state‐developed phosphorus indices do not explicitly contain variables for sorption processes. Our findings for Southeastern U.S. streams emphasize the importance of accounting for phosphorus present in the soil profile to predict attainable instream water quality. Regional estimates of phosphorus associated with soil‐parent rock were highly significant in explaining instream phosphorus yield variability. Model predictions associate 31% of phosphorus delivered to receiving water bodies to geology and the highest total phosphorus yields in the Southeast were catchments with already high background levels that have been impacted by human activity.     

Maternal energy expenditure does not change with flight costs or food availability in the pied flycatcher (Ficedula hypoleuca): costs and benefits for nestlings

We manipulated parental work load without changing brood size in a population of pied flycatchers Ficedula hypoleuca by removing two primaries (7 and 9) from each wing of females, thus reducing wing area and increasing flight costs. At other nests, we offered supplementary food in the form of live mealworms (10–20 g daily from hatching) to reduce brood demand and thus parental foraging costs. Other nests were left as controls. The daily energy expenditure of females feeding 12-day-old nestlings was measured with doubly labelled water D₂ ¹⁸O. Females in both treatments expended the same amount of energy, fed at the same rate and had similar body masses to birds in the control group. No effect of treatment on male mass and feeding effort was detected. More nestlings, however, died in nests of handicapped females. Nestlings of handicapped females had significantly lower body mass and haematocrit values than nestlings in food-supplemented nests, with nestlings in control nests occupying an intermediate position. The effects of both treatments on nestling mass, haematocrit values and mortality rates were only noticeable in nests infested with mites. Maternal energy expenditure is apparently constrained and offspring pay the costs imposed by reduced provisioning rate or increased demand caused by ectoparasites, while receiving benefits when food supply improves. The presumption that avian reproductive costs derive from changes in a flexible energy output may not be met in many cases. Received: 24 October 1998 / Received in revised form: 15 March 1999 / Accepted: 26 April 1999