Phytomanagement of Cd-contaminated soils using maize (Zea mays L.) assisted by plant growth-promoting rhizobacteria

Zea mays (L.) is a crop widely cultivated throughout the world and can be considered suitable for phytomanagement due to its metal resistance and energetic value. In this study, the effect of two plant growth-promoting rhizobacteria, Ralstonia eutropha and Chryseobacterium humi, on growth and metal uptake of Z. mays plants in soils contaminated with up to 30 mg Cd kg^?1 was evaluated. Bacterial inoculation increased plant biomass up to 63 % and led to a decrease of up to 81 % in Cd shoot levels (4–88 mg Cd kg^?1) and to an increase of up to 186 % in accumulation in the roots (52–134 mg Cd kg^?1). The rhizosphere community structure changed throughout the experiment and varied with different levels of Cd soil contamination, as revealed by molecular biology techniques. Z. mays plants inoculated with either of the tested strains may have potential application in a strategy of soil remediation, in particular short-term phytostabilization, coupled with biomass production for energy purposes.     

Practices and opportunities of ecosystem service studies for ecological restoration in China

New approaches have emerged in integrating ecosystem services, their structure and function for ecological restoration. As the concept relating with ecosystem services has become widely accepted in last decade in China, its linking with ecological restoration will provide good opportunities and make substantial progress towards sustainable development. By analyzing the traditional ecological restoration procedure in China, we developed a framework of ecological restoration based on ecosystem services valuation. Relevant researches of ecological restoration based on ecosystem services are also carefully reviewed. China’s ecosystem services studies and payments for ecosystem services (PES) experiences were extensively discussed to show the ecological restoration strategy and its change. Two case studies in China were presented to show the framework for ecological restoration based on PES. We suggested that despite the limitations of valuation uncertainties at present, PES could be an opportunity for ecological restoration by evaluating the trade-offs of different stakeholders. In current state in China, challenges and opportunities coexist and scientists should work together in the integrated research of sustainable ecological management and economic development policies.     

Nitrogen oxides and ozone in Portugal: trends and ozone estimation in an urban and a rural site

Environmental Science and Pollution Research - This study provides an analysis of the spatial distribution and trends of NO, NO2 and O3 concentrations in Portugal between 1995 and 2010....     

Social acceptance of wind and solar power in the Brazilian electricity system

Renewable energy is often associated with the production of clean electricity and free of significant adverse impacts. However, several studies have been highlighting the importance of the assessment of social impacts of these technologies, including not only the benefits but also the potential negative aspects most frequently affecting local population. The energy matrix in Brazil is already built up on a renewable system largely supported on hydropower but other technologies with special emphasis on wind power start to have a major role, with a strong growth forecasted for this sector in the coming years. This article discusses the integration of solar and wind power in the Brazilian electricity system focusing on the social awareness and acceptance for the population living in high potential regions. For this, a questionnaire was proposed with the aim of evaluating the level of knowledge of wind and solar power, their social acceptance and perceptions towards cost, local development and environmental impacts. The questionnaire was implemented in an academic institution in the State of Rio Grande do Norte (RN) including students and professors as key actors for the present and future energy policy decision making. The implementation process and the obtained results are described allowing to conclude on the high level of acceptance of solar and wind power in the country and the region, with low evidence of not in my backyard syndrome.     

Influence of selected anti-cancer drugs on the induction of DNA double-strand breaks and changes in gene expression in human hepatoma HepG2 cells

In chemotherapy, various anti-cancer drugs with different mechanisms of action are used and may represent different risk of undesirable delayed side effects in treated patients as well as in occupationally exposed populations. The aim of the present study was to evaluate genotoxic potential of four widely used anti-cancer drugs with different mechanisms of action: 5-fluorouracil (5-FU), cisplatin (CDDP) and etoposide (ET) that cause cell death by targeting DNA function and imatinib mesylate (IM) that inhibits targeted protein kinases in cancer cells in an experimental model with human hepatoma HepG2 cells. After 24 h of exposure all four anti-cancer drugs at non-cytotoxic concentrations induced significant increase in formation of DNA double strand breaks (DSBs), with IM being the least effective. The analysis of the changes in the expression of genes involved in the response to DNA damage (CDKN1A, GADD45A, MDM2), apoptosis (BAX, BCL2) and oncogenesis (MYC, JUN) showed that 5-FU, CDDP and ET upregulated the genes involved in DNA damage response, while the anti-apoptotic gene BCL2 and oncogene MYC were downregulated. On the contrary, IM did not change the mRNA level of the studied genes, showing different mechanism of action that probably does not involve direct interaction with DNA processing. Genotoxic effects of the tested anti-cancer drugs were observed at their therapeutic concentrations that may consequently lead to increased risk for development of delayed adverse effects in patients. In addition, considering the genotoxic mechanism of action of 5-FU, CDDP and ET an increased risk can also not be excluded in occupationally exposed populations. The results also indicate that exposure to 5-FU, CDDP and ET represent a higher risk for delayed effects such as cancer, reproductive effects and heritable disease than exposure to IM.     

Indirect land use change from ethanol production: the case of sugarcane expansion at the farm level on the Brazilian Cerrado

The expansion of sugarcane production in the Brazilian Cerrado has resulted in indirect land use change (ILUC), occurring when displaced land uses in one location are reallocated to another. Studies, however, usually identify ILUC at the regional or national level far from the original area of a displaced land use. This study examines the occurrence of ILUC due to sugarcane expansion for ethanol production at the farm level in the Brazilian Cerrado. It fills a gap in the literature by examining socioeconomic, policy and farm-level factors that influence ILUC at the farm level in the Brazilian Cerrado using face-to-face enumerated surveys. Results indicate that ILUC did occur at the farm (producer) scale and farmers who undertook ILUC intensified agricultural production on their farms. Results inform policymakers on how the intensification of agricultural practices may make it potentially difficult to keep protected lands out of production, reducing the environmental benefits from sugarcane-based biofuel production.     

Valorization of fish by-products: rheological,textural and microstructural properties of mackerel skin gelatins

The fish processing industry generates significant amounts of waste which is usually discarded. The present study investigated the recovery of gelatins from Atlantic mackerel (Scomber scombrus) skins after pre-treatment with different environmentally friendly organic acids (acetic, citric, lactic, tartaric or malic acid). The chemical composition, the rheological and the textural properties as well as the microstructural characteristics of the extracted gelatins were analysed and compared to commercial bovine hide gelatin. Although the organic acid used in the pre-treatment step did not affect the extraction yield and the chemical composition of the prepared gelatins, differences were observed in terms of rheology and texture. The highest gel strength (P < 0.05) was observed with gelatins extracted after pre-treatment with acetic, citric and malic acids (71–80 g). From an industrial point of view, gelatin can be extracted using any of these organic acids with similar yield. However, in order to obtain better rheological and textural properties the use of acetic, citric or malic acid in the pre-treatment step is recommended.     

Characterization and application of the enzyme peroxidase to the degradation of the mycotoxin DON

Deoxynivalenol (DON), one of the main mycotoxins found in food matrices, has high level of toxicity. This study aimed to characterize the peroxidase enzyme extracted from rice bran to be applied to the biodegradation of DON in order to evaluate the potential peroxidase (PO) from rice bran (RB) has to degrade DON in optimal conditions. Purification and recovery factors of PO extracted from RB and purified by three-phase partitioning were 5.7% and 50%, respectively. PO had the highest level of activity in the phosphate buffer 5 mM pH 5.5 in both crude and purified forms, whose reaction temperatures were 25°C and 10°C. At the end of production, purification and characterization steps, specific activities of the bran were 115.79 U mg^?1 and 4363 U g^?1. Reduction in the mycotoxin DON in optimal conditions determined for PO from RB was 20.3%, a promising result when the aim is to adequate mycotoxicological levels to foods.     

Aminocyclopyrachlor sorption-desorption and leaching from three Brazilian soils

This study aimed to evaluate the sorption-desorption and leaching of aminocyclopyrachlor from three Brazilian soils. The sorption-desorption of ¹⁴C-aminocyclopyrachlor was evaluated using the batch method and leaching was assessed in glass columns. The Freundlich model showed an adequate fit for the sorption-desorption of aminocyclopyrachlor. The Freundlich sorption coefficient [K_{f (sorption)}] ranged from 0.37 to 1.34 µmol ^(1–1/n) L^1/n kg^?1 and showed a significant positive correlation with the clay content of the soil, while the K_{f (desorption)} ranged from 3.62 to 5.36 µmol ^(1–1/n) L^1/n kg^?1. The K_{f (desorption)} values were higher than their respective K_{f (sorption)}, indicating that aminocyclopyrachlor sorption is reversible, and the fate of this herbicide in the environment can be affected by leaching. Aminocyclopyrachlor was detected at all depths (0?30 cm) in all the studied soils, where leaching was influenced by soil texture. The total herbicide leaching from the sandy clay and clay soils was <0.06%, whereas, ～3% leached from the loamy sand soil. The results suggest that aminocyclopyrachlor has a high potential of leaching, based on its low sorption and high desorption capacities. Therefore, this herbicide can easily contaminate underground water resources.     

Occurrence and behaviour of pharmaceutical compounds in a Portuguese wastewater treatment plant: Removal efficiency through conventional treatment processes

Wastewater treatments can eliminate or remove a substantial amount of pharmaceutical active compounds (PhACs), but there may still be significant concentrations of them in effluents discharged into surface water bodies. Beirolas wastewater treatment plant (WWTP) is located in the Lisbon area and makes its effluent discharges into Tagus estuary (Portugal). The main objective of this study is to quantify a group of 32 PhACs in the different treatments used in this WWTP. Twelve sampling campaigns of wastewater belonging to the different treatments were made in 2013–2014 in order to study their removal efficiency. The wastewaters were analysed by solid phase extraction (SPE) and ultra-performance liquid chromatography coupled with tandem mass detection (UPLC–MS/MS). The anti-diabetics were the most frequently found in wastewater influent (WWI) and wastewater effluent (WWE) (208 and 1.7 μg/L, respectively), followed by analgesics/antipyretics (135 μg/L and < LOQ, respectively), psychostimulants (113 and 0.49 μg/L, respectively), non-steroidal anti-inflammatory drugs (33 and 2.6 μg/L, respectively), antibiotics (5.2 and 1.8 μg/L, respectively), antilipidemics (1.6 and 0.24 μg/L, respectively), anticonvulsants (1.5 and 0.63 μg/L, respectively) and beta blockers (1.3 and 0.51 μg/L, respectively). A snapshot of the ability of each treatment step to remove these target PhACs is provided, and it was found that global efficiency is strongly dependent on the efficiency of secondary treatment. Seasonal occurrence and removal efficiency was also monitored, and they did not show a significant seasonal trend.