A fetus with a parvovirus B19 infection and congenital anomalies

A fetus with multiple structural defects was seen at prenatal ultrasound examination. After termination of the pregnancy a bilateral cleft lip, alveolus, and palate; micrognathia; and webbed joints were seen. Fetal tissues showed indications of infection, intranuclear inclusion bodies, chronic stress, haemolysis, arterial wall damage, and profuse haemorrhage. Parvovirus B19 DNA was detected in fetal tissues by dot hybridization after polymerase chain reaction. The possibility of parvovirus B19 infection leading to congenital malformations is discussed.     

Upgrading of organic waste: production of the copolymer poly-3-hydroxybutyrate-co-valerate by Ralstonia eutrophus with organic waste as sole carbon source

Two types of fermented organic waste (trade and industry waste and fruit and vegetable waste) were successfully used as a sole carbon source to produce poly-3-hydroxybutyrate-co-valerate (PHBV) by Ralstonia eutrophus (formerly Alcaligenes eutrophus) via oxygen limitation.The production of PHBV could be optimized by optimizing the oxygen transfer through the fermentor. Thereby, a peak concentration of 1.1 g PHBV per liter cell suspension, 40 w% of cell dry weight, was obtained at an aeration rate of 0.24 mol O₂/h·kg biomass. The yield of PHBV on the fatty acid concentration in the organic waste was 0.16 g polymer/g volatile organic matter. The process obtained, compares well with the commercial production process of PHBV based on glucose.     

Prenatal diagnosis in two cases of de novo complex balanced chromosomal rearrangements. Three-year follow-up in one case

We report two cases of apparently balanced complex de novo chromosomal rearrangements (BCCR) detected prenatally at 17 weeks and 10 weeks of gestation, respectively. Chromosomes were studied using GTG-banding and fluorescent in situ hybridization (FISH). In one case four chromosomes and in the other case three chromosomes were involved in the rearrangements. One of the pregnancies was terminated and no external or internal abnormalities were detected at autopsy. The other pregnancy continued to term. Level III ultrasound examination showed no abnormalities. The child is now 3 years old and has neither congenital anomalies nor evidence of delayed psychomotor development.     

Human parvovirus B19 infection and unbalanced translocation in a case of hydrops fetalis

In a case of hydrops fetalis, serological examination showed a recent maternal human parvovirus B19 infection. Amniocentesis revealed a unique unbalanced translocation between chromosomes 3 and 11 of the fetus. The mother proved to have a balanced reciprocal translocation between chromosomes 3 and 11. A grossly macerated hydropic male fetus was delivered with a flat nose and low implanted deformed ears. Histopathological examination revealed nuclear inclusion bodies in fetal erythroid cells, confirming human parvovirus B19 infection. Parvovirus B19 DNA was demonstrated by in situ hybridization in the nuclei of heart muscle cells. Our finding of two different disorders in one case illustrates the importance of a complete evaluation of every case of hydrops fetalis, especially concerning counselling on the outcome of future pregnancies. The human parvovirus B19 infection will not recur due to the acquired immunity of the mother, whereas the balanced reciprocal translocation will endanger future pregnancies.     

Opportunities and constraints for Product-oriented Environmental Management Systems (P-EMS)

Product-oriented Environmental Management Systems (P-EMS) are a logical extension of current, often primarily process- of facility-oriented Environmental Management Systems. The Dutch government promotes P-EMS as part of its product-oriented environmental policies. The idea of P-EMS is to enable producers to make environmentally responsible decisions on product development and product strategies, in collaboration with suppliers and customers. This paper summarises example P-EMS projects in The Netherlands for two segments of industry, namely retail and manufacturing industry. Moreover two other initiatives will be covered that deal with the development of tools to facilitate P-EMS in two other industry sectors (food processing and building). Success factors that emerge from these initial experiences are: focus on partners that have a strong incentive to act, application of screening Life Cycle Assessments (LCA) or user-shells for LCA, and focus on improvements that can be implemented on the short term.     

欧洲的氮状况

欧洲(不包括前苏联)的氮收支表明,欧洲氮循环加速的三个主要驱动力是肥料生产(14MtN/a)、矿物燃料燃烧及其它工业(3.3MtN/a)和各种产品中的氮输入(7.6MtN/a).本文估计了欧洲粮食、能源和工业产品系统中活性氮元素的各种泄漏,评估了它们对人类健康及水陆生态系统的影响.考虑到氮流转中的可能后果,未来欧洲有关封闭氮循环和减少活性氮泄漏的环境政策措施最好集中于三个主要驱动力.在确定氮排放上限和制定氯流控制的综合政策方面,如肥料使用、输入和氮水平,临界负载可能是非常有用的工具.