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511.
The mouthparts of Munida sarsi Huus, 1935, including the labrum and paragnaths, are described using LM and SEM, with special attention to the setal composition
and its function. We also used high-resolution macrovideo equipment to record the feeding processes and monitor setae in action.
This combination of morphological and preliminary behavioral data enabled us to arrive at a more comprehensive understanding
of the functional morphology. M. sarsi was found to be an opportunist and capable of handling and eating most kinds of animal tissue. The species can also be a
selective deposit feeder, sorting the sediment by quality and not by size. This sorting is performed by the setae on the medial
rim of the bases of maxilla 1, maxilla 2 and maxilliped 1. Many of the other mouthpart functions ranging from food detection
to ingestion are mapped with comparison to what has been found for other anomurans. Grooming was shown to be a significant
function of many mouthparts, especially the endopod of maxilliped 3. In general the mouthparts closest to the mouth have the
least complex movements and functional scheme, with the mandibles being able to perform only one movement, i.e. cutting or
crunching food objects. Some functions are attributed to the paragnath, the endopod of maxilla 2 and the epipod of maxilliped
1.
Received: 3 October 1999 / Accepted: 22 March 2000 相似文献
512.
Genetic variation in 15 Holothuria (Microthele) nobilis (Selenka, 1867) populations on the Great Barrier Reef was studied at seven polymorphic allozyme loci. Although populations
were separated by distances up to 1300 km, there were no apparent restrictions to gene flow (F
ST
values were not significantly different from 0) and the maximum Nei's unbiased genetic distance was 0.003. Populations were
in Hardy–Weinberg equilibrium at all loci. The estimated maximum sexual input [this is the ratio of the number of sexually
produced individuals (N*) to the sample size (N
i
)] and the minimum sexual input [this is the ratio of the number of genotypes (N
go
) over the sample size (N
i
)] were used as estimators for the amount of asexual reproduction. Both parameters suggested that H. nobilis reproduces solely by sexual means (N*:N
i
: = 1; N
go
:N
i
= 0.74 to 1). The allozyme data indicated high gene flow between populations, but the possibility that allozyme frequencies
may not be at equilibrium means that it was not possible to distinguish whether the patterns reflect present-day dispersal
or dispersal that occurred in the past.
Received: 27 March 2000 / Accepted: 29 June 2000 相似文献
513.
The nature of heat coma was examined in the edible periwinkle Littorina littorea (L.). Duration of acclimation did not influence heat-coma temperature at 12 °C, although other acclimation temperatures were
important in influencing thermal tolerance, with positive shifts in coma temperature observed in response to elevated temperatures.
Previous thermal history also influenced heat-coma temperatures. Individuals subjected to repeat heat-coma events on a daily
basis showed significant declines (P < 0.05) in coma-temperature; in contrast individuals exposed to repeat heat-coma events on a weekly basis showed no decline
in thermal tolerance. Size-effects occurred at selected sites, where decreased heat-coma temperatures were recorded in large
individuals.
Received: 16 August 1999 / Accepted: 13 June 2000 相似文献
514.
The synthesis and secretion of the humoral opsonin from the colonial ascidian Botryllus schlosseri was studied by using specific polyclonal antibodies that recognise both of the bands obtained after SDS-PAGE of the purified
lectin. The agglutinin was revealed on the surface of agglutinated yeast and red blood cells and in the cytoplasm of the phagocytes,
either uniformly distributed or localised inside the cytoplasmic projections. A previous incubation of haemocytes with the
agglutinin resulted in intense labelling of the surface that disappeared in the presence of D-galactose, whereas it was not affected by treatment with proteases. These results, together with the already published data,
suggest that our molecule is a new member of the galectin family.
Received: 16 July 1999 / Accepted: 7 February 2000 相似文献
515.
Spatial and temporal patterns of predation on seeds of the seagrass Phyllospadix torreyi S. Watson were quantified at four sites near Santa Barbara, California, USA. Over a period of four flowering seasons during
1995 to 1998, monthly patterns of seed fall and intensity of seed predation were similar among sites, but were temporally
quite variable. Abundance of dispersed seeds varied greatly both among seasons and years. Within any one year, seeds were
present in the environment every month, but they peaked in abundance during the fall months following the annual flowering
period. Seeds were more abundant during the earlier years of the study. The intensity of predation steadily increased throughout
the study period, from a low of ≤10% seeds consumed during 1995 to ∼50% consumption by 1997, and it was not correlated with
abundance of seeds in the environment. Pre-dispersal seed loss also was estimated in two flowering seasons by counting the
numbers of seeds consumed prior to release from the plant, and was relatively low (<15%). No differences were detected among
the four study sites in patterns of pre-dispersal loss. Field surveys were done at two sites to identify potential seed predators.
Three of the most abundant species identified in these surveys were tested in the laboratory to determine size-specific patterns
of feeding activity and to assess which early life stages of P. torreyi (fruits within spadices, dehisced fruits, seedlings) were most vulnerable to predation. All three of the early life stages
were consumed. The crabs Pugettiaproducta (Randall) and Pachygrapsus crassipes Randall were much more voracious predators than the isopod Idotea resecata Stimpson.
Received: 8 June 1999 / Accepted: 2 February 2000 相似文献
516.
Early development of the Antarctic sea urchin Sterechinus neumayeri was examined under two differ-ent culture regimes: one to simulate development near-bottom (“demersal development”) and the
other to simulate the development of embryos in the water column (“pelagic development”). When embryos of both treatments
reached the hatching blastula stage at 5 d post-fertilization (−1.5 °C), the blastulae that had undergone demersal development
evidenced significant differences (by ANOVA or suitable non-parametric comparison) in the following: a thicker blastoderm
layer (12%, P < 0.001), higher ash-free dry weights (19%, P < 0.01), lower mass-specific respiration rates (50%, P < 0.001), higher incorporation rates of 35S-methionine into protein (23%, P < 0.003), and a differential pattern of protein synthesis. When embryos developed demersally, they remained in the jelly-coat
material released with the eggs at spawning. Quantitative isolation of this jelly-coat material in S. neumayeri demonstrated that it contained a significant amount of organic matter, 115 ng ash-free dry mass per egg, equivalent to 17%
of the egg's initial organic mass. Uptake of external nutrients during embryogenesis may be a significant component of the
physiological energetics of this polar invertebrate by allowing the utilization of jelly-coat material released by a female
during spawning.
Received: 21 April 1999 / Accepted: 5 June 2000 相似文献
517.
The relationship between sperm characteristics and reproductive success was examined in male herring, Clupea harengus L. Males were categorised as being first-time or repeat spawners on the basis of their age; they were also grouped according
to whether their sperm were immediately active and exhibited forward motion on contact with seawater (FM) or had little or
only vibratory motion (VM). Unlike the Pacific herring C. pallasii Valencienes, Atlantic herring sperm is usually motile on contact with seawater. The age, weight and gonadosomatic index (testes
mass as a percentage of somatic mass = GSI) were measured and used as characteristics for individual fish. Sperm traits measured
were (1) adenosine triphosphate (ATP) concentration, (2) sperm count, (3) duration of sperm motility. Reproductive success
for each male was estimated from the fertilisation rate and from the length of larvae at hatching. Fertilisation rates for
all fish were generally >80%. The ATP concentration of non-activated spermatozoa was negatively correlated with fertilisation
rate. Among repeat spawners, fish with higher GSIs produced larvae that were larger at hatching. Although VM sperm fertilised
eggs at rates equivalent to fertilisation by FM sperm, the larvae produced by VM sperm were significantly smaller at hatching.
Larval length tended to increase in parallel with the duration of sperm motility, but the relationship was not significant
in these tests. The results did not indicate any age or size pattern to spawning readiness in male herring. Sperm that are
not yet ready to be shed are not fully motile on contact with seawater, but are still capable of fertilising eggs that hatch
successfully. There is likely to be a progression of males which come into spawning readiness within a spawning shoal; therefore
it is possible that paternal influences would result in a progressive decrease in larval size over the spawning period in
winter-spawning Celtic Sea herring.
Received: 22 November 1997 / Accepted: 8 June 1998 相似文献
518.
The sibling species, Pseudocalanus moultoni (Frost, 1989) and P. newmani (Frost, 1989), occur sympatrically on Georges Bank. Taxonomic discrimination of the species relies on subtle morphological
characteristics, making routine identification of the species very difficult. DNA sequence variation of two mitochondrial
genes, 16S rRNA and cytochrome oxidase I (COI), reliably discriminated P. moultoni and P.␣newmani. Levels of DNA sequence variation for both genes were consistent with those between species of calanoid copepods. A molecular
systematic protocol (based on allele-specific PCR amplification) was designed from the COI sequences and used to discriminate
females of the two species. The distributions and relative abundances of the two species were mapped for April 1996 based
on samples of 15 to 30 females from 12 collections across Georges Bank. The results of this study indicated that P. moultoni females predominated along the northern flank of Georges Bank, while P. newmani females were common on the southern flank, deeper than the 60-m isobath.
Received: 16 May 1997 / Accepted: 26 April 1998 相似文献
519.
Recovery of benthic communities at Macquarie Island (sub-Antarctic) following a small oil spill 总被引:5,自引:0,他引:5
Intertidal communities at Macquarie Island (sub-Antarctic) were re-evaluated seven years after a small oil spill resulting
from the grounding of the “Nella Dan”, and six years after the first assessment of biological impact. Sampling was conducted
to evaluate community structure in three zones of the exposed rocky shore (upper red, kelp, and lower red) and in samples
of Durvillaea antarctica holdfasts. There were no significant differences between the community structure in oiled and control locations in any of
the three shore zones, but holdfast macrofaunal communities at oiled sites still showed evidence of impact. Holdfast community-structure
in samples from heavily oiled sites showed moderate levels of recovery, with increased abundances of species which were considered
sensitive to the oiling in the first post-impact studies and decreased abundances of opportunistic polychaete and oligochaete
worms. In contrast, samples collected from the moderately oiled location at Secluded Bay showed little evidence of recovery.
Holdfasts at this site were filled with sediment containing traces of diesel oil, and the macrofaunal community was dominated
by opportunistic worms. This study clearly indicates that even small incidents of anthropogenic perturbation can have long-lasting
consequences for marine communities at Macquarie Island.
Received: 23 July 1997 / Accepted: 5 February 1998 相似文献
520.
Reproduction in the sea urchin Centrostephanus rodgersii was examined in two types of habitats (“barrens”, i.e. habitats characterised by the high crustose coralline algal cover
typical of urchin-barren grounds, and by the absence of macroalgae; and “fringe”, i.e. habitats characterised by a high macroalgal
biomass and few C. rodgersii) at four locations in New South Wales. The four locations: the Solitary Islands, Sydney, Ulladulla and Eden, span the distribution
of C.␣rodgersii from the subtropics at its northern limit to temperate waters near its southern limit. Histology and estimates of gonad retrieval
rate (GRR) from January 1994 to October 1995 indicated that reproduction was synchronous at all locations. An increase in
the tempo of gametogenesis in May and onset of spawning in June at all locations is consistent with entrainment in response
to exogenous factors. Over the range studied, C. rodgersii experienced relatively similar daylength cycles and contrasting sea-temperature cycles. Short days and lunar conditions coinciding
with the solstice appear likely proximate cues for the onset of spawning. The major difference in reproduction among locations
was in the duration of spawning. In the southern parts of its range breeding occurred over a 5 to 6 mo period, whereas at
the Solitary Islands it lasted ≃1 mo. At most locations the GRRs were significantly higher in the fringe habitat than in the
barrens habitat. The lower reproductive output of urchins in the barrens habitat was attributed to the food-poor conditions
typical of this habitat. The developing fishery for C. rodgersii is likely to be most effective from March to early May. Urchins from barrens areas may not provide sufficient yield to warrant
harvesting.
Received: 29 October 1997 / Accepted: 18 May 1998 相似文献