Interaction of tebuconazole with bovine serum albumin: determination of the binding mechanism and binding site by spectroscopic methods

Abstract

This study investigates the interaction between tebuconazole and bovine serum albumin (BSA) in a physiological buffer (pH = 7.4) using the fluorescence quenching method to obtain the apparent binding constants (K) and number of binding sites (n) in the interaction between tebuconazole and BSA. The results revealed that tebuconazole can quench the intrinsic fluorescence of BSA through a static quenching procedure. It also shows that the thermodynamic parameters of enthalpy change (ΔH) and entropy change (ΔS) are negative, indicating that the interaction of tebuconazole with BSA is mainly driven by van der Waals forces and hydrogen bonds. The process of binding was a spontaneous process in which Gibbs free energy change was negative. The distance of r between the donor (BSA) and acceptor (tebuconazole) was calculated to be 0.68?nm based on Forster’s non-radiative energy transfer theory. Analysis of synchronous fluorescence, three-dimensional fluorescence and circular dichroism (CD) spectra demonstrates that tebuconazole can induce conformational changes of BSA.     

宁夏毛乌素沙地3种常用固沙植物种群点格局分析

在宁夏盐池毛乌素沙地选取50 m×50 m规格的典型无明显结皮的半固定沙地和有明显结皮的固定沙地各1块,应用Ripley＇s K函数的双变量点格局分析方法,对飞播条件下混播区油蒿(Artemisia ordosica)、羊柴(Hedysarum laeve)和花棒(Hedysarurn scoparium)3种常用治沙植物种内和种间的空间关联性进行了研究.结果表明,3种飞播植物在不同的演替阶段基本上以集群分布为主要特征,在尺度为0～25m范围内,单种分布格局均出现了最大集聚强度.如果实施单独飞播,油蒿密度不宜过高,以集聚强度高的集群分布形式为最佳,羊柴以集聚强度低的集群分布形式为最佳,花棒由于其散生的特性而不适合单独飞播.油蒿和羊柴之间空间关联性呈显著负相关关系,故油蒿和羊柴不适合大面积高密度混播;羊柴和花棒之间呈正相关关系,因此以羊柴为主混播一定比例的花棒效果较好;花棒和油蒿不适合同时混播,但是可将油蒿飞播到有大花棒生长的沙地中.     

新疆极端降水概率分布特征的时空演变规律

依据新疆地区53个雨量站1957-2009年日降水资料,根据研究需要,定义了8个极端降水指标。运用K-S法确定降水指标最适概率分布函数,确定10年一遇极端降水量值;在此基础上,采用Copula非参数估计方法,通过Akaike information criterion(AIC)法确定两降水指标联合分布函数,系统分析1980年以后极端降水单变量极值及降水极值二维联合概率分布特征变化的时空演变特征,研究结果表明:①新疆有湿润化趋势,北疆湿润化趋势比南疆显著;②从降水天数和极端降水天数角度来看,新疆极端强弱降水同时出现的概率减小,从极端降水雨量和强度来看新疆中部、南疆西部、北疆东部及北部极端强弱降水同时出现的可能性减小,而天山西部与南疆北部的可能性增加;③1980年后新疆地区发生涝的概率增大,发生旱的概率减少。     

Effects of high dose copper on plant growth and mineral nutrient (Zn,Fe, Mg,K, Ca) uptake in spinach

Loessal soil is one of the main cultivated soils in northwest China. Part of its distribution area was irrigated with industrial wastewater in past three decades. This caused heavy metal contamination in the soil. It had induced toxicity on crops and also threatened local human health for now. Based on a field plot experiment, effects of different Cu concentrations (from 45 to 2000 mg kg^?1) in loessal soil on spinach plant growth and uptake of mineral nutrients (Zn, Fe, Mg, K, and Ca) by spinach were investigated. The Cu addition increased available concentrations of mineral nutrients in loessal soil and concentrations of Cu, Zn, Mg, and Ca in roots. The translocation of mineral nutrients from roots to leaves was inhibited under Cu addition, inducing their decrease in leaves. The EC₁₀ and EC₅₀ of soil Cu in relative dry weights of leaves were 240.33 mg kg^?1 and 1205.04 mg kg^?1, respectively. The PLS-PM analysis showed that available concentrations of nutrients in soil were only affected by Cu in soil positively, nutrients in roots were mainly affected by Cu in soil and Cu in leaves positively, nutrients in leaves were mainly affected by Cu in roots negatively, translocation of nutrients in spinach and plant growth were principally affected by Cu in leaves negatively, and the total effect of Cu in leaves on nutrients in roots and leaves, translocation of nutrients in spinach, and plant growth was the highest. Our results indicated that the phytotoxicity of Cu including spinach growth inhibition and mineral disorder in spinach was mainly affected by the Cu concentrations in leaves.

     

河流污染原因分析及管理对策

河流污染主要是指污染物直接或间接地通过地表径流或其他方式进入河流而引起河流自净能力和环境功能下降的现象,主要表现为水体黑臭和富营养化。目前,随着城镇化、工业化进程的加快,人口的急剧增长,导致我国河流污染日益严重。河流污染程度与净流量、产业结构及人口规模密切相关。坚持走可持续发展道路,转变先污染后治理的传统观点,积极落实总量控制制度,彻底解决水环境污染问题。     

Chemical composition, mass closure and sources of atmospheric PM10 from industrial sites in Shenzhen, China

Concentrations of atmospheric PM10 and chemical components (including twenty-one elements, nine ions, organic carbon (OC) and elemental carbon (EC)) were measured at five sites in a heavily industrial region of Shenzhen, China in 2005. Results showed that PM10 concentrations exhibited the highest values at 264 μg/m3 at the site near a harbor with the influence of harbor activities. Sulfur exhibited the highest concentrations (from 2419 to 3995 ng/m3) of all the studied elements, which may be related to the influence of coal used as fuel in this area for industrial plants. This was verified by the high mass percentages of SO42-, which accounted for 34.3%-39.7% of the total ions. NO3-/SO42- ratios varied from 0.64-0.71, which implies coal combustion was predominant compared with vehicle emission. The anion/cation ratios range was close to 0.95, indicating anion deficiency in this region. The harbor site showed the highest OC and EC concentrations, with the influence of emission from vessels. Secondary organic carbon accounted for about 22.6%-38.7% of OC, with the highest percentage occurring at the site adjacent to a coal-fired power plant and wood plant. The mass closure model performed well in this heavily industrial region, with significant correlation obtained between chemically determined and gravimetrically measured PM10 mass. The main constituents of PM10 were found to be organic materials (30.9%-69.5%), followed by secondary inorganic aerosol (7.9%-25.0%), crustal materials (6.7%-13.8%), elemental carbon (3.5%-10.8%), sea salt (2.4%-6.2%) and trace elements (2.0%-4.9%) in this heavily industrialized region. Principal component analysis indicated that the main sources for particulate matter in this industrial region were crustal materials and coal/wood combustion, oil combustion, secondary aerosols, industrial processes and vehicle emission.     

Effect of two biogas residues’ application on copper and zinc fractionation and release in different soils

Biogas residue (BR) is widely used as a new green fertilizer in agriculture in China. However, it often contains a high concentration of heavy metals so its application should cause our concern. An incubation experiment was conducted to study the risk of pig biogas residue (PBR) and chicken biogas residue (CBR) application on Liuminying soil (LS) and Yixing soil (YS). The soils were incubated for one, three and six months with 0, 2%, 4% and 6% addition of BRs. According to BCR extraction results, the PBR and CBR applications induced an increase in the concentration of exchangeable fraction of Zn. As for the concentration of exchangeable fraction of Cu, an increase was only observed in the treatments with PBR application. The heavy metal binding intensity also showed a similar trend. With the PBR application, for the LS and YS, the highest concentrations of exchangeable Zn increased 3.6 and 9.5 times, respectively, while the exchangeable Cu was increased by 52.6% and 187.1%. Dissolved organic carbon was the limiting factor for the exchangeable Cu while the exchangeable Zn was controlled by soil pH. PBR presented more agricultural risk than CBR when used as fertilizer. Meanwhile, BRs were more adaptable to LS than YS according to the heavy metal release results.     

Numerical study of regional environmental carrying capacity for livestock and poultry farming based on planting-breeding balance

In consideration of the need to maintain planting-breeding balance, this article examines the capacity of the soil in Putian City, Fujian Province to absorb livestock and poultry excreta, and computes the environmental carrying capacity for livestock and poultry farming (ECCLPF) in each district of the city in terms of the fertility characteristics of the soil in the city, as well as its mix of crops cultivated and farming methods. On the basis of the computations, this work proceeds to classify the alarm grades of the city’s environmental carrying capacity for livestock and poultry framing, and assess the environmental impact of the livestock and poultry farming industry. The results of our study indicate that, the city’s ECCLPF ranges from 8.27 to 23.23 heads per ha when computed on the basis of nitrogen, and from 5.79 to 24.53 heads per ha when computed on the basis of phosphorus. A comparison between our research findings and the existing farming scale in Putian reveals that, in certain parts of the city, ECCLPF is overburdened to varying degrees. Specifically, Chengxiang District is severely overburdened, Hanjiang District and Meizhou Island have a level of overburdening between virtual overburdening and significant overburdening, Licheng District is virtually overburdened, and Xiuyu, Xianyou, and Bei’an Districts have not exceeded their environmental carrying capacity and therefore have varying levels of potential for growth.     

Effects of atamp-charging coke making on strength and high temperature thermal properties of coke

The stamp-charging coke making process has some advantages of improving the operation environment, decreasing fugitive emission, higher gas collection efficiency as well as less environmental pollution. This article describes the different structure strength and high temperature thermal properties of 4 different types of coke manufactured using a conventional coking process and the stamp-charging coke making process. The 4 kinds of cokes were prepared from the mixture of five feed coals blended by the petrography blending method. The results showed that the structure strength indices of coke prepared using the stamp-charging coke method increase sharply. In contrast with conventional coking process, the stamp-charging process improved the coke strength after reaction but had little impact on the coke reactivity index.