Continuous water quality monitoring for the hard clam industry in Florida, USA

In 2000, Florida's fast-growing hard clam aquaculture industry became eligible for federal agricultural crop insurance through the US Department of Agriculture, but the responsibility for identifying the cause of mortality remained with the grower. Here we describe the continuous water quality monitoring system used to monitor hard clam aquaculture areas in Florida and show examples of the data collected with the system. Systems recording temperature, salinity, dissolved oxygen, water depth, turbidity and chlorophyll at 30 min intervals were installed at 10 aquaculture lease areas along Florida's Gulf and Atlantic coasts. Six of these systems sent data in real-time to a public website, and all 10 systems provided data for web-accessible archives. The systems documented environmental conditions that could negatively impact clam survival and productivity and identified biologically relevant water quality differences among clam aquaculture areas. Both the real-time and archived data were used widely by clam growers and nursery managers to make management decisions and in filing crop loss insurance claims. While the systems were labor and time intensive, we recommend adjustments that could reduce costs and staff time requirements.     

SiB3模式对作物区CO2通量的模拟研究

以苏州东山观测站农田为对象,通过使用简单生物圈模式(SiB3),在苏州东山站选取不同植被类型作为模式输入参数进行CO2通量的模拟,并利用苏州东山站2010-04-16~2011-06-30日的观测数据进行对比,讨论不同植被对苏州东山站CO2通量的影响.结果发现,选择玉米作为站点植被类型输入模式模拟的各月CO2通量日变化趋势与观测数据比较一致;而选择普通作物类型输入模式模拟获得的CO2通量,在4月和5月,白天低估CO2通量,6月高估CO2通量;选择茶树类型作为模式植被类型输入的模拟结果,高估5月和6月白天的CO2通量.另外,本研究还使用SiB3模式对每日CO2通量进行模拟,与观测对比后发现,选择的3种植被类型模拟获得的结果能较准确模拟出每日CO2通量的收支,但存在一定偏差.这说明,选择正确的植被类型能够使SiB3模式有效地模拟出CO2通量的日变化,但对时间从小时上升到每日尺度的CO2通量进行模拟时还存在一定困难,需要进一步验证.     

Enhanced phytoextraction of Pb and other metals from artificially contaminated soils through the combined application of EDTA and EDDS

Chemically enhanced phytoextraction is achieved by the application of chelates to soils. Using pot experiments, the effect of the combined application of EDTA and EDDS on the uptake of Cu, Pb, Zn and Cd by Zea mays L. was studied. Among the tested application ratios of 1:1, 1:2, and 2:1 (EDTA/EDDS), 2:1 of EDTA:EDDS was the most efficient ratio for increasing the concentrations of Cu, Pb, Zn and Cd in the shoots. The combined application of 3.33 mmol kg(-1) soil of EDTA+1.67 mmol kg(-1) soil of EDDS produced 650 mg kg(-1) of Pb in the shoots, which was 2.4 and 5.9 times the concentration of Pb in the shoots treated with 5 mmol kg(-1) of EDTA and EDDS alone, respectively. The total phytoextraction of Pb reached 1710 microg kg(-1) soil, which was 2.1 and 6.1 times the total Pb from 5 mmol kg(-1) EDTA and EDDS alone, respectively. The combined application of EDTA and EDDS also significantly increased the translocation of Pb from the roots to the shoots. The mechanism of enhancing the phytoextraction of Pb by the combined application of EDTA+EDDS did not involve a change in the pH of the soil. The increase in the phytoextraction of Pb by the shoots of Z. mays L. was more pronounced than the increase of Pb in the soil solution with the combined application of EDTA and EDDS. It was thought that the major role of EDDS might be to increase the uptake and translocation of Pb from the roots to the shoots of plants.     

Fluorescence characterization of cross flow ultrafiltration derived freshwater colloidal and dissolved organic matter

3-D fluorescence excitation-emission matrix (EEM) spectrophotometry was applied to investigate the fluorescence characterization of colloidal organic matter (COM) and truly dissolved organic matter (DOM) from an urban lake and a rural river fractionated by the cross flow ultrafiltration (CFUF) process with a 1kDa membrane. Relatively high tryptophan-like fluorescence intensity is found in the urban water, although the fluorescence of both water samples is mainly dominated by humic/fulvic-like fluorophores. During CFUF processing, the fluorescence intensities of humic/fulvic-like materials in the retentate increased rapidly, but a slight increase is also observed in the permeate fluorescence intensity. Very different ultrafiltration behaviour occurred with respect to the tryptophan-like fluorophore, where both permeate and retentate fluorescence intensities increase substantially at the beginning of the CFUF process, then tend to remain constant at high concentration factor (cf) values. Comparison with tryptophan standards demonstrates that freshwater tryptophan-like fluorescence is not dissolved and 'free', but is, in part, colloidal and related to the ultrafiltration behaviour of fulvic/humic-like matter. A good linear relationship between the retentate humic/fulvic-like fluorescence intensity and organic carbon concentration further reveals that fluorescent humic/fulvic-like substances are the dominant contributors to colloidal organic carbon, mainly in the colloidal fraction.     

Soil Cd availability to Indian mustard and environmental risk following EDTA addition to Cd-contaminated soil

A pot experiment was conducted to investigate the influence of EDTA on the extractability of Cd in the soil and uptake of Cd by Indian mustard (Brassica juncea). Twenty levels of soil Cd concentration ranging from 10 to 200 mg kg(-1) were produced by spiking aliquots of a clay loam paddy soil with Cd(NO3)2. One week before the plants were harvested EDTA was applied to pots in which the soil had been spiked with 20, 40, 60...200 mg Cd kg(-1). The EDTA was added at the rate calculated to complex with all of the Cd added at the 200 mg kg(-1) level. Control pots spiked with 10, 30, 50... 190 mg Cd kg(-1) received no EDTA. The plants were harvested after 42 days' growth. Soil water- and NH4NO3-extractable Cd fractions increased rapidly following EDTA application. Root Cd concentrations decreased after EDTA application, but shoot concentrations increased when the soil Cd levels were >130 mg kg(-1) and Cd toxicity symptoms were observed. The increases in soil solution Cd induced by EDTA did not increase plant total Cd uptake but appeared to stimulate the translocation of the metal from roots to shoots when the plants appeared to be under Cd toxicity stress. The results are discussed in relation to the possible mechanisms by which EDTA may change the solubility and bioavailability of Cd in the soil and the potential for plant uptake and environmental risk due to leaching losses to groundwater.     

Phytoextraction of metals from a multiply contaminated soil by Indian mustard

The effects of nitrilotriacetate (NTA) and citric acid applications on metal extractability from a multiply metal-contaminated soil, as well as on their uptake and accumulation by Indian mustard (Brassica juncea) were investigated. Desorption of metals from the soil increased with chelate concentration, NTA being more effective than citric acid in solubilising the metals. Plants were grown in a sandy soil collected from a contaminated field site and polluted by Cd, Cr, Cu, Pb and Zn. After 43 days of plant growth, pots were amended with NTA or citric acid at 5 mmol kg-1 soil. Control pots were not treated with any chelate. Harvest of plants was performed 1 week after chelate addition. Soil water-, NH4NO3- and DTPA-extractable Cd, Cu, Pb and Zn fractions were enhanced only in the presence of NTA. In comparison to unamended plants, Indian mustard shoot dry weights suffered significant reductions following NTA application. NTA treatment increased shoot metal concentrations by a factor of 2-3, whereas citric acid did not induce any difference compared to the control. Chromium was detected in the above-ground tissues only after NTA amendment. Due to differences in dry matter yield, a significant enhancement of metal uptake was observed in NTA-treated plants for Cu and Zn.     

Understanding complex drivers of wildlife crime to design effective conservation interventions

In conservation understanding the drivers of behavior and developing robust interventions to promote behavioral change is challenging and requires a multifaceted approach. This is particularly true for efforts to address illegal wildlife use, where pervasive—and sometimes simplistic—narratives often obscure complex realities. We used an indirect questioning approach, the unmatched count technique, to investigate the drivers and prevalence of wildlife crime in communities surrounding 2 national parks in Uganda and combined scenario interviews and a choice experiment to predict the performance of potential interventions designed to tackle these crimes. Although poverty is often assumed to be a key driver of wildlife crime, we found that better-off households and those subject to human–wildlife conflict and those that do not receive any benefits from the parks’ tourism revenue sharing were more likely to be involved in certain types of wildlife crime, especially illegal hunting. The interventions predicted to have the greatest impact on reducing local participation in wildlife crime were those that directly addressed the drivers including, mitigating damage caused by wildlife and generating financial benefits for park-adjacent households. Our triangulated approach provided insights into complex and hard-to-access behaviors and highlighted the importance of going beyond single-driver narratives.     

A comprehensive evaluation of the potential chemical defenses of antarctic ascidians against sympatric fouling microorganisms

The present study analyzed the bioactivity of whole body extracts from six solitary and eight colonial ascidian taxa against 20 sympatric bacterial isolates and one sympatric diatom species from the Western Antarctic Peninsula. Ascidians had crude lipophilic and hydrophilic extracts assayed against 20 bacterial strains. The lipophilic extract of one ascidian caused growth inhibition in all bacterial isolates at 3× tissue-level concentrations. The lipophilic and hydrophilic extracts were fractionated into seawater-soluble and insoluble fractions and assayed at three concentrations against a sympatric diatom species. Significant diatom mortality was detected at 3× and 1× concentrations in all but one ascidian taxon. Lipophilic fractions caused higher diatom mortality than hydrophilic extracts. The specificity of secondary metabolites against diatom fouling and the lack of activity against bacteria suggest high selective pressure for chemical defenses against diatom fouling or the potential that bacterial pathogens are controlled by the ascidian immune system.     

USING FIELD SCALE MODELS TO PREDICT PEAK FLOWS ON AGRICULTURAL WATERSHEDS1

The goal of this study was to develop a methodology for generating storm hydrographs at a watershed scale based on daily runoff estimates from a field scale model. The methodology was evaluated on a small agricultural watershed using the ADAPT field scale process model. A comparison of observed and predicted peak flows for 11 of the largest events that occurred in a three year period gave r² values of 0.84, 0.82, and 0.81 when the watershed was subdivided into 1, 5, and 10 sub watersheds. However, all other statistical measures improved when the watershed was subdivided into at least five sub watersheds. Guidelines need to be developed on the use of the procedure but it first needs to be evaluated on several watersheds that exhibit a range in sizes, land uses, slopes, and soil properties.