Monoterpenes and microbial metabolites in the soil

Volatile microbial metabolites were analysed in the soil beneath 15 H year-old Picea abies which had been fumigated for 5 consecutive years with charcoal-filtered air enriched with ozone and sulphur dioxide. Major compounds which could be attributed to the metabolism of basidiomycete fungi were oct-1-en-3-ol, oct-1-en-3-one and 1,3-octadiene. They were accompanied by other C(8)-compounds. The lower amounts of C(8)-compounds observed in the soil of the sulphur dioxide and ozone treatment chambers indicate a reduced development of basidiomycetes. The higher amounts of geosmin and 2-methylisoborneol found in the soil of the ozone treatment chamber indicate an increase in actinomycete populations. The differences of the monoterpene patterns in the particular soils were not large enough to recognize trends unequivocally.     

Field desorption of ions from electrolytic droplets

The Science of Nature -     

Investment and future mineral production

The author reviews recent estimates of world resources and reserves and notes that reserves tend to grow with mineral consumption. Legal and economic constraints to the extraction of minerals are at least as important as physical availability. The author also points out that there is far more investment in minerals (government induced investment, companies diversifying into mining, etc) than some commentators suggest. However, the large mining projects of the 1960s belong to the past, and supplies of minerals may well become tight if there is a concerted economic boom.     

A study of reproduction and other life cycle phenomena in planktonic protists using an acridine orange fluorescence technique

The percentage of dividing individuals and temporal reproductive patterns were determined for natural populations of several planktonic protists including five species of tintinnids, a dinoflagellate, and a diatom. To obtain these data, a method was used in which the nuclei of planktonic ciliates and phytoplankters can be fluorescently stained with acridine orange at the time of collection and fixation. The technique is simple and can be used routinely in studies of reproduction or other life cycle phenomena of natural protistan populations. For the tintinnids, often more than half of the individuals were in some recognizable stage of fission; periodicity in the division process was only observed once and apparently followed a pulse of conjugation in the population. With the diatom Ditylum brightwellii the fluorescent staining technique yielded data on the extent and timing of division which were consistent with, but more complete than, previous enumerations of paired cells.The support of the National Science Foundation (OCE-7819566) is acknowledged     

Importance of physical and biological settlement cues used at different spatial scales by the larvae of Semibalanus balanoides

The settling cues used by larvae of Semibalanus balanoides (L.) were examined at large (1 m), medium (1 mm), and small (<300 m) spatial scales, corresponding roughly to choices made during broad and close exploration and final inspection within the settling zone. The experiments were carried out at two locations in Canada in May and June 1984. Samples of substratum surfaces from above (n=87 sites unselected by cyprids) and below (n=84 sites selected by cyprids) the upper limit of the barnacle zone were characterized according to 13 quantitative and semi-quantitative variables (diatom cover, macroalgae, detritus) using scanning electron microscopy (SEM). The data were analysed using a stepwise logistic regression. At the large scale, the presence of an alga (Urospora wormskjoldii Mertens) in the upper uncolonized zone and a diatom (Achnantes parvula Kützing) within the settling zone are potential settlement cues. At the medium scale in the settling zone, larvae select clean sites, devoid of detritus and diatoms or on which detrital matter, when present, is of finer texture than on the unchosen sites within the settling zone. At the smallest scale (<300 m, equal to the average maximum width of the larvae), we tested the hypothesis that the larva seeks optimal microheterogeneity (presumably providing good adhesion). Microheterogeneity of the surface was measured immediately under the larva, around the settling organs, the antennules. Our results show that such surface microheterogeneity is significantly greater and the vertical center of gravity of the surface significantly lower on the sites selected by the cyprids than on the unselected contiguous sites. Fractal measurements carried out on selected and contiguous unselected surfaces indicate that larvae discriminate microheterogeneity levels below a step length of 35 m, a value approximately the size of the diameter of the antennular discs (40 m), which are used to explore the substratum and attach.Contribution to the programme of GIROQ (Groupe interuniversitaire de recherches océanographiques du Québec)