An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters

The applicability of microarrays to monitor harmful algae across a broad range of ecological niches and toxic species responsible for harmful algal events has been one of the key tasks in the EU Seventh Framework Programme (FP7)-funded Microarrays for the Detection of Toxic Algae project. The technique has a strong potential for improving speed and accuracy of the identification of harmful algae and their toxins to assist monitoring programmes. Water samples were collected from a number of coastal sites around Ireland, including several that are used in the Irish National Phytoplankton and Biotoxin Monitoring Programme. Ribosomal RNA was extracted from filtered field samples, labelled with a fluorescent dye, and hybridised to probes spotted in a microarray format on a glass slide. The fluorescent signal intensity of the hybridisation to >120 probes on the chip was analysed and compared with actual field counts. There was a general agreement between cell counts and microarray signal. Results are presented for field samples taken from a range of stations along the Irish coastline known for harmful algal events during the first field trial (July 2009–April 2010).     

污水二氧化氯和氯消毒过程中遗传毒性的变化及氨氮的影响

采用umu遗传毒性测试方法考察了二氧化氯和氯消毒对几种城市污水生物处理出水遗传毒性的影响,发现当二氧化氯消毒剂从0　mg/L增加到30　mg/L时,几种污水的遗传毒性均先迅速降低后趋于稳定,而当氯消毒剂从0　mg/L增加到30　mg/L时,几种污水的遗传毒性的变化规律不同.进一步研究氨氮对污水消毒过程中遗传毒性变化的影响,发现氨氮对污水二氧化氯消毒过程中遗传毒性的变化规律没有显著影响,但是对污水氯消毒过程中遗传毒性的变化规律却起着至关重要的作用.当氨氮含量较小（<10～20　mg/L）时,污水氯消毒后的遗传毒性小于消毒前;当氨氮含量较大时（>10～20　mg/L）,污水氯消毒后的遗传毒性大于消毒前.