Chemistry of Selected High-Elevation Lakes in Seven National Parks in the Western United States

A chemical survey of 69 high-altitude lakes in seven national parks in the western United States was conducted during the fallof 1999; the lakes were previously sampled during the fall of 1985, as part of the Western Lake Survey. Lakes in parks in the Sierra/southern Cascades (Lassen Volcanic, Yosemite, Sequoia/Kings Canyon National Parks) and in the southern RockyMountains (Rocky Mountain National Park) were very dilute; medianspecific conductance ranged from 4.4 to 12.2 S cm^-1 andmedian alkalinity concentrations ranged from 32.2 to 72.9 eqL^-1. Specific conductances and alkalinity concentrations were substantially higher in lakes in the central and northernRocky Mountains parks (Grand Teton, Yellowstone, and GlacierNational Parks), probably due to the prevalence of more reactivebedrock types. Regional patterns in lake concentrations of NO₃ and SO₄ were similar to regional patterns in NO₃ and SO₄ concentrations in precipitation, suggestingthat the lakes are showing a response to atmospheric deposition.Concentrations of NO₃ were particularly high in Rocky Mountain National Park, where some ecosystems appear to be undergoing nitrogen saturation.     

Cadmium accumulation and metallothionein synthesis in freshwater bivalves (Pyganodon grandis): relative influence of the metal exposure gradient versus limnological variability

The relative influence of limnological confounding factors on cadmium (Cd) bioaccumulation and metallothionein (MT) synthesis was quantified in natural populations of freshwater bivalves (Pyganodon grandis) living in lakes along a Cd concentration gradient. During the ice-free period, we measured 15 environmental variables in the water compartment and determined total concentrations of Cd and MT in the gills of bivalves at 37 littoral stations in 20 lakes distributed across the mining area of Rouyn-Noranda in northwestern Quebec. A multiple linear regression model including pH (+), dissolved Ca concentrations (-) and free Cd2+ concentrations at the sediment-water interface (+) explained 74% of the variability in Cd concentrations in the bivalve gills. Dissolved Ca (-) and free Cd2+ (+) together explained 62% of the variation in MT concentrations in the bivalve gills. Partial linear regression analyses indicated that the limnological factors' pure and shared effects together accounted for 48 and 45% of the total variation in Cd and MT concentrations in the gills, respectively. A lake selection procedure that could be applied in monitoring programs is proposed to minimise the relative influence of these confounding variables.     

Patterns of nitrogenous waste excretion and gill urea transporter mRNA expression in several species of marine fish

Many prior studies of nitrogenous waste excretion in marine fish have examined excretion patterns for short time periods, and with relatively coarse sampling schemes (e.g., an initial and a final sample point). Recent studies of a ureotelic marine fish (the gulf toadfish, Opsanus beta) have demonstrated that urea excretion in this species occurs in brief but massive bursts, lasting from 0.5 to 3 h, and often only once per day. The present study sought to determine if prior sampling protocols may have underestimated the amount of urea being excreted by marine fish. A survey of 16 marine species (the teleosts: Myoxocephalus octodecemspinosus, Scophthalamus aquosa, Cyclopterus lumpus, Lophius americanus, Aprodon cortezianus, Cymatogaster aggregatus, Parophrys vetulis, Microstomus pacificus, Hippoglossoides elassodon, Bathyagonus nigripinnus, Ophiodon elongatus, Hemilepidatus spinosus, Icelinus terrius; the elasmobranch: Raja rhina; and the hagfish: Eptatretus stoutii) was undertaken for ammonia-N and urea-N excretion using a long sampling period (48 h) and hourly sample collection. Apart from the obvious exception of an elasmobranch, ammonia excretion was confirmed to be predominant in marine fish, with urea excretion constituting between 1.4 and 23.8% of the total of ammonia plus urea excreted. Notably, no pulses of urea excretion were detected. Despite the relatively low level of urea excretion, expression of urea transporter-like mRNA (detected using the toadfish urea transporter, tUT, cDNA as a probe) was discovered in gills of many of the species surveyed for nitrogen excretion patterns, although no signal was detected in the hagfish. These results suggest that urea excretion takes place through a specific transport pathway. Finally, more detailed analysis of nitrogen excretion in one of the surveyed species, the plainfin midshipman (Porichthys notatus) demonstrates that "total" nitrogen excretion estimated by summing ammonia and urea excretion underestimates true total nitrogen excretion by 37-51%.     

The effect of EDTA and fulvic acid on Cd, Zn, and Cu toxicity to a bioluminescent construct (pUCD607) of Escherichia coli

The hypothesis, that metal toxicity is dominated by free ion activity, was tested by comparing calculated metal activities with measured toxic responses to a genetically modified, luminescent bacterium, Escherichia coli. The toxicity of Cd, Cu, and Zn sulphate salts in the presence of EDTA and fulvic acid in well-defined solutions was measured. Good agreement between free metal activity and toxicity was found for Cu but not for Zn and Cd. The toxicity relationships were altered by glucose addition to the organism. Stable chloride complexes may have contributed to the toxicity of Cd under the test conditions. The results suggest that there is not always a simple relationship between toxicity and free-ion metal concentration and that further account should be taken of competitive interactions between living cells and ligands and the physiological status of the organism.     

Time series in M dimensions: The one-dimensional case: Pollution in the Chicago Sanitary and Ship Canal

A nonstationary time-series model is used to examine the changes occurring at sampling stations on the Chicago Sanitary and Ship Canal. Using data from upstream sampling sites, downstream levels of dissolved oxygen, total dissolved solids, nitrates and nitrites, and ammonia are accurately predicted. The method is simple, insensitive to extreme values, and responsive to changes in the system.     

Implications of geometric plasticity for maximizing photosynthesis in branching corals

Reef-building corals are an example of plastic photosynthetic organisms that occupy environments of high spatiotemporal variations in incident irradiance. Many phototrophs use a range of photoacclimatory mechanisms to optimize light levels reaching the photosynthetic units within the cells. In this study, we set out to determine whether phenotypic plasticity in branching corals across light habitats optimizes potential light utilization and photosynthesis. In order to do this, we mapped incident light levels across coral surfaces in branching corals and measured the photosynthetic capacity across various within-colony surfaces. Based on the field data and modelled frequency distribution of within-colony surface light levels, our results show that branching corals are substantially self-shaded at both 5 and 18 m, and the modal light level for the within-colony surface is 50 μmol photons m^?2 s^?1. Light profiles across different locations showed that the lowest attenuation at both depths was found on the inner surface of the outermost branches, while the most self-shading surface was on the bottom side of these branches. In contrast, vertically extended branches in the central part of the colony showed no differences between the sides of branches. The photosynthetic activity at these coral surfaces confirmed that the outermost branches had the greatest change in sun- and shade-adapted surfaces; the inner surfaces had a 50 % greater relative maximum electron transport rate compared to the outer side of the outermost branches. This was further confirmed by sensitivity analysis, showing that branch position was the most influential parameter in estimating whole-colony relative electron transport rate (rETR). As a whole, shallow colonies have double the photosynthetic capacity compared to deep colonies. In terms of phenotypic plasticity potentially optimizing photosynthetic capacity, we found that at 18 m, the present coral colony morphology increased the whole-colony rETR, while at 5 m, the colony morphology decreased potential light utilization and photosynthetic output. This result of potential energy acquisition being underutilized in shallow, highly lit waters due to the shallow type morphology present may represent a trade-off between optimizing light capture and reducing light damage, as this type morphology can perhaps decrease long-term costs of and effect of photoinhibition. This may be an important strategy as opposed to adopting a type morphology, which results in an overall higher energetic acquisition. Conversely, it could also be that maximizing light utilization and potential photosynthetic output is more important in low-light habitats for Acropora humilis.     

Development and Operational Testing of a Super‐Ensemble Artificial Intelligence Flood‐Forecast Model for a Pacific Northwest River

Coastal catchments in British Columbia, Canada, experience a complex mixture of rainfall‐ and snowmelt‐driven contributions to flood events. Few operational flood‐forecast models are available in the region. Here, we integrated a number of proven technologies in a novel way to produce a super‐ensemble forecast system for the Englishman River, a flood‐prone stream on Vancouver Island. This three‐day‐ahead modeling system utilizes up to 42 numerical weather prediction model outputs from the North American Ensemble Forecast System, combined with six artificial neural network‐based streamflow models representing various slightly different system conceptualizations, all of which were trained exclusively on historical high‐flow data. As such, the system combines relatively low model development times and costs with the generation of fully probabilistic forecasts reflecting uncertainty in the simulation of both atmospheric and terrestrial hydrologic dynamics. Results from operational testing by British Columbia's flood forecasting agency during the 2013‐2014 storm season suggest that the prediction system is operationally useful and robust.     

Assessment of vegetation stress using reflectance or fluorescence measurements

Current methods for large-scale vegetation monitoring rely on multispectral remote sensing, which has serious limitation for the detection of vegetation stress. To contribute to the establishment of a generalized spectral approach for vegetation stress detection, this study compares the ability of high-spectral-resolution reflectance (R) and fluorescence (F) foliar measurements to detect vegetation changes associated with common environmental factors affecting plant growth and productivity. To obtain a spectral dataset from a broad range of species and stress conditions, plant material from three experiments was examined, including (i) corn, nitrogen (N) deficiency/excess; (ii) soybean, elevated carbon dioxide, and ozone levels; and (iii) red maple, augmented ultraviolet irradiation. Fluorescence and R spectra (400-800 nm) were measured on the same foliar samples in conjunction with photosynthetic pigments, carbon, and N content. For separation of a wide range of treatment levels, hyperspectral (5-10 nm) R indices were superior compared with F or broadband R indices, with the derivative parameters providing optimal results. For the detection of changes in vegetation physiology, hyperspectral indices can provide a significant improvement over broadband indices. The relationship of treatment levels to R was linear, whereas that to F was curvilinear. Using reflectance measurements, it was not possible to identify the unstressed vegetation condition, which was accomplished in all three experiments using F indices. Large-scale monitoring of vegetation condition and the detection of vegetation stress could be improved by using hyperspectral R and F information, a possible strategy for future remote sensing missions.