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31.
The relative influence of limnological confounding factors on cadmium (Cd) bioaccumulation and metallothionein (MT) synthesis was quantified in natural populations of freshwater bivalves (Pyganodon grandis) living in lakes along a Cd concentration gradient. During the ice-free period, we measured 15 environmental variables in the water compartment and determined total concentrations of Cd and MT in the gills of bivalves at 37 littoral stations in 20 lakes distributed across the mining area of Rouyn-Noranda in northwestern Quebec. A multiple linear regression model including pH (+), dissolved Ca concentrations (-) and free Cd2+ concentrations at the sediment-water interface (+) explained 74% of the variability in Cd concentrations in the bivalve gills. Dissolved Ca (-) and free Cd2+ (+) together explained 62% of the variation in MT concentrations in the bivalve gills. Partial linear regression analyses indicated that the limnological factors' pure and shared effects together accounted for 48 and 45% of the total variation in Cd and MT concentrations in the gills, respectively. A lake selection procedure that could be applied in monitoring programs is proposed to minimise the relative influence of these confounding variables.  相似文献   
32.
Many prior studies of nitrogenous waste excretion in marine fish have examined excretion patterns for short time periods, and with relatively coarse sampling schemes (e.g., an initial and a final sample point). Recent studies of a ureotelic marine fish (the gulf toadfish, Opsanus beta) have demonstrated that urea excretion in this species occurs in brief but massive bursts, lasting from 0.5 to 3 h, and often only once per day. The present study sought to determine if prior sampling protocols may have underestimated the amount of urea being excreted by marine fish. A survey of 16 marine species (the teleosts: Myoxocephalus octodecemspinosus, Scophthalamus aquosa, Cyclopterus lumpus, Lophius americanus, Aprodon cortezianus, Cymatogaster aggregatus, Parophrys vetulis, Microstomus pacificus, Hippoglossoides elassodon, Bathyagonus nigripinnus, Ophiodon elongatus, Hemilepidatus spinosus, Icelinus terrius; the elasmobranch: Raja rhina; and the hagfish: Eptatretus stoutii) was undertaken for ammonia-N and urea-N excretion using a long sampling period (48 h) and hourly sample collection. Apart from the obvious exception of an elasmobranch, ammonia excretion was confirmed to be predominant in marine fish, with urea excretion constituting between 1.4 and 23.8% of the total of ammonia plus urea excreted. Notably, no pulses of urea excretion were detected. Despite the relatively low level of urea excretion, expression of urea transporter-like mRNA (detected using the toadfish urea transporter, tUT, cDNA as a probe) was discovered in gills of many of the species surveyed for nitrogen excretion patterns, although no signal was detected in the hagfish. These results suggest that urea excretion takes place through a specific transport pathway. Finally, more detailed analysis of nitrogen excretion in one of the surveyed species, the plainfin midshipman (Porichthys notatus) demonstrates that "total" nitrogen excretion estimated by summing ammonia and urea excretion underestimates true total nitrogen excretion by 37-51%.  相似文献   
33.
The hypothesis, that metal toxicity is dominated by free ion activity, was tested by comparing calculated metal activities with measured toxic responses to a genetically modified, luminescent bacterium, Escherichia coli. The toxicity of Cd, Cu, and Zn sulphate salts in the presence of EDTA and fulvic acid in well-defined solutions was measured. Good agreement between free metal activity and toxicity was found for Cu but not for Zn and Cd. The toxicity relationships were altered by glucose addition to the organism. Stable chloride complexes may have contributed to the toxicity of Cd under the test conditions. The results suggest that there is not always a simple relationship between toxicity and free-ion metal concentration and that further account should be taken of competitive interactions between living cells and ligands and the physiological status of the organism.  相似文献   
34.
A nonstationary time-series model is used to examine the changes occurring at sampling stations on the Chicago Sanitary and Ship Canal. Using data from upstream sampling sites, downstream levels of dissolved oxygen, total dissolved solids, nitrates and nitrites, and ammonia are accurately predicted. The method is simple, insensitive to extreme values, and responsive to changes in the system.  相似文献   
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Reef-building corals are an example of plastic photosynthetic organisms that occupy environments of high spatiotemporal variations in incident irradiance. Many phototrophs use a range of photoacclimatory mechanisms to optimize light levels reaching the photosynthetic units within the cells. In this study, we set out to determine whether phenotypic plasticity in branching corals across light habitats optimizes potential light utilization and photosynthesis. In order to do this, we mapped incident light levels across coral surfaces in branching corals and measured the photosynthetic capacity across various within-colony surfaces. Based on the field data and modelled frequency distribution of within-colony surface light levels, our results show that branching corals are substantially self-shaded at both 5 and 18 m, and the modal light level for the within-colony surface is 50 μmol photons m?2 s?1. Light profiles across different locations showed that the lowest attenuation at both depths was found on the inner surface of the outermost branches, while the most self-shading surface was on the bottom side of these branches. In contrast, vertically extended branches in the central part of the colony showed no differences between the sides of branches. The photosynthetic activity at these coral surfaces confirmed that the outermost branches had the greatest change in sun- and shade-adapted surfaces; the inner surfaces had a 50 % greater relative maximum electron transport rate compared to the outer side of the outermost branches. This was further confirmed by sensitivity analysis, showing that branch position was the most influential parameter in estimating whole-colony relative electron transport rate (rETR). As a whole, shallow colonies have double the photosynthetic capacity compared to deep colonies. In terms of phenotypic plasticity potentially optimizing photosynthetic capacity, we found that at 18 m, the present coral colony morphology increased the whole-colony rETR, while at 5 m, the colony morphology decreased potential light utilization and photosynthetic output. This result of potential energy acquisition being underutilized in shallow, highly lit waters due to the shallow type morphology present may represent a trade-off between optimizing light capture and reducing light damage, as this type morphology can perhaps decrease long-term costs of and effect of photoinhibition. This may be an important strategy as opposed to adopting a type morphology, which results in an overall higher energetic acquisition. Conversely, it could also be that maximizing light utilization and potential photosynthetic output is more important in low-light habitats for Acropora humilis.  相似文献   
37.
Coastal catchments in British Columbia, Canada, experience a complex mixture of rainfall‐ and snowmelt‐driven contributions to flood events. Few operational flood‐forecast models are available in the region. Here, we integrated a number of proven technologies in a novel way to produce a super‐ensemble forecast system for the Englishman River, a flood‐prone stream on Vancouver Island. This three‐day‐ahead modeling system utilizes up to 42 numerical weather prediction model outputs from the North American Ensemble Forecast System, combined with six artificial neural network‐based streamflow models representing various slightly different system conceptualizations, all of which were trained exclusively on historical high‐flow data. As such, the system combines relatively low model development times and costs with the generation of fully probabilistic forecasts reflecting uncertainty in the simulation of both atmospheric and terrestrial hydrologic dynamics. Results from operational testing by British Columbia's flood forecasting agency during the 2013‐2014 storm season suggest that the prediction system is operationally useful and robust.  相似文献   
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Current methods for large-scale vegetation monitoring rely on multispectral remote sensing, which has serious limitation for the detection of vegetation stress. To contribute to the establishment of a generalized spectral approach for vegetation stress detection, this study compares the ability of high-spectral-resolution reflectance (R) and fluorescence (F) foliar measurements to detect vegetation changes associated with common environmental factors affecting plant growth and productivity. To obtain a spectral dataset from a broad range of species and stress conditions, plant material from three experiments was examined, including (i) corn, nitrogen (N) deficiency/excess; (ii) soybean, elevated carbon dioxide, and ozone levels; and (iii) red maple, augmented ultraviolet irradiation. Fluorescence and R spectra (400-800 nm) were measured on the same foliar samples in conjunction with photosynthetic pigments, carbon, and N content. For separation of a wide range of treatment levels, hyperspectral (5-10 nm) R indices were superior compared with F or broadband R indices, with the derivative parameters providing optimal results. For the detection of changes in vegetation physiology, hyperspectral indices can provide a significant improvement over broadband indices. The relationship of treatment levels to R was linear, whereas that to F was curvilinear. Using reflectance measurements, it was not possible to identify the unstressed vegetation condition, which was accomplished in all three experiments using F indices. Large-scale monitoring of vegetation condition and the detection of vegetation stress could be improved by using hyperspectral R and F information, a possible strategy for future remote sensing missions.  相似文献   
40.
Abstract: Considerable attention has recently been focused on using levels of developmental instability among members of a population to detect environmental or genetic stresses on animals or plants. It is not yet clear, however, that high developmental instability in a sample of individuals always indicates environmental stress or poor genetic quality. We studied 13 fragmented populations of prairie phlox (   Phlox pilosa L.) to test the hypothesis that developmental instability should decrease with increasing population size—as expected if small populations suffer genetic problems associated with inbreeding or are exposed to more environmental stress than larger populations. We used two different measures of developmental instability, each calculated for two different traits: radial asymmetry of flowers (for petal width and petal length) and modular fluctuating asymmetry of leaves (  for leaf widths at two points along the leaf  ). There were weak but significant correlations among individuals for four of six pairwise combinations of these measures. Surprisingly, three of our four measures of developmental instability showed strong population size effects that were opposite to those expected: developmental instability increased with population size. We conclude that measures of developmental instability cannot be applied uncritically for biomonitoring without considerable knowledge of developmental mechanisms, natural history, and population biology of the species in question.  相似文献   
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