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31.
Ghostin J Habib-Jiwan JL Rozenberg R Daloze D Pasteels JM Braekman JC 《Die Naturwissenschaften》2007,94(7):601-605
The adults of the leaf beetle Platyphora kollari (Chrysomelidae) are able to metabolise the oleanane triterpene β-amyrin (1) into the glycoside 3-O-β-d-glucopyranosyl-(1→4)-β-d-glucuronopyranosyl-hederagenin (2) that is stored in their defensive glands. The aim of this study was to test the hypothesis that oleanolic acid (3) is an intermediate in the conversion of 1 into 2 and to check whether the sequestration of pentacyclic triterpenes is selective in favour of β-amyrin (1). To this end, adults of P. kollari were fed with Ipomoea batatas leaf disks painted with a solution of [2,2,3-2H3]oleanolic acid or [2,2,3-2H3]α-amyrin and the secretion of their defensive glands analysed by HPLC–ESIMS. The data presented in this work indicated that
the first step of the transformation of β-amyrin (1) into the sequestered glycoside 2 is its oxidation into oleanolic acid (3) and that this conversion is selective but not specific in favour of β-amyrin (1). 相似文献
32.
Bogers R De Vries-Buitenweg S Geuijen I van de Waart B Kuiper R Van Der Linden S Puijker L Murk A Van Der Burg B Legler J 《Environment international》2007,33(3):292-301
Adult male fathead minnow were exposed for 14 or 28-days under flow-through conditions to undiluted filtered water samples from the rivers Meuse and Rhine in the Netherlands. The experiment included two vessels per treatment each containing 10 fish and samples of five fish were taken after 14 and 28 days. Additional groups were exposed to 17alpha-ethinylestradiol (EE2) as a reference and untreated drinking water as a negative control. Major endpoints examined included induction of vitellogenin (VTG) synthesis, VTG mRNA activity, hepato- and gonadosomatic indices (HSI and GSI) and gonadal histology. No significant difference was recorded in body weight or mean GSI values between the various treatments. Only exposure to Meuse water resulted in significantly higher HSI means after 14 days. Histological examination showed no apparent effects on gonadal tissue except for eosinophilic blood plasma in fish exposed to Meuse water or EE2. After 14 and 28 days, elevated VTG and VTG mRNA levels were measured in most livers of the fish exposed to Meuse water, but not in the fish exposed to Rhine water. This was confirmed by measuring estrogenic responses in the in vitro ER CALUX assay. Induction of VTG synthesis proved to be the most sensitive endpoint in the Non Spawning Male Fish Assay for in vivo detection of bio-available estrogenic activity supplementary to a sensitive in vitro assay. The other endpoints examined varied too much and required a higher number of fish or replicates to achieve sufficient power for statistical testing making them less animal friendly. 相似文献
33.
Ramos Romulo José Tadokoro Carlos Eduardo de Carvalho Gomes Levy Leite Gustavo Rocha 《Environment, Development and Sustainability》2021,23(10):14878-14907
Environment, Development and Sustainability - An abundant population of Ucides cordatus swamp crabs is present at Lameirão Ecological Station (Brazil), a tropical ecosystem man-made with... 相似文献
34.
This work represents an attempt to define a simple method to classify the relative degree of disturbance of sites in lotic
systems on the basis of comparison of their faunistic composition with reference sites. Two ecotypes were selected in northern
Portugal where benthic invertebrates were sampled in reaches with different levels of contamination. As a first stage, previous
Geographic Information System information was used to define reference sites in each ecotype. Afterwards, multivariate techniques
and non linear estimation models were combined to assess biological quality. This method allowed us to quantify sites according
to increasing levels of contamination, after the probabilities of occurrence of taxa along a gradient of contamination taking
into account the reference condition. The results suggest that this method is sensitive to organic pollution, easy to interpret,
namely the species tolerance, and could be a good framework to establish regional rankings depending on the ecological impact
of river sites. 相似文献
35.
A Characterization of Selected Endocrine Disruptor Compounds in a Portuguese Wastewater Treatment Plant 总被引:1,自引:0,他引:1
Maurício R Diniz M Petrovic M Amaral L Peres I Barceló D Santana F 《Environmental monitoring and assessment》2006,118(1-3):75-87
Anthropogenic compounds that are able to disrupt the endocrine system of wildlife species are a major cause for concern and
have led to a demand for new screening methods. The identification and quantification of endocrine disruptor compounds at
wastewater treatment plant is of major interest to assess the endocrine activity of wastewater treatment plant discharges
into the environment. This study consists of a preliminary survey of concentrations of previously selected endocrine disruptor
compounds, undertaken to establish environmental concentrations and to support a biological program assay exposing freshwater
fish to them. Selected endocrine disrupting chemicals (APEs, bisphenol A and 17 β-estradiol) were measured in samples from a wastewater treatment plant located in Lisbon (Portugal), using recent commercial
enzyme-linked immunosorbent assay kits and also LC-MS/MS. The results show that the wastewater treatment plant treatment process
is efficient on the removal of target endocrine disruptor compounds. However, environmentally significant concentrations are
still present in the treated effluent. The results also show that enzyme-linked immunosorbent assay kit is suitable for routine
analysis of the selected compounds. The results are also useful since the wastewater treatment plant is located in a Mediterranean
region, which results in an effluent with its own characteristics. 相似文献
36.
Abstact The present study was designed to evaluate indirect, non-invasive, on-line measurement of biofilm thickness using an electrical capacitance technique. Several assays were carried out and the results showed that, at a frequency of 1 kHz, electrical capacitance could be used to measure biofilm thickness indirectly (with a correlation coefficient of 0.9495). The reproducibility revealed by the assays was also highly satisfactory.However, in contrast to what was expected, there was an inverse relationship between electrical capacitance and biofilm thickness, i.e. electrical capacitance diminished with the increase in the biofilm thickness.The tests were also carried out at different frequencies (1 kHz, 10 kHz and 100 kHz) 相似文献
37.
de Carvalho George Harrison Ferreira de Andrade Milene Aparecida de Araújo Carla Nunes Santos Maria Lucília de Castro Natália Alves Charneau Sébastien Monnerat Rose de Santana Jaime Martins Bastos Izabela Marques Dourado 《Environmental science and pollution research international》2019,26(6):5514-5523
Environmental Science and Pollution Research - Aedes aegypti and Culex quinquefasciatus are vectors of diseases that constitute public health problems. The discovery of products capable of... 相似文献
38.
Zhigao Sun Xiaojie Mou 《Environmental science and pollution research international》2016,23(6):5189-5202
From April 2008 to November 2009, a field decomposition experiment was conducted to investigate the effects of sediment burial on macro (C, N) and microelement (Pb, Cr, Cu, Zn, Ni, and Mn) variations in decomposing litter of Phragmites australis in the coastal marsh of the Yellow River estuary. Three one-off sediment burial treatments [no sediment burial (0 mm year?1, S0), current sediment burial (100 mm year?1, S10), and strong sediment burial (200 mm year?1, S20)] were laid in different decomposition sites. Results showed that sediment burials showed significant influence on the decomposition rate of P. australis, in the order of S10 (0.001990 day?1)?≈?S20 (0.001710 day?1)?>?S0 (0.000768 day?1) (p?<?0.05). The macro and microelement in decomposing litters of the three burial depths exhibited different temporal variations except for Cu, Zn, and Ni. No significant differences in C, N, Pb, Cr, Zn, and Mn concentrations were observed among the three burial treatments except for Cu and Ni (p?>?0.05). With increasing burial depth, N, Cr, Cu, Ni, and Mn concentrations generally increased, while C, Pb, and Zn concentrations varied insignificantly. Sediment burial was favorable for C and N release from P. australis, and, with increasing burial depth, the C release from litter significantly increased, and the N in litter shifted from accumulation to release. With a few exceptions, Pb, Cr, Zn, and Mn stocks in P. australis in the three treatments evidenced the export of metals from litter to environment, and, with increasing burial depth, the export amounts increased greatly. Stocks of Cu and Ni in P. australis in the S10 and S20 treatments were generally positive, evidencing incorporation of the two metals in most sampling times. Except for Ni, the variations of C, N, Pb, Cr, Cu, Zn, and Mn stocks in P. australis in the S10 and S20 treatments were approximated, indicating that the strong burial episodes (S20) occurred in P. australis marsh in the future would have little influence on the stocks of these elements. With increasing burial depths, the P. australis was particularly efficient in binding Cu and Ni and releasing C, N, Pb, Cr, Zn, and Mn, implying that the potential eco-toxic risk of Pb, Cr, Zn, and Mn exposure might be very serious. This study emphasized the effects of different burials on nutrient and metal cycling and mass balance in the P. australis marsh of the Yellow River estuary. 相似文献
39.
40.
Sabine Delannoy Lothar Beutin Patrick Fach 《Environmental science and pollution research international》2016,23(9):8163-8174
Among strains of Shiga-toxin-producing Escherichia coli (STEC), seven serogroups (O26, O45, O103, O111, O121, O145, and O157) are frequently associated with severe clinical illness in humans. The development of methods for their reliable detection from complex samples such as food has been challenging thus far, and is currently based on the PCR detection of the major virulence genes stx1, stx2, and eae, and O-serogroup-specific genes. However, this approach lacks resolution. Moreover, new STEC serotypes are continuously emerging worldwide. For example, in May 2011, strains belonging to the hitherto rarely detected STEC serotype O104:H4 were identified as causative agents of one of the world’s largest outbreak of disease with a high incidence of hemorrhagic colitis and hemolytic uremic syndrome in the infected patients. Discriminant typing of pathogens is crucial for epidemiological surveillance and investigations of outbreaks, and especially for tracking and tracing in case of accidental and deliberate contamination of food and water samples. Clustered regularly interspaced short palindromic repeats (CRISPRs) are composed of short, highly conserved DNA repeats separated by unique sequences of similar length. This distinctive sequence signature of CRISPRs can be used for strain typing in several bacterial species including STEC. This review discusses how CRISPRs have recently been used for STEC identification and typing. 相似文献