Diet of the Humboldt penguin (<Emphasis Type="Italic">Spheniscus humboldti</Emphasis>) in northern and southern Chile

The diet of the Humboldt penguin (Spheniscus humboldti) was examined and compared in two colonies in Chile. Field work was conducted on Pan de Azúcar Island in northern Chile in the breeding season 1998/1999 and on the Puñihuil Islands in southern Chile over two successive breeding seasons during 1997/1998 and 1998/1999. Penguin diet was studied by stomach-pumping birds and analysed by species composition, size and mass of prey. Fish were the dominant prey item at both sites, the contribution of cephalopods and crustaceans varying between sites. The fish prey consisted predominantly of school fish, but there were clear latitudinal differences in fish prey taken. Penguins in the northern colony consumed primarily garfish (Scomberesox saurus), while birds at the southern colony of Puñihuil fed primarily on anchovy (Engraulis ringens), Araucanian herring (Strangomera bentincki) and silverside (Odontesthes regia). The results showed significant differences in terms of numbers of fish taken between the two breeding seasons at Puñihuil. In 1997/1998 penguins consumed almost exclusively anchovy, while they fed primarily on silversides in the successive year. Almost all prey, except stomatopods, were characterised as being pelagic species that occur in relatively inshore water, consistent with the foraging behaviour of Humboldt penguins. The dependence of Humboldt penguins on commercially exploited, schooling prey species makes the species particularly susceptible to changes in prey stocks, due to non-sustainable fisheries management.Communicated by O. Kinne, Oldendorf/Luhe     

Superoxide production by marine microalgae

A survey was conducted for production of the reactive oxygen species superoxide by 37 species (65 strains) of microalgae including dinoflagellates, raphidophytes, chlorophytes, prasinophytes, eustigmatophytes and prymnesiophytes. Ichthyotoxic raphidophyte species of Chattonella were found to produce the highest environmental levels of superoxide (177×10⁴ total chemiluminescence units). However, ichthyotoxic dinoflagellates (Karenia, Alexandrium) and the prymnesiophyte Prymnesium were also found to produce significant levels of superoxide (4×10⁴, 3×10⁴ and 5×10⁴ chemiluminescence units, respectively), equivalent to that of other raphidophyte species of Heterosigma and Fibrocapsa (6×10⁴ and 2×10⁴, respectively). A direct relationship between cell size and superoxide production was observed (r²=0.94), with larger cells producing more superoxide per cell. Chattonella produced the most superoxide per cell (expressed as cellular chemiluminescence units), followed by the dinoflagellate species Karenia, Alexandrium, Takayama and Gymnodinium. Small cells, such as the raphidophyte Heterosigma and the prymnesiophyte Prymnesium produced very little superoxide per cell (cellular chemiluminescence units), but potentially could still produce high total levels of superoxide if present at high biomass levels. Species commonly used as aquaculture bivalve feeds such as Dunaliella, Tetraselmis, Nannochloropsis and Pavlova produced negligible levels of superoxide, even at high biomass. We speculate that superoxide, while not the sole ichthyotoxic principle, may play a wider role in algal toxicity than previously considered, and propose a broad classification of microalgae based upon superoxide production.Communicated by M.S. Johnson, Crawley     

Influence of soil properties on bioaccumulation of 14C-simazine in earthworms Eisenia foetida

The toxicity of pesticides has been evaluated by several methods including tests with earthworms in both artificial and natural soils treated with the compounds. The ecological niches of earthworms make them good bioindicators of soil contamination. The bioaccumulation of 14C-simazine (6-chloro-N2-N4-diethyl- 1,3,5-triazine-2,4-diamine) was evaluated in earthworms (Eisenia foetida) maintained during three months in two substrates with different physical-chemical characteristics. The substrates were treated with 3.0 mg and 330 kBq of 14C-simazine kg(-1) substrate. Results indicated that worms did not influence simazine dissipation in both substrates as indicated by similar recoveries and with no statistical differences with and without earthworms. The radiocarbon recoveries were 86.8 and 95.3%, respectively in the substrates with lower and higher organic matter contents with earthworms, and 91.0 and 107.4% in the same substrates without worms. However, in earthworms the recoveries were statistically higher when they were maintained in the substrate with lower amount of organic matter (0.89%) than from the higher one (0.33%). Consequently, 14C-simazine bioconcentration factor (BCF) was also greater in the substrate with lower organic matter (6.89+/-1.55) than in the substrate with higher organic matter content (0.88+/-0.06). The results suggest that the higher soil organic matter content will cause lower probability of contamination of soil organisms with simazine.     

Trace elements in biodeposits and sediments from mussel culture in the Ria de Arousa (Galicia, NW Spain)

The trace elements present at highest concentrations were Cr and Zn, which probably originated from the dumping of effluent from a tanning factory. High proportions of these two elements were associated with the residual fraction. Biodeposits and sediments showed high concentrations of Cd and Pb in the reactive fraction, with a high proportion of the concentration in the reactive fraction being associated with carbonates. Nickel showed a higher degree of pyritization than the previous elements, although most of the Ni was associated with the residual and reactive fractions. Arsenic, Hg and Cu showed high degrees of pyritization, particularly below a depth of 5 cm. The results demonstrate that those elements with a high degree of pyritization may be released into the water through oxidation of the metal sulphides that they form when in suspension in oxic sea water, with the subsequent risk of increased bioavailability to benthic fauna.     

Effects of arbuscular mycorrhizal fungi on the root uptake and translocation of radiocaesium

Because mycorrhizal fungi are intimately associated with plant roots, their importance in radionuclide (RN) recycling and subsequent dispersion into the biosphere has received an increasing interest. Recently, the capacity of arbuscular mycorrhizal fungi to take up and translocate radiocaesium to their host was demonstrated. However, the relative contribution of these processes in comparison to the ones of roots remains unknown. Here, the respective contributions of the hyphae of a Glomus species and the transformed carrot (Daucus carota L.) roots on radiocaesium uptake and translocation were compared and quantified. We observed that radiocaesium uptake by hyphae was significantly lower as compared to that of the roots, while the opposite was noted for radiocaesium translocation/uptake ratio. We also observed that the intraradical fungal structures might induce a local accumulation of radiocaesium and concurrently reduce its translocation within mycorrhizal roots. We believe that intraradical fungal structures might induce the down-regulation of radiocaesium channels involved in the transport processes of radiocaesium towards the xylem.     

Ecotoxicological assessment for receiving waters with the premetamorphic tadpoles acute assay

The toxicity of receiving waters was evaluated by means of the young tadpoles assays. The sentinel organism was Bufo arenarum, an indigenous anuran species. The assayed water samples were taken from a highly polluted urban watercourse (Reconquista River, Buenos Aires, Argentina), characterized by high concentration of nitrites, phosphates and heavy metals. The toxicity of water samples was assessed performing the pass-fail test and by means of the NOEC and LC(50); TUs (toxic units) were also calculated. The effect of the addition of a positive control (Cd) on the toxicity of the river water samples was also examined. Water samples of three sites, characterised by their different degree of pollution, were assayed. The lethal response had a clear cut correspondence with the water quality of the sample evaluated by means of physicochemical parameters. In most cases, the dilution of the samples resulted in a significant reduction of their toxicity to TU values compatible to those stated by the USEPA for industrial effluents. It was concluded that (a) the used Bufo arenarum bioassay was an adequate method for assessing the toxicity of natural polluted water samples and (b) the three considered endpoints showed no important differences after 48 or 96 h of exposure, therefore we concluded that it may not be necessary to extend the assay for a longer period than 48 h.     

Mitigation of a radon-rich Belgian dwelling using active subslab depressurization

In a radon prone area in Belgium, a dwelling with high indoor radon concentrations was identified through a passive measurement. Next, a continuous, active radon monitoring device was installed for one month. A 20-a retrospective radon assessment was also performed. The house was subsequently mitigated through active subslab depressurization with a radial fan. Afterwards the dwelling was actively monitored for several more months to observe the effects of the mitigation and to study the effect of reducing the fan power. Dose evaluations were made to evaluate the health benefit of the mitigation. It was seen that the results of the three measuring techniques before mitigation all yielded between 1700 and 2000 Bq/m3. Clear diurnal radon variations showed up only after mitigation. After mitigation, the average radon concentration fell to less than 200 Bq/m3. The yearly average dose was reduced from potentially 45 mSv/y to less than 4.5 mSv/y through mitigation. Reducing fan power to 50% did not clearly influence the amount of radon entering into the dwelling.