Accumulation of trinitrotoluene (TNT) in aquatic organisms: part 2--Bioconcentration in aquatic invertebrates and potential for trophic transfer to channel catfish (Ictalurus punctatus)

The potential of TNT to accumulate in aquatic organisms was assessed by determining bioconcentration factors for TNT and TNT biotransformation products using two benthic invertebrates (Chironomus tentans and Lumbriculus variegatus), and by determining the bioaccumulation factor of TNT and TNT biotransformation products due to TNT exposure via feeding for channel catfish (Ictalurus punctatus). In all three species, TNT was rapidly biotransformed resulting in minimal accumulation. The bioconcentration factors for parent TNT ranged from 3 to 4 ml g(-1) for the invertebrates studied, while the TNT bioaccumulation factor for catfish via oral exposure of food pellets was 2.4x10(-5) g g(-1) based on the concentration of TNT in the food pellet. As indicated by this small bioaccumulation factor, TNT accumulation in channel catfish through trophic transfer would be negligible compared to aqueous exposure (previously reported BCF of 0.79 ml g(-1)). TNT extractable biotransformation products accumulated to a greater degree than parent TNT for all three species. In addition, a large fraction of the radioactivity within all three species resisted solvent extraction. The highest bioconcentration factors occurred in L. variegatus with extractable radioactivity measuring 76 ml g(-1) and total radioactivity measuring 216 ml g(-1). Because the bioaccumulation of TNT is very low compared to the bioaccumulation of its biotransformation products, further research including identifying and determining the relative toxicities of these biotransformation products is necessary to fully evaluate the environmental risk posed by exposure to TNT.     

Assessment of the Water Quality and Ecosystem Health of the Great Barrier Reef (Australia): Conceptual Models

Run-off containing increased concentrations of sediment, nutrients, and pesticides from land-based anthropogenic activities is a significant influence on water quality and the ecologic conditions of nearshore areas of the Great Barrier Reef World Heritage Area, Australia. The potential and actual impacts of increased pollutant concentrations range from bioaccumulation of contaminants and decreased photosynthetic capacity to major shifts in community structure and health of mangrove, coral reef, and seagrass ecosystems. A detailed conceptual model underpins and illustrates the links between the main anthropogenic pressures or threats (dry-land cattle grazing and intensive sugar cane cropping) and the production of key contaminants or stressors of Great Barrier Reef water quality. The conceptual model also includes longer-term threats to Great Barrier Reef water quality and ecosystem health, such as global climate change, that will potentially confound direct model interrelationships. The model recognises that system-specific attributes, such as monsoonal wind direction, rainfall intensity, and flood plume residence times, will act as system filters to modify the effects of any water-quality system stressor. The model also summarises key ecosystem responses in ecosystem health that can be monitored through indicators at catchment, riverine, and marine scales. Selected indicators include riverine and marine water quality, inshore coral reef and seagrass status, and biota pollutant burdens. These indicators have been adopted as components of a long-term monitoring program to enable assessment of the effectiveness of change in catchment-management practices in improving Great Barrier Reef (and adjacent catchment) water quality under the Queensland and Australian Governments’ Reef Water Quality Protection Plan.     

Toxicity and attraction effects produced byMorinda citrifolia fruits on theDrosophila melanogaster complex of species

Summary The ripe fruit of the Indian mulberry,Morinda citrifolia, is the host plant forDrosophila sechellia but is highly toxic for three closely related species (D. melanogaster, D. simulans, D. mauritiana). A simple bioassay is described with which a clear dose response to the fruit was found for these species. Significant differences in reactivity to the ripe fruit were found among species. Tested strains ofD. simulans andD. mauritiana adults were more sensitive to the toxic properties of the fruit thanD. melanogaster. A marked intraspecific variability was shown inD. melanogaster. Reciprocal interspecific hybridizations betweenD. sechellia andD. mauritiana suggested an autosomal dominant control of resistance. MoreoverD. melanogaster intraspecific crossings suggested the influence of an additional X-linked factor. Responses of flies toMorinda fruit in different states were tested in a T olfactometer. The less resistant strains ofDrosophila generally showed less preference for the ripe fruit.     

Effects of Clear-Cut Harvesting on Boreal Ground-Beetle Assemblages (Coleoptera: Carabidae) in Western Canada

We compared pitfall catches from four types of mature lodgepole pine—white spruce forest with those from five age classes of young forest regenerating subsequent to clear-cutting. Ground beetles were most abundant in the youngest sites (1–2 years since cutting) and in the mature stands on moist soil. Species richness was higher in regenerating sites than in mature forest. Cluster analysis grouped the ground-beetle fauna according to forest age and forest type, suggesting that there is a general pattern of recovery after logging. Responses of common species to forest cutting fell into three groups: (1) forest generalists (2 species) were not dramatically affected, (2) species of open habitat (27 species) appeared and/or increased in abundance, and (3) mature forest species (10 species) disappeared or decreased in abundance. Populations of many mature forest species appear to recover following logging, but several specialists did not recolonize even the oldest regenerating stands. Furthermore, fragmentation and creation of large areas of relatively homogeneous young forest stages through logging may have detrimental long-term effects even on the more abundant forest generalists. We must better understand subtle variations in habitat in order to maintain invertebrate diversity while harvesting the boreal forest.     

A preliminary in vitro assessment of GroBiotic-A, brewer's yeast and fructooligosaccharide as prebiotics for the red drum Sciaenops ocellatus

This study examined the effects of brewers yeast, fructooligosaccharide (FOS), and GroBiotic-A, a mixture of partially autolyzed brewers yeast, dairy components and dried fermentation products, on the intestinal microbial community of red drum, Sciaenops ocellatus. Gastrointestinal (GI) tracts were aseptically removed from three sub-adult red drum previously maintained on a commercial diet and placed in an anaerobic chamber. Intestinal contents were removed, diluted and incubated in vitro in one of four liquid media: normal diet alone, diet + 2% (w/w) GroBiotic-A, diet + 2% brewers yeast, and diet + 2% FOS. After 24 and 48 h of incubation at 25 degrees C, supernatants were removed for volatile fatty acid (VFA) analysis and DNA was extracted for denaturing gradient gel electrophoresis (DGGE) analysis. Polymerase chain reaction (PCR) was performed on a highly conserved region of M 16S rDNA and the amplicons were subjected to DGGE. The microbial community (MC) fingerprint was used to distinguish microbial populations. The intestinal contents incubated with GroBiotic-A had significantly (P<0.05) higher acetate and total VFA concentrations at 48 h compared to the other treatments. DGGE analysis demonstrated that the microbial community was significantly altered by Grobiotic-A and brewers yeast.     

Importance of information on tillage practices in the modelling of environmental processes and in the use of environmental indicators

Tillage has been and will always be integral to crop production. Tillage can result in the degradation of soil, water, and air quality. Of all farm management practices, tillage may have the greatest impact on the environment. A wide variety of tillage equipment, practices and systems are available to farmers, providing opportunities to enhance environmental performance. These opportunities have made tillage a popular focus of environmental policies and programs such as environmental indicators for agriculture. This paper provides a very brief examination of the role of tillage in crop production, its effect on biophysical processes and, therefore, its impact on the environment. Models of biophysical processes are briefly examined to demonstrate the importance of tillage relative to other farm management practices and to demonstrate the detail of tillage data that these models can demand. The focus of this paper is an examination of the use of information on tillage in Canada's agri-environmental indicators initiative, National Agri-environmental Health Analysis and Reporting Program (NAHARP). Information on tillage is required for several of the indicators in NAHARP. The type of data used, its source, and its quality are discussed. Recommendations regarding the collection of tillage data and use of tillage information are presented.     

Urban waterfowl population: Ecological evaluation of management and planning

An urban population of ducks in Puyallup, Washington, USA was studied for 14 consecutive months beginning in November 1978. Observations were made weekly from four study sites where ducks would congregate at early morning hours. Factors contributing to the presence of waterfowl in Puyallyup included abundant food supplies and a creek corridor that connected fragmented habitats in the urban area to the larger rural populations of waterfowl. Mallards (Anas platyrhynchos) were the most abundant of the 13 species observed and were the only ducks remaining during the nesting season. Habitat size and complexity were important factors influencing the species diversity of a particular site. Nesting success of mallards was poor due to limited distribution of nesting habitat, intraspecific aggression, and human disturbance. Both site-specific and more broad-based strategies are suggested for managing and planning for duck populations in urban areas.     

THE DEFINITION AND MEASUREMENT OF MINERAL CONSUMPTION

The concept of mineral consumption is considered in the context of a (metallic) commodity's progression from mine to manufacture and reasons are advanced as to why consumption is best measured at the point referred to as the “first processing stage.” Two methods for measuring consumption at this stage, one direct (“reported consumption”) and one deductive (“apparent consumption”), are described and the merits and drawbacks of the methods discussed. The article concludes with a brief review of the existing statistics on worldwide mineral consumption and suggests that there is the scope, and the need, for improvement. Dans cet article, la notion de la consommation minérale est envisagée dans le cadre de l'acheminement du minerai (métallique) de la mine à l'usine de transformation. II donne les raisons pour lesquelles la consommation peut se mesurer le mieux au niveau de la “phase de première transformation.” A ce stade, l'article décrit deux méthodes permettant de mesurer cette consommation: l'une directe (la consommation déclarée) et l'autre supposée (la consommation apparente). Les avantages et les inconvénients des méthodes y sont aussi discutés. En conclusion, l'article passe brièvement en revue les statistiques existantes sur la consommation minérale mondiale et suggère que la possibilité ainsi que le besoin d'amélioration se font sentir dans ce domaine. El concepto de consumo de minerales se examina dentro del contexto de la progresión del producto desde la mina a la manufactura y se sostiene que dicho consumo se mide mejor en la “etapa de primer procesamiento.” Se describen dos métodos para medir el consumo en esta etapa, el método directo (consumo reportado) y deductivo (consumo aparente). Se discuten las ventajas y desventajas de estos métodos. El artículo concluye con una revisión breve de las estadísticas existentes del consumo de minerales a nivel mundial y sugiere que existe la necesidad y la posibilidad para mejorarlas.